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981.
Kamonrat Phopin Wutigri Nimlamool Linda J. Lowe‐Krentz Elijah W. Douglass Jaclyn N. Taroni Barry S. Bean 《Molecular reproduction and development》2013,80(4):273-285
Sperm‐associated α‐L ‐fucosidases have been implicated in fertilization in many species. Previously, we documented the existence of α‐L ‐fucosidase in mouse cauda epididymal contents, and showed that sperm‐associated α‐L ‐fucosidase is cryptically stored within the acrosome and reappears within the sperm equatorial segment after the acrosome reaction. The enrichment of sperm membrane‐associated α‐L ‐fucosidase within the equatorial segment of acrosome‐reacted cells implicates its roles during fertilization. Here, we document the absence of α‐L ‐fucosidase in mouse oocytes and early embryos, and define roles of sperm associated α‐L ‐fucosidase in fertilization using specific inhibitors and competitors. Mouse sperm were pretreated with deoxyfuconojirimycin (DFJ, an inhibitor of α‐L ‐fucosidase) or with anti‐fucosidase antibody; alternatively, mouse oocytes were pretreated with purified human liver α‐L ‐fucosidase. Five‐millimolar DFJ did not inhibit sperm–zona pellucida (ZP) binding, membrane binding, or fusion and penetration, but anti‐fucosidase antibody and purified human liver α‐L ‐fucosidase significantly decreased the frequency of these events. To evaluate sperm‐associated α‐L ‐fucosidase enzyme activity in post‐fusion events, DFJ‐pretreated sperm were microinjected into oocytes, and 2‐pronuclear (2‐PN) embryos were treated with 5 mM DFJ with no significant effects, suggesting that α‐L ‐fucosidase enzyme activity does not play a role in post‐fusion events and/or early embryo development in mice. The recognition and binding of mouse sperm to the ZP and oolemma involves the glycoprotein structure of α‐L ‐fucosidase, but not its catalytic action. These observations suggest that deficits in fucosidase protein and/or the presence of anti‐fucosidase antibody may be responsible for some types of infertility. Mol. Reprod. Dev. 80: 273–285, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
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Julian E. Andrews 《Ichnos》2013,20(4):247-253
The Kilmaluag Formation of the Great Estuarine Group (Middle Jurassic) of Scotland represents deposition of mixed carbonate and clastic sediments in a low‐salinity coastal lagoon to floodplain lake setting. Large, unusual trace fossils occur at two horizons within the formation. One type consists of platelike structures about 50 cm in diameter, which are found on wave‐rippled sandstone. These structures, strikingly similar to burrows produced by modern mudskippers, are assigned to fish that shallowly burrowed into the lagoon‐shore sediment. The second type of burrows, found in brecciated, dolomitic limestones, are pipelike, about 4 to 7 cm in diameter and as much as 50 cm deep. One example has a chamber at the base of the pipe. Although most features of these structures appear similar to modern lungfish burrows, the chamber is most similar to structures produced by modern crayfish. The animal probably burrowed into the moist, mudflat sediment to escape desiccation during seasonal aridity. 相似文献
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