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81.
C. EDUARDO VALLEJOS 《Plant, cell & environment》1991,14(1):105-112
Abstract. Comparisons were made between the changes in mRNA levels induced by low night temperatures in the cold–sensitive tomato and two altitudinal ecotypes of the wild species L. hirsutum. Changes in mRNA levels were detected by resolving in vitro translation products of poly(A)+ RNA by 2-D PAGE. The treatment was applied by first growing plants in a thermoperiod of 25/18°C and then switching to 25/6°C. All tomatoes displayed a diurnal cycling in which a set of mRNAs accumulated at the end of the 18°C nights, whereas another accumulated at the end of the 25°C days. The accumulation of night specific mRNAs was inhibited by 6°C nights in the cold sensitive tomatoes while that of the tolerant one was only marginally affected. All tomatoes showed a similar reduction in the apparent turnover rate of the day specific mRNAs during the 6°C nights. Finally, low night temperatures induced the accumulation of six to eight mRNAs in all genotypes. This number increased by 15 in L. esculentum after the seventh night and are likely involved in stress response rather than acclimation/tolerance. The tomato is proposed as a genetic model to discriminate genes involved in acclimation/tolerance from those involved in stress response. 相似文献
82.
Ian D. Hodkinson Jeremy M. Bird Jane K. Hill Robert Baxter . 《Ecological Entomology》2001,26(4):376-387
1. Salix lapponum host plants at an upper altitudinal site differed significantly in size, structural density, phenology, growth performance, and spatial isolation from those growing at a lower site. 2. Plant differences were paralleled by significant differences in psyllid population density and phenology parameters, with psyllid population density, percentage of catkins occupied, and phenological development relatively lower or retarded at the upper site. Population densities at the upper site, nevertheless, remained high. 3. Plant measurements were good predictors of insect density, often explaining up to 73% of the variance in abundance among plants at a given site. 4. Sets of four plant characters identified by best subsets regression were better predictors of psyllid density and development than single factors, although differences were often not great and the combinations of characters selected by multiple regression sometimes differed from the best single predictors. 5. Best single predictors of psyllid density on catkins were measurements of plant size, particularly height, length, and basal stem diameter. Shoot density and catkin phenology were occasionally important but plant isolation and prior growth performance were less important. 6. By contrast with density, age structure of the psyllid population was predicted best from plant phenological measurements, notably catkin phenology. 相似文献
83.
84.
Microsclerotium formation by six isolates of Verticillium dahliae was studied at different temperatures both in vitro and in Arabidopsis thaliana . In vitro mycelial growth was optimal at 25°C, but microsclerotium formation was greatest at 20°C (two isolates) or 15–20°C (one isolate). Seedlings of A. thaliana were root-dipped in a conidial suspension, planted, and either placed at 5, 10, 15, or 25°C, or left at 20°C until the onset of senescence, after which some of the plants were placed at 5, 10, 15, or 25°C. The amount of microsclerotia per unit of shoot weight was assessed in relation to isolate and temperature. The optimal temperature for production of microsclerotia was 15–25°C. Two isolates each produced about 10 times more microsclerotia than each of the other four isolates. For these isolates, high R 2 adj. -values of 0.77 and 0.66 were obtained, with temperature and its square as highly significant (P < 0.001) independent variables. R 2 adj. -values for the other isolates varied between 0.28 and 0.39. Moving plants to different temperatures at the onset of senescence led to microsclerotial densities that were intermediate between densities on plants that had grown at constantly 20°C and plants grown at other temperatures. This suggests that vascular colonization rate and rate of microsclerotium formation are similarly affected by temperature. The senescence rate of plants appeared unimportant except for plants grown at 25°C, which showed the highest amounts of microsclerotia per unit of plant weight in the most rapidly senescing plants. 相似文献
85.
OKSANA P. ANDRUSHCHYSHYN KEVIN P. WILSON D. DUDLEY WILLIAMS 《Freshwater Biology》2007,52(9):1745-1761
1. Ciliated protozoans (Phylum Ciliophora) were collected from five sites in a shallow groundwater system in southern Ontario, Canada over a 13‐month period: one at the spring source, two along the channel banks, and two in the stream channel. Ciliates and environmental data were collected from surface water and at five depths into the sediment, located at 20, 40, 60, 80 and 100 cm. 2. Species richness was high (170 ciliate species belonging to 89 genera were identified) and variable, both spatially and temporally. Highest species richness (86) occurred between 20 and 60 cm, and typically decreased below 60 cm. 3. Ciliate densities were also seasonally and spatially variable. Densities peaked in March between 40 cm (as high as 69 900 cells L−1) and 60 cm, and again in May and June at 80 and 100 cm. Densities were lowest in winter. The surface‐water ciliate community had a different species composition and lower population densities. 4. At all depths, small (<50 μm) bacterivorous ciliates typically dominated, but omnivorous and predatory species were also present (combined, up to 30% of the average density). 5. Several ciliate genera, traditionally considered planktonic, occurred at low densities from 40 cm down to 100 cm. 6. Ordination analysis indicated that the main factors influencing the shallow groundwater ciliate communities were depth and temperature. 7. Dissolved oxygen also appeared to influence these communities in that they typically comprised genera that preferred either low‐oxygen or anaerobic conditions. 相似文献
86.
1. The larger lakes of the English Lake District have been the subject of intensive scientific study for more than 60 years. Year‐to‐year variations in the weather have recently been shown to have a major effect on their physical characteristics. The area is mild but very wet and the dynamics of the lakes are strongly influenced by the movement of weather systems across the Atlantic. 2. Here, we combine the results of long‐term measurements and the projections from a Regional Climate Model (RCM) to assess the potential impact of climate change on the surface temperature and residence times of the lakes. 3. The RCM outputs used were produced by the U.K. Hadley Centre and are based on the IPCC ‘A2’ scenario for the emission of greenhouse gases. These suggest that winters in the area will be very much milder and wetter by the 2050s and that there will be a pronounced reduction in the summer rainfall. 4. An analysis of the meteorological data acquired between 1940 and 2000 shows that there have been progressive increases in the winter air temperature and in the rainfall which are correlated with the long‐term change in the North Atlantic Oscillation. The trends reported during the summer were less pronounced and were correlated with the increased frequency of anticyclonic days and a decrease in the frequency of westerly days in the British Isles. 5. A simple model of the year‐to‐year variations in surface temperatures showed that the highest winter temperatures were recorded in the deeper lakes and the highest summer temperatures in the lakes with the shallowest thermoclines. When this model was used to predict the surface temperatures of the lakes in the 2050s, the greatest winter increase (+1.08 °C) was observed in the shallowest lake and the greatest summer increase (+2.18 °C) in the lake with the shallowest thermocline. 6. The model used to estimate the seasonal variation in the residence time of the lakes showed that the most pronounced variations were recorded in lakes with a short residence time. Average winter residence times ranged from a minimum of 10 days to a maximum of 436 days and average summer values from a minimum of 23 days to a maximum of 215 days. When this model was used to predict the residence time of the lakes in the 2050s, the greatest winter decrease (−20%) was observed in the smallest lake and the greatest summer increase (+92%) in the lake with the shortest residence time. 7. The results are discussed in relation to trends reported elsewhere in Europe and the impact of changes in the atmospheric circulation on the dynamics of the lakes. The most serious limnological effects were those projected for the summer and included a general increase in the stability of the lakes and a decrease in the flushing rate of the lakes with short residence times. 相似文献
87.
Aims Rhododendron ponticum L. is reputed to be a post Plio‐Pleistocene relict plant species with a disjunct distribution that comprises the Iberian Peninsula to the west and the Euxinian region plus some restricted Mediterranean areas to the east. We analysed the ecological range (of subsp. baeticum) in the western area (Aljibe Mountains, north of the Strait of Gibraltar) to understand the factors determining the present area limitation. Location Sierra del Aljibe, north of the Strait of Gibraltar (Iberian Peninsula). Methods We selected 20 riparian sites where R. ponticum is common, and compiled data on the ecological diversity of associated woody species and ferns. We established a 500‐m main transect in each site, along the stream or river course, in which we placed five 20‐m‐long plots at regular intervals. We recorded physiographic habitat features, woody plants and fern abundance, and the number of R. ponticum individuals. Results Rhododendron ponticum in southern Spain is restricted to riparian forests in acidic soils (pH 4.0–6.4), and is mainly found on the banks of inclined and enclosed streams. In our inventory we recorded 59 woody taxa and 12 ferns, with R. ponticum being the dominant species of the understorey (mean abundance 78.6%). The communities are characterized by a high incidence of the humid warm temperate element, both in number of species (18.8 ± 3.7 per site) and abundance; meanwhile, the presence of the modern Mediterranean element (mean number of species 3.4 ± 3.8 per site) appears to be favoured by disturbance. These ecological–historical groups of taxa also show distinct patterns of typological habit, frequency of endemism, infrageneric diversity and geographical range. Populations of R. ponticum are characterized by a very variable density of seedlings in many sites, and the virtual lack of juveniles. Main conclusions Riparian forests of the Aljibe Mountains constitute a refuge for R. ponticum where the species persists, but populations appear to be in decline. The narrow ecological range of R. ponticum in the area strongly contrasts with its wide amplitude in the eastern natural area, mainly the Euxinian region, where R. ponticum probably finds better conditions due to the environmental heterogeneity of the region, and the lack of a hot dry season. 相似文献
88.
Sophie Arnaud-Haond Marianna Migliaccio Elena Diaz-Almela Sara Teixeira Mirjiam Susanne van de Vliet Filipe Alberto Gabriele Procaccini Carlos M. Duarte Ester A. Serrão 《Journal of Biogeography》2007,34(6):963-976
Aim The seagrass, Posidonia oceanica is a clonal angiosperm endemic to the Mediterranean Sea. Previous studies have suggested that clonal growth is far greater than sexual recruitment and thus leads to low clonal diversity within meadows. However, recently developed microsatellite markers indicate that there are many different genotypes, and therefore many distinct clones present. The low resolution of markers used in the past limited our ability to estimate clonality and assess the individual level. New high‐resolution dinucleotide microsatellites now allow genetically distinct individuals to be identified, enabling more reliable estimation of population genetic parameters across the Mediterranean Basin. We investigated the biogeography and dispersal of P. oceanica at various spatial scales in order to assess the influence of different evolutionary factors shaping the distribution of genetic diversity in this species. Location The Mediterranean. Methods We used seven hypervariable microsatellite markers, in addition to the five previously existing markers, to describe the spatial distribution of genetic variability in 34 meadows spread throughout the Mediterranean, on the basis of an average of 35.6 (± 6.3) ramets sampled. Results At the scale of the Mediterranean Sea as a whole, a strong east–west cleavage was detected (amova) . These results are in line with those obtained using previous markers. The new results showed the presence of a putative secondary contact zone at the Siculo‐Tunisian Strait, which exhibited high allelic richness and shared alleles absent from the eastern and western basins. F statistics (pairwise θ ranges between 0.09 and 0.71) revealed high genetic structure between meadows, both at a small scale (about 2 to 200 km) and at a medium scale within the eastern and western basins, independent of geographical distance. At the intrameadow scale, significant spatial autocorrelation in six out of 15 locations revealed that dispersal can be restricted to the scale of a few metres. Main conclusions A stochastic pattern of effective migration due to low population size, turnover and seed survival is the most likely explanation for this pattern of highly restricted gene flow, despite the importance of an a priori seed dispersal potential. The east–west cleavage probably represents the outline of vicariance caused by the last Pleistocene ice age and maintained to this day by low gene flow. These results emphasize the diversity of evolutionary processes shaping the genetic structure at different spatial scales. 相似文献
89.
R. L. Robbins 《Journal of fish biology》2007,70(5):1350-1364
The seasonal temporal sexual segregation of white sharks Carcharodon carcharias , at the Neptune Islands, South Australia, is described in relation to environmental variables. A significant sexual segregation was found at this site with sex ratios strongly favouring males overall. Males were more prevalent than females in all months in a 3 year sampling period, aside from April and May. There was a significant difference between the sexes in terms of mean daily numbers over the months of the study. It was also observed that sexual segregation fluctuated for unknown reasons on a temporal scale. Predictive models for estimating male and female numbers based on a series of abiotic factors were generated. Sea surface temperature, tidal height, tidal range, moon phase, cloud cover, underwater visibility and swell height were related to daily sightings of male and female white sharks to determine the influence of these variables on sexual segregation. Visibility and sea surface temperature affected male numbers, however, visibility did not affect sexual segregation. Tidal height was significantly different between males and females. Sea surface temperature also appeared to be related to sexual segregation in this species. Females were present when temperatures ranged from 15·7 to 18·1° C whereas males were observed at temperatures ranging from 14·3 to 17·8° C, with a peak in sightings in September, when sea surface temperatures were at their lowest. Since parturition is thought to occur in spring or summer, it is suggested that females are absent at this time and only return during prime feeding periods or times at which temperatures are elevated in order to increase developmental growth rates of their young. The significantly lower temperatures in 2003 may explain the absence of females in this year. Hypotheses related to temperature regulation in this species are put forward to explain the sexual segregation observed. 相似文献
90.
GÁBOR HERCZEG JARMO SAARIKIVI ABIGÉL GONDA JARMO PERÄLÄ AINO TUOMOLA JUHA MERILÄ 《Biological journal of the Linnean Society. Linnean Society of London》2007,92(1):19-27
In ectotherms, the main behavioural option for thermoregulation is the adjustment of daily and seasonal activity to the thermal quality of the environment. While active, ectotherms thermoregulate by shuttling in between thermally differing microhabitat patches. Here, we focused on the question of whether other behavioural or physiological processes could force ectotherms to maintain activity during thermally unfavourable periods, when accurate thermoregulation is impossible. Using laboratory experiments and field data we compared the thermoregulation of male adders ( Vipera berus ) between two periods in spring when (1) only males and (2) also females and juveniles had terminated their winter hibernation. We found that males thermoregulated actively both in the lab and in the field. Accurate thermoregulation was only possible during the second period because of the low thermal quality of the environment. Male adders maintained a lower mean body temperature in the field than in the laboratory within both periods, and in addition their body temperature during the first period was on average 4 °C lower than during the second period. The thermal qualities of the natural basking sites showed a similar pattern. We discuss the results in the context of a potential trade-off between spermiogenesis and thermoregulation, where the benefits of early spermiogenesis coupled with inaccurate thermoregulation are higher than the associated costs. The results support the contention that the earlier spring emergence of the male compared with female adders is explainable by natural selection favouring early initiation of spermiogenesis, and hence sex differences in phenology. © 2007 The Linnean Society of London, Biological Journal of the Linnean Society , 2007, 92 , 19–27. 相似文献