用阿片受体放射自显影法研究电针对电惊厥的作用

采用大鼠脑切片的阿片受体结合和放射自显影技术,分析了与电惊厥发作有关的脑内结构及其阿片受体在电针抑制惊厥发作时的变化。图象处理和光密度测定的结果表明,反复电惊厥使尾核、海马、缰核和杏仁核内的阿片受体密度增加。而经电针处理后再给予电惊厥,与电惊厥组相比,海马、缰核和杏仁核内的受体密度降低,在尾核亦显示了受体密度降低的趋势。这一结果为这些结构内的阿片受体参与电惊厥发作和针刺制痫的过程提供了形态学方面的证据。     

Probing the optical properties and luminescence mechanism of a UV‐emitting SrBPO5:Ce3+ phosphor

Ce‐doped (1 × 10^?5 to 3.0 mol%) SrBPO₅ phosphors were synthesized using a conventional solid‐state reaction route at 1273 K in an air atmosphere. Phase and morphology of the samples were studied from powder X‐ray diffraction (XRD) patterns and scanning electron microscope (SEM) micrographs, respectively. The band gap energies of the pure and Ce‐doped SrBPO₅ phosphors were calculated from the recorded diffuse reflectance spectra. Photoluminescence (PL) and Ce³⁺ lifetime were recorded at 300 and 77 K. Photoluminescence lifetime measurements revealed two‐lifetime values for Ce³⁺ at both 300 K (17 and 36 nsec) and 77 K (12 and 30 nsec), suggesting the presence of two different environments around Ce³⁺. Time‐resolved emission spectroscopy (TRES) studies confirmed the presence of Ce³⁺ in two different environments. In addition, SrBPO₅:Ce exhibited intense UV emission, signifying its possible use as an efficient sensitizer for solid‐state lighting applications. The effect of γ‐irradiation on PL was also determined. Thermally stimulated luminescence (TSL) glow curves of the γ‐irradiated phosphor, along with trap parameters, dose–response, and the possible TSL mechanism were also investigated. Positron annihilation lifetime spectroscopy was carried out to probe defects present in undoped and Ce‐doped SrBPO₅.     

MiR-141–3p promotes hypoxia-induced autophagy in human placental trophoblast cells

Preeclampsia (PE) is a pregnancy-specific disorder and a significant contributor to maternal, fetal and neonatal morbidity and mortality worldwide. Its pathogenesis is generally accepted as insufficient trophoblast invasion of the maternal endometrium and inadequate remodeling of the maternal spiral arteries. These impairments lead to elevated levels of hypoxia and oxidative stress. Autophagy has become a highly researched field in obstetrics, and this process may be essential for preimplantation development beyond the four- and eight-cell stages, and for blastocyst survival, extra-villous trophoblast functions, invasion and vascular remodeling. Several studies have shown that autophagy activation, shown by an increase in autophagy vacuoles or microtubule-associated protein 1 A/1B-light chain 3 (LC3) dots, was more common in PE than in normal pregnancy. Thus, changes in autophagic status are seen in preeclamptic placentas. MicroRNA-141–3p (miR-141–3p), a multifunctional miRNA, is involved in a variety of physiological and pathological processes, including PE and autophagy. However, the influence of miR-141–3p on autophagy regulation in trophoblast cells has yet to be described. Therefore, the objective of our study was to investigate the role of miR-141–3p in autophagy induced by hypoxia in human placental trophoblast cells. Our results found that hypoxia induced autophagy in trophoblast cells and dramatically elevated the expression of miR-141–3p. Overexpression of miR-141–3p improved autophagic activity, whereas low expression of miR-141–3p inhibited autophagic activity. Therefore, our data demonstrated that miR-141–3p promoted hypoxia-induced autophagy in placental trophoblast cells, which may be related to the development of preeclampsia.     

肯氏相思~(60)Co-r辐照诱变研究

采用不同剂量（3000、5000、7000Rad）(60)Co－r辐照肯氏相思种子，选育出诱变化株（M1），以M1的种子（M2）扩种试验。观测M1和M2代的形态、物候，并测定过氧化物酶同功酶带谱、酶活性、蛋白质含量，结果表明，各处理的优株与对照都存在差异。在相同生态条件下，M1代林木生长量和民代地上部生物量分别高于对照见11.48％～21．64％和14．62％～73．98％，以5000Rad辐照剂量最优，其次为7000Rad。访变个体后代表现树干直、花果期提早和种子小。     

Ce−ZSM-5: A Highly Capable Catalyst for the Construction of Acridines in Water and Their Computational Study against DNA Gyrase Protein

Cerium doped ZSM-5 (Ce−ZSM-5) as an environmentally benign and reusable catalyst for the construction of acridines in aqueous medium. This method produced corresponding acridines with good yields and shorter reaction times. Also avoids the use of hazardous solvents and involves a simple work-up process. The solid catalyst was prepared by doping Ce ions with ZSM-5 (Zeolite Socony Mobil-5) and confirmed by XRD, BET SA-PSD and SEM. The synthesised acridine derivatives were confirmed by ¹H-NMR, ¹³C-NMR and FT-IR spectroscopic data. The docking studies of the synthesised compounds are performed by the PyRx auto dock tool against DNA gyrase protein. The products 5a and 6d are found to be the best fit ligands against DNA gyrase protein.     

Studies on luminescence from a cerium‐doped strontium stannate phosphor

The crystal structure and morphology of Ce³⁺‐doped SrSnO₃ materials prepared using the solid‐state reaction method were extensively characterized using experimental techniques. X‐Ray diffraction results show that the cerium substitution of strontium does not change the structure of the strontium stannate. Raman spectroscopy was used to investigate the microstructures and lattice vibrations. Environmental scanning electron microscopy images showed that phosphors aggregate and their particles form irregular shapes. SrSnO₃ exhibits an intense green emission with a broad band originating from the 5d¹ → 4f¹ transition of cerium. It was observed that, after exposure to beta‐irradiation, the glow curve of this material has two broad thermoluminescence peaks, one centered at ~ 127°C and the other at ~ 245°C for a heating rate of 5 K/s. The kinetic parameters, which include the frequency factor and the activation energy of the material, were calculated using Chen's method, after beta‐irradiation. The fading and reusability of the phosphor were also studied and it was found that the phosphor is suitable for radiation dosimetry. Copyright © 2014 John Wiley & Sons, Ltd.     

Effect of (Ce,Dy) co-doping on the microstructural,optical, and photoluminescence characteristics of solution combustion synthesized ZnO nanoparticles

Solution combustion synthesized ZnO nanoparticles that were Ce doped, Dy doped or co-doped at varying dopant concentrations were characterized for their microstructural, optical, and photoluminescence (PL) characteristics. The synthesized nanoparticles matched the standard hexagonal wurtzite structure of ZnO. The lattice fringes in the high-resolution transmission electron micrographs and the bright spotty rings in the selected area electron diffraction patterns authenticated the high crystallinity of the nanoparticles. The diffuse reflectance spectroscopy resolved the energy bandgap for the undoped ZnO as 3.18 eV, which decreased upon doping and co-doping. A sharp narrow ultraviolet emission peak at ~398 nm that originated from excitonic recombination was found in the PL spectra of the nanoparticles. The visible emission peaks in the PL spectra were assigned to the f–d and f–f electron transitions of Ce³⁺ and Dy³⁺ ions, respectively, in addition to different native defects in ZnO. The visible emissions (blue, yellow, and red) improved upon (Ce, Dy) co-doping, therefore (Ce, Dy) co-doped ZnO nanoparticles can be considered a promising luminescent material for the development of energy-saving light sources.     

Energy transfer induced white‐light‐emitting phosphor by co‐doping Ce3+, Tb3+ and Mn2+ into the single Ba9Lu2Si6O24 host

In the Ba₉Lu₂Si₆O₂₄ (BLS) host, Ce³⁺ shows cyan emissions peaking at 490 nm under 400 nm excitations. BLS:Tb³⁺ only can be effectively excited by 254 nm light and gives rise to green emissions at 553 nm. However, both the cyan and green emissions can be obtained in BLS:Ce³⁺,Tb³⁺ under 400 nm excitations due to effective energy transfers from Ce³⁺ to Tb³⁺. BLS:Mn²⁺ shows red emissions peaking at 610 nm under 414 nm excitations. By co‐doping Ce³⁺, Tb³⁺ and Mn²⁺, tunable full‐color emissions were obtained. The BLS:0.3Ce³⁺,0.6Tb³⁺,0.15Mn²⁺ single phosphor exhibits a white light with a high color rendering index of 85 and a correlated color temperature of 5480 K under 400 nm excitation.     

人鼻咽癌上皮细胞系CNE1条件培液具有转化生长抑制因子活力

生长因子是细胞间通讯的语言之一,是调节细胞生长的重要媒介物。越来越多的证据证明:生长因子是多功能多肽,一种组织内细胞群落的生长控制需要通过一系列生长因子与