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81.
用HBsAgRPHA做为模式,选用羊、火鸡、鹅、人O型血球为载体,醛化后分成致敏和不致敏两种,与异嗜性抗体、中国生物制品药品检定所的参比品等作凝集试验。结果表明,无论特异性还是灵敏度都以人O型血球为最佳。在此基础上,设计了五种醛化方法处理人O型血球,共得出18个醛化样品,经73种不同的比较试验,筛选出最佳醛化方法为改良丙酮醛──甲醛法,采用该法醛化后血球为褐色,阴性血清和空白对照背景清晰,血球沉集点致密,下滑程度好。致敏抗—HBsMcAb后,使HBsAg灵敏度达到1~2ng/ml。为制备高质量的血凝试剂提供了最佳载体。 相似文献
82.
不同生境区稻田节肢动物群落相似性分析 总被引:1,自引:0,他引:1
根据不同生境区稻田节肢动物群落系统调查结果,应用组平均聚类分析法及主成分分析法,讨论节肢动物群落的相似(相异)性问题。结果表明:生境条件及管理措施的差异对群落的结构影响较为明显,不同生境区的稻田节肢动物群落,都能在一定距离取值时聚合成一类,而水稻品种对此影响较小。主成分分析结果由二维图表达已基本满足要求。结果说明大八镇生境区稻田节肢动物群落与岗列镇生境区稻田节肢动物群落差异较大,分别列于两个极端。海陵镇生境区稻田介于两者之间,而与岗列镇生境区稻田节肢动物群落重叠。与聚类分析结果有所差异。这可能与主成分分析法损失部分信息有关。 相似文献
83.
The bifurcations of the periodic solutions of SEIR and SIR epidemic models with sinusoidally varying contact rate are investigated. The analysis is carried out with respect to two parameters: the mean value and the degree of seasonality of the contact rate. The corresponding portraits in the two-parameter space are obtained by means of a numerical continuation method. Codimension two bifurcations (degenerate flips and cusps) are detected, and multiple stable modes of behavior are identified in various regions of the parameter space. Finally, it is shown how the parametric portrait of the SEIR model tends to that of the SIR model when the latent period tends to zero. 相似文献
84.
The dynamic pressure method (DPM) is used for measurement of k(L)a in a 1-m(3) pilot scale fermentor in coalescing (distilled water) and noncoalescing (0.3 M Na(2)SO(4) aqueous solution) batches. The method consists in recording oxygen concentration in a batch after a small pressure change (20 kPa) in the fermentor. The upward pressure change is brought about by temporary closing and subsequent throttling of outlet gas stream and the downward change by full reopening of the gas outlet. Absorption of pure oxygen yields the same k(L)a values as absorption of air. In noncoalescing batch, the downward k(L)a values are always higher than the upward values owing to spontaneous nucleation of bubbles. The experiments performed in a stirred cell confirm this behavior. Thus, only upward pressure change should be used for measurement. The correlation of k(L)a data measured in small (18-L) and large (1000-L) vessels based on power dissipated and superficial gas velocity are in a good agreement. Unlike the DPM, the classical dynamic methods yield, under the same conditions, excessively low values of k(L)a (the dynamic startup method) or fail to produce data at all (the dynamic method with interchange of air for N(2)). (c) 1994 John Wiley & Sons, Inc. 相似文献
85.
How to sample alignments from their posterior probability distribution given two strings is shown. This is extended to sampling alignments of more than two strings. The result is first applied to the estimation of the edges of a given evolutionary tree over several strings. Second, when used in conjunction with simulated annealing, it gives a stochastic search method for an optimal multiple alignment.Correspondence to: L. Allison 相似文献
86.
87.
Fourteen lichens, 10 green algal lichens and four cyanolichens, as well as a cyanobacterium emitted significant quantities of H2S (0.01–0.04 pmol g dw–1 s–1) and DMS (0.005–0.025 pmol g dw–1 s–1) but were sinks for COS (0.015–0.14 pmol g dw–1 s–1). In contrast, exchange of CH3SH and CS2 were sporatic and inconsistent. Although some interspecific variation occurred for the first three gases, exchange rates were relatively uniform and were not influenced by irradiance conditions. In contrast to DMS and H2S emission, COS uptake was strongly influenced by degree of thallus hydration. Because lichen dominated systems cover extensive terrestrial habitats, COS uptake is potentially important in the world's sulfur budget. 相似文献
88.
Frederick C. Wedler Michael C. Vichnin Brenda W. Ley Georges Tholey Marc Ledig Jean-Christoph Copin 《Neurochemical research》1994,19(2):145-151
Previous studies have demonstrated that in glia and astrocytes Mn(II) is distributed with ca. 30–40% in the cytoplasm, 60–70% in mitochondria. Ca(II) ions were observed to alter both the flux rates and distribution of Mn(II) ions in primary cultues of chick glia and rat astrocytes. External (influxing) Ca(II) ions had the greatest effect on Mn(II) uptake and efflux, compared to internal (effluxing) or internal-external equilibrated Ca(II) ions. External (influxing) Ca(II) ions inhibited the net rate and extent of Mn(II) uptake but enhanced Mn(II) efflux from mitochondria. These observations differ from Ca(II)–Mn(II) effects previously reported with brain (neuronal) mitochondria. Overall, increased cytoplasmic Ca(II) acts to block Mn(II) uptake and enhance Mn(II) release by mitochondria, which serve to increase the cytoplasmic concentration of free Mn(II). A hypothesis is presented involving external L-glutamate acting through membrane receptors to mobilize cell Ca(II), which in turn causes mitochondrial Mn(II) to be released. Because the concentration of free cytoplasmic Mn(II) is poised near the Kd for Mn(II) with glutamine synthetase, a slight increase in cytoplasmic Mn(II) will directly enhance the activity of glutamine synthetase, which catalyzes removal of neurotoxic glutamate and ammonia. 相似文献
89.
The present paper illustrates the development of an advanced technique in optical resolution. Both of the amphetamine enantiomers can be obtained by a two-step distillation in nearly quantitative yield without any loss of the resolving agent. It is proved that the second-order interactions (H-bond) are sufficient for separation of enantiomers by distillation. © 1994 Wiley-Liss, Inc. 相似文献
90.
热带林茎流收集及计算方法探索 总被引:5,自引:0,他引:5
热带林茎流收集及计算方法探索周光益,吴仲民,李意德,陈步峰(中国林科院热带林业研究所,广州510520)CollectionandCalculationMethodsforStemflowinTropicalForest.¥ZhouGuangyi;W... 相似文献