排序方式: 共有62条查询结果,搜索用时 178 毫秒
11.
12.
Youming Ding Yingkang Cao Bin Wang Lei Wang Yemin Zhang Deling Zhang Xiaoyan Chen Mingxin Li Changhua Wang 《PloS one》2016,11(11)
Leptin has been implicated in tumorigenesis and tumor progression, particularly in obese patients. As a multifunctional adaptor protein, APPL1 (containing pleckstrin homology domain, phosphotyrosine binding domain, and a leucine zipper motif 1) plays a critical role in regulating adiponectin and insulin signaling pathways. Currently, high APPL1 level has been suggested to be related to metastases and progression of some types of cancer. However, the intercourse between leptin signaling pathway and APPL1 remains poorly understood. Here, we show that the protein levels and phosphorylation statues of APPL1were highly expressed in tissues from human hepatocellular carcinoma and triple-positive breast cancer. Leptin stimulated APPL1 phosphorylation in a time-dependent manner in both human hepatocellular carcinoma HepG2 cell and breast cancer MCF-7 cell. Overexpression or suppression of APPL1 promoted or attenuated, respectively, leptin-induced phosphorylation of STAT3, ERK1/2, and Akt in the cancer cells, accompanied with enhanced or mitigated cell proliferation and migration. In addition, we identified that APPL1 directly bound to both leptin receptor and STAT3. This interaction was significantly enhanced by leptin stimulation. Our results suggested that APPL1 positively mediated leptin signaling and promoted leptin-induced proliferation and migration of cancer cells. This finding reveals a novel mechanism by which leptin promotes the motility and growth of cancer cells. 相似文献
13.
Zhao J Miao J Zhao B Zhang S Yin D 《The international journal of biochemistry & cell biology》2006,38(9):1603-1613
At present, vascular endothelial cell (VEC) apoptosis induced by deprivation of fibroblast growth factor-2 (FGF-2) and serum has been well studied. But how to trigger VEC apoptosis in the presence of FGF-2 and serum is not well known. To address this question, in this study, the effects of safrole oxide on angiogenesis and VEC growth stimulated by FGF-2 were investigated. The results showed that safrole oxide inhibited angiogenesis and induced VEC apoptosis in the presence of FGF-2 and serum. To understand the possible mechanism of safrole oxide acting, we first examined the phosphorylation of Akt and the activity of nitric oxide synthase (NOS); secondly, we analyzed the expressions and distributions of Fas and P53; then we measured the activity of phosphatidylcholine specific phospholipase C (PC-PLC) in the VECs treated with and without safrole oxide. The results showed that this small molecule obviously suppressed Akt phosphorylation and the activity of NOS, and promoted the expressions of Fas and P53 markedly. Simultaneously, Fas protein clumped on cell membrane, instead of homogenously distributed. The activity of PC-PLC was not changed obviously. The data suggested that safrole oxide effectively inhibited angiogenesis and triggered VEC apoptosis in the presence of FGF-2 and serum, and it might perform its functions by suppressing Akt/NOS signal pathway, upregulating the expressions of Fas and P53 and modifying the distributing pattern of Fas in VEC. This finding provided a powerful chemical probe for promoting VEC apoptosis during angiogenesis stimulated by FGF-2. 相似文献
14.
Xu Deling Wu Yanyan Cheng Zhixing Yang Jing Ding Yanrui 《International journal of peptide research and therapeutics》2021,27(3):1933-1944
International Journal of Peptide Research and Therapeutics - Peptide drugs are generally compounds with 2–50 amino acids connected by peptide bonds and having drug effects. Because of their... 相似文献
15.
Qing Yubo Jamal Muhammad Ameen Shi Dejia Zhao Sumei Xu Kaixiang Jiao Deling Zhao Heng Li Honghui Jia Baoyu Wang Haizhen Zhao Hong-Ye Wei Hong-Jiang 《Transgenic research》2022,31(1):59-72
Transgenic Research - Leptin is a well-known adipokine that plays critical role in adiposity. To further investigate the role of leptin in adiposity, we utilized leptin overexpressing transgenic... 相似文献
16.
17.
18.
Junjun Liang Guangbing Deng Hai Long Zhifen Pan Chunping Wang Peng Cai Deling Xu Zha-Xi Nima Maoqun Yu 《Molecular breeding : new strategies in plant improvement》2012,30(1):441-451
Expression of the late embryogenesis abundant (LEA) gene is usually associated with plant response to dehydration. In this study, a drought-tolerant genotype was screened from 48 accessions of Tibetan hulless barley (Hordeum vulgare ssp. vulgare). By using virus-induced gene silencing, the influence of two LEA genes (HVA1 and Dhn6) on drought tolerance of Tibetan hulless barley was investigated. Results of quantitative real-time PCR indicated that the relative expression levels of HVA1 and Dhn6 in silenced plants were significantly reduced compared with control plants. Both HVA1-silenced and Dhn6-silenced plants showed a consequently lower survival rate than control plants under drought stress. However, only HVA1-silenced plants exhibited a significantly higher water loss rate (WLR). These results suggested that HVA1 and Dhn6 might participate in adaptive responses to water deficit in different ways. Vegetative growth of HVA1-silenced plants was significantly retarded even under optimal growth conditions, and their biomass accumulation was also much lower than that of the controls. These results indicate that HVA1 might play a role in vegetative growth of Tibetan hulless barley. 相似文献
19.
OsSERK1 regulates rice development but not immunity to Xanthomonas oryzae pv. oryzae or Magnaporthe oryzae 总被引:1,自引:0,他引:1
Shimin Zuo Xiaogang Zhou Mawsheng Chen Shilu Zhang Benjamin Schwessinger Deling Ruan Can Yuan Jing Wang Xuewei Chen Pamela C. Ronald 《植物学报(英文版)》2014,56(12):1179-1192
Somatic embryogenesis receptor kinase (SERK) proteins play pivotal roles in regulation of plant development and immunity. The rice genome contains two SERK genes, OsSerk1 and OsSerk2. We previously demonstrated that OsSerk2 is required for rice Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae (Xoo) and for normal development. Here we report the molecular characterization of OsSerk1. Overexpression of OsSerk1 results in a semi-dwarf phenotype whereas silencing of OsSerk1 results in a reduced angle of the lamina joint. OsSerk1 is not required for rice resistance to Xoo or Magnaporthe oryzae. Overexpression of OsSerk1 in OsSerk2-silenced lines complements phenotypes associated with brassinosteroid (BR) signaling defects, but not the disease resistance phenotype mediated by Xa21. In yeast, OsSERK1 interacts with itself forming homodimers, and also interacts with the kinase domains of OsSERK2 and BRI1, respectively. OsSERK1 is a functional protein kinase capable of auto-phosphorylation in vitro. We conclude that, whereas OsSERK2 regulates both rice development and immunity, OsSERK1 functions in rice development but not immunity to Xoo and M. oryzae. 相似文献
20.
With 31 provinces and cities in Mainland China selected as the research objects, the paper explores the acting mechanism of industrial cluster, urbanization and population cluster, builds a measurement index system for the spatial–temporal coupling characteristics of industrial cluster, urbanization and population agglomeration, and then measures the coupling coordination degrees of industrial agglomeration, urbanization and population agglomeration in provinces since 2000 and researches the spatial–temporal evolution and spatial differentiation characteristics of coupling coordination degree. Research results show: ① industrial agglomeration, urbanization and population agglomeration have formed a dynamically coordinated relationship with mutual influences, mutual promotion and joint development; ② the comprehensive development levels of industrial agglomeration, urbanization and population agglomeration in China are rising year by year, but the overall coupling degree and coordination degree of the three are low, but the coupling coordination relationship will improve continuously with time, still showing obvious differences in provinces and the spatial tendency of being “high in the east and low in the west”; ③ the coupling coordination presents a continuous spatial agglomeration state, and the agglomeration intensity is “high on two ends and low in the middle”, thus forming the outstanding hot-spot in areas eastern coastal regions and the outstanding cold-spot areas in western regions; ④ the government should promote industrial agglomeration, industrial structure optimization and upgrading and urbanization, and make reasonable planning for population agglomeration degree practically and effectively, to ensure the matching and coordinated development of industrial agglomeration, population agglomeration and urban bearing capacity. 相似文献