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71.
T. Olckers 《BioControl》1998,43(2):225-239
The South American tree Solanum mauritianum is a major environmental weed in the high-rainfall regions of South Africa and has been targeted for biological control. Potential agents included five species of the genus Platyphora, which were imported from South America in 1994. Platyphora species associated with Solanaceae reputedly have very specific habitat requirements and host plant preferences in the field. Despite this, host-specificity tests on one species, Platyphora semiviridis, revealed a broad physiological host range. Although laboratory tests showed that P. semiviridis is confined to Solanum species and cannot survive on solanaceous crops outside that genus, it developed on potato and cultivated eggplant (aubergine) as well as on 10 native South African Solanum species. With few exceptions, there were no consistent differences in survival and duration of development on these compared with S. mauritianum. Furthermore, at least six of these non-target species, including potato and eggplant, supported breeding colonies of the beetles in cages. During choice tests in both small and larger cages, P. semiviridis avoided potato but did not consistently discriminate between S. mauritianum, eggplant and six native solanums for larviposition. Despite these findings, P. semiviridis has never been recorded on either potato or eggplant in South America, where it was only observed to feed on S. mauritianum. Although there are several reasons why P. semiviridis is unlikely to attack non-target Solanum species in the field, it will not be released in South Africa because there are other imported agents which have displayed narrower physiological host ranges and which may be more effective. 相似文献
72.
Rolando De la Cruz Márcia D. Branco 《Biometrical journal. Biometrische Zeitschrift》2009,51(4):588-609
We have considered a Bayesian approach for the nonlinear regression model by replacing the normal distribution on the error term by some skewed distributions, which account for both skewness and heavy tails or skewness alone. The type of data considered in this paper concerns repeated measurements taken in time on a set of individuals. Such multiple observations on the same individual generally produce serially correlated outcomes. Thus, additionally, our model does allow for a correlation between observations made from the same individual. We have illustrated the procedure using a data set to study the growth curves of a clinic measurement of a group of pregnant women from an obstetrics clinic in Santiago, Chile. Parameter estimation and prediction were carried out using appropriate posterior simulation schemes based in Markov Chain Monte Carlo methods. Besides the deviance information criterion (DIC) and the conditional predictive ordinate (CPO), we suggest the use of proper scoring rules based on the posterior predictive distribution for comparing models. For our data set, all these criteria chose the skew‐t model as the best model for the errors. These DIC and CPO criteria are also validated, for the model proposed here, through a simulation study. As a conclusion of this study, the DIC criterion is not trustful for this kind of complex model. 相似文献
73.
Ting-Song Yang Xiao-Hu Yang Xi Chen Xu-Dong Wang Jie Hua Dong-Lei Zhou Bo Zhou Zhen-Shun Song 《FEBS letters》2014
It is well established that the interaction between cancer cells and microenvironment has a critical role in tumor development, but the roles of miRNAs in this interaction are rarely known. Here, we have shown that miR-106b is up-regulated in cancer associated fibroblasts compared with normal fibroblasts established from patients with gastric cancer, the expression level of miR-106b is associated with poor prognosis of patients, and CAFs with down-regulated miR-106b could significantly inhibit gastric cancer cell migration and invasion by targeting PTEN. Taken together, these data suggest that miR-106b might be a novel candidate target for the treatment of gastric cancer. 相似文献
74.
M. J. Travers I. C. Potter K. R. Clarke S. J. Newman J. B. Hutchins 《Journal of Biogeography》2010,37(1):148-169
Aim To test the following hypotheses regarding ichthyofaunal compositions along an extensive tropical coastline. The compositions over soft substrates and reefs: (1) consistently differ markedly; (2) change progressively with latitude and temperature through sequential changes in the abundances of certain species; and (3) vary among bioregions, as those regions differ markedly in their environmental characteristics. Location Tropical north‐western Australia. Methods Similarity matrices, derived from percentage contributions of each fish species to catches obtained over soft substrates by trawling and over reefs by trapping at seven regularly spaced sites along 1500 km of coast, were subjected to cluster analysis, non‐metric multidimensional scaling ordination and associated tests. Results In total, 361 species were obtained by trawling and trapping along the tropical coast of north‐western Australia (NWA). Only 56 species were recorded over both soft substrates and reefs, whereas 229 and 76 species were caught exclusively over soft substrates and reefs, respectively. The Leiognathidae, Carangidae and Terapontidae contributed most individuals (62.2%) to catches over soft substrates, whereas the Lethrinidae and Lutjanidae dominated those over reefs (81.9%). The species compositions in both habitats were related to latitude and water temperature. Ichthyofaunal compositions in the Kimberley region differed markedly from those in the Canning/Pilbara regions further south, which, in turn, each had distinct characters. The vast majority of species found over both habitats also occur in the Pacific Ocean to the north. Main conclusions The most important fish families over soft substrates and reefs in inshore marine waters of tropical NWA differ markedly. The ichthyofaunal compositions of both habitats undergo similar patterns of progressive change with latitude, due to site‐staggered changes in the relative abundances of key fish species in their respective habitats. Ichthyofaunal composition in both habitats was found to be influenced by water temperature. The latitudinal trends exhibited by species composition are overlaid on a strong bioregional effect, reflecting the influence of the very different environmental conditions in those bioregions, which include marked differences in such factors as tidal regime, turbidity and whether mangroves are nearby. The important contribution of species with a Pacific affinity was presumably facilitated by the polewards‐flowing Indonesian Throughflow, which links the Pacific Ocean with waters bordering the NWA coast. 相似文献
75.
Protein topology defined by the matrix of residue contacts has proved to be a fruitful basis for the study of protein dynamics. The widely implemented coarse-grained elastic network model of backbone fluctuations has been used to describe crystallographic temperature factors, allosteric couplings, and some aspects of the folding pathway. In the present study, we develop a model of protein dynamics based on the classical equations of motion of a damped network model (DNM) that describes the folding path from a completely unfolded state to the native conformation through a single-well potential derived purely from the native conformation. The kinetic energy gained through the collapse of the protein chain is dissipated through a friction term in the equations of motion that models the water bath. This approach is completely general and sufficiently fast that it can be applied to large proteins. Folding pathways for various proteins of different classes are described and shown to correlate with experimental observations and molecular dynamics and Monte Carlo simulations. Allosteric transitions between alternative protein structures are also modeled within the DNM through an asymmetric double-well potential. 相似文献
76.
77.
Sang Yun Kim Hyun Woo Lee Gha Young Lee Yun Sok Lee 《Biochemical and biophysical research communications》2010,392(3):323-24
microRNAs (miRNAs) are non-coding small RNAs regulating gene expression, cell growth, and differentiation. Although several miRNAs have been implicated in cell growth and differentiation, it is barely understood their roles in adipocyte differentiation. In the present study, we reveal that miR-27a is involved in adipocyte differentiation by binding to the PPARγ 3′-UTR whose sequence motifs are highly conserved in mammals. During adipogenesis, the expression level of miR-27a was inversely correlated with that of adipogenic marker genes such as PPARγ and adiponectin. In white adipose tissue, miR-27a was more abundantly expressed in stromal vascular cell fraction than in mature adipocyte fraction. Ectopic expression of miR-27a in 3T3-L1 pre-adipocytes repressed adipocyte differentiation by reducing PPARγ expression. Interestingly, the level of miR-27a in mature adipocyte fraction of obese mice was down-regulated than that of lean mice. Together, these results suggest that miR-27a would suppress adipocyte differentiation through targeting PPARγ and thereby down-regulation of miR-27a might be associated with adipose tissue dysregulation in obesity. 相似文献
78.
Simone Fattorini 《Journal of Biogeography》2007,34(6):994-1007
Aim To illustrate problems in the methods proposed by B. Vilenkin and V. Chikatunov to study levels of endemism and species–area relationships.
Location The study used data on the distribution of tenebrionid beetles (Coleoptera, Tenebrionidae) on the Aegean Islands (Greece).
Methods A total of 32 islands and 170 taxa (species and subspecies) were included in this study. Levels of endemism were evaluated both as the proportion of endemic taxa, and according to the methods proposed by Vilenkin and Chikatunov, which are based on the number of non-endemic taxa and various relationships with area. A model of the species–area relationship proposed by these authors was also analysed.
Results The number of endemic taxa was positively correlated with the number of taxa with different distribution types, but this positive correlation did not influence the estimation of the level of endemism. In fact, the commonly used estimate of endemicity as a percentage was strongly correlated with the endemism values calculated according to the method of Vilenkin and Chikatunov. The usual power function fitted the species–area relationship as well as the most complicated method of Vilenkin and Chikatunov.
Main conclusions As hypothesized by Vilenkin and Chikatunov, the number of endemic taxa was influenced both by the number of taxa of other biogeographical ranks, and by an island's area. However, explanations for the positive relationship between the number of endemic taxa and taxa of different biogeographical ranks are equivocal. Importantly, this relationship did not necessarily influence the level of endemism, which could be expressed adequately by percentages. The method proposed by Vilenkin and Chikatunov to estimate the species–area relationship cannot be clearly justified on theoretical grounds and is of questionable practical utility. 相似文献
Location The study used data on the distribution of tenebrionid beetles (Coleoptera, Tenebrionidae) on the Aegean Islands (Greece).
Methods A total of 32 islands and 170 taxa (species and subspecies) were included in this study. Levels of endemism were evaluated both as the proportion of endemic taxa, and according to the methods proposed by Vilenkin and Chikatunov, which are based on the number of non-endemic taxa and various relationships with area. A model of the species–area relationship proposed by these authors was also analysed.
Results The number of endemic taxa was positively correlated with the number of taxa with different distribution types, but this positive correlation did not influence the estimation of the level of endemism. In fact, the commonly used estimate of endemicity as a percentage was strongly correlated with the endemism values calculated according to the method of Vilenkin and Chikatunov. The usual power function fitted the species–area relationship as well as the most complicated method of Vilenkin and Chikatunov.
Main conclusions As hypothesized by Vilenkin and Chikatunov, the number of endemic taxa was influenced both by the number of taxa of other biogeographical ranks, and by an island's area. However, explanations for the positive relationship between the number of endemic taxa and taxa of different biogeographical ranks are equivocal. Importantly, this relationship did not necessarily influence the level of endemism, which could be expressed adequately by percentages. The method proposed by Vilenkin and Chikatunov to estimate the species–area relationship cannot be clearly justified on theoretical grounds and is of questionable practical utility. 相似文献
79.
Sun QiangLing Liu YinKun Lu WenJing Cheng Gang Zhou HaiJun Zhou XinWen Wei LiMing Dai Zhi Guo Kun Lu HaoJie 《中国科学C辑(英文版)》2007,50(3):403-411
In order to establish the novel high throughput, high efficiency and low cost technological platform for the research of N-glycoproteomics, to resolve the significance of characteristic expression profile of glycoprotein and to find the proteins
with biological functional importance, the glycoproteins with high-mannose core and the two antennary types were purified
and enriched by the Con A affinity chromatography. Con A affinity protein expression profiles of normal human liver tissue
were generated by using SDS-PAGE, two-dimensional electrophoresis (2-DE) followed by fast fluorescence staining based on multiplexed
proteomics (MP) technology. 301 visible protein spots on the gel were detected and 85 of glycoproteins were further successfully
identified via peptide mass fingerprinting (PMF) by a matrix assisted laser desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF-MS/MS) and annotated to IPI databases. Identified glycoproteins definitely take part in the regulation of cell
cycle and metabolic processes. The glycosylation sites were predicted with NetNGlyc 1.0 and NetOGlyc 3.1 software, meanwhile
they were classified according to the geneontology methods. The construction of Con A affinity glycoprotein database of normal
human liver tissue would contribute to the subsequent research. 相似文献
80.
Bryanne M. Hoar Douglas P. Whiteside Linda Ward G. Douglas Inglis Douglas W. Morck 《Zoo biology》2007,26(2):141-153
The enteric flora of captive whooping cranes (Grus americana) and sandhill cranes (Grus canadensis) has not been well described, despite its potential importance in the understanding of both the normal condition of the intestinal physiology of these animals and the altered colonization within disease states in these birds. Nineteen whooping cranes and 23 sandhill cranes housed currently at the Calgary Zoo or its affiliated Devonian Wildlife Conservation Centre (DWCC) in Calgary, Alberta were sampled from October 2004–February 2005 by collecting aerobic and anaerobic cloacal swabs from each bird. There were seven major groupings of bacteria isolated from both species of crane. Gram‐positive cocci, coliforms, and gram‐negative bacilli were the most prevalent types of bacteria isolated for both crane species, with Escherichia coli, Enterococcus faecalis, and Streptococcus Group D, not Enterococcus the bacterial species isolated most commonly. There was a significant difference in the average number of isolates per individual between the two crane species but no differences between age or gender categories within crane species. Campylobacter sp. were isolated from five whooping cranes. The potential zoonotic pathogen Campylobacter jejuni was isolated from one whooping crane and C. upsaliensis was isolated from a second. Three other isolates were unspeciated members of the Campylobacter genus and likely belong to a species undescribed previously. The evaluation of the enteric cloacal flora of whooping cranes and sandhill cranes illustrates that differences exist between these two closely related crane species, and highlights the potential implications these differences may have for current practices involving captive wildlife. Zoo Biol 0:1–13, 2007. © 2007 Wiley‐Liss, Inc. 相似文献