Leaf hydrocarbons of Phacelia species (hydrophyllaceae)

Whole leaf hydrocarbons of six species of Phacelia were investigated for their variability among natural Californian populations, and for adaptive     

Mechanism of depsipeptide formation catalyzed by enniatin synthetase

Covalently bound intermediates of enniatin B synthesis could be isolated from enniatin synthetase by treatment with performic acid. By comparison with products of mild alkaline cleavage of authentic enniatin B they could be identified as the dipeptide D-2-hydroxyisovaleryl-N-methylvaline and the corresponding tetrapeptide. Synthesis of enniatins apparently proceeds via condensation of dipeptides. This was confirmed by the use of the substrate analogue isovaleric acid, which has shown to be a strong inhibitor for enniatin synthesis by formation of N-isovaleryl-N-methyl valine.     

Catalase-like crystals in parathyroid gland cells of Rana temporaria L.

Summary Crystalline inclusions in parathyroid gland cell nuclei of Rana temporaria were studied by electron microscopy using a specimen tilting stage. Images were analysed by optical diffraction. Results were compared with X-ray and electron microscopic data of trigonal bovine liver catalase to which a striking resemblance of the inclusions was found.We are grateful to Professor R. Mosebach (Giessen) for discussions, to the Deutsche Forschungsgemeinschaft for a grant (La 229/4) and instruments and to Messrs. Spindler & Hoyer, Göttingen and Messrs. Rank Precision Instruments, Nürnberg for putting apparatus at our disposal and performing diffraction photographs.     

Zytologische Beobachtungen an der hyperplastischen Nebennierenrinde des Menschen

Summary Clinical observations and the light and electron microscopic appearance of the adrenal cortex are described in a case of Cushing's disease associated with adrenocortical hyperplasia and a basophilic adenoma of the pituitary gland. The electron microscopic investigation concentrates upon the occurrence of osmiophil material in the different cytoplasmic compartments of the cell type rich in organelles and relatively poor in paraplasmic inclusions. It seems that the observation of osmiophil material in the perinuclear space, the mitochondrial matrix, Golgi vacuoles, and cytoplasmic vesicles has been made possible by a rather long fixation in Wohlfarth-Bottermann's chromium-osmium fixative. Mitochondria and related particles (named X-Einschlüsse) may contain a light center with a few dense filaments and masses resembling those described by other authors as being of deoxyribonucleic acid nature. Relatively large Golgi complexes are found, sometimes in close topographic relation with a centriole. A large population of osmiophil bodies (lipid droplets, pigment granules, microbodies) is described. Apart from these findings the cytoplasmic organization in the tissue studied is similar to that of the adrenal cortex as described in other mammals. The observations are discussed with respect to hypotheses about lipid droplet formation and to the possibility of artifacts due to preparation procedures.

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Herrn Prof. Dr. med. W. Bargmann zum 60. Geburtstag gewidmet. — Herrn Prof. Dr. E. Uehlinger, Direktor des Pathologischen Instituts der Universität Zürich, danken wir für die Überlassung des Materials und für die freundlicherweise zur Verfügung gestellte pathologisch-anatomische Diagnose, dem Schweizerischen Nationalfonds für die Unterstützung und Frau Jäger, Mlle. Challamel und Herrn Krieger für die Ausführung der histologischen und photographischen Arbeiten.     

Augmentation of host antitumor immunity by low doses of cyclophosphamide and mafosfamide in two animal tumor models

Summary By cloning in vitro we have obtained two sublines of the L5222 rat leukemia, one with high (L5222-S) and the other with low (L5222-R) in vivo sensitivities to non-toxic doses of mafosfamide, a stabilized derivative of 4-hydroxy-cyclophosphamide. This sensitivity in vivo was not related to the cytotoxic activity of the drug in vitro. Treatment of rats bearing the L5222-S and of mice transplanted with the MOPC-315 plasmocytoma with low doses of mafosfamide or cyclophosphamide resulted in a high percentage of surviving animals, which were resistant to a subsequent tumor challenge. Viable leukemic cells were needed to establish antitumor immunity, since it was not possible to induce resistance by injection of mitomycin-C-treated, non-viable L5222 cells. The adoptive transfer of spleen cells from animals immune against the L5222-S and the MOPC-315 resulted in resistance of the syngeneic recipients against a rechallenge with tumor cells, provided that the animals were treated with an immunosuppressive dose (100 mg/kg) of cyclophosphamide prior to the spleen cell implantation. In nude mice treatment of the L5222 with low doses of mafosfamide also resulted in surviving animals, however resistance to a second tumor challenge occurred only sporadically.The data presented confirm that therapy with cyclophosphamide or mafosfamide enhances host antitumor immunity but, contrary to previous reports, it could be demonstrated that successful tumor rejection was independent of T cells.Supported by the Federal Ministry of Research and Technology (BMFT), Bonn-Bad Godesberg, FRG     

Community composition and soil properties in northern Bolivian savanna vegetation

Abstract. Direct gradient analysis (Canonical Correspondence Analysis) of northern Bolivian savanna vegetation revealed correlations between the composition of plant communities and physical and chemical soil properties. Cover/abundance values for 193 species from 27 sites were related to data on eight soil factors. A water-regime variable and the percentage of sand and silt were correlated with the first axis of the species-environment biplot and explained most of the variation in community composition. Along this axis, species and sites of flood-plain vegetation were separated from sites not affected by flooding rivers. The second axis of the biplot was correlated with soil-chemical variables, namely extract-able phosphate, base saturation, organic carbon, pH, and effective cation exchange capacity. Part of the variation in community composition can be explained by these soil nutrient variables. Grassland communities were separated from woody vegetation along the soil nutrient gradient, and floodplain communities of white-water rivers from those of clear-water rivers. The results of the gradient analysis indicate that the soil texture-moisture gradient is the prime factor determining the variation in the floristic composition of the savanna communities examined, and that, in addition, the soil nutrient gradient accounts for some of the variation.     

Long term study on the influence of eutrophication, restoration and biomanipulation on the structure and development of phytoplankton communities in Feldberger Haussee (Baltic Lake District, Germany)

Feldberger Haussee provides a classic example of eutrophication history of hardwater lakes in the Baltic Lake District (Germany) and of changes in their algal flora during the 20th century. The lake originally was regarded as slightly eutrophic. A process of drastic eutrophication from the 1950s until the end of the 1970s caused mass developments of blue-green and green algae. A restoration program was started in the 1980s to improve the water quality of the lake using both diversion of sewage outside the catchment area, and biomanipulation by altering the fish community. This restoration program led to positive changes in the lake ecosystem. Direct effects of biomanipulation resulted in an increase of herbivorous zooplankton, a decrease of phytoplankton biomass, and an increase of water transparency. The recovery of Feldberger Haussee also may have been indirectly enhanced by an increase in nutrient sedimentation as a consequence of intensified calcite precipitation, decrease in phosphorus remobilization due to a pH-decrease, increased NIP-ratio, and recolonization of the littoral zone by macrophytes. This paper concentrates on the long term development of the phytoplankton community as a response to changes in the food web structure as well as to alterations in the chemical environment of the algae. Both are reflected in four major stages passed by the algal assemblage between 1980 and 1994: (1) From 1980-summer 1985 dense green algal populations were found indicating similar conditions as in the 1970s during the period of maximum eutrophication. (2) A diverse phytoplankton community during summer 1985–1989 showed the first effects of a recovery. (3) From 1990–1992 the phytoplankton was characterized by ungrazeable filamentous blue-green algae first of all as a response to increased herbivory of zooplankton on edible species and to increasing N/P-ratios. (4) Finally, the algal species diversity increased in 1993 and 1994 whereas the phytoplankton biomass decreased showing the success of the combined restoration measures.     

HSP25 in isolated perfused rat hearts: Localization and response to hyperthermia

Recent investigations concentrate on the correlation between the myocardial expression of the inducible 70-kDa heat shock protein (HSP70i) by different stress conditions and its possible protective effects. Only few studies have focused on the involvement of small heat shock proteins in this process. We analyzed the location of the small heat shock protein HSP25 in isolated cardiomyocytes as well as its location and induction in isolated perfused hearts of rats. By immunofluorescence microscopy HSP25 was found to colocalize with actin in the I-band of myofibrils in cardiomyocytes of isolated perfused hearts as well as in isolated neonatal and adult cardiomyocytes. Hyperthermic perfusion of isolated hearts for 45 min resulted in modulation of different parameters of heart function and in induction of HSP25 and HSP70i. Temperatures higher than 43°C (44–46°C) were lethal with respect to the contractile function of the hearts. Compared to control hearts perfused at 37°C, significant increases during hyperthermic perfusion at 42°C and 43°C were obtained for heart rate, contraction velocity and relaxation velocity. In response to hyperthermia at 43°C and after subsequent normothermic perfusion for 135 min at 37°C, left ventricular pressure, contraction velocity and relaxation velocity remained significantly elevated. However, heart rate returned to control values immediately after the period of heat treatment. HSP25 is constitutively expressed even in normothermic perfused hearts as shown by Western blotting. Hyperthermia increased the content of HSP25 only in the left ventricular tissue. In contrast, HSP70i was strongly induced in all analyzed parts of the myocardium (left ventricle, right ventricle, septum). Our findings suggest a differential regulation of HSP25 and HSP70i expression in response to hyperthermia in isolated perfused hearts. The constitutively expressed HSP25 seems to be located adjacent to the myofibrils which implies a specific role of this protein even under unstressed conditions for the contractile function of the myocardium.