全文获取类型
收费全文 | 22512篇 |
免费 | 1777篇 |
国内免费 | 1332篇 |
出版年
2024年 | 52篇 |
2023年 | 434篇 |
2022年 | 423篇 |
2021年 | 974篇 |
2020年 | 1102篇 |
2019年 | 1477篇 |
2018年 | 962篇 |
2017年 | 614篇 |
2016年 | 724篇 |
2015年 | 790篇 |
2014年 | 1369篇 |
2013年 | 1603篇 |
2012年 | 1068篇 |
2011年 | 1390篇 |
2010年 | 1015篇 |
2009年 | 1067篇 |
2008年 | 1153篇 |
2007年 | 1176篇 |
2006年 | 1045篇 |
2005年 | 968篇 |
2004年 | 889篇 |
2003年 | 770篇 |
2002年 | 734篇 |
2001年 | 490篇 |
2000年 | 436篇 |
1999年 | 384篇 |
1998年 | 320篇 |
1997年 | 280篇 |
1996年 | 254篇 |
1995年 | 191篇 |
1994年 | 193篇 |
1993年 | 166篇 |
1992年 | 125篇 |
1991年 | 103篇 |
1990年 | 95篇 |
1989年 | 76篇 |
1988年 | 71篇 |
1987年 | 51篇 |
1986年 | 55篇 |
1985年 | 62篇 |
1984年 | 79篇 |
1983年 | 56篇 |
1982年 | 58篇 |
1981年 | 39篇 |
1980年 | 58篇 |
1979年 | 43篇 |
1978年 | 28篇 |
1977年 | 24篇 |
1976年 | 30篇 |
1974年 | 18篇 |
排序方式: 共有10000条查询结果,搜索用时 16 毫秒
11.
12.
One model for the timing of cytokinesis is based on findings that p34(cdc2) can phosphorylate myosin regulatory light chain (LC20) on inhibitory sites (serines 1 and 2) in vitro (Satterwhite, L.L., M.H. Lohka, K.L. Wilson, T.Y. Scherson, L.J. Cisek, J.L. Corden, and T.D. Pollard. 1992. J. Cell Biol. 118:595-605), and this inhibition is proposed to delay cytokinesis until p34(cdc2) activity falls at anaphase. We have characterized previously several kinase activities associated with the isolated cortical cytoskeleton of dividing sea urchin embryos (Walker, G.R., C.B. Shuster, and D.R. Burgess. 1997. J. Cell Sci. 110:1373-1386). Among these kinases and substrates is p34(cdc2) and LC20. In comparison with whole cell activity, cortical H1 kinase activity is delayed, with maximum levels in cortices prepared from late anaphase/telophase embryos. To determine whether cortical-associated p34(cdc2) influences cortical myosin II activity during cytokinesis, we labeled eggs in vivo with [(32)P]orthophosphate, prepared cortices, and mapped LC20 phosphorylation through the first cell division. We found no evidence of serine 1,2 phosphorylation at any time during mitosis on LC20 from cortically associated myosin. Instead, we observed a sharp rise in serine 19 phosphorylation during anaphase and telophase, consistent with an activating phosphorylation by myosin light chain kinase. However, serine 1,2 phosphorylation was detected on light chains from detergent-soluble myosin II. Furthermore, cells arrested in mitosis by microinjection of nondegradable cyclin B could be induced to form cleavage furrows if the spindle poles were physically placed in close proximity to the cortex. These results suggest that factors independent of myosin II inactivation, such as the delivery of the cleavage stimulus to the cortex, determine the timing of cytokinesis. 相似文献
13.
14.
15.
16.
Horim Lee 《Molecules and cells》2015,38(7):651-656
Plant growth and development are coordinately orchestrated by environmental cues and phytohormones. Light acts as a key environmental factor for fundamental plant growth and physiology through photosensory phytochromes and underlying molecular mechanisms. Although phytochromes are known to possess serine/threonine protein kinase activities, whether they trigger a signal transduction pathway via an intracellular protein kinase network remains unknown. In analyses of mitogen-activated protein kinase kinase (MAPKK, also called MKK) mutants, the mkk3 mutant has shown both a hypersensitive response in plant hormone gibberellin (GA) and a less sensitive response in red light signaling. Surprisingly, light-induced MAPK activation in wild-type (WT) seedlings and constitutive MAPK phosphorylation in dark-grown mkk3 mutant seedlings have also been found, respectively. Therefore, this study suggests that MKK3 acts in negative regulation in darkness and in light-induced MAPK activation during dark-light transition. 相似文献
17.
18.
19.
20.
Despite similar ecology, mating systems and female preferences for supernormal tails, the 17 species of African widowbirds and bishops (Euplectes spp.) show astonishing variation in male tail ornamentation. Whereas bishops retain their brown nonbreeding tails in nuptial plumage, widowbirds grow black nuptial tails, varying in length from a few centimetres in E. axillaris to the extreme half metre train of E. progne. Here, we phylogenetically reconstruct the evolution of the discrete trait, nuptial tail and the continuous trait, tail length, using a molecular phylogeny of 33 Euplectes subspecies. Unlike many recent findings of labile evolution of plumage ornaments, our results suggest that the nuptial tail of Euplectes is a derived and phylogenetically conserved ornamental trait that, once gained, shows directional evolution in its expression. Directionality is demonstrated in the trivial sense of a short‐tailed ancestor, and by contingency and randomization tests suggesting that branches with increasing tail length are overrepresented. This supports an early origin and strong retention of directional female mate choice in widowbirds and bishops, as previously indicated by empirical and experimental results, and provides a less labile, yet rapid scenario of sexually selected diversification. 相似文献