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21.
Autofluorescence in eleocytes of some earthworm species 总被引:2,自引:0,他引:2
Cholewa J Feeney GP O'Reilly M Stürzenbaum SR Morgan AJ Płytycz B 《Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society》2006,44(1):65-71
Immunocompetent cells of earthworms, coelomocytes, comprise adherent amoebocytes and granular eleocytes (chloragocytes). Both cell populations can be expelled via dorsal pores of adult earthworms by exposure to an electric current (4.5 V) for 1 min. Analysis by phase contrast/fluorescence microscopy and flow cytometry demonstrated that eleocyte population of several species exhibits a strong autofluorescence. A high percentage (11-35%) of autofluorescent eleocytes was recorded in Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion sp. (O. cyaneum, O. tyrtaeum tyrtaeum and O. tyrtaeum lacteum). In contrast, autofluorescent coelomocytes were exceptionally scarce (less than 1%) in representative Aporrectodea sp. (A. caliginosa and A. longa) and Lumbricus sp. (L. castaneus, L. festivus, L. rubellus, L. terrestris). Thus, this paper for the first time describes profound intrinsic fluorescence of eleocytes in some--but not all--earthworm species. The function (if any) and inter-species differences of the autofluorescent coelomocytes still remain elusive. 相似文献
22.
Qi Guo Jasmin K. Sidhu Timothy M. D. Ebbels Faisal Rana David J. Spurgeon Claus Svendsen Stephen R. Stürzenbaum Peter Kille A. John Morgan Jacob G. Bundy 《Metabolomics : Official journal of the Metabolomic Society》2009,5(1):72-83
One of the promises of environmental metabolomics, together with other ecotoxicogenomic approaches, is that it can give information
on toxic compound mechanism of action (MOA), by providing a specific response profile or fingerprint. This could then be used
either for screening in the context of chemical risk assessment, or potentially in contaminated site assessment for determining
what compound classes were causing a toxic effect. However for either of these two ends to be achievable, it is first necessary
to know if different compounds do indeed elicit specific and distinct metabolic profile responses. Such a comparative study
has not yet been carried out for the earthworm Lumbricus rubellus. We exposed L. rubellus to sub-lethal concentrations of three very different toxicants (CdCl2, atrazine, and fluoranthene, representing three compound classes with different expected MOA), by semi-chronic exposures
in a laboratory test, and used NMR spectroscopy to obtain metabolic profiles. We were able to use simple multivariate pattern-recognition
analyses to distinguish different compounds to some degree. In addition, following the ranking of individual spectral bins
according to their mutual information with compound concentrations, it was possible to identify both general and specific
metabolite responses to different toxic compounds, and to relate these to concentration levels causing reproductive effects
in the worms.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Qi Guo and Jasmin K. Sidhu contributed equally to this paper. 相似文献
23.
Liisa M. Hirvonen Jakub Nedbal Norah Almutairi Thomas A. Phillips Wolfgang Becker Thomas Conneely James Milnes Susan Cox Stephen Stürzenbaum Klaus Suhling 《Journal of biophotonics》2020,13(2)
We report on wide‐field time‐correlated single photon counting (TCSPC)‐based fluorescence lifetime imaging microscopy (FLIM) with lightsheet illumination. A pulsed diode laser is used for excitation, and a crossed delay line anode image intensifier, effectively a single‐photon sensitive camera, is used to record the position and arrival time of the photons with picosecond time resolution, combining low illumination intensity of microwatts with wide‐field data collection. We pair this detector with the lightsheet illumination technique, and apply it to 3D FLIM imaging of dye gradients in human cancer cell spheroids, and C. elegans. 相似文献
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