A molecular assessment of homing in the tidepool sculpin

Molecular analyses of the mitochondrial genome using restriction fragment length polymorphism (RFLP) were utilized to determine the existence of discrete populations of the tidepool sculpin Oligocottus maculosus from the Barkley Sound region of south-western Vancouver Island, British Columbia. Fifty-five haplotypes were found with three haplotypes making up 38, 16 and 8% of the total sample, respectively. Within individual tidepools, haplotype diversity ranged from 0·30 to 0·92, but haplotypes were typically closely related to one another (nucleotide diversity ranged from 0·0004 to 0·005). The majority of haplotype variation (96–97%) was located within tidepools, but a significant amount of variation ( c . 3–4%) was detected among tidepools within single islands. By contrast, there was no significant differentiation among islands or between samples from the Barkley Sound area and a single sample from north-eastern Vancouver Island. The results suggest a low level of population structure within the sample area and that the distribution of haplotypes and haplotype divergences represents a demographic expansion from a single ancestral lineage beginning between 300 000 and 1 000 000 years ago. The low level of population subdivision resolved suggests that despite their ecological specialization in tidepool habitats, tidepool sculpins are influenced by demographic and genetic processes that operate on spatial scales much larger than single tidepools or islands.     

Aerial release of CO2 and respiratory exchange ratio in intertidal fishes out of water

Synopsis Many fishes in the marine intertidal zone have the ability to exchange respiratory gases in air during brief periods out of water. Previous studies on marine amphibious fishes such as mudskippers and rockskippers have shown that they release CO₂ in air at a rate consistent with its release in aquatic respiration. Respiratory Exchange Ratios (RER, CO₂ released per O₂ consumed) are between 0.7 and 1.0, similar to Respiratory Quotients (CO₂ produced metabolically per O₂ consumed). However, previous studies of temperate intertidal fishes emerged only at low tides have been less consistent, with two species reported similar in ability to the amphibious skipper fishes, and two others reported with much lower RERs in air. This study examines 12 species of fishes, from six families (Batrachoididae, Cottidae, Gobiesocidae, Kyphosidae, Pholididae, and Stichaeidae), present in the intertidal zone of California. All 12 species exchanged O₂ and CO₂ in air. Like the amphibious skipper fishes of marine tropical waters, many intertidal fishes of the temperate zone release CO₂ during low tide emergence in quantities sufficient to match its metabolic production. These results indicate that CO₂ release is not hampered by the change in respiratory medium from water to air.     

Geographical patterns of allozymic variation in three species of intertidal sculpins

Synopsis We examined spatial patterns of allozyme variation in three species of intertidal cottids to evaluate how much gene flow occurs between geographically separate populations (most likely via passive dispersal of planktonic larvae by currents). Our results from the analysis of geographical patterns of allele frequencies and, to some extent, from comparison of allele frequencies between newly recruited young and older resident fish are consistent with the notion that sufficient gene flow occurs in these fishes to prevent significant genetic isolation of populations. From these results, we visualize evolutionary changes in populations of these species as occurring most likely over a broad geographic scale, affecting spatially separated but genetically linked populations concurrently, rather than operating independently in individual, genetically isolated populations.     

Lack of taxonomic information from parietal spine size invalidates subspecies in the Atlantic hookear sculpin Artediellus atlanticus

An analysis of 107 individuals of Atlantic hookear sculpin Artediellus atlanticus from the Barents Sea–Svalbard region and from north‐east Greenland shows that the state of the parietal spines, although not randomly distributed geographically, occurs sympatrically. They do not diagnostically describe populations. They are therefore inept for subspecies delimitation into Artediellus atlanticus atlanticus, Artediellus atlanticus corniger and Artediellus atlanticus europaeus. Sympatric occurrence of parietal spine states called for a molecular approach to look for differences at a species level. Sequencing the mitochondrial genes, cytochrome oxidase c subunit I (coI) and cytochrome b (cytb), shows no difference between individuals with different parietal spine size. Thus, there is no evidence of the spine states representing species‐level variation. The study shows that parietal spine size has no taxonomic information for this species. The patterns of variation in parietal spine size differ to some degree between the sexes.     

Genetic variability, population size and isolation of distinct populations in the freshwater fish Cottus gobio L.

Analysis of allozyme data of the European freshwater fish Cottus gobio showed marked genetic differentiation across drainage basins in northeastern Bavaria, which points to the existence of at least two cryptic taxa. Genetic variability within populations differed significantly between these two taxa, which could be due to historical (bottlenecks) or ecological reasons (population size). To distinguish between these two hypotheses we sampled 12 distinct populations from Rhine, Elbe and Danube drainages. Using allozyme data we examined the influence of population size and isolation on genetic variability within populations. We used spatial extent of populations (patch size) as a measure for population size. To estimate isolation we calculated a compound measure which took into account patch size and distance to all neighbouring populations. Both patch size and isolation were highly correlated with genetic variability, explaining ≈95% of the variance of genetic variability within populations. Furthermore, analysis of covariance suggests that the difference in genetic variability between taxa may be explained by differences in population size.     

Effects of a Severe Flood on the Movements of Japanese Fluvial Sculpin

I studied the movements of adult Japanese fluvial sculpin, Cottus pollux, in a Japanese mountain stream. An exceptionally severe flood in late September had negative impacts on refuge abundance, condition and population density of the sculpin. The mean distance moved monthly correlated positively with water discharge, but not with water temperature or with population density. Overall, the mean distance sculpins moved after the flood was significantly greater than before the flood, and sculpins tended to move into riffle-raceways after the flood. Comparisons of refuge-site limitation for adults and water depths between habitats indicated that the flood affected riffle-raceways less than pools. Fish in poorer conditions were likely to move extensively, and the condition of fish captured initially in pools deteriorated more significantly than that of sculpins captured in riffle-raceways. Movement bias into riffle-raceways by the sculpins after the flood suggests they sought suitable habitat with available refuges. The results suggest vulnerability to flood disturbance of the sculpins inhabiting the interstitial spaces of the substrate.     

Biotic and Abiotic Influences on Nest-hatching Outcome in the Japanese Fluvial Sculpin, Cottus pollux

Synopsis I examined the interrelationships among the timing of reproduction, physical characteristics of nests and hatching outcome of male Japanese fluvial sculpin, Cottus pollux, in a Japanese mountain stream with flood disturbance. Breeding took place from mid-February to early April, with bimodal peaks of breeding activity. Male body size had a significant effect on the timing of initial reproduction and mating success. Hatching outcome, by contrast, was not affected by male body size and male mating success. The timing of flood occurrence varied between years, and most nest-hatching failure was observed within 1 week after floods. Failed nests were exposed to faster current velocity than successful nests, and degree-days of failed nests negatively correlated with the magnitude of flood flow experienced. These data suggest that the timing and magnitude of floods substantially affected hatching outcome of the species. I also discuss the adaptive significance of bimodal breeding activity of males from life-history perspective in the face of variable stream flow conditions.     

The angular position of a refuge affects escape responses in staghorn sculpin Leptocottus armatus

The effect of the presence and angular position of a refuge on the direction and kinematics of mechanically‐induced escape responses was observed in staghorn sculpins Leptocottus armatus using high–speed video. The results showed that the angular position of the refuge did not affect locomotor performance (speed and acceleration), although it did affect the escape trajectories. Therefore, the angular position of a refuge can modulate the direction taken by the L. armatus during the early stages of their escape response and this response can be affected by both repulsive (i.e. threats) and attractive (i.e. refuges) points of reference.     

Troglomorphic Sculpins of the Cottus Carolinae Species Group in Perry County, Missouri: Distribution, External Morphology, and Conservation Status

The existence of cavernicolous sculpin (here allocated to Cottus carolinae, banded sculpin, and referred to as grotto sculpin), in the karst regions of Perry County, Missouri, first came to our attention in 1991. Examination of 35 caves in Missouri, 96 in Illinois, 17 in Tennessee, two in Indiana, and 11 in Arkansas revealed that banded sculpin are common in cave habitats; however, grotto sculpin are limited to two karst areas of Perry County, Missouri, where they are known from only six cave systems. These caves and their streams are extensive and apparently provide a unique habitat compared to other karst systems; this may be a critical factor in the present restricted distribution of the grotto sculpin. Grotto sculpin occupy pools and riffles of cave streams, and occur over a variety of substrates, from sediment to breakdown. Density estimates in Mystery and Running Bull caves were 0.29 and 0.63 individuals m^-2, respectively. Grotto sculpin have small eyes (1–6% SL vs. 6–10% SL in epigean samples), significantly reduced pigmentation (including nearly complete loss of dorsal saddles), a reduction in pelvic fin ray number (from 4+4 elements to often 4+3 , or 3+3), and enlarged cephalic lateralis pores (e.g., mandibular pores of cavernicolous samples are 2–3 times those of epigean stream samples). Multivariate analyses of body shape revealed statistically significant separation of epigean and hypogean samples, with eye size highly variable, but smallest in the Running Bull Cave population. We interpret these results as representative of losses associated with long-term cave habitation. Caves of Perry County provide ample habitat for grotto sculpin, but because the caves are located downgradient of the city of Perryville and an intensively farmed landscape, point and non-point source pollution threaten their continued existence. Escape of farm-pond fishes through the extensive sinkhole network in Perry County has increased potential predation pressure on grotto sculpin by channel catfish, Ictalurus punctatus, and other species normally excluded from cave environments.