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81.
A parentage evaluation test in North American Elk (Wapiti) using microsatellites of ovine and bovine origin 总被引:6,自引:0,他引:6
DNA microsatellites originally detected in sheep and cattle are also detectable in North American elk (Wapiti) using polymerase chain reactions. We have developed a parentage test in elk using eleven microsatellite markers that are highly polymorphic and informative. 相似文献
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83.
The role of marine reserves in the replenishment of a locally impacted population of anemonefish on the Great Barrier Reef 下载免费PDF全文
Mary C. Bonin Hugo B. Harrison David H. Williamson Ashley J. Frisch Pablo Saenz‐Agudelo Michael L. Berumen Geoffrey P. Jones 《Molecular ecology》2016,25(2):487-499
The development of parentage analysis to track the dispersal of juvenile offspring has given us unprecedented insight into the population dynamics of coral reef fishes. These tools now have the potential to inform fisheries management and species conservation, particularly for small fragmented populations under threat from exploitation and disturbance. In this study, we resolve patterns of larval dispersal for a population of the anemonefish Amphiprion melanopus in the Keppel Islands (southern Great Barrier Reef). Habitat loss and fishing appear to have impacted this population and a network of no‐take marine reserves currently protects 75% of the potential breeders. Using parentage analysis, we estimate that 21% of recruitment in the island group was generated locally and that breeding adults living in reserves were responsible for 79% (31 of 39) of these of locally produced juveniles. Overall, the network of reserves was fully connected via larval dispersal; however, one reserve was identified as a critical source of larvae for the island group. The population in the Keppel Islands also appears to be well‐connected to other source populations at least 60 km away, given that 79% (145 of 184) of the juveniles sampled remained unassigned in the parentage analysis. We estimated the effective size of the A. melanopus metapopulation to be 745 (582–993 95% CI) and recommend continued monitoring of its genetic status. Maintaining connectivity with populations beyond the Keppel Islands and recovery of local recruitment habitat, potentially through active restoration of host anemone populations, will be important for its long‐term persistence. 相似文献
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The camel racing industry would have added value in being able to assign parentage with high certainty. This study was aimed at assessing and applying microsatellite multiplexes to construct a parentage testing system for camels. An efficient system of 17 loci from 700 camel samples was used to construct a database of unrelated adults. Based on this, we estimated measures of polymorphism among the markers. In three multiplex reactions, we detected a total of 224 alleles, with 5–23 alleles/locus (mean = 13.18 ± 6.95 SD) and an average heterozygosity (HE) of 0.54 (range 0.032–0.905). The total parentage exclusion probability was 0.99999 for excluding a candidate parent from parentage of an arbitrary offspring, given only the genotype of the offspring, and 0.9999 for excluding a candidate parent from parentage of an arbitrary offspring, given the genotype of the offspring and the other parent. We used 15 juveniles for parentage testing, as well as 17 sires (bull camels) and 21 dams (cows). In the case of parentage assignment, the microsatellite panel assigned all 15 offspring parentage with high confidence. Overall, these findings offer a set of microsatellite markers that are easy, simple and highly informative for parentage testing in camels. 相似文献
85.
We introduce toleranced models (TOMs), a generic and versatile framework meant to handle models of macromolecular assemblies featuring uncertainties on the shapes and the positions of proteins. A TOM being a continuum of nested shapes, the inner (resp. outer) ones representing high (low) confidence regions, we present topological and geometric statistics assessing features of this continuum at multiple scales. While the topological statistics qualify contacts between instances of protein types and complexes involving prescribed protein types, the geometric statistics scale the geometric accuracy of these complexes. We validate the TOM framework on recent average models of the entire nuclear pore complex (NPC) obtained from reconstruction by data integration, and confront our quantitative analysis against experimental findings related to complexes of the NPC, namely the Y-complex, the T-complex, and the Nsp1-Nup82-Nup159 complex. In the three cases, our analysis bridges the gap between global qualitative models of the entire NPC, and atomic resolution models or putative models of the aforementioned complexes. In a broader perspective, the quantitative assessments provided by the TOM framework should prove instrumental to implement a virtuous loop "model reconstruction-model selection", in the context of reconstruction by data integration. 相似文献
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In the majority of birds and mammals, social monogamy is not congruent with genetic monogamy. No research to date has compared social and genetic monogamy in amphibians. We analysed paternity in clutches of red-backed salamanders (Plethodon cinereus), a species in which social monogamy has been demonstrated in the laboratory, and 28% of individuals in the forest are found in male-female pairs in the noncourtship season. We collected 16 clutches of eggs of P. cinereus in the southern Appalachian Mountains of Virginia and collected tail clippings from attending mothers. We genotyped embryos and adults at five microsatellite loci in order to analyse paternity of clutches. Most clutches (84.6%) had multiple sires, with two to three sires per clutch. In this study, 25% of clutches had males in addition to females attending eggs. None of the mothers of these clutches were genetically monogamous. All attending males sired some of the offspring in the clutch that they attended (between 9% and 50%) but never sired a majority in that clutch. We conclude that, at least in this population, social monogamy in P. cinereus is not concomitant with genetic monogamy. 相似文献
87.
ANTHONY B. WILSON 《Molecular ecology》2009,18(7):1307-1309
Genetic analyses of realized reproductive success have fundamentally changed our understanding of mating behaviour in natural systems. While behavioural ecologists have long been interested in what factors influence mating behaviour, early studies were limited to direct observations of matings and thus provided an incomplete picture of reproductive activity. Genetic assessments of parentage have revolutionized the study of reproductive behaviour, revealing that many individuals engage in extra‐pair copulations ( Griffith et al. 2002 ) and that social mating partners frequently invest substantial resources into raising offspring that are unrelated to one or both of them ( Avise et al. 2002 ). While these findings have changed the way we think about reproductive behaviour, most investigations of genetic parentage have been restricted to single populations at a single point in time, obscuring spatial and/or temporal variation in mating behaviour and limiting our ability to determine how environmental changes can lead to shifts in mating strategies. In this issue of Molecular Ecology, Mobley & Jones (2009) compare genetic mating behaviour across five populations of Syngnathus floridae ( Fig. 1 ), a widespread species of pipefish distributed along the Gulf‐ and Atlantic Coasts of North America. The authors document how genetic mating behaviour varies across space in S. floridae and identify correlations between reproductive variation and particular ecological characteristics. Mobley & Jones’ paper is one of an increasing number of studies which address the question of how ecological variables influence mating behaviour, and highlights how our understanding of mating system variation and evolution is likely to expand through the wider application of high‐throughput parentage assessment in a comparative context.
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We isolated and characterized 10 polymorphic microsatellite loci in Drosophila hydei. The number of alleles per locus ranged from 3 to 8 (N = 23 individuals). Polymorphic information content ranged from 0.316 to 0.750 and observed heterozygosity from 0.261 to 0.913. These markers will be valuable in studies of sexual selection and parental investment in D. hydei. 相似文献
90.
The white‐crowned sparrow, Zonotrichia leucophrys, has served as a model species for studies of song and reproductive physiology. Here, we describe primers for 21 novel microsatellite loci isolated from the Puget Sound subspecies, Zonotrichia leucophrys pugetensis, which will be useful for parentage and population genetic analyses. Based on genotypes from seven to 22 adult birds from one population, the average number of alleles per locus was 10.9 (four to 21 alleles) and observed heterozygosity varied from 0.50 to 1.00. All loci also amplified products in at least one of three other passerine species tested. 相似文献