乙型脑炎病毒NS2A-C60A位点突变对其生物学特性影响研究*

目的:旨在探索Ⅰ型日本乙型脑炎病毒传代致弱后基因组突变NS2A-C60A对乙脑病毒生物学特性的影响。方法:首先通过对传代致弱及原始乙脑毒株基因组序列进行测序比对、结构预测分析并利用Western blotting(WB)确定了目标研究位点NS2A-C60A;然后使用反向遗传定点突变技术构建拯救了包含NS2A-C60A单点突变的病毒株;最后利用噬斑形态观察、生长曲线、双萤光素酶分析,WB以及炎性因子检测和动物实验研究了该单点突变对于乙脑病毒生物学特性的影响。结果:首次研究发现Ⅰ型乙脑病毒传代致弱会导致NS1'蛋白表达的显著下降以及可能的相关位点NS2A-C60A,并成功拯救获得了NS2A-C60A单点突变毒株rJEV-C60A,研究发现NS2A-C60A突变对乙脑病毒的生长特性及噬斑形成没有显著影响,但是能够显著降低乙脑病毒NS1'蛋白的表达,并且该位点突变能够轻微阻碍乙脑病毒对细胞炎性因子表达的抑制,动物实验结果显示NS2A-C60A点突变病毒与原毒株具有相似的神经毒力,说明该位点突变不是影响乙脑病毒毒力致弱的关键位点。结论:新发现的NS2A-C60A位点突变能够显著减少乙脑病毒NS1'蛋白的表达,但是对其增殖、诱导炎症及神经毒力等生物学特性没有显著影响。     

离散广义Logistic模型的稳定性和浑沌现象

文[1]提出了单种生长的连续性文广义Logistic dx/dt=γx({K-x}/(K+px))其中γ>ο为种群的内禀生长率,K>0为环境容纳量,ν>-1表示种群对环境(包括营     

Progressive changes in the structure and dynamics of the phytoplankton community along a pollution gradient in a lowland river — a multivariate approach

The phytoplankton of the River Lujan (Buenos Aires, Argentina) was studied for a period of 18 months, together with physical and chemical variables, in relation to a pollution gradient. 167 taxa were recorded within a seasonal succession characterized by dominance of diatoms with a brief summer green algae facies. A combination of several biotic indices and multivariate analysis was employed to assess the impact of pollution on the phytoplankton community. The biotic indices used were species diversity and richness, algal quotients (green algae/diatom ratio, Centrales/Pennales ratio) and the SD succession rate index. Multivariate procedures included cluster analysis and ordination by PCA of both species and samples, stepwise discriminant analysis and multiple discriminant analysis of variance (MANOVA). Results indicate that community dynamism is attenuated at the more polluted sites, concomitant with an increased predominance of a broad-tolerance algal assemblage, co-dominated by Cyclotella meneghiniana and Nitzschia stagnorum. The changes in the community structure and dynamics described herein involved alterations in the distribution and relative proportions of the algae, rather than modifications in the basic species composition. These changes may not be readily detectable by methods which over-simplify the ecological information, such as systems of indicator species and biotic indices, designed to assess the degree of pollution. The suitability of multivariate analysis and biotic indices in river phytoplankton studies is further discussed.     

Heavy metals (Cu and Zn) in recent sediments of Llangorse Lake,Wales: non-ferrous smelting,Napoleon and the price of wheat — a palaeoecological study

Elevated concentrations of Cu and Zn have been found in the upper part of three sediment cores collected from Llangorse Lake, in south Wales. Palaeomagnetic evidence from one of the cores and ²¹⁰Pb analysis of another, suggests that the increase in sediment Cu and Zn concentrations began during the eighteenth century. A sharp increase in the concentrations of these metals in the sediment profile appears to have occurred during the latter part of the eighteenth century and these concentrations remained high until the mid to late nineteenth century.The absence of known ore deposits and industry around the lake suggests that the lake and catchment soils were increasingly contaminated by long-range aerial transport of emissions from the expanding activity of Cu and Zn smelters located some 80 km upwind in the Swansea area during the Industrial Revolution. Evidence from agricultural crop returns indicates a significant increase in the amount of land devoted to tillage in the catchment, particularly to cereal production, during the late eighteenth and the first half of the nineteenth century which included the Napoleonic Wars. This agricultural shift appears to coincide with increased concentrations of Cu and Zn in the lake sediments. It is suggested that newly ploughed soils, contaminated with metals for many years by long-range aerial transport from the Swansea area, eroded, and were carried into the lake by catchment run-off and added to the sediment burden of Cu and Zn. A subsequent decline of Cu and Zn emissions due to the collapse of the non-ferrous smelting industry and reduced soil erosion because of a 50% reduction of tillage due to an agricultural depression in the second half of the 19th century may explain the fall in Cu and Zn concentrations in the upper part of the sediment profile. The most recent sediments (20th century) show the increase in heavy metals characteristic of many lakes around the world.     

Macrobenthic community structure before and after pollution abatement in the Neches River estuary (Texas)

Macrobenthos and physicochemical conditions in the lower 39 km of the Neches River estuary were studied from August, 1984 to May, 1985. The results were compared with data collected in 1971–1972. Between 1972 and 1984 the permitted BOD waste load in the tidal Neches River was reduced from 123 125 kg d to 8717 kg d. River discharge and dissolved oxygen concentrations were consistently higher and salinity was lower, during the same seasons, during the 1984–1985 study. A total of 50 taxa of macrobenthos were collected in 1971–1972 and 104 taxa were collected in 1984–1985. The numbers of taxa per collection at each station in 1984–1985 were at least twice those found in 1971–1972. Minimum densities in 1984–1985 were much higher than the maximum densities in 1971–1972 at all stations. Patterns of dominance, Sorenson's similarity index, and diversity ( ) values indicated improved water quality in 1984–1985. Statistical analysis of macrobenthic diversity indicated significant differences between upper estuary and lower estuary stations in 1971–1972. No significant differences were found in 1984–1985. Significant differences in numbers of taxa, macrobenthos densities, and values between the two studies were found. Reductions of waste loads, increased river discharge, and deepening of the navigation channel were among the factors that probably contributed most to the changes in community structure of the macrobenthos observed.     

几种生态因子对菹草光合作用的影响

本文研究了光照、pH、温度对菹草光合作用的影响。在一定温度条件下,菹草的净产氧量与一定范围的光照强度呈直线相关。菹草的光补偿点随温度的上升而上升。在菹草自然生活的环境中,温度低于30℃时,升温有利于菹草的光合作用。高pH(PH>10.0)下碳源缺乏对菹草的光合作用影响较大。高pH与强光照射的协同作用严重影响菹草的光合作用。水温与氮、磷营养盐不足并非夏季自然水体中菹草死亡的主要原因。而不良光照(水表层光抑制,中、下层光饥饿)和高pH下缺乏光合碳源的协同作用便可能导致菹草夏季死亡。     

土壤重金属污染的酶学诊断:紫色土Cd、Cu、Pb和As对水稻根系脱氢酶的影响

本文通过酸性紫色土和石灰性紫色土中不同浓度Cd、Cu、Pb、As对水稻根系脱氢酶的影响研究,揭示了脱氢酶受抑制与产生抗性的过程。在低浓度时,土壤Cd、Cu、Pb、As对脱氢酶的影响较敏感,能因元素的不同性质反映土壤类型影响的差别。最后,以脱氢酶受抑制与初始抗性峰出现的转折点相应的土壤浓度为依据,确定了两种紫色土Cd、Cu、Pb、As的临界浓度。     

羊红细胞超氧化物歧化酶的研究

自羊红细胞分离得到一种高等电点的铜.锌-超氧化物歧化酶(Cu.ZnSOD)。其沉降系数(S)为3.23,亚基分子量为16600,等电点为8.50,紫外最大吸收峰位于259nm,酶分子中含有铜和锌,氨基酸组成特点与其它动物来源的Cu.Zn-SOD相同。该酶的比活性为5500U/mg(黄嘌吟氧化酶—细胞色素还原法);对KCN的抑制作用敏感,最适pH值为6。     

Aspects of seagrass ecology along the western Arabian Gulf coast

Results of semi-quantitative observations and quantitative sampling of seagrasses at coastal and offshore sites along the western Arabian Gulf are presented. Overall seagrass cover (all species together) shows significant positive correlation with latitude, but not with salinity, temperature or depth. The same pattern is shown by Halodule uninervis (Forsk.) Aschers., the dominant species. Mean seagrass biomass ranged from 53–235 g m^-2 (dry weight). These values are comparable with biomass estimates from regions in which environmental conditions are generally less extreme than in the Gulf. Seagrass biomass is significantly negatively correlated with depth and sediment grain size. No significant correlation is apparent between seagrass biomass and factors such as season, salinity, or concentrations of nutrients and heavy metals measured. It is pointed out that any correlations observed are not necessarily taken to imply causality.     

Effects of paper mill effluents on the fish fauna of stony shores of Lake Päijänne

The fish fauna of the stony littoral in the central parts of L. Päijänne was studied by electric fishing on four occasions during 1988–1989. Ten fish species and 1681 individuals (14.5 kg) were caught in the 15 fishing sites (4096 m²) which gives a mean density of 0.41 ind. m^–2 and biomass of 3.5 gm^–2. Effluent from two large paper mills causes a clear zonation of the fish fauna in the area. In the most polluted shores, only burbot and perch occurred regularly and the reproduction of other species was inhibited. In the semipolluted area (5–15 km from the Kaipola paper mill), burbot and stone loach occurred regularly, but owing to low numbers of perch and bullhead the total densities were usually low.The clean shores were characterized by an abundance of stone loach and bullhead and locally also of minnow, which seemed to be very sensitive to pollution. The strong floods during summer 1988 had positive effects on the reproduction of perch and pike, but the densities of other species were highest during the normal water level in autumn 1989.