全文获取类型
收费全文 | 1920篇 |
免费 | 225篇 |
国内免费 | 131篇 |
出版年
2024年 | 5篇 |
2023年 | 54篇 |
2022年 | 49篇 |
2021年 | 53篇 |
2020年 | 82篇 |
2019年 | 95篇 |
2018年 | 103篇 |
2017年 | 84篇 |
2016年 | 91篇 |
2015年 | 76篇 |
2014年 | 110篇 |
2013年 | 209篇 |
2012年 | 100篇 |
2011年 | 81篇 |
2010年 | 66篇 |
2009年 | 105篇 |
2008年 | 100篇 |
2007年 | 134篇 |
2006年 | 99篇 |
2005年 | 89篇 |
2004年 | 79篇 |
2003年 | 63篇 |
2002年 | 41篇 |
2001年 | 34篇 |
2000年 | 30篇 |
1999年 | 33篇 |
1998年 | 23篇 |
1997年 | 26篇 |
1996年 | 21篇 |
1995年 | 16篇 |
1994年 | 15篇 |
1993年 | 12篇 |
1992年 | 10篇 |
1991年 | 6篇 |
1990年 | 4篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 6篇 |
1986年 | 10篇 |
1985年 | 10篇 |
1984年 | 6篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1973年 | 3篇 |
1971年 | 1篇 |
排序方式: 共有2276条查询结果,搜索用时 281 毫秒
61.
A set of grid type knowledge‐based energy functions is introduced for ?–χ1, ψ–χ1, ?–ψ, and χ1–χ2 torsion angle combinations. Boltzmann distribution is assumed for the torsion angle populations from protein X‐ray structures, and the functions are named as statistical torsion angle potential energy functions. The grid points around periodic boundaries are duplicated to force periodicity, and the remedy relieves the derivative discontinuity problem. The devised functions rapidly improve the quality of model structures. The potential bias in the functions and the usefulness of additional secondary structure information are also investigated. The proposed guiding functions are expected to facilitate protein structure modeling, such as protein structure prediction, protein design, and structure refinement. Proteins 2013. Proteins 2013; 81:1156–1165. © 2013 Wiley Periodicals, Inc. 相似文献
62.
The "Blue Moon" ensemble is a computationally efficient molecular dynamics method to estimate the rate constants of rare activated events when the process can be described by a reaction coordinate ξ(r), a well-defined function in configuration space. By means of holonomic constraints a number of values of ξ(r) can be prescribed along the relevant path to identify the "bottleneck" region first and to sample an ensemble of starting conditions to generate activated trajectories. These MD trajectories sample phase space according to a biased configurational distribution. With a suitable re-weighting of averages from such ensemble of trajectories one can characterize completely rare events. 相似文献
63.
Jean-Numa Gillet 《Journal of biomolecular structure & dynamics》2013,31(4):690-705
Cell milking is a 100% renewable green energy for CO2 by extraction of biofuels inside the cytosol of photosynthetic micro-organisms as microalgae and bacteria. The cells are exposed to a hydrophobic solvent forming holes and cracks through their membranes from which the biofuels can leak out. In protein folding, the goal would be to find pathways to the unique functional protein conformer. However, in the lipid-bilayer interaction with the solvent for milking, the objective is to block the pathways for damaged membrane conformations of low free energy with undesired nanostructures, using the solvent properties, as shown with an ab initio structural bioinformatic model. Statistical thermodynamics is used to compute the free energy (including entropy) from the molecular dynamics trajectory of the biomolecular system with many conformational changes. This model can be extended to the general problem of biomolecules folding as for proteins and nucleic acids. Using an adaptation of the Einstein diffusion law, the conformational change dynamics of the lipid bilayer depends on the two diffusion coefficients of the solvent: D1 before the irreversible folding transition time and the much smaller D2 thereafter. In contrast to the n-hexane and n-heptane hydrocarbons of smaller size, the residual D2?=?4.7?×?10?7?cm2/s of the n-decane solvent, with the highest partition coefficient among the three extractors, is the only to present a D2 value that is significantly below the critical threshold of 10?6?cm2/s. Therefore, the membrane would resist to long hydrocarbons and the exposed cells would remain viable for milking. 相似文献
64.
Christina B. Nielsen Sanjay K. Singh Jesper Wengel Jens Peter Jacobsen 《Journal of biomolecular structure & dynamics》2013,31(2):175-191
Abstract LNA (Locked Nucleic Acids) is a novel oligonucleotide analogue containing a conformationally restricted nucleotide with a 2′-0, 4′-C-methylene bridge that induces unprecedented thermal affinities when mixed with complementary single stranded DNA and RNA. We have used two-dimensional'H NMR spectroscopy obtained at 750 and 500 MHz to determine a high resolution solution structure of an LNA oligonucleotide hybridized to the complementary DNA strand. The determination of the structure was based on a complete relaxation matrix analysis of the NOESY cross peaks followed by restrained molecular dynamics calculations. Forty final structures were generated for the duplex from A-type and B-type dsDNA starting structures. The root-mean-square deviation (RMSD) of the coordinates for the forty structures of the complex was 0.32Å. The structures were analysed by use of calculated helix parameters. This showed that the values for rise and buckle in the LNA duplex is markedly different from canonical B-DNA at the modification site. A value of twist similar to A-DNA is also observed at the modification site. The overall length of the helix which is 27.3Å. The average twist over the sequence are 35.9° ± 0.3°. Consequently, the modification does not cause the helix to unwind. The bis-intercalation of the thiazole orange dye TOTO to the LNA duplex was also investigated by 1H NMR spectroscopy to sense the structural change from the unmodified oligonucleotide. We observed that the bis-intercalation of TOTO is much less favourable in the 5′-CTLAG-3′ site than in the unmodified 5′-CTLAG-3′ site. This was related to the change in the base stacking of the LNA duplex compared to the unmodified duplex. 相似文献
65.
Nimra Akram Naheed Khan Mehreen Ameen Shahmeera Mahmood Komal Shamim Marium Amin 《Chronobiology international》2013,30(9):1262-1268
ABSTRACTSeveral studies have focused on determining the effect of chronotype and learning approach on academic achievement separately indicating that morning types have an academic advantage over the evening types and so have the deep learners over the surface learners. But, surprisingly none have assessed the possible relationship between chronotype and learning approach. So, the current study aimed to evaluate this association and their individual influence on academic performance as indicated by the Cumulative Grade Point Average (CGPA) as well as the effect of their interaction on academic performance. The study included 345 undergraduate medical students who responded to reduced Morningness-Eveningness Questionnaire and Biggs Revised Two-Factor Study Process Questionnaire. Morning types indulged in deep learning while evening types in surface learning. Morning and evening types did not differ on academic performance but deep learners had better academic outcomes than their counterparts. The interaction between chronotype and learning approach was significant on determining academic achievement. Our findings gave the impression that chronotype could have an impact on academic performance not directly but indirectly through learning approaches. 相似文献
66.
Bacterial biofilms adapt and shape their structure in response to varied environmental conditions. A statistical methodology was adopted in this study to empirically investigate the influence of nutrients on biofilm structural parameters deduced from confocal scanning laser microscope images of Paracoccus sp.W1b, a denitrifying bacterium. High concentrations of succinate, Mg++, Ca++, and Mn++ were shown to enhance biofilm formation whereas higher concentration of iron decreased biofilm formation. Biofilm formed at high succinate was uneven with high surface to biovolume ratio. Higher Mg++ or Ca++ concentrations induced cohesion of biofilm cells, but contrasting biofilm architectures were detected. Biofilm with subpopulation of pillar-like protruding cells was distributed on a mosaic form of monolayer cells in medium with 10 mM Mg++. 10 mM Ca++ induced a dense confluent biofilm. Denitrification activity was significantly increased in the Mg++- and Ca++-induced biofilms. Chelator treatment of various biofilm ages indicated that divalent cations are important in the initial stages of biofilm formation. 相似文献
67.
68.
69.
Nick Drydakis 《Ethnic and racial studies》2013,36(12):2054-2074
In the current study, we analyse the effect of having a Roma background on women's wages. By utilizing data from sixteen multiethnic municipalities in which Roma live, we estimate that 66.2 per cent of the wage differential between Roma and non-Roma female workers cannot be explained by differences in observed characteristics. Prejudices against Roma women are discussed and appear to explain the wage gap found here. The occupational segregation of the Roma in low-paid jobs and employers' statistical motivations are also found to influence wages earned by Roma. This study concludes that there is a need for better implementation of existing laws, rules and regulations, which would counter the discrimination against minority women in the labour market. In addition, a better means of assessing workers' skills might contribute to the reduction of wage discrimination, and greater educational achievement would significantly boost the economic status of Roma women. 相似文献
70.
Drawing on recent cross-national surveys of the Turkish second generation, we test hypotheses of secularization and of religious vitality for Muslim minorities in Europe. Secularization predicts an inverse relationship between structural integration and religiosity, such that the Turkish second generation would be less religious with higher levels of educational attainment and intermarriage. The religious vitality hypothesis predicts the maintenance of religion in the second generation, highlighting the role of religious socialization within immigrant families and communities. Taking a comparative approach, these hypotheses are tested in the context of different national approaches to the institutionalization of Islam as a minority religion in four European capital cities: Amsterdam, Berlin, Brussels and Stockholm. Across contexts, religious socialization strongly predicts second-generation religiosity, in line with religious vitality. The secularization hypothesis finds support only among the second generation in Berlin, however, where Islam is least accommodated. 相似文献