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排序方式: 共有265条查询结果,搜索用时 609 毫秒
31.
Samadi A Soriano E Revuelta J Valderas C Chioua M Garrido I Bartolomé B Tomassolli I Ismaili L González-Lafuente L Villarroya M García AG Oset-Gasque MJ Marco-Contelles J 《Bioorganic & medicinal chemistry》2011,19(2):951-960
The synthesis, structure, theoretical and experimental in vitro antioxidant properties using the DPPH, ORAC, and benzoic acid, as well as preliminary in vitro pharmacological activities of (Z)-??-aryl and heteroaryl N-alkyl-nitrones 6-15, 18, 19, 21, and 23, is reported. In the in vitro antioxidant activity, for the DPPH radical test, only nitrones bearing free phenol groups gave the best RSA (%) values, nitrones 13 and 14 showing the highest values in this assay. In the ORAC analysis, the most potent radical scavenger was nitrone indole 21, followed by the N-benzyl benzene-type nitrones 10 and 15. Interestingly enough, the archetypal nitrone 7 (PBN) gave a low RSA value (1.4%) in the DPPH test, or was inactive in the ORAC assay. Concerning the ability to scavenge the hydroxyl radical, all the nitrones studied proved active in this experiment, showing high values in the 94-97% range, the most potent being nitrone 14. The theoretical calculations for the prediction of the antioxidant power, and the potential of ionization confirm that nitrones 9 and 10 are among the best compounds in electron transfer processes, a result that is also in good agreement with the experimental values in the DPPH assay. The calculated energy values for the reaction of ROS (hydroxyl, peroxyl) with the nitrones predict that the most favourable adduct-spin will take place between nitrones 9, 10, and 21, a fact that would be in agreement with their experimentally observed scavenger ability. The in vitro pharmacological analysis showed that the neuroprotective profile of the target molecules was in general low, with values ranging from 0% to 18.7%, in human neuroblastoma cells stressed with a mixture of rotenone/oligomycin-A, being nitrones 18, and 6-8 the most potent, as they show values in the range 24-18.4%. 相似文献
32.
HPLC coupled on-line to ESI-MS and a DPPH-based assay for the rapid identification of anti-oxidants in Butea superba 总被引:1,自引:0,他引:1
Nuengchamnong N de Jong CF Bruyneel B Niessen WM Irth H Ingkaninan K 《Phytochemical analysis : PCA》2005,16(6):422-428
A reversed-phase HPLC coupled on-line to a radical scavenging detection system and MS/MS was developed in order to combine separation, activity determination and structural identification of anti-oxidants in complex mixtures in one run. The sample was separated by HPLC and the eluate split into two flows. The major portion was fed into an electrospray ionisation MS/MS system, while the minor part was mixed with a free radical, 2,2'-diphenyl-1-picrylhydrazyl (DPPH), and the reaction determined spectrophotometrically. The negative peaks, which indicated the presence of anti-oxidant activity, were monitored by measuring the decrease in absorbance at 517 nm. The developed method was successfully applied to the identification of anti-oxidant compounds in a fraction, obtained by solid-phase extraction, of an extract of a Thai medicinal plant, Butea superba Roxb. The anti-oxidant compounds were separated and identified as procyanidin B2, (-)-epicatechin and procyanidin B5. 相似文献
33.
Kang KA Lee KH Chae S Zhang R Jung MS Lee Y Kim SY Kim HS Joo HG Park JW Ham YM Lee NH Hyun JW 《FEBS letters》2005,579(28):6295-6304
We have investigated the cytoprotective effect of eckol, which was isolated from Ecklonia cava, against oxidative stress induced cell damage in Chinese hamster lung fibroblast (V79-4) cells. Eckol was found to scavenge 1,1-diphenyl-2-picrylhydrazyl radical, hydrogen peroxide (H(2)O(2)), hydroxy radical, intracellular reactive oxygen species (ROS), and thus prevented lipid peroxidation. As a result, eckol reduced H(2)O(2) induced cell death in V79-4 cells. In addition, eckol inhibited cell damage induced by serum starvation and radiation by scavenging ROS. Eckol was found to increase the activity of catalase and its protein expression. Further, molecular mechanistic study revealed that eckol increased phosphorylation of extracellular signal-regulated kinase and activity of nuclear factor kappa B. Taken together, the results suggest that eckol protects V79-4 cells against oxidative damage by enhancing the cellular antioxidant activity and modulating cellular signal pathway. 相似文献
34.
New phenolic constituents from Balanophora polyandra with radical-scavenging activity 总被引:1,自引:0,他引:1
The 80% acetone extract of Balanophora polyandra Griff. (Balanophoraceae) was found to exhibit high radical-scavenging activity (SC(50)=14.48 mug/ml) towards 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Further chemical investigation led the isolation of two new hydrolysable tannins, balapolyphorins A (1) and B (2), together with 20 known phenolic compounds (3-22). Their structures were established by detailed spectroscopic analysis, and the radical-scavenging properties of all isolated compounds were determined by DPPH assay. 相似文献
35.
酶辅助提取莲房原花青素工艺及其抗氧化活性研究 总被引:3,自引:0,他引:3
主要用纤维素酶和果胶酶对莲房组织进行酶解,以提高莲房原花青素提取率。采用四因素三水平正交实验对酶解时间、加酶量和酶解温度等提取工艺参数进行优化,获得了最佳工艺参数为纤维素酶添加量为0.7%,果胶酶添加量0.1%,酶解温度55℃,酶解时间2.5h,优化后的提取工艺与直接醇提法相比,能将莲房原花青素的提取率提高约48%。在此基础上采用DPPH法进行了抗氧化性的对比,结果表明酶辅助提取和醇提法提取的原花青素有着相同的抗氧化性。 相似文献
36.
Delazar A Biglari F Esnaashari S Nazemiyeh H Talebpour AH Nahar L Sarker SD 《Phytochemistry》2006,67(19):2176-2181
A combination of vacuum liquid chromatography (VLC) and preparative thin layer chromatography (PTLC) of the dichloromethane extract of the aerial parts of the Iranian plant Pimpinella aurea afforded two phenylpropanoids, erythro-1'-(4-methoxyphenyl)-propan-1',2'-diol (1) and erythro-1'-[4-(sec-butyl)-phenyl]-propan-1',2'-diol (2), the latter being a natural product. The structures of these compounds were determined by spectroscopic means. The antioxidant properties of these compounds were assessed by the DPPH assay. The GC-MS analysis of the essential oils of P. aurea provided a chemical profile that was significantly different from the previously published reports. 相似文献
37.
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39.
芦丁-锗配合物及其自由基清除活性研究 总被引:8,自引:0,他引:8
芦丁是存在于多种植物中的天然多羟基黄酮苷,能与多种金属离子形成配合物。本文采用紫外分光度法考察了芦丁与锗离子的配位作用,并研究了芦丁-锗配合物清除超氧自由基和DPPH自由基的作用。结果显示在KH2PO4-NaOH(pH6.70)的缓冲液中,芦丁与锗离子能形成1:1的配合物,其K稳=10^7.46,同时配合物显示有较好的自由基清除活性。 相似文献
40.
《Free radical research》2013,47(4):257-267
AbstractIn the current study, we analysed free radicals scavenging activity of monocytes-macrophages in the absence or presence of antioxidants such as polyphenols or paraoxonase 1 (PON1). THP-1 human monocytic cell line, murine J774A.1 macrophages, as well as human primary monocytes have the capability to scavenge free radicals, as measured by the 1-diphenyl-2-picryl-hydrazyl (DPPH) assay. This effect (which could be attributed to the cell's membrane) was cell number and incubation time dependent. Upon incubation of J774A.1 macrophages with acetylated LDL (Ac-LDL), with VLDL, or with the radical generator, AAPH, the cells’ lipid peroxides content, and paraoxonase 2 (PON2) activity were significantly increased. While non-treated cells decreased DPPH absorbance by 65%, the Ac-LDL-, VLDL- or AAPH-treated cells, decreased it by only 33%, 30%, or 45%, respectively. We next analysed the effect of J774A.1 macrophage enrichment with antioxidants, such as polyphenols or PON1 on the cells’ free radicals scavenging activity. Non-treated cells decreased DPPH absorbance by 50%, whereas vitamin E-, punicalagin- or PJ-treated cells significantly further decreased it, by 75%. Similarly, in PON1-treated cells DPPH absorbance was further decreased by 63%, in association with 23% increment in PON1 catalytic activity. In cells under oxidative stress [treated with AAPH-, or with oxidized LDL], PON1 activity was decreased by 31% or 40%, as compared to the activity observed in PON1 incubated with non-treated cells. We conclude that monocytes-macrophages possess free radicals scavenging activity, which is decreased under atherogenic conditions, and increased upon cell enrichment with potent antioxidants such as nutritional polyphenols, or PON1. 相似文献