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排序方式: 共有180条查询结果,搜索用时 15 毫秒
171.
Silke Walter Axinia Doering Maryse Letiembre Yang Liu Wenlin Hao Ricarda Diem Christian Bernreuther Markus Glatzel Britta Engelhardt Klaus Fassbender 《Cellular physiology and biochemistry》2006,17(3-4):167-172
Innate immune receptors are crucial for defense against microorganisms. Recently, a cross-talk between innate and adaptive immunity has been considered. Here, we provide first evidence for a role of the key innate immune receptor, LPS receptor (CD14) in pathophysiology of experimental autoimmune encephalomyelitis, the animal model of multiple sclerosis. Indicating a functional importance in vivo, we show that CD14 deficiency increased clinical symptoms in active experimental autoimmune encephalomyelitis. Consistent with these observations, CD14 deficient mice exhibited a markedly enhanced infiltration of monocytes and neutrophils in brain and spinal cord. Moreover, we observed an increased immunoreactivity of CD14 in biopsy and post mortem brain tissues of multiple sclerosis patients compared to age-matched controls. Thus, the key innate immune receptor, CD14, may be of pathophysiological relevance in experimental autoimmune encephalomyelitis and multiple sclerosis. 相似文献
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Ngo Thuy Diem Trang Dennis Konnerup Hans-Henrik Schierup Nguyen Huu Chiem Le Anh Tuan Hans Brix 《Ecological Engineering》2010,36(4):527-535
The treatment capacity of constructed wetlands is expected to be high in tropical areas because of the warm temperatures and the associated higher rates of microbial activity. A pilot scale horizontal subsurface flow constructed wetland system filled with river sand and planted with Phragmites vallatoria (L.) Veldkamp was set up in the southern part of Vietnam to assess the treatment capacity and the removal rate kinetics under tropical conditions. The system received municipal wastewater at four hydraulic loading rates (HLRs) of 31, 62, 104 and 146 mm day?1. Removals of TSS, BOD5 and COD were efficient at all HLRs with mean removal rates of 86–95%, 65–83% and 57–84%, respectively. Removals of N and P decreased with HLRs and were: NH4-N 0–91%; TN 16–84% and TP 72–99%. First-order area-based removal rate constants (k, m year?1) estimated from sampling along the length of the wetland from inlet to outlet at the four HLRs were in the range of 25–95 (BOD5), 22–30 (COD), 31–115 (TSS), 5–24 (TN and TKN) and 41–84 (TP) at background concentrations (C*) of 5, 10, 0, 1.5 and 0 mg L?1, respectively. The estimated k-values should not be used for design purposes, as site-specific differences and stochastic variability can be high. However, the study shows that domestic wastewater can be treated in horizontal subsurface flow constructed wetland systems to meet even the most stringent Vietnamese standards for discharge into surface waters. 相似文献
174.
Résumé La méthode d'infection des plantules de tomate est appliquée pour classer, selon le potentiel d'infection d? àP. lycopersici, les différents sols de serre ayant porté de fa?on plus ou moins régulières, des cultures de tomate. 相似文献
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Susie Boydston-White Melissa Romeo Tatyana Chernenko Miloš Miljkovi? Max Diem 《生物化学与生物物理学报:生物膜》2006,1758(7):908-914
We have previously reported spectral differences for cells at different stages of the eukaryotic cell division cycle. These differences are due to the drastic biochemical and morphological changes that occur as a consequence of cell proliferation. We correlate these changes in FTIR absorption and Raman spectra of individual cells with their biochemical age (or phase in the cell cycle), determined by immunohistochemical staining to detect the appearance (and subsequent disappearance) of cell-cycle-specific cyclins, and/or the occurrence of DNA synthesis. Once spectra were correlated with their cells' staining patterns, we used methods of multivariate statistics to analyze the changes in cellular spectra as a function of cell cycle phase. 相似文献
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Multispecific antibody formats provide a promising platform for the development of novel therapeutic concepts that could facilitate the generation of safer, more effective pharmaceuticals. However, the production and use of such antibody-based multispecifics is often made complicated by: 1) the instability of the antibody fragments of which they consist, 2) undesired inter-subunit associations, and 3) the need to include recombinant heterodimerization domains that confer distribution-impairing bulk or enhance immunogenicity. In this paper, we describe a broadly-applicable method for the stabilization of human or humanized antibody Fv fragments that entails replacing framework region IV of a Vκ1/VH3-consensus Fv framework with the corresponding germ-line sequence of a λ-type VL chain. We then used this stable Fv framework to generate a novel heterodimeric multispecific antibody format that assembles by cognate VL/VH associations between 2 split variable domains in the core of the complex. This format, termed multispecific antibody-based therapeutics by cognate heterodimerization (MATCH), can be applied to produce homogeneous and highly stable antibody-derived molecules that simultaneously bind 4 distinct antigens. The heterodimeric design of the MATCH format allows efficient in-format screening of binding domain combinations that result in maximal cooperative activity. 相似文献
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