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排序方式: 共有389条查询结果,搜索用时 62 毫秒
101.
Abstract Industrial activities discharge a large amount of wastes containing hexavalent chromium [Cr(VI)] into the environment, which poses a threat to human health. Microorganisms can be used as efficient tools for Cr(VI) remediation. In this study, the Cr(VI) removal capacity of Aspergillus niger was evaluated. A. niger could tolerate and reduce Cr(VI) by nearly 100% at concentrations ranging from 10 to 50?mg/L. Overall, almost 97% of the Cr(VI) removal was caused by extracellular reduction whereas 3% was caused by accumulation. Extracellular reduction was mediated by non-enzymatic cell secretions, whereas extracellular accumulated Cr formed precipitates on the hyphal surfaces and was partially absorbed on the cell wall. Cr(VI) also entered the cell and was reduced by the strong chromate reductase activity in cell-free extracts and then accumulated within the cell. These data suggest that A. niger, which has the capacity to remove Cr(VI) by reduction and accumulation, can be a useful tool for Cr(VI) remediation. 相似文献
102.
【背景】六价铬[Cr(VI)]作为一种高毒性的重金属污染物,用微生物学方法还原Cr(VI)既经济又环保。【目的】探究耐铬菌株CM01中与耐受或还原铬Cr(VI)有关的蛋白或基因,从分子水平上阐述其耐铬Cr(VI)机制。【方法】运用同位素标记相对和绝对定量技术(isobarictagsforrelativeand absolute quantitation techniques,iTRAQ)分析耐铬菌株CM01在有无铬Cr(VI)胁迫下的蛋白表达差异。运用RT-qPCR技术验证4种与代谢途径有关的上调蛋白的m RNA表达量。运用紫外分光光度计测定细胞表面疏水性。【结果】共鉴定到2 570个蛋白,其中存在显著差异的蛋白数为646个,上调蛋白数量343个,下调蛋白数量303个。CM01中的Fe/Mn超氧化物歧化酶、甜菜碱醛脱氢酶、磷酸戊糖途径、磷酸肌醇代谢途径、氨基酸代谢共同参与了菌株对于外源性Cr(VI)的响应机制。RT-qPCR实验结果表明,与代谢途径有关的4种蛋白其基因转录水平和蛋白水平一致。对照组CM01的细胞表面疏水性高于实验组。【结论】初步探索了耐铬菌的Cr(VI)响应机制,为利用微生物治理环境污染提供了理论支持和新的研究思路。 相似文献
103.
Li Chen T LaCerte C Wise SS Holmes A Martino J Wise JP Thompson WD Wise JP 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2012,155(1):143-150
Chromium (Cr) is a global marine pollutant, present in marine mammal tissues. Hexavalent chromium [Cr(VI)] is a known human carcinogen. In this study, we compare the cytotoxic and clastogenic effects of Cr(VI) in human (Homo sapiens) and sperm whale (Physeter macrocephalus) skin fibroblasts. Our data show that increasing concentrations of both particulate and soluble Cr(VI) induce increasing amounts of cytotoxicity and clastogenicity in human and sperm whale skin cells. Furthermore, the data show that sperm whale cells are resistant to these effects exhibiting less cytotoxicity and genotoxicity than the human cells. Differences in Cr uptake accounted for some but not all of the differences in particulate and soluble Cr(VI) genotoxicity, although it did explain the differences in particulate Cr(VI) cytotoxicity. Altogether, the data indicate that Cr(VI) is a genotoxic threat to whales, but also suggest that whales have evolved cellular mechanisms to protect them against the genotoxicity of environmental agents such as Cr(VI). 相似文献
104.
Meibian Zhang Zhijian Chen Qing Chen Hua Zou Jianlin Lou Jiliang He 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2008,654(1):45-51
DNA damage of peripheral lymphocytes in 60 workers occupationally exposed to trivalent chromium [Cr(III)] in a tannery was studied using comet assay. The urinary and blood chromium levels were detected as a biomarker of internal exposure. The 90 subjects were divided into three groups: (i) exposure group I included 30 tannery workers highly exposed to chromium from tanning department; (ii) exposure group II included 30 tannery workers with moderate chromium exposure from finishing department; (iii) control group included 30 individuals without exposure to physical or chemical genotoxic agents. No significant difference was found among the three groups for age and smoking. The results showed that the medians of blood and urinary Cr of two exposure groups were significantly higher than those of control group (P < 0.01). And the medians of blood and urinary Cr of exposure group I were significantly higher than those of exposure group II (P < 0.05 or P < 0.01). The medians of mean tail length (MTL) of the three groups were 5.33 (2.90–8.50), 3.43 (2.31–8.29) and 2.04 (0.09–3.83) μm, respectively; The medians of mean tail moment (MTM) of the three groups were 6.28 (2.14–11.81), 3.41 (1.25–11.07) and 0.53 (0.13–3.29), respectively. The MTL and MTM of two exposure groups were significantly higher than those of control group (P < 0.01). The MTL and MTM of exposure group I were significantly higher than those of exposure group II (P < 0.01). The results of the present investigation suggest that occupational exposure to trivalent chromium can lead to a detectable DNA damage of human peripheral lymphocytes. Moreover, DNA damage was associated with chromium levels in blood. DNA damage may serve as a valuable effective biomarker and total chromium in blood may serve as a useful internal exposure biomarker in the population occupationally exposed to trivalent chromium. 相似文献
105.
多肽铬螯合物对糖尿病小鼠肝脏蛋白质表达的影响 总被引:1,自引:1,他引:0
目的:研究多肽铬螯合的对糖尿病小鼠肝脏蛋白质表达的影响,探讨其治疗糖尿病的机理.方法:通过腹腔注射四氧嘧啶建立糖尿病小鼠模型.将小鼠分为正常组、模型组和胶铬组,胶铬组以灌胃方式加入多肽铬螯合物;以光镜及HE染色观察三组小鼠肝脏形态及组织学的变化,采用SDS-PAGE实验和非SDS-PAGE检测三组小鼠肝脏蛋白质表达.结果:光镜及组织学观察结果显示多肽铬螫合物可以有效地减轻四氧嘧啶对肝细胞造成的损伤.模型组肝脏25kDa-35kDa之间的某种蛋白表达升高而多肽铬螯舍物可以降低此种蛋白的表达,初步推断这种蛋白质为SOD.结论:多肽铬螯合物能够通过降低四氧嘧啶造成的肝脏中抗氧化有关的蛋白质代偿性升高而祈祷保护肝脏的作用,是一种新型的治疗糖尿病所致的肝损伤的活性物质. 相似文献
106.
Selection of a high-biomass, chromium-rich yeast strain and optimization of cultivation conditions 总被引:3,自引:0,他引:3
J Liu B Zhang X He P Zhang Z Chai 《Journal of industrial microbiology & biotechnology》2001,27(4):195-198
Saccharomyces cerevisiae LZ-53 was selected from 240 primary yeast strains from different genera and species, whose chromium (Cr) resistance and biomass
were higher than others were. The highest biomass and Cr content of the strain was obtained in 30 h at 28°C and 200 rpm, when
20 ml of the culture in 250-ml shake flasks was grown in wort containing 1200 μg/ml Cr. The initial pH was adjusted to 6.0.
The optimal inoculum volume was 10% (v/v). The Cr content of the cells was determined by neutron activation analysis. Under
the optimized cultivation conditions, the Cr content reached 3248 μg/g. Journal of Industrial Microbiology & Biotechnology (2001) 27, 195–198.
Received 28 January 2001/ Accepted in revised form 11 June 2001 相似文献
107.
Summary Summerford Bing is an old industrial site near Falkirk which was contaminated from about1860–1970 by chemical and other works. The industrial wastes were redistributed in a spoil heap about 300 m × 300 m in area. Drainage water from the heap was known to have high concentrations of chromium. An attempt to establish birch on the spoil heap's slopes has been partially successful but many of the trees were very stunted. Soil analyses showed that hexavalent chromium toxicity was the most likely cause of the poor birch growth and this was confirmed by an experiment on birch seedlings. Acute plant chromium toxicity is a rare phenomenon in Britain where it is always associated with anthropogenic soils. The possibility of the site's toxicity to humans is discussed and it is concluded that the site is potentially hazardous to health. 相似文献
108.
《Chemical Speciation and Bioavailability》2013,25(2):31-39
AbstractChromium speciation in the presence of organic chromium(III) complexes was investigated using solid-phase extraction. The adsorptions of Cr(VI) and Cr(III) on alumina and pumice powder were studied. Maximum sorption of Cr(VI) was obtained by alumina (90.22%), while Cr(III) was highly adsorbed onto pumice powder (86.65%). This result shows that pumice may be a new and promising adsorbent for Cr(III). The experimental equilibrium data for Cr(VI) adsorption onto alumina and Cr(III) sorption onto pumice were analysed using Langmuir and Freundlich isotherms. The separation and adsorption of Cr(VI), Cr(III) and five organic chromium(III) complexes onto pumice and alumina at different pH values were evaluated. Ethylenediaminetetraacetate (EDTA), oxalate, citrate, glycine, alanine and 8-hydroxyqinoline were used as ligands. Sorption of alanine and ethylenediaminetetraacetate complexes was higher onto alumina than pumice at pH>3. The enhancement of adsorption of chromium(III) complexes onto pumice was achieved by surface modification of pumice using a surfactant, namely hexadecyltrimethylammoniumbromür (HDTMA). The presence of surfactant enhanced the adsorption of Cr(III) citrate, oxalate, glycine and 8-hydroxyquinoline complexes onto pumice. However, the adsorption of EDTA and alanine complexes decreased, with ratio of 13.40% and 4.00% respectively. Here we demonstrate that chromium speciation methods depending on adsorption onto various adsorbents including alumina may lead erroneous results. Analytical measurements were performed by flame AAS, data were obtained by standard addition method. 相似文献
109.
《Animal : an international journal of animal bioscience》2019,13(5):983-991
Dietary supplementation with the organic chromium (Cr) has been shown to positively affect the immune function of poultry. However, to our knowledge, no experiment has been done to directly compare the impacts of Cr chloride and chromium picolinate (CrPic) on the immune responses of broilers vaccinated with Avian Influenza (AI) virus vaccine. Therefore, the present experiment was conducted to investigate the effects of supplemental Cr sources (Cr chloride and CrPic) and levels on the growth performance and immune responses of broilers vaccinated with AI virus vaccine so as to provide an effective nutritional strategy for improving immune function of broilers. A total of 432 1-day (d)-old male broiler chicks were used in a 1 plus 2×4 design. Chickens were given either a diet without Cr supplementation (control) or diets supplemented with 0.4, 0.8, 1.6, or 3.2 mg Cr/kg as either Cr chloride or CrPic for 42 d. Compared to the control, dietary Cr supplementation had no effect (P>0.05) on average daily gain, average daily feed intake and gain : feed of broilers during the starter and grower phases, but increased (P<0.05) the relative weights of bursa of fabricius on d 21 and thymus, spleen, or bursa of fabricius on d 42, serum antibody titers against AI virus on d 21, 28, 35 and 42, blood T-lymphocyte transformation rate on d 28 and 42, blood T-lymphocyte percentage on d 42, and serum interleukin-2 contents on d 28. Broilers fed the diets supplemented with the inorganic Cr chloride had higher (P<0.05) weights of thymus, spleen and bursa of fabricius than those fed the diets supplemented with the CrPic on d 42. In addition, broilers fed the diets supplemented with the CrPic had higher (P<0.05) antibody titers against AI virus than those fed the diets supplemented with the inorganic Cr chloride on d 21 and 35. These results indicate that dietary Cr supplementation improved immune responses of broilers vaccinated with AI virus, and the inorganic Cr chloride was more effective than the CrPic in increasing the relative weights of lymphoid organs, however, the CrPic was more effective than the inorganic Cr chloride in enhancing the serum antibody titer against AI virus. 相似文献
110.
Hira Amin Basir Ahmed Arain Muhammad Sadiq Abbasi Farah Amin Taj Muhammad Jahangir Noor-ul-Ain Soomro 《International journal of phytoremediation》2019,21(4):352-363
Contamination of chromium signifies one of the major threats to soil system. Phytoremediation is a promising technique to reclaim metal-contaminated soil using plants which are capable to tolerate and accumulate heavy metals within in their tissues. The experiment reported in this article was carried out with six biofuel plant species, Cyamopsis tetragonoloba, Glycine max, Avena sativa, Abelmoschus esculentus, Sesamum indicum and Guizotia abyssinica, were subjected to eight Cr concentrations (0.5, 2.5, 5, 10, 25, 50, 75 and 100?mg kg?1 soil) to investigate Cr toxicity, tolerance and accumulation. After 12?weeks of experiment, Cr phytotoxicity on morphological and biochemical parameters were evaluated. For six plant species, seed germination and most of growth parameters were significantly (p?0.05) reduced under high Cr stress. Chlorophyll contents were also decreased with increased Cr concentrations. Accumulation of Cr was higher in roots than shoot in all studied plants. Significant Cr accumulation was in the order of C. tetragonoloba?>?A. sativa?>?A. esculentus?>?S. indicum?>?G. max?>?G. abyssinica. Bioconcentration factor, bioaccumulation coefficient, translocation factor and phytoremdiation ratio suggested that C. tetragonoloba, A. sativa and A. esculentus being more tolerant; having higher Cr accumulation and could be a high efficient plants for reclamation of Cr-contaminated soils. 相似文献