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41.
The relationship between time of inoculation with cucumber mosaic cucumovirus (CMV) and the growth, seed production and rate of seed transmission of virus in lupin (Lupinus angustifolius cv. Illyarrie) was studied in field-grown plants. Plants inoculated at the seedling stage (2 days post-emergence) showed 45% mortality. Plants infected through the seed were more stunted than plants inoculated at the seedling stage. Plants inoculated up to the mid-vegetative growth stage (58 days post-emergence) yielded ≤ 27% of the dry matter and ≤ 9% of the seed of healthy plants. Late inoculation (114 days post-emergence) did not affect dry matter yield, but reduced seed yield to 75% of that of healthy plants. Rate of seed transmission depended on the time of inoculation of plants. The maximum rate was 24.5% for plants that were inoculated at the mid-vegetative growth stage (58 days post-emergence). However, early inoculation caused a large reduction in seed yield, and it was shown that plants inoculated at the beginning of flowering (94 days post-emergence) produced greater numbers of infected progeny than plants inoculated at earlier or later times. No relationship was observed between seed weight and transmission of CMV. Infectious CMV was recovered from the embryo, but not from the testa. A simple seed transmission model was used to evaluate several hypothetical epidemics and to determine the time of inoculation which results in greatest rates of seed transmission of CMV. For example, when fewer than 73% of plants in a crop become infected with CMV, then the rate of transmission of virus in crop seeds will be greatest when inoculations are at the beginning of flowering. 相似文献
42.
Joshua R. Kohn Spencer C. H. Barrett 《Evolution; international journal of organic evolution》1994,48(5):1576-1594
Floral traits that increase self-fertilization are expected to spread unless countered by the effects of inbreeding depression, pollen discounting (reduced outcross pollen success by individuals with increased rates of self-fertilization), or both. Few studies have attempted to measure pollen discounting because to do so requires estimating the male outcrossing success of plants that differ in selfing rate. In natural populations of tristylous Eichhornia paniculata, selfing variants of the mid-styled morph are usually absent from populations containing all three style morphs but often predominate in nontrimorphic populations. We used experimental garden populations of genetically marked plants to investigate whether the effects of population morph structure on relative gamete transmission by unmodified (M) and selfing variants (M‘) of the mid-styled morph could explain their observed distribution. Transmission through ovules and self and outcross pollen by plants of the M and M’ morphs were compared under trimorphic, dimorphic (S morph absent), and monomorphic (L and S morphs absent) population structures. Neither population structure nor floral morphology affected female reproductive success, but both had strong effects on the relative transmission of male gametes. The frequency of self-fertilization in the M' morph was consistently higher than that of the M morph under all morph structures, and the frequency of self-fertilization by both morphs increased as morph diversity of experimental populations declined. In trimorphic populations, total transmission by the M and M' morphs did not differ. The small, nonsignificant increase in selfing by the M' relative to the M morph was balanced by decreased outcross siring success, particularly on the S morph. In populations lacking the S morph, male gamete transmission by the M' morph was approximately 1.5 times greater than that by the M morph because of both increased selfing and increased success through outcross pollen donation. Therefore, gamete transmission strongly favored the M' morph only in the absence of the S morph, a result consistent with the distribution of the M' morph in nature. This study indicates that floral traits that alter the selfing rate can have large and context-dependent influences on outcross pollen donation. 相似文献
43.
Núria Saperas Juan Ausio Domènec Lloris Manel Chiva 《Journal of molecular evolution》1994,39(3):282-295
Fish protamines are highly specialized molecules which are responsible for chromatin condensation during the last stages of spermatogenesis (spermiogenesis). However, not all fish contain protamines in their sperm nuclei; rather, there seems to be a random distribution of protamines within this group. The origin of this sporadic presence of protamines in the sperm and its significance have not yet been precisely determined. In this paper we have conducted an exhaustive survey of the literature available on the different types of nuclear protein composition of the sperm of teleost fish in order to try to correlate these data with what is presently known about the taxonomy of this group. The results of this analysis have allowed us to make the following observations. The divergence between protamines and histones has occurred several times during the evolution of the bony fish. However, the relative frequency of this divergence is almost negligible during the differentiation of genera and species (intrafamily variation) and is very small during the differentiation of families (interfamily variation). Nevertheless, the divergence is very noticeable among the different orders. It is therefore possible to conclude from all this that the sporadic distribution of protamines in bony fish is not a random event as initially believed. Furthermore, such a heterogeneous distribution of protamines cannot be easily accounted for by a mechanism of horizontal retroviral transmission through repeated and independent acquisition of a prot amine gene as has been recently proposed (Jankowski, Stater, Dixon (1986) J Mol Evol 23:1–10). Rather, it could possibly be explained by a repeated and independent loss of the expression of the protamine gene (or loss of the gene itself) which mainly occurred during the diversification of the orders of this group.Correspondence to: J. Ausio 相似文献
44.
Aphid activities during sieve element punctures 总被引:13,自引:0,他引:13
Aphid salivation in sieve elements and phloem sap ingestion were linked to waveforms in the Electrical Penetration Graph (EPG).
Non-viruliferousRhopalosiphum padi (L.) (Hemiptera, Aphididae) on barley yellow dwarf virus (BYDV) infected wheat could acquire the virus, which was used as
an indication for phloem sap ingestion, whereas virus inoculation by viruliferous aphids on healthy plants was associated
with salivation in sieve elements or other phloem cells. Probing was monitored and the waveforms recorded were related to
ELISA results of test plants. The EPG patterns A, B, and C are indicative of the stylet pathway phase, whereas patterns E1
and E2 reflect the phloem (sieve element) phase with an unknown activity (E1) or with ingestion and concurrent salivation
(E2). Aphids showing pathway and E1 rarely acquired virus, suggesting that little or no phloem sap ingestion can occur during
these patterns, whereas those showing additionally pattern E2 did so substantially, indicating phloem sap ingestion. The main
pattern related to virus inoculation was E1, although some aphids were able to inoculate plants during pathway. Pattern E1
clearly reflects the most important salivation into sieve elements. Pattern E2 had no clear contribution to virus inoculation,
supporting the present hypothesis that during this pattern the saliva is mixed with the phloem sap in the single canal at
the stylet tips and ingested immediately, without reaching the plant tissue. Sustained sap ingestion did not affect virus
inoculation. So, BYDV inoculation mainly occurs during the first period of a sieve element puncture which is always formed
by E1. Implications on persistent virus transmission are discussed. 相似文献
45.
C. David Pauza Peter Emau Maria S. Salvato Parul Trivedi Debra MacKenzie Miroslav Malkovsky Hideo Uno Kevin T. Schultz 《Journal of medical primatology》1993,22(2-3):154-161
Intrarectal inoculation of rhesus monkeys with low doses of SIVmac led to a prolonged clinical and virological latency that was not observed for high intrarectal doses or for intravenous inoculation. Animals infected intrarectally with low virus doses remained negative for serum antibody responses to SIV for at least one year even though they readily transferred SIV to naive recipients via transfusion of whole blood. 相似文献
46.
Seed transmission of turnip yellow mosaic virus in winter turnip and winter oilseed rapes 总被引:1,自引:0,他引:1
This is the first record of seed transmission of turnip yellow mosaic virus (TYMV) in oilseed and turnip rapes. The seed transmission of TYMV in a naturally infected winter turnip rape (Brassica napus var. silvestris) cultivar Perko PVH was investigated. By ELISA 1.6%, 3.2% and 8.3% seed transmission of the virus was found in seed of plants from three localities. The proportion of infected seeds produced by artificially infected plants of winter oilseed rape (Brassica napus ssp. oleifera) and winter turnip rape cultivars was determined. The virus transmission rate, expressed as the proportion of virus-infected plants which germinated from the seed was for the oilseed rape cvs Jet Neuf 0.1%, Solida 0.4%, Silesia 0.8%, Darmor 1.2%, SL-507 0.2%, SL-509 0.0% and for the winter turnip rape cv. Perko 1.5%. ELISA cannot be used in direct tests on bulk seed lots to estimate proportion of infected seed, but must be used on germinated seedlings. 相似文献
47.
The cultural reproduction of lithic technology, long an implicit assumption of archaeological theories, has garnered increasing attention over the past decades. Major debates ranging from the origins of the human culture capacity to the interpretation of spatiotemporal patterning now make explicit reference to social learning mechanisms and cultural evolutionary dynamics. This burgeoning literature has produced important insights and methodological innovations. However, this rapid growth has sometimes led to confusion and controversy due to an under-examination of underlying theoretical and methodological assumptions. The time is thus ripe for a critical assessment of progress in the study of the cultural reproduction of lithic technology. Here we review recent work addressing the evolutionary origins of human culture and the meaning of artifact variation at both intrasite and intersite levels. We propose that further progress will require a more extended and context-specific evolutionary approach to address the complexity of real-world cultural reproduction. 相似文献
48.
49.
50.
Ch. Leuschner 《植被学杂志》1993,4(2):255-262
Abstract. The availability of maj or plant resources was investigated in three vegetation types that were assumed to represent different stages of a secondary succession on heathland on the Lüneburger Heide, northwestern Germany. Canopy transmission and absorption of photosynthetically active radiation (PAR), soil-water availability, and nutrient (Ca, K, Mg, N, P) availability were monitored with high spatial and temporal resolution in (1) a Calluna vulgaris heathland, (2) a pioneer birch-pine forest and (3) a late-successional oak-beech forest, situated close to each other on comparable geological substrate (diluvial). Mean fractional transmission of PAR during summer decreased from 0.48 in the heathland to 0.04 in the oak-beech forest while the fractional canopy absorption increased from 0.49 to 0.92. Soil-water availability as indicated by the soil-water potential, was significantly influenced by differential canopy interception loss and characteristic rooting patterns in the three vegetation types. Annual mean nutrient concentrations in the equilibrium soil solution were similar or, for some elements, increased from the heathland to the birch-pine and the oak-beech forest despite a growing demand. A marked increase was found for the total nutrient pools in the soil-organic layer between early and late successional vegetation types. On the poor glacial parent material, nutrient pools seem to be strongly dependent on stand productivity and litter production which increased with succession. Thus, for nutrients, facilitation seems to be important in this type of succession. 相似文献