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31.
Aims: A total of nine Listeria monocytogenes strains (seven serotypes) were studied to ascertain whether the non-steroidal anti-inflammatory drug diclofenac (Dc) used in combination with the conventional antilisterial antibiotic gentamicin (Gm) or ampicillin (Am) synergistically augments the efficacy of the antibiotic in vitro .
Methods and Results: The effect of combination was evaluated by the checkerboard method to obtain a fractional inhibitory concentration (FIC) index followed by kill curves. Dc was synergistic with Gm (FIC 0·37) and there was indifference with Am (FIC 1) against L. monocytogenes ATCC 51774. The magnitude of the differences between killing by a single agent and the combination observed at 24 h was significant ( P < 0·05) for Dc plus Gm but not Dc plus Am.
Conclusions: Thus, the ability of extended antibiotic therapy may be improved with the help of this synergistic drug pair in listeriosis.
Significance and Impact of the Study: Such findings may indicate parallel administration of anti-inflammatory and anti listeriosis drugs. 相似文献
Methods and Results: The effect of combination was evaluated by the checkerboard method to obtain a fractional inhibitory concentration (FIC) index followed by kill curves. Dc was synergistic with Gm (FIC 0·37) and there was indifference with Am (FIC 1) against L. monocytogenes ATCC 51774. The magnitude of the differences between killing by a single agent and the combination observed at 24 h was significant ( P < 0·05) for Dc plus Gm but not Dc plus Am.
Conclusions: Thus, the ability of extended antibiotic therapy may be improved with the help of this synergistic drug pair in listeriosis.
Significance and Impact of the Study: Such findings may indicate parallel administration of anti-inflammatory and anti listeriosis drugs. 相似文献
32.
Synthetic antimicrobial β‐peptide in dual‐treatment with fluconazole or ketoconazole enhances the in vitro inhibition of planktonic and biofilm Candida albicans 下载免费PDF全文
Camilo Mora‐Navarro Jean Caraballo‐León Madeline Torres‐Lugo Patricia Ortiz‐Bermúdez 《Journal of peptide science》2015,21(12):853-861
Fungal infections are a pressing concern for human health worldwide, particularly for immunocompromised individuals. Current challenges such as the elevated toxicity of common antifungal drugs and the emerging resistance towards these could be overcome by multidrug therapy. Natural antimicrobial peptides, AMPs, in combination with other antifungal agents are a promising avenue to address the prevailing challenges. However, they possess limited biostability and susceptibility to proteases, which has significantly hampered their development as antifungal therapies. β‐peptides are synthetic materials designed to mimic AMPs while allowing high tunability and increased biostability. In this work, we report for the first time the inhibition achieved in Candida albicans when treated with a mixture of a β‐peptide model and fluconazole or ketoconazole. This combination treatment enhanced the biological activity of these azoles in planktonic and biofilm Candida, and also in a fluconazole‐resistant strain. Furthermore, the in vitro cytotoxicity of the dual treatment was evaluated towards the human hepatoma cell line, HepG2, a widely used model derived from liver tissue, which is primarily affected by azoles. Analyses based on the LA‐based method and the mass‐action law principle, using a microtiter checkerboard approach, revealed synergism of the combination treatment in the inhibition of planktonic C. albicans. The dual treatment proved to be fungicidal at 48 and 72 h. Interestingly, it was also found that the viability of HepG2 was not significantly affected by the dual treatments. Finally, a remarkable enhancement in the inhibition of the highly azole‐resistant biofilms and fluconazole resistant C. albicans strain was obtained. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
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Cellobiohydrolase Cel48C from Paenibacillus sp. BP-23, an enzyme displaying limited activity on most cellulosic substrates, was assayed for activity in the presence of other bacterial endo- or exocellulases. Significant enhanced activity was observed when Cel48C was incubated in the presence of Paenibacillus sp. BP-23 endoglucanase Cel9B or Thermobifida fusca cellulases Cel6A and Cel6B, indicating that Cel48C acts synergistically with them. Maximum synergism rates on bacterial microcrystalline cellulose or filter paper were obtained with a mixture of Paenibacillus cellulases Cel9B and Cel48C, accompanied by T. fusca exocellulase Cel6B. Synergism was also observed in cell extracts from recombinant clone E. coli pUCel9-Cel48 expressing the two contiguous Paenibacillus cellulases Cel9B and Cel48C. The enhanced cellulolytic activity displayed by the cellulase mixtures assayed could be used as an efficient tool for biotechnological applications like pulp and paper manufacturing. 相似文献
35.
印楝油对印楝素的增效作用和保护作用 总被引:3,自引:0,他引:3
印楝油对印楝素具有增效作用,印楝素A和印楝种子石油醚抽提物和印楝种子植物油分别按1:10(m/m)混用处理斜纹夜蛾3龄幼虫,共毒系数分别为150.17和226.87,印楝素B和两者混用后的共毒系数分别为166.00和242.18。印楝种子植物油对印楝素具有保护作用,在54℃下热贮7d,印楝油(甲醇:印楝油=9-1,v/v)对印楝素A和印楝素B的保护效果分别为56.96%和50.86%;热贮14d,保护效果分别为26.72%和38.42%。 相似文献
36.
HaSNPV几丁质酶基因在大肠杆菌和昆虫细胞中的表达及其产物对Bt杀虫剂的增效作用 总被引:4,自引:0,他引:4
为了探索杆状病毒几丁质酶对微生物杀虫剂的增效作用及其利用途径 ,分别在大肠杆菌和昆虫细胞中表达棉铃虫单粒包埋型核型多角体病毒 (HaSNPV)几丁质酶 .用PCR方法扩增出不含N端信号肽编码序列的几丁质酶基因片段 ,并分别克隆至原核表达载体pET2 8a和重组到杆状病毒BactoBac表达系统 ,在大肠杆菌 (E .coli)BL2 1和粉纹夜蛾 (Trichoplusiani)细胞系Tn 5B1 4中分别进行了表达 .在大肠杆菌中表达量约占细菌总蛋白 15 % ,在昆虫细胞中表达量约占细胞总蛋白10 % .将含有几丁质酶的大肠杆菌和昆虫细胞表达产物添加到苏云金杆菌 (Bt)菌液中一起喂食 2龄家蚕 .结果显示 ,HaSNPV几丁质酶基因的 2种表达产物和Bt杀虫剂的混合物使处理的家蚕的致死时间较对照处理均明显缩短 .昆虫细胞和大肠杆菌表达产物与Bt混合物处理的LT50 分别从 93 5h和 95 1h缩短到 5 6 2h及 6 7 2h ,并且供试家蚕的生长速度明显缓慢 .研究结果表明 ,重组的HaSNPV几丁质酶有望作为Bt杀虫剂的增效剂 相似文献
37.
马铃薯Y病毒蚜传辅助成分介导PVX/PVY协生作用 总被引:4,自引:0,他引:4
构建了马铃薯Y病毒中国株系(PVY-C)蚜传辅助成分(HC-Pro)基因的正义、反义和缺失三种植物表达载体,通过农杆菌介导法转化烟草品种NC89。Southern blot分析表明,HC-Pro基因及其突变体已经整合到烟草染色体中,Western blot分析证明,正义HC-Pro基因及其缺失突变体在转基因烟草中有表达产物,攻毒试验结果表明,转正义,HC-Pro基因及其缺失突变体不仅能够提高T1转基因烟草中PVY-C的病毒积累和致病,而且对异源病毒PVX具有同样的作用,而转反义HC-Pro基因烟草对PVY-C和PVX的致病性无影响,因此,PVY-C HC-Pro基因介导PVX/PVY的协作作用。 相似文献
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39.
添加物对Bt制剂杀灭鳞翅目害虫的增效作用 总被引:2,自引:0,他引:2
以菜青虫幼虫为试虫,经室内毒力测定Bt制剂添加物的增效作用,结果表明11种添加物加入Bt制剂后均有不同的增效作用,将增效添加剂加入Bt8010菌粉,置于54摄氏度恒温条件下贮放14d后,取出测定添加物对Bt8010菌的影响,结果有6种添加物,能明显降低温贮存后Bt8010菌的芽胞数与杀虫率,田间试验结果表明,5种对Bt8010菌安全的增效添加物,按不同比例配成3组复合添加剂,再与Bt8010WP混合后,能明显提高对小菜蛾,菜青虫,甘蓝夜蛾等鳞翅目害虫的杀灭活性。 相似文献
40.
Young Lawrence J. T. Cardiff Robert D. Seeley Thomas 《In vitro cellular & developmental biology. Plant》1978,14(11):895-902
Summary Dome populations from primary cultures of mouse mammary tumor cells were quantitatively studied in regard to size, distribution,
density and the area occupied by light-diffusion photography and image analysis. The effects of fetal bovine serum, insulin
and hydrocortisone were analyzed. Quantitative characterization documented dome diameter (mode diameter 0.26 to 0.52 mm),
dome area occupied (average 23%, maximum 38.7%), and density (average 78 domes per cm2, maximum 117 domes per cm2) for standard culture conditions. Insulin and hydrocortisone had a primary effect on dome density whereas 15% fetal bovine
serum had a minimal effect. However, insulin and hydrocortisone had a synergistic optimal effect on dome population. Time-lapse
cinematography revealed that the dome population is not static, but a very dynamic one. Domes underwent irregular pulsations
of expansion and contraction. Dome enlargement was either by a series of expansions and contractions, by lateral involvement
of other cells, or by coalescence of two or more domes. Domes have been considered to be the in vitro counterpart of the in
vivo acinus of the mouse mammary gland. However, quantitative dome population characterization has not been available. Dome
analysis by light-diffraction photography and image analysis lends itself towards correlative studies of domes and their differentiative
products.
Supported in part by Public Health Service Contract NO1-CP 61013 within the Virus Cancer Program of the National Cancer Institute
and by Public Health Service Training Grant CA05245 from the National Cancer Institute. 相似文献