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91.
The symptoms of the leaf scald disease can be reproduced in vitro through the inoculation of sugarcane tissue culture plantlets. The pathogen is detected in the inoculated plantlet and is maintained at the surface of the base of the plantlets grown in vitro. Two strains of X. albilineans belonging to different serovars and lysovars reacted like pathotypes. The importance of the plant incubation temperature is clearly demonstrated. Further, in vitro the disease goes through the same phase of latency as in the field. 相似文献
92.
Yasumasa Kunifuji Terumasa Nakamura Masayuki Takasugi 《Biological trace element research》1987,14(3):237-248
Cd induced changes of Zn and Cd distribution in the liver and kidneys were studied in relation to Cd metallothionein (MT)
synthesis. Wistar male rats were given CdCl2 by sc injection of .8, 1.5, and 3.0 mg Cd/kg three times a week for three weeks. Cd levels of liver and kidneys increased
with the increment of Cd dosage and 80–90% of Cd was found in the cytosol. The MT fractions contained 80–89% cytosolic Cd
in the liver and 55–75% Cd in the kidneys. Zn concentrations in the liver increased following Cd administration, But Zn in
the kidneys showed only slight increase. There was a distinct decrease of Cu concentration in the liver of the 3.0 mg group.
In contrast, Cu concentrations in the kidneys increased about three times in the .8 and 1.5 mg Cd groups, but Cu in the 3.0
mg group showed only 1.5 times increase. The changes of these metal concentrations were observed mainly in the cytosol. Non-MT-Cd
in the kidneys was maximum in the 1.5 mg group, but the 3.0 mg group showed significant decrease. In parallel with this decrease
of Cd, Cu and Zn in the kidneys showed similar decrease. When the kidneys are injured, Zn and Cu appear to leak from this
organ. 相似文献
93.
EFFECT OF MEDIA ON GROWTH AND INTERACTIONS BETWEEN A RANGE OF SOIL-BORNE GLASSHOUSE PATHOGENS AND ANTAGONISTIC FUNGI 总被引:10,自引:1,他引:9
JOHN M. WHIPPS 《The New phytologist》1987,107(1):127-142
94.
Phytochrome, activated by continuous red light, increases the amount of total polyadenylated RNA during photomorphogenesis of mustard (Sinapis alba L.) cotyledons. In-vitro translation of total polyadenylated RNA in a reticulocyte translation system has shown that the activity of translatable -amylase mRNA is increased by phytochrome about threefold in the 3-d-old cotyledons, based on equal amounts of polyadenylated RNA, and about eightfold on a per-cotyledon basis. Cordycepin prevents the accumulation of translatable -amylase mRNA. It is concluded that the phytochrome-mediated control of -amylase synthesis is exerted on the level of mRNA synthesis. During seedling development in continuous red light, a phytochrome-dependent increase of -amylase mRNA can be observed at least 6 h before the onset of -amylase synthesis. If, after a period of enzyme synthesis, phytochrome action is interrupted by long-wavelength far-red light followed by darkness, -amylase mRNA as well as -amylase synthesis remain at a high level for 8–10 h and then decline sharply. It is concluded that -amylase mRNA, having an apparent lifetime of the order of 8–10 h, can be formed under the influence of phytochrome during early seedling development but it activates -amylase synthesis only after a lag-phase of about 8 h, when the cotyledons acquire competence to synthesize the enzyme. The consequences of these findings for the signal-transduction chain of phytochrome are discussed.Abbreviations EDTA
Na2-ethylenediaminotetraacetic acid
- PAGE
polyacrylamide gel electrophoresis
- poly(A)+RNA
polyadenylated mRNA
- Pr, Pfr
red- and far-red-absorbing forms of phytochrome
- SDS
sodium dodecyl sulfate
- Tris
2-amino-2-(hydroxymethyl)-1,3-propanediol 相似文献
95.
When 2,4-dichlorophenoxyacetic acid (2,4-D)-dependent tobacco cell suspensions, one normal and one transformed by Agrobacterium tumefaciens, were subcultured on hormone-lacking medium the stationary phase of the cell cycle was reached earlier than on medium containing 2,4-D. Addition of the auxin 2,4-D could restore cell division activity within 10–12 h for the most rapidly reacting cell line. The cell-division response was characterized as being auxin-specific and optimal with 2,4-D at 2.2 10-6 M. Although the cell lines used showed different characteristics, both reacted with a rapid increase in at least three mRNA species within 1 or 2 h after 2,4-D application. Two, 2,4-D-induced protein spots, seen after in-vitro translation, had the same characteristics (MWs 35 kilodaltons (kDa) and 25 kDa with isoelectric points of 7.1 and 6.3, respectively) in both cell lines. Water-treated controls did not show alterations in the translatable mRNA populations. This indicates that the accumulation of the corresponding mRNAs is an early hormone-induced event. Since cell division is the only measurable reaction found after auxin application, cell systems as described here offer excellent possibilities for studying early auxin-induced changes at the molecular level preceding mitosis.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- kDa
kilodalton 相似文献
96.
P. A. Scolnik P. Hinton I. M. Greenblatt G. Giuliano M. R. Delanoy D. L. Spector D. Pollock 《Planta》1987,171(1):11-18
The tomato (Lycopersicon esculentum (L.) Mill.) ghost plant is a mutant of the San Marzano cultivar affected in carotenoid biosynthesis. ghost plants exhibit a variable pattern of pigment biosynthesis during development. Cotyledons are green but true leaves are white. Green sectors, which appear to be clonal in origin, are frequently observed in the white tissue. Because of the lack of photosynthesis ghost plants have a very low viability in soil. We have developed a strategy for propagating ghost plants that employs organ culture to generate variegated green-white plants which, supported by the photosynthetic green areas, develop in soil to almost wild-type size. These plants were used to analyze the pigment content of the different tissues observed during development and plastid ultrastructure. Cotyledons and green leaves contain both colored carotenoids and chlorophyll but only the colorless carotenoid phytoene accumulates in white leaves. the plastids in the white tissue of ghost leaves lack internal membrane structures but normal chloroplasts can be observed in the green areas. The chromoplasts of white fruits are also impaired in their ability to form thylakoid membranes. 相似文献
97.
M. Buiatti C. Simeti S. Vannini G. Marcheschi A. Scala P. Bettini P. Bogani M. G. Pellegrini 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,75(1):37-40
Summary To obtain Tomato cell lines with an altered capacity to respond to heat-released cell wall components (elicitor) of a tomato pathogen (Fusarium oxysporum f. sp. lycopersici), positive and negative selection experiments, using BUdR enrichment techniques, were carried out on suspension cultures of the susceptible, low phytoalexin producer cultivar Red River. Both high and low phytoalexin producing clones were isolated. Further tests demonstrated that not all phytoalexin-producing clones were more susceptible to the elicitor toxic effect, and that they were altered also in the speed of response to fungal cell wall components. Cells selected with Fusarium elicitor showed the same behaviour when challenged by Phytophthora infestans elicitor, thus suggesting in this case lack of specificity. The results are finally discussed with a view to using the technique both as a tool to study the genetics and physiology of hostparasite interactions and as a possible new method for the selection of pathogen resistant genotypes.Paper no. 1224 IPRA-CNR; research supported by an EEC-BAP contract 相似文献
98.
The mean dimensions of thecis N-methyl peptide unit have been arrived at by analysing the crystal structure data on compounds containing such units. These
dimensions can be used as standard in conformational studies on cyclic peptides. While the bonds meeting at C are almost coplanar,
those meeting at N show a slight pyramidal disposition. A comparison of the dimensions of the normal and N-methylatedcis peptide units show that there are perceptible differences in the parameters connected with N. In addition, the flexibility
of thecis peptide unit has been analysed by studying the distribution of the parameters in different classes of compounds such as cyclic
di, tri and higher peptides. The salient features are: (i) The angle CαCN in cyclic dipeptide and the angle CδNCα in higher peptides tend to be lower, when the peptide unit is associated with a prolyl residue; (ii) in cyclic tripeptides
the internal anglesviz., CαCN and CNCα are significantly larger thereby increasing the intra-annular space; (iii) the bond Cα-C is distinctly shorter when it occurs in cyclic dipeptides. The results lead to the conclusion that thecis peptide unit takes up aneed-based flexibility in its dimension. 相似文献
99.
S. K. Podder A. Chakraborti K. Vijayalakshmi P. Lalit Kumar Singh 《Journal of biosciences》1987,11(1-4):495-502
To examine how surface Potential controls the reactivity of glycoconjugates at cell surface, the interaction of galactose-sPecific
lectinse.g. peanut agglutinin,Ricinus cummunis agglutinin with liPosomes bearing asialo GM1 were studied in the Presence of varying amount of ganglioside mixture, GMn. The Presence of 5% GMn causes comPlete slowing down of PreciPitin reaction and thereby make carbohydrate moiety of asialo GM1 comPletely inaccessiblei.e. ‘cryPtic’. In contrast the Presence of 1–2% GMn enhances the aPParent rate and amPlitude of the PreciPitin reaction as surface Potential becomes more negative. The relevance
of the findings has been discussed in relation to the exPression and involvement of the cell-surface sialic acid residues
during develoPment and differentiation. 相似文献
100.
Environmental factors influencing the occurrence of juvenile fish in the mangroves of Pagbilao,Philippines 总被引:2,自引:1,他引:1
Leonard Pinto 《Hydrobiologia》1987,150(3):283-301
128 fish species belonging to 54 families were collected from the mangroves of Pagbilao. Ambassis kopsi (Ambassidae) was the most abundant species.Fourteen environmental factors were correlated to the catch per unit time for some species. The occurrence of A. kopsi was positively correlated to nitrate in the water, carbon in the sediments and litter fall. Out of the 8 species investigated, 6 were positively correlated to litter fall. Three species showed positive correlation to phosphate in the water, two to organic carbon in the sediments, nitrate, silicate and pH, and one to salinity and carotenoids in water. The biomass of the total catch was correlated to carbon in the sediments and litter fall. 相似文献