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101.
Caste systems and the division of labor they make possible are common underlying features of all social insects. Multiple extrinsic factors have been shown to impact caste composition in social insect colonies. Primer pheromones are one type of extrinsic caste-regulatory factor; they are chemical signaling molecules produced by certain colony members to impact developmental physiology of recipient nestmates. However, only limited evidence exists regarding primer pheromones and their actions in eusocial termites. In previous research we identified two soldier-produced terpenes, γ-cadinene (CAD) and γ-cadinenal (ALD), as candidate primer pheromones of the lower termite Reticulitermes flavipes. In the present study we tested hypotheses related to CAD and ALD action in recipient individuals. We examined the influences of terminally developed soldier termites on (1) CAD and ALD levels and (2) caste differentiation in developmentally totipotent workers. Our findings show CAD and ALD (respectively) are caste stimulatory and inhibitory components of chemical blends present in soldier heads, ALD levels increase significantly (10.9×) in workers only in the presence of soldiers, and soldiers can reduce developmental-hormone response thresholds of workers, presumably via ALD action. These findings provide novel evidence supporting that CAD and ALD are authentic caste-regulatory primer pheromones in Reticulitermes. 相似文献
102.
For the cockroach Leucophaea maderae the developmental profile of lipophorin (Lp) concentrations in the hemolymph was determined through the entire vitellogenic period. At mid-vitellogenesis the concentrations of Lp had risen to 6 times the level at emergence and then declined to 2/3 of such high values at ovulation. The racemic 10R,10S-JH-III bound to Lp with an affinity of K(d) = 5.76 nM and the natural enantiomer 10R-JH-III with a K(d) = 1.60 nM. Injections of anti-Lp into mated females caused a significantly reduced rate of oocyte growth and a substantial degree of oosorption. Injections of gamma-globulin did not significantly reduce oocyte growth and caused only a small number of oocytes to resorb. Starvation after mating had similar effects as treatment with anti-Lp. Because of the high affinity of JH to Lp and since Lp occurs in micromolar concentrations during vitellogenesis one can assume that practically all JH is bound and not available for hydrolysis by the JH esterases. Lp appears to function as an inhibitor of JH metabolism by the JHEs through substrate depletion. One may conclude that a normal rate of egg growth is only achieved when titers of Lp exceed those of JH and remove major portions of this substrate from degradation by the JHEs. 相似文献
103.
Burying beetles (Nicrophorus orbicollis) are unusual in that to breed they require an unpredictable and valuable resource, a small carcass. Thus the timing of reproduction is unpredictable and beetles' physiological response must be fast. We hypothesized that their pattern of vitellogenin (Vg) synthesis might reflect these requirements. We examined the expression of two Vg genes (sequenced for this study) during sexual maturation and through a reproductive bout. Vg-mRNA, juvenile hormone (JH) titers, ovarian development, and hemolymph concentrations of Vg were quantified in the same individuals. All four variables gradually increased during maturation to peak 15-20 days after eclosion. Twelve hours after the discovery of a carcass, a few hours before oviposition, mRNA was high, hemolymph Vg had decreased, JH and ovarian weight had increased. After oviposition, mRNA was low, hemolymph Vg concentrations and JH were high. This is consistent with our hypothesis that beetles produce and store Vg in the hemolymph prior to the discovery of a breeding resource and replace it quickly. Partial regression of these variables (with the effect of time removed) indicated that JH was not correlated with mRNA, hemolymph Vg, or ovarian weight at any time. Thus the role of JH as a gonadotropin remains unclear. 相似文献
104.
Yagi KJ Kwok R Chan KK Setter RR Myles TG Tobe SS Stay B 《Journal of insect physiology》2005,51(4):357-365
In the subterranean termite Reticulitermes flavipes, allatostatins (ASTs) with the C-terminus Phe-Gly Leu-amide were localized by immunocytochemistry with antibody against a cockroach AST, Dippu AST-7. AST-immunoreactivity occurred in the corpus cardiacum and corpus allatum and in the lateral and medial neurosecretory cells of the brain that innervate these organs as well as in many other nerve cells of the brain. This was observed in workers, nymphs, soldiers and secondary reproductives. A radioimmunoassay, using anti-Dippu AST-11, demonstrated about 40 fmole equivalents of AST in brains of soldiers and secondary reproductives. The product of the corpora allata in this species was determined to be juvenile hormone III. Its synthesis by corpora allata of secondary reproductives, determined by in vitro radiochemical assay, was inhibited in a dose-dependent fashion by two cockroach allatostatins, Dippu AST-7 and Dippu AST-11. Thus, as in cockroaches and crickets, allatostatin-containing nerves innervate the corpora allata of this termite species and their production of juvenile hormone is inhibited by these neuropeptides. 相似文献
105.
JH III esterase and JH III epoxide hydrolase (EH) in vitro activity was compared in whole body Trichoplusia ni homogenates at each stage of development (egg, larva, pupa and adult). While activity of both enzymes was detected at all ages tested, JH esterase was significantly higher than EH activity except for day three of the fifth (last) stadium (L5D3). For both enzymes, activity was highest in eggs. Adult virgin females had 4.6- and 4.0-fold higher JH esterase and EH activities, respectively, than adult virgin males. JH III metabolic activity also was measured in whole body homogenates of fifth stadium T. ni that were fed a nutritive diet (control) or starved on a non-nutritive diet of alphacel, agar and water. With larvae that were starved for 6, 28 and 52 h, EH activity per insect equivalent was 48%, 5% and 1%, respectively, of the control insects. At the same time points, JH esterase activity levels in starved T. ni were 29%, 4% and 3% of that of insects fed the nutritive diet. Selected insect hormones and xenobiotics were administered topically or orally to fifth stadium larvae for up to 52 h, and the effects on whole body EH and JH esterase activity analyzed. JH III increased the JH III esterase activity as high as 2.2-fold, but not the JH III EH activity. The JH analog, methoprene, increased both JH esterase and EH activity as high as 2.5-fold. The JH esterase inhibitor, 3-octylthio-1,1,1-trifluoropropan-2-one (OTFP), had no impact on EH activity. The epoxides trans- and cis-stilbene oxide (TSO and CSO) in separate experiments increased the EH activity approximately 2.0-fold. TSO did not alter JH esterase levels when topically applied, but oral administration reduced activity to 70% of the control at 28 h, and then increased the activity 1.8-fold at 52 h after the beginning of treatment. CSO had no effect on JH esterase activity. Phenobarbital increased EH activity by 1.9-fold, but did not change JH esterase levels. Clofibrate and cholesterol 5alpha,6alpha-epoxide had no effect on EH. JH esterase activity also was not affected by clofibrate, but cholesterol 5alpha,6alpha-epoxide reduced the JH esterase activity to 60-80% of the control. The biological significance of these results is discussed. 相似文献
106.
107.
The extracellular matrix component collagen type VI demonstrates potent growth-stimulatory effects and has been associated
with aggressive tumour growth. Although, juvenile angiofibromas (JAs) often exhibit an aggressive growth pattern, the collagen
type VI expression of this fibrovascular tumour has not been addressed so far. RT-PCR, Western blot analysis and immunohistochemistry
were used in this study to analyse collagen type VI, type VI collagen receptor subunits (integrin α1, α2, α10, α11 and β1)
and the type VI collagen receptor NG2 in JAs (N = 15) and nasal mucosa (NM, N = 8) samples. The mRNA expression of all three collagen type VI chains was found to be up-regulated significantly (P < 10−3–10−5, adjusted) in JAs compared to NM tissues. The Western blot analysis proved highly prominent collagen-type VI expression in
JAs. The ApoTome technique revealed strong collagen-type VI signals in tumour endothelium. NG2 (P < 10−3, adjusted) and α11-integrin (P = 0.04, adjusted) showed a significantly higher mRNA expression levels in JAs than in NM samples. NG2, α1-, α2- and β1-intergin
were located to tumour vessels, and additional stromal signals were observed for NG2 and α1-integrin in JAs. This study demonstrates
a prominent collagen-type VI expression in JAs. The collagen-type VI may exert an important growth stimulus in this tumour. 相似文献
108.
Mario La Mesa 《Polar Biology》2007,30(10):1219-1226
Juvenile Antarctic toothfish (Dissostichus mawsoni) collected off the South Shetland Islands (CCAMLR Subarea 48.1) were aged by counting the presumed daily increments in the
sagittal otoliths. The timing and position of the first annulus was examined for each individual fish from 10 to 13 cm TL.
Under reflected light at low magnification, the otolith sections exhibited a wide opaque nucleus, with no evidence of deposition
of the first translucent zone. The average measurement of ventral and dorsal radii of these sections were, respectively, 603
and 768 μm, consistently less than the measurements done in previously estimated 1 year old fish caught in the same area.
All specimens aged in the present study were approximately 1 year old fish, with an age range between 365 and 431 days, suggesting
a significant underestimation of age in the previous studies. The value of ageing precision indices calculated was relatively
low, supporting the reliability of the reading technique applied. Two checks were easily identified in the core of all otolith
sections, interpreted tentatively as hatching and first exogenous feeding checks. Back-calculating from the time of capture,
the hatching period of D. mawsoni was estimated to occur throughout the summer season, from November to February. In conclusion, the delay in timing of deposition
of the first translucent zone, which occurs in the second winter from hatching, should be taken into account in future studies
on ageing Antarctic toothfish, as well as in the stock assessment models based on length at age data. 相似文献
109.
Juvenile reef fish communities represent an essential component of coral reef ecosystems in the current focus of fish population
dynamics and coral reef resilience. Juvenile fish survival depends on habitat characteristics and is, following settlement,
the first determinant of the number of individuals within adult populations. The goal of this study was to provide methods
for mapping juvenile fish species richness and abundance into spatial domains suitable for micro and meso-scale analysis and
management decisions. Generalized Linear Models predicting juvenile fish species richness and abundance were developed according
to spatial and temporal environmental variables measured from 10 m up to 10 km in the southwest lagoon of New Caledonia. The
statistical model was further spatially generalized using a 1.5-m resolution, independently created, remotely sensed, habitat
map. This procedure revealed that : (1) spatial factors at 10 to 100-m scale explained up to 71% of variability in juvenile
species richness, (2) a small improvement (75%) was gained when a combination of environmental variables at different spatial
and temporal scales was used and (3) the coupling of remotely sensed data, geographical information system tools and point-based
ecological data showed that the highest species richness and abundance were predicted along a narrow margin overlapping the
coral reef flat and adjacent seagrass beds. Spatially explicit models of species distribution may be relevant for the management
of reef communities when strong relationships exist between faunistic and environmental variables and when models are built
at appropriate scales. 相似文献
110.
Summary Apterous parthenogentic females of the pea aphid,Acyrthosiphon pisum (Harris), begin to produce alate offspring soon after they have been subjected to crowding. Females which were born early in their
own parent’s reproductive period respond most strongly to crowding, producing much larger numbers of alatae than their late-born
sisters.
In contrast, the early-born daughters of most alate females do not produce winged offspring after being crowded. Some of their
later-born sisters may produce a few winged individuals, resembling in this respect the late-born daughters of the apterous
females.
Control of the production of alatae thus begins in the grandparental generation. Risk-spreading by means of differential dispersal
becomes a less uncertain venture when local populations can modify their responses to environmental changes by utilizing past
as well as present signals from their surroundings. 相似文献