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91.
Alessandro Terrinoni Carmen Di Franco Patrizio Dimitri Nikolaj Junakovic 《Journal of molecular evolution》1997,45(2):145-153
The intragenomic location of the elements of the I, G, jockey, F, and Doc transposon families has been studied by the Southern
blot analysis, in 12 laboratory Drosophila melanogaster stocks. Elements located in euchromatin, heterochromatin, and on the Y chromosome are identified, and their stability has
been assessed by comparing the autoradiographs detected in different stocks and analysis of individual flies. Evidence is
shown suggesting that preferential location in euchromatin or heterochromatin and the distribution within heterochromatin
are distinctive of transposon families. Elements located in heterochromatin can be unstable. These results are discussed in
the context of the relationship between transposable elements and the host genome.
Received: 21 August 1996 / Accepted: 24 March 1997 相似文献
92.
The sequences of the entire blue opsin gene in the squirrel monkey (Saimiri boliviensis) and the five introns of the human blue opsin gene were obtained. Intron 3 of these genes contains an Alu sequence and intron
4 contains a partial mer13 sequence. A comparison of the squirrel monkey opsin sequence with published mammalian opsin sequences
shows that features believed to be functionally critical are all conserved. However, the blue opsin has evolved twice as fast
as rhodopsin and is only as conservative as the β globin, which has evolved at the average rate of mammalian proteins. Interestingly,
the interhelical loops are, on average, actually more conservative than the transmembrane α helical regions. The introns of
the blue opsin gene have evolved at the average rate of introns in primate genes.
Received: 5 August 1996 / Accepted: 2 October 1996 相似文献
93.
94.
M. T. Rajan D. Champion C. S. Kumar D. Vishnuvardhan K. S. Jagannatha Rao M. A. Viswamitra 《Molecular biology reports》1995,22(1):47-52
Spectroscopic study on the interactions of trace elements Co, Mn, Mg and Al with d(GCGTACGC) indicated the following: Al and Mg did not alter Tm values. Mn enhanced Tm at lower concentration and decreased it at higher concentrations. Interestingly Co at higher concentration elevated the Tm. These studies also showed lower concentrations of Mn displaced EtBr, whereas Al could displace it at higher ionic strength. Mg and Co displaced EtBr fluorescence at moderate concentrations. The binding constant values and CD spectra clearly indicated strong binding of these elements to DNA. 相似文献
95.
五台山山地草甸自然保护区11种化学元素生物积累的研究 总被引:5,自引:0,他引:5
五台山山地草甸自然保护区可划分为亚高山草甸带、山地五花草甸带、常绿针叶林草甸带。本文研究了各生态带土壤和植被中11种化学元素的含量、分布及生物积累特征。研究表明:(1)各带土壤中化学元素含量均在全国同类型土壤含量范围之内,就平均含量而言,Co'Ni含量较高,其它元素含量较低,整体属清洁理想水平,合乎自然保护区土壤环境标准。(2)各带植被中营养元素含量较高,营养丰富。尤其是亚高山草甸带是饲用价值很高 相似文献
96.
Leentje Goosen-de Roo R. Bakhuizen Paulina C. van Spronsen K. R. Libbenga 《Protoplasma》1984,122(1-2):145-152
Summary Fusiform cambial cells of the ash (Fraxinus excelsior L.), which are strongly elongated and vacuolated, contain a phragmosome which traverses the whole length of the cells during preprophase and karyokinesis and which remains present during cytokinesis until it is integrated in cell plate with adjacent cytoplasm.The phragmosome consists of a thin perforated cytoplasmic layer located in the plane of the future cell plate. Otherwise oriented transvacuolar cytoplasmic layers or strands are not present in these cells.The phragmosome contains cytoskeletal elements, namely microtubules and also microfilament bundles both of which are oriented mainly in longitudinal direction.The phragmosomal microtubules are a new category of microtubules associated with cell division; presumably they guide the centrifugally growing cell plate to the parental cell wall site previously marked by the preprophase band of microtubules. 相似文献
97.
We have determined the complete nucleotide sequence of the right (R) copy of the insertion sequence IS15 which flanks, in direct orientation, the composite transposon Tn1525. IS15-R, which is capable of independent transposition, is 1648 bp long and has short (14 bp) perfect inverted repeats at its termini. Analysis of the nucleotide sequence indicates that IS15-R results from the transposition, in direct orientation, of a smaller (820 bp long) IS, designated IS15-Δ, into itself. This integration event is accompanied by the duplication of 8 bp in the target DNA. IS15-Δ possesses two large overlapping open reading frames (ORF) located on opposite strands. Because of this particular structure, IS15 possesses four large ORFs which, due to the integration event, exhibit some differences with those of the parental 1S15-Δ. 相似文献
98.
99.
Damian Labuda Daniel Sinnett Chantal Richer Jean-Marc Deragon George Striker 《Journal of molecular evolution》1991,32(5):405-414
Summary In a recent report mouse B1 genomic repeats were divided into six families representing different waves of fixation of B1 variants, consistent with the retroposition model of human Alu elements. These data are used to examine the distribution of nucleotide substitutions in individual genomic repeats with respect to family consensus sequences and to compare the minimal energy structures of the corresponding B1 RNAs. By an enzymatic approach the predicted structure of B1 RNAs is experimentally confirmed using as a model sequence an RNA of a young B1 family member transcribed in vitro by T7 RNA polymerase. B1 RNA preserves folding domains of the Alu fragment of 7SL RNA, its progenitor molecule. Our results reveal similarities among 7SL-like retroposons, human Alu, and rodent B1 repeats, and relate the evolutionary conservation of B1 family consensus sequences to selection at the RNA level. 相似文献
100.