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41.
Moore CA Bowen HC Scrase-Field S Knight MR White PJ 《The Plant journal : for cell and molecular biology》2002,30(4):457-465
A transient increase in cytosolic Ca2+ concentration ([Ca2+]cyt) is thought to be a prerequisite for an appropriate physiological response to both chilling and salt stress. The [Ca2+]cyt is raised by Ca2+ influx to the cytosol from the apoplast and/or intracellular stores. It has been speculated that different signals mobilise Ca2+ from different stores, but little is known about the origin(s) of the Ca2+ entering the cytosol in response to specific environmental challenges. We have utilised the developmentally regulated suberisation of endodermal cells, which is thought to prevent Ca2+ influx from the apoplast, to ascertain whether Ca2+ influx is required to increase [Ca2+]cyt in response to chilling or salt stress. Perturbations in [Ca2+]cyt were studied in transgenic Arabidopsis thaliana, expressing aequorin fused to a modified yellow fluorescent protein solely in root endodermal cells, during slow cooling of plants from 20 to 0.5 degrees C over 5 min and in response to an acute salt stress (0.333 m NaCl). Only in endodermal cells in the apical 4 mm of the Arabidopsis root did [Ca2+]cyt increase significantly during cooling, and the magnitude of the [Ca2+]cyt elevation elicited by cooling was inversely related to the extent of suberisation of the endodermal cell layer. No [Ca2+]cyt elevations were elicited by cooling in suberised endodermal cells. This is consistent with the hypothesis that suberin lamellae isolate the endodermal cell protoplast from the apoplast and, thereby, prevent Ca2+ influx. By contrast, acute salt stress increased [Ca2+]cyt in endodermal cells throughout the root. These results suggest that [Ca2+]cyt elevations, upon slow cooling, depend absolutely on Ca2+ influx across the plasma membrane, but [Ca2+]cyt elevations in response to acute salt stress do not. They also suggest that Ca2+ release from intracellular stores contributes significantly to increasing [Ca2+]cyt upon acute salt stress. 相似文献
42.
Silicon deposition in the root reduces sodium uptake in rice (Oryza sativa L.) seedlings by reducing bypass flow 总被引:2,自引:0,他引:2
Sodium chloride reduces the growth of rice seedlings, which accumulate excessive concentrations of sodium and chloride ions in their leaves. In this paper, we describe how silicon decreases transpirational bypass flow and ion concentrations in the xylem sap in rice (Oryza sativa L.) seedlings growing under NaCl stress. Salt (50 mM NaCl) reduced the growth of shoots and roots: adding silicate (3 mM) to the saline culture solution improved the growth of the shoots, but not roots. The improvement of shoot growth in the presence of silicate was correlated with reduced sodium concentration in the shoot. The net transport rate of Na from the root to shoot (expressed per unit of root mass) was also decreased by added silicate. There was, however, no effect of silicate on the net transport of potassium. Furthermore, in salt-stressed plants, silicate did not decrease the transpiration, and even increased it in seedlings pre-treated with silicate for 7 d prior to salt treatment, indicating that the reduction of sodium uptake by silicate was not simply through a reduction in volume flow from root to shoot. Experiments using trisodium-8-hydroxy-1,3,6-pyrenetrisulphonic acid (PTS), an apoplastic tracer, showed that silicate dramatically decreased transpirational bypass flow in rice (from about 4.2 to 0.8%), while the apparent sodium concentration in the xylem, which was estimated indirectly from the flux data, decreased from 6.2 to 2.8 mM. Direct measurements of the concentration of sodium in xylem sap sampled using Philaenus spumarius confirmed that the apparent reduction was not a consequence of sodium recycling. X-ray microanalysis showed that silicon was deposited in the outer part of the root and in the endodermis, being more obvious in the latter than in the former. The results suggest that silicon deposition in the exodermis and endodermis reduced sodium uptake in rice (Oryza sativa L.) seedlings under NaCl stress through a reduction in apoplastic transport across the root. 相似文献
43.
BACKGROUND AND AIMS: Free-hand sectioning of living plant tissues allows fast microscopic observation of internal structures. The aim of this study was to improve the quality of preparations from roots with suberized cell walls. A whole-mount procedure that enables visualization of exo- and endodermal cells along the root axis was also established. METHODS: Free-hand sections were cleared with lactic acid saturated with chloral hydrate, and observed with or without post-staining in toluidine blue O or aniline blue. Both white light and UV light were used for observation. Lactic acid was also used as a solvent for berberine, and fluorol yellow for clearing and staining the samples used for suberin observation. This procedure was also applied to whole-mount roots with suberized celllayers. KEY RESULTS: Clearing of sections results in good image quality to observe the tissue structure and cell walls compared with non-cleared sections. The use of lactic acid as a solvent for fluorol yellow proved superior to previously used solvents such as polyethylene glycol-glycerol. Clearing and fluorescence staining of thin roots such as those of Arabidopsis thaliana were successful for suberin visualization in endodermal cells within whole-mount roots. For thicker roots, such as those of maize, sorghum or tea, this procedure could be used for visualizing the exodermis in a longitudinal view. Clearing and staining of peeled maize root segments enabled observation of endodermal cell walls. CONCLUSIONS: The clearing procedure using lactic acid improves the quality of images from free-hand sections and clearings. This method enhances the study of plant root anatomy, in particular the histological development and changes of cell walls, when used in combination with fluorescence microscopy. 相似文献
44.
以蚕豆(ViciafabaL.)幼苗为材料,结合不同的光强及黑暗对茎中内皮层形成的影响,对参与栓化作用有关的酶活性及内源抗氧化物质的含量进行了测定,并讨论了光对内皮层形成的调节机制。实验结果表明,高光强抑制茎中内皮层形成,而低光强和黑暗诱导茎中形成内皮层。在低光强和暗中,脂肪氧化酶在内皮层栓化前期活性显著增加,可能与栓化作用的启动有关,而高光强下LOX始终处在较低水平。PAL活性为光所诱导,与内皮层的栓化没有直接的相关性。在高光强条件下生长的幼苗茎中抗坏血酸和谷胱甘肽含量比低光强和暗中的高。外源施用抗坏血酸,可抑制低光强下茎内皮层的栓化,而对暗中茎内皮层的栓化无影响。推测高强度光对栓化作用的抑制原因可能是高光强下植物体内有高含量的抗氧化物质,并具备更有利的活性氧清除途径,从而抑制了栓化作用的进行 相似文献
45.
M. Damus R. L. Peterson Daryl E. Enstone Carol A. Peterson 《Plant biology (Stuttgart, Germany)》1997,110(2):190-195
Forty-three species of seedless vascular plants were assessed for modifications to root cortical cell walls. All species except Lycopodium had an endodermis with distinct Casparian bands. Experiments with the apoplastic tracer berberine hemisulfate showed that walls of all root cortical cells in the two Lycopodium species tested were permeable to this tracer. Although most species examined lacked a hypodermis several Equisetum species had a hypodermis with modified walls. Three Selaginella species had distinct Casparian bands in this cortical cell layer. This layer, therefore, is an exodermis in Selaginella and its presence limited the inward diffusion of the apoplastic tracer berberine hemisulfate. 相似文献
46.
Van Steveninck R. F. M. Babare A. Fernando D. R. Van Steveninck M. E. 《Plant and Soil》1993,(1):525-528
Appreciable quantities of Zn are bound as Zn phytate (myo-inositol kis-hexaphosphate) within small vacuoles of cortical cells in the elongation zone of root tips of zinc tolerant Deschampsia caespitosa. These Zn/P-containing globular deposits have now been shown to occur in the roots of soybean, lucerne, lupins, tomato, rapeseed, cabbage, radish, wheat and maize. The globules are most frequent in the endodermis and pericycle but may also occur in the stele and inner cortex. The X-ray data again confirmed the presence of phytate with a relatively stable proportion of Zn and a species-dependent, variable, proportion of K, Mg and Ca to P.Analysis of soybean plants by atomic absorption spectroscopy showed that the Zn concentration in the shoots doubled in response to an increase in Zn supply from 1 to 100 M while the concentration of Zn in the root symplast was approximately 22 times greater than in the shoot, suggesting restricted transport to the shoot. It is suggested that the genetic expression of the capacity to bind heavy metals by means of phytate in endodermal cells may provide a strategy for keeping the above-ground content of heavy metals low. It may be possible to incorporate the trait into transformed roots that can be utilized for the treatment of industrial wastes. 相似文献
47.
48.
Alexander Lux Miroslava Luxová Jun Abe Shigenori Morita Shinobu Inanaga 《Plant and Soil》2003,255(1):85-91
Bamboo is a silicon accumulating plant. In leaves, the major place of silicon (Si) deposition is the epidermis, with the highest concentration of Si in silica cells. In bamboo roots, the deposition of Si is found only in endodermal cell walls. The silicification of leaves and roots was examined in the economically important bamboo plant Phyllostachys heterocycla, using an environmental scanning electron microscope coupled with X-ray microanalysis, as well as gravimetric quantification. The content of Si on a dry weight basis measured by gravimetric quantification was 7.6% in leaves and 2.4% in roots, respectively. Moreover, quantification of EDX data showed high Si impregnation of the inner tangential endodermal walls. Si content in this part of the root endodermal cell walls was even higher than that in the outer leaf epidermal walls, where conspicuous deposition of Si often occurs in grass plants. 相似文献
49.
50.
Rates of diffusion into roots of maize 总被引:1,自引:1,他引:0