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Effects of peach branches returning on autotoxins and microbes in soil and tree growth of peaches 下载免费PDF全文
《植物生态学报》2016,40(2):140
Aims This study aimed to investigate the effects of branch returning on the growth of peach (Amygdalus persica "Chunmei/Maotao") saplings, soil enzyme activity, and soil contents of phenolic acids and amygdalin, thereby providing scientific evidence against the application of branch returning for peach trees. Methods One-year-old potted peach tree (Amygdalus persica "Chunmei/Maotao") was used in this study with four agricultural treatments applied, including soil coverage by fragmented peach tree branches (fragment treatment;1.5 and 22.5 g·kg-1) and applying leachate solutions of peach tree branches to soil (leachate treatment; 1.5 and 22.5 g·kg-1). No branch addition was used as control (CK). Solid phase extraction, high performance liquid chromatography (HPLC), biological high-throughput sequencing was used to determine the content of autotoxic substances, and microbial community structure in soil. Soil coverage and leachate solution treatments of 30 g and 450 g branches applied to the peach trees were described as 1.5 and 22.5 g·kg-1, respectively in this paper.Important findings Compared with CK, the phenolic acid and amygdalin contents significantly increased after both fragment and leachate treatments in high quantities (22.5 g·kg-1). Soil microbial community structure altered in both treatments, with the proportion of fungi (particularly Agaricomycetes, Tubeufia and Cystofilobasidiaceae) increased significantly and bacteria decreased accordingly. Invertase activity in both high-quantity treatments exceeded that in the CK significantly. The activity of catalase and urease was higher at first and then decreased relative to CK under high-quantity fragment and leachate treatments. Specifically, the effect of leachate treatment on enzyme activity was higher than the fragment treatment in the short term. Chlorophyll content, ground diameter (diameter of 5 cm from the ground) growth and net photosynthesis rate of plants were lower in high-quantity fragment and leachate treatments than those in CK, with earlier retardation of new shoot growth. We observed an increase in soil phenolic acids and enzymes in treatments in normal pruning quantity, while no inhibition effect was found on the tree growth. In conclusion, autotoxins (such as phenolic acid and amygdalin) inhibited the growth of peach trees both directly and indirectly through changing soil enzyme activity and microbial community. 相似文献
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SUMO化是一种重要的蛋白质翻译后修饰,对植物正常生长发育不可或缺。到目前为止已筛选到上千个可能的SUMO底物,但由于SUMO化修饰水平普遍很低,其生物学功能研究相对较少。该文详细描述了检测蛋白SUMO化修饰的常用方法,包括体外和体内SUMO化实验,以及SUMO化修饰位点的检测方法,旨在为深入研究植物蛋白SUMO化修饰提供技术支持。 相似文献
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火炬松(Pinus taeda)又名台大松,原产美国南部,在原产地树高可达30米左右。该树种喜温暖湿润的气候,能耐干燥瘠薄的土壤;具有很强的抗松毛虫和松干蚧的特性,对海风和海雾也有较强的抗性。我国安徽、江苏、浙江、福建、广东和江西等省均已广泛引种。火炬松一般在3月下旬开始 相似文献
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物理方法在提高植物抗逆性中的应用 总被引:2,自引:0,他引:2
介绍了物理因素处理,主要包括磁场、电场、热激、冷激与低温以及激光处理等提高植物抗逆性的研究进展. 相似文献