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71.
The influence of mineral nutrient availability, light intensity and CO2 on growth and shoot:root ratio in young plants is reviewed. Special emphasis in this evaluation is given to data from laboratory experiments with small Betula pendula plants, in which the concept of steady-state nutrition has been applied.Three distinctly different dry matter allocation patterns were observed when growth was limited by the availability of mineral nutrients: 1, Root growth was favoured when N, P or S were the major growth constraints. 2, The opposite pattern obtained when K, Mg and Mn restricted growth. 3, Shortage of Ca, Fe and Zn had almost no effect on the shoot:root ratio. The light regime had no effect on dry matter allocation except at very low photon flux densities (< 6.5 mol m-2 day-1), in which a small decrease in the root fraction was observed. Shortage of CO2, on the other hand, strongly decreased root development, while an increase of the atmospheric CO2 concentration had no influence on dry matter partitioning. An increased allocation of dry matter to below-ground parts was associated with an increased amount of starch in the tissues. Depletion of the carbohydrate stores occurred under all conditions in which root development was inhibited. It is concluded that the internal balance between labile nitrogen and carbon in the root and the shoot system determines how dry matter is being partitioned in the plant. The consistency of this statement with literature data and existing models for shoot:root regulation is examined. 相似文献
72.
Plant nutrition and growth: Basic principles 总被引:2,自引:0,他引:2
Soil compaction may restrict shoot growth of sugar beet plants. Roots, however, are the plant organs directly exposed to soil compaction and should therefore be primarily affected. The aim of this study was to determine the influence of mechanical resistance and aeration of compacted soil on root and shoot growth and on phosphorus supply of sugar beet. For this purpose, a silt loam soil was adjusted to bulk densities of 1.30, 1.50 and 1.65 g cm–3 and water tensions of 300 and 60 hPa. Sugar beet was grown in a growth chamber under constant climatic conditions for 4 weeks. Both, decrease of water tension and increase of bulk density impeded root and shoot growth. In contrast, the P supply of the plants was differently affected. At the same air-filled pore volume, the P concentration of the shoots was reduced by a decrease of soil water tension, but not by an increase of bulk density. Both factors also reduced root length and root hair formation, however, in compacted soil the plants partly substituted for the reduction of root size by increasing the P uptake efficiency per unit of root. Shoot growth decreased when root growth was restricted. Both characteristics were closely related irrespective of the cause of root growth limitation by either compaction or water saturation. It is therefore concluded that shoot growth in both the compacted and the wet soil was regulated by root growth. The main factor impeding root growth in compacted soil was penetration resistance, not soil aeration.FAX no corresponding author: +49551 5056299 相似文献
73.
A cDNA clone encoding Brassica calmodulin 总被引:2,自引:0,他引:2
A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino acid sequence of Brassica CaM with that of Arabidopsis CaM ACaM-2 and ACaM-3 showed 100% homology, which is not unusual, since both plants belong to the family Cruciferae. In situ hybridisation studies on Brassica seedlings using a digoxigenin-labelled RNA probe showed that high levels of CaM mRNA were detected in the leaf primordia and the shoot apical meristem, and to a lesser degree, in the zone of root elongation of the root tip. The occurrence of a higher rate of cell division and growth in these regions than its surrounding tissue may possibly be related to higher levels of CaM mRNA. 相似文献
74.
Therese Mandel Andrew J. Fleming Rolf Krähenbühl Cris Kuhlemeier 《Plant molecular biology》1995,29(5):995-1004
The NeIF-4A10 gene belongs to a family of at least ten genes, all of which encode closely related isoforms of translation initiation factor 4A. The promoter region of NeIF-4A10 was sequenced, and four mRNA 5 ends were determined. Deletions containing 2750, 689 and 188 bp of untranscribed upstream DNA were fused to the GUS reporter gene and introduced into transgenic tobacco. The three constructs mediated GUS expression in all cells of the leaf, stem and shoot apical meristem. Control experiments using in situ hybridization and tissue printing indicated that the observed GUS expression matches the expression patterns of NeIF-4A mRNA and protein. This detailed analysis at the level of mRNA, protein and reporter gene expression shows that NeIF-4A10 is an ideal constitutively expressed control gene. We argue that inclusion of such a control gene in experiments dealing with specifically expressed genes is in many cases essential for the correct interpretation of observed expression patterns. 相似文献
75.
Branka Kolevska-Pletikapić Zinka Buturović-Derić 《Plant Cell, Tissue and Organ Culture》1995,41(2):189-192
Picea omorika plants were regenerated from embryo and seedling shoot tip cultures. Adventitious and axillary shoots were produced on 1/2 MS medium containing benzyladenine and kinetin. Benzyladenine was more effective in bud induction, whereas kinetin hastened shoot development. Excised shoots were elongated on 1/3 MS medium without growth regulators, multiplied with kinetin and rooted with or without indole-3-butyric acid.Abbreviations BA
N6-benzyladenine
- 2IP
N
6-(2-isopenteny) adenine
- NAA
-naphthaleneacetic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid 相似文献
76.
Bittersweet (Solanum dulcamara L., Solanaceae) is of interest as a source of steroidal alkaloids for the commercial production of hormones. Since glycoalkaloid production is positively correlated to differentiation, tumor and teratoma cultures of the soladulcidine chemotype were established by transformation withAgrobacterium tumefaciens. A newly developed HPLC-system, which allowed separation and sensitive quantitation of the glycoalkaloids soladulcidine-tetraoside, solamargine and solasonine, was used to analyse glycoalkaloid profiles in plants and cultures. Tumors and teratoma were charcterized by a shift in their alkaloid pattern from soladulcidine tetraoside to the solasodine glycosides solamargine and solasonine. Shoot teratoma showed a total glycoalkaloid content of 1% dw, which is about fivefold higher than in the source plant. A regenerated plant retained the altered alkaloid spectrum; the levels, however, equalled those of the source plant. From the alteration of alkaloid pattern in the transformed cultures suggestions can be made concerning the biosynthetic pathway. Completion of the biosynthesis of the aglycone is likely to be complete before glycosylation occurs. 相似文献
77.
Masaru Nakano Keizo Hosokawa Tomo Oomiya Saburo Yamamura 《Plant Cell, Tissue and Organ Culture》1995,41(3):221-227
A protoplast-to-plant system was developed in Gentiana using a gellan gum-embedding culture. Viable protoplasts could be routinely isolated from in vitro-grown plantlets, and they were embedded in 0.2% gellan gum-solidified B5 medium containing 2 mg l-1 NAA, 0.1 mg l-1 TDZ, 0.1 M sucrose and 0.4 M mannitol. Weekly addition of fresh liquid medium was essential for preventing cell browning. Colony growth was promoted by lowering mannitol concentration of the culture media after one month, and visible colonies were produced after 2 months of culture. Shoot regeneration from protoplast-derived calluses was stimulated by 1 to 10 mg l-1 TDZ in combination with 0.1 mg l-1 NAA. Protoplast-derived plants were recovered following rooting of the shoots in plant growth regulator-free medium and they were successfully transferred to soil.Abbreviations BA
benzylaminopurine
- FDA
fluorescein diacetate
- FW
fresh weight
- MES
2-N-morpholinoethane sulfonic acid
- NAA
-naphthaleneacetic acid
- TDZ
N-1,2,3-thiadiazol-5-yl-N-phenylurea (also called thidiazuron) 相似文献
78.
The invasion by three different Utah populations of Pratylenchus neglectus (UTI, UT2, UT3) was similar in single and interplantings of ''Lahontan'' alfalfa and ''Fairway'' crested wheatgrass at 24 ñ 3 °C. Population UT3 was more pathogenic than UT1 and UT2 on both alfalfa and crested wheatgrass. Inoculum density was positively correlated with an invasion by P. neglectus. Invasions by UT3 at all initial populations (Pi) exceeded that of UT1 and UT2 for both single and interplanted treatments. The greatest reductions in shoot and root weights of alfalfa and crested wheatgrass were at a Pi of 8 P. neglectus/cm³ soil. Pi was negatively correlated with alfalfa and crested wheatgrass shoot and root growth and nematode reproduction. The reproductive factor (Rf) for UT3 exceeded that of UT1 and UT2 in single and interplantings at all inoculum levels. There were no differences in Rfin the Utah populations in single or interplantings. A nematode invasion increased with temperature and was greatest at 30 °C. Population UT3 was more pathogenic than UT1 and UT2 and reduced shoot and root growth at all soil temperatures. Populations UT1 and UT2 reduced shoot and root growth at 20-30 °C. Soil temperature was negatively correlated with shoot and root growth and positively correlated with nematode reproduction. Reproduction of UT3 exceeded that of UT1 and UT2 at all soil temperatures. 相似文献
79.
Protoplasts derived from tissue-cultured shoot primordia of garlic (Allium sativum L.) initiated successive cell divisions within 4 days and formed small individual calli (0.2mm in diameter) after 5 weeks of culture on Gamborg's B5 medium supplemented with 0.1% casein hydrolysate, 1mg/1 1-naphthaleneacetic acid and 1mg/1 6-benzylaminopurine. Plating efficiency was roughly 5% at the density of 1x104 protoplasts/ml of medium. Adventitious buds developed from the calli during subsequent subculture on Gamborg's B5 medium supplemented with 40mg/l adenine and 10% coconut milk. When transferred to the same medium without supplements, these buds grew into shoots and rooted. The regenerated garlic plantlets were successfully transferred to the greenhouse and grew into whole plants. 相似文献
80.
Shoot growth phenology was compared for the saplings of evergreen and deciduous woody species sharing the same microsite.
Growth initiation occurred earlier in evergreens (among co-stratal species) while deciduous species completed their growth
earlier. Shoot growth rate was significantly greater (P<0.01) for deciduous trees than evergreen trees. The amount of shoot elongations and shoot diameter was also significantly
greater (P<0.01) for deciduous trees than evergreens. On the other hand, among shrubs the amount of shoot elongation and shoot diameter
was greater for evergreens but the rate of elongation and diameter was more or less similar for both. The duration of shoot
elongation and shoot diameter was significantly longer in evergreens than the deciduous species. Leaf packing (number of leaves
per shoot) was significantly more dense in evergreen trees (P<0.01) than in deciduous tree species. Leaf packing was more dense in evergreen than deciduous shrubs but the difference was
not significant. Leaf area (per individual leaf) at full expansion was significantly greater (P<0.01) in deciduous species. Leaf dry mass and specific leaf mass in the initial stage was significantly greater for evergreen
species than for deciduous species. The number of buds/10 cm of shoot was higher in evergreens. However, the per cent mortality
was also higher in them. 相似文献