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951.
Nuclear magnetic resonance studies of the location and function of plant nutrients in vivo 总被引:2,自引:1,他引:1
The cytoplasmic and vacuolar pools of ammonium, inorganic phosphate and potassium can be studied non-invasively in plant tissues using high resolution nuclear magnetic resonance spectroscopy. The techniques that allow these pools to be discriminated in vivo are described and their application to plants is reviewed with reference to the phosphorus, nitrogen and potassium nutrition of root tissues. 相似文献
952.
Effects of aluminium on growth and cation uptake in seedlings of Eucalyptus mannifera and Pinus radiata 总被引:1,自引:0,他引:1
This experiment was designed to examine the effects of aluminium (Al) on the growth of Pinus radiata (D. Don) and Eucalyptus mannifera subsp. mannifera (Mudie) seedlings in culture solutions in a glasshouse to help explain the failure of radiata pine trees on some acid, low
fertility soils in Australia on which the native eucalypts flourish. Aluminium (Al) in culture solution increased the growth
of roots and shoots of seedlings of both species but while growth of the eucalypt continued to increase with increases in
Al to 2.222 μM, growth of the pine was largest at 370 μM Al. In addition to total root length, specific root length (length per unit dry weight), a measure of fineness of the root,
increased in the eucalypt seedlings as the substrate Al increased. Growth of the shoots and roots of the pine in the absence
of any added Al was extremely poor suggesting that Al, in low concentrations, may be an essential element or ameliorate some
other factors in solution culture at low pH.
Root and shoot concentrations of K increased with increasing Al, whilst Ca and Mg Concentrations decreased and Mn concentrations
were unaffected in both species. Tissue Ca and Mg concentrations were 2 to 3 times higher in the eucalypt seedlings than the
pine at all levels of added Al due to greater uptake of these elements by the eucalypt. In contrast, at the highest concentration
of Al in the medium, shoot Al concentrations were lower in the cucalypt than in the pine due to a greater proportion of Al
being retained in the eucalypt roots.
These differences between the seedlings in terms of root growth and tissue cation concentrations may help explain the ability
of eucalypt species to maintain vigorous growth on acid soils high in Al and low in Ca and P, where growth of the pines failed. 相似文献
953.
A relatively drought tolerant cultivar of maize ( Zea mays L. cv. Pioneer 3950) and a drought tolerant line of sorghum ( Sorghum bicolor [L.] Moench cv. ICSV 112) were grown hydroponically for 11 days. Treatments for non-ionic osmotic and salt stresses were started at the 8th day by addition of polyethylene glycol 6000 and NaCl, respectively, at 200 mOsm equivalent concentrations in the presence or absence of 0. 1 μ M abscisic acid. Relative growth rate was depressed by both stress factors, more severely for maize than sorghum. Abscisic acid increased the growth rate and reverted the negative effect of NaCl in maize, while sorghum was only slightly affected. In general, sorghum had higher levels of K+ and lower levels of Na+ and the K+ /Na+ ratio was further increased by abscisic acid treatment. From the pressure-volume curves, osmotic potential, the water potential at turgor loss point, bulk elastic modulus and the water saturation deficit at initial turgor loss were estimated. Most significantly, sorghum had a higher elastic modulus than maize and it decreased under osmotic treatment, while in maize it increased under NaCl stress. The results suggest that bulk tissue turgor was not limiting growth under these conditions and underscores the possible implications of changes in the elastic condition of the cell walls in stress responses. 相似文献
954.
Effect of nitrogen supply on frost resistance, nitrogen metabolism and carbohydrate content in white clover (Trifolium repens) 总被引:1,自引:0,他引:1
Nina Sandli Mette M. Svenning Kirsti Røsnes Olavi Junttila 《Physiologia plantarum》1993,88(4):661-667
Effects of mineral nitrogen (2, 4, 6 and 8 m M NH4 NO3 ) and nodulation with Rhizobium on frost hardiness in seedlings of white clover ( Trifolium repens ) have been studied. Seedlings of a population from Bodø (67°N lat.) were grown in Leonard jars under controlled conditions in a phytotron. For induction of frost hardening, plants were first exposed to 12 h photoperiod conditions for 2 weeks at 18°C, then for 2 weeks at 6°C and finally for 2 weeks at 0.5°C. Frost hardiness after treatments at 6 and 0.5°C was significantly enhanced by increasing nitrogen supply and was positively correlated with total nitrogen content of the stolons. Frost hardiness of nodulated plants correlated to the tissue nitrogen concentration. Content of soluble proteins in stolons decreased during hardening at 6°C but did not change during treatment at 0.5°C. There were minor changes in total amount of free amino acids during hardening. Both absolute and relative amounts of proline and arginine increased, and those of asparagine decreased during hardening. Absolute amounts of all free amino acids increased with increasing nitrogen supply, but the changes during hardening were similar in all treatments. There was a significant increase in the content of soluble carbohydrates during hardening. However, this increase was inversely related to nitrogen supply. 相似文献
955.
Annexin-mediated secretory vesicle aggregation in plants 总被引:4,自引:0,他引:4
The mechanism by which membranes fuse during vesicle-mediated secretion is of considerable importance for plant cell growth, but remains unknown. We have identified Ca2+ -dependent phospholipid-binding proteins (annexins) from maize ( Zea mays ), that may play a part in this process. An assay for Ca2+ -dependent binding of annexins to liposomes, revealed that the maize proteins (p23, p33 and p35) and annexins from bovine lung, bind over a similar range of Ca2+ concentrations. Turbidity assays further revealed that both maize and bovine annexins induced liposome aggregation and that the plant annexins were also effective at aggregating plant secretory vesicles. This aggregation occurred at levels of free Ca2+ similar to that required for the binding of annexins p33 and p35. We discuss the significance of these results for the plant secretory apparatus. 相似文献
956.
Estimates of the residual nitrogen benefit of groundnut to maize in Northeast Thailand 总被引:4,自引:1,他引:3
Four cultivars of groundnut were grown in upland soil in Northeast Thailand to study the residual benefit of the stover to a subsequent maize crop. An N-balance estimate of the total residual N in the maize supplied by the groundnut was made. In addition three independent estimates were made of the residual benefits to maize when the groundnut stover was returned to the land and incorporated. The first estimate (Estimate 1) was an N-balance estimate. A dual labelling approach was used where 15N-labelled stover was added to unlabelled microplots (Estimate 2) or unlabelled stover was added to 15N-labelled soil microplots (Estimate 3). The nodulating groundnut cultivars fixed between 59–64% of their nitrogen (as estimated by the 15N isotope dilution method using non-nodulating groundnut as a non-fixing reference) producing between 100 and 130 kg N ha-1 in their stover. Although the following maize crop suffered from drought stress, maize grain N and dry weights were up to 80% and 65% greater respectively in the plots where the stover was returned as compared with the plots where the stover was removed. These benefits were comparable with applications of 75 kg N ha-1 nitrogen in the form of urea. The total residual N estimates of the contribution of the nodulated groundnut to the maize ranged from 16.4–27.5 kg N ha-1. Estimates of the residual N supplied by the stover and fallen leaves ranged from 11.9–21.3 kg N ha-1 using the N-balance method (Estimate 1), from 6.3–9.6 kg N ha-1 with the labelled stover method (Estimate 2) and from 0–11.4 kg N ha-1 with the labelled soil method. There was closest agreement between the two 15N based estimates suggesting that apparent added nitrogen interactions in these soils may not be important and that N balance estimates can overestimate the residual N in crops following legumes, even in very poor soils. This work also indicates the considerable ability of local groundnut cultivars to fix atmospheric nitrogen and the potential benefits from returning and incorporating legume residues to the soil in the upland cropping systems of Northeast Thailand. The applicability of the 15N methodology used here and possible reasons for the discrepancies between estimates 1, 2 and 3 are discussed. 相似文献
957.
Inhibition of nitrate uptake by aluminium in maize 总被引:1,自引:0,他引:1
Experiments with two maize (Zea mays L.) hybrids were conducted to determine (a) if the inhibition of nitrate uptake by aluminium involved a restriction in the
induction (synthesis/assemblage) of nitrate transporters, and (b) if the magnitude of the inhibition was affected by the concurrent
presence of ambient ammonium. At pH 4.5, the rate of nitrate uptake from 240 μM NH4NO3 was maximally inhibited by 100 μM aluminium, but there was little measurable effect on the rate of ammonium uptake. Presence of ambient aluminium did not eliminate
the characteristic induction pattern of nitrate uptake upon first exposure of nitrogen-depleted seedlings to that ion. Removal
of ambient aluminium after six hours of induction resulted in recovery within 30 minutes to rates of nitrate uptake that were
similar to those of plants induced in absence of aluminium. Addition of aluminium to plants that had been induced in absence
of aluminium rapidly restricted the rate of nitrate uptake to the level of plants that had been induced in the presence of
aluminium. The data are interpreted as indicating that aluminium inhibited the activity of nitrate transporters to a greater
extent than the induction of those transporters. When aluminium was added at initiation of induction, the effect of ambient
ammonium on development of the inhibition by aluminium differed between the two hybrids. The responses indicate a complex
interaction between the aluminium and ammonium components of high acidity soils in their influence on nitrate uptake. ei]{gnA
C}{fnBorstlap} 相似文献
958.
Defining phosphorus efficiency in plants 总被引:6,自引:0,他引:6
The many different definitions for "nutrient efficiency" make the use of the term ambiguous. We evaluated nutrient efficiency using data from a study of response to phosphorus (P) supply in white clover (Trifolium repens L.) and lucerne (Medicago sativa L.). Application of various criteria identified in the literature as measures of nutrient efficiency did not clarify differences between purportedly P efficient and inefficient germplasm. Germplasm differed in maximum shoot and total dry mass and in solution P concentration ([P]s) required to achieve 80% maximum yield, but not in P concentration of tissue ([P]t), internal P utilization, or P uptake per unit of fine root dry mass. Differences in yield may have resulted from factors other than efficient use of P. To reduce the confounding effects that other factors have on nutrient efficiency, it is essential that equivalent yields of germplasm be demonstrated where nutrients are not limiting. Mechanisms that enable enhanced nutrient efficiency can be identified less ambiguously using this approach.Joint contribution of the Minn. Agric. Exp. Stn. and the USDA-ARS 相似文献
959.
At two sites, one with a 4-year-old (4-Y) secondary vegetation and the other with a 20-year-old (20-Y) vegetation, the influence of burning slashed vegetation on crop performance was studied during three seasons. In the first season after clearing, also the influence on weed growth was studied. At both sites, burning significantly decreased the number of weed seedlings. The lowest number of seedlings was found on the burnt plots of the 20-Y site. Burning increased yield and nutrient uptake significantly in the first and second season after clearing. In the third season after burning, only at the 4-Y site a significantly higher yield and nutrient uptake were found. At the 20-Y site the effect had disappeared. Calculations of efficiency of utilization of absorbed N, P and K indicated that P was the least available nutrient, also after burning. At both sites three consecutive crops absorbed approximately 40% of P applied in ash, while the cumulative recovery of K was at least 36% at the 4-Y site and at least 59% at the 20-Y site. On non-burnt plots, yields were not lower in the third season than in the first season after clearing, thus indicating that the inherent soil fertility did not decrease. Hence, yield decline on the burnt plots could be ascribed to ash depletion. It was concluded that in the local shifting cultivation system, the combination of ash depletion and infestation of weeds are the main reasons for abandoning the fields. 相似文献
960.
Nitrogen losses in puddled soils as affected by timing of water deficit and nitrogen fertilization 总被引:2,自引:0,他引:2
Erratic rainfall in rainfed lowlands and inadequate water supply in irrigated lowlands can results in alternate soil drying and flooding during a rice (Oryza sativa L.) cropping period. Effects of alternate soil drying and flooding on N loss by nitrification-denitrification have been inconsistent in previous field research. To determine the effects of water deficit and urea timing on soil NO3 and NH4, floodwater NO3, and N loss from added 15N-labeled urea, a field experiment was conducted for 2 yr on an Andaqueptic Haplaquoll in the Philippines. Water regimes were continuously flooded, not irrigated from 15 to 35 d after transplanting (DT), or not irrigated from 41 to 63 DT. The nitrogen treatments in factorial combination with water regimes were no applied N and 80 kg urea-N ha–1, either applied half basally and half at 37 DT or half at 11 DT and half at 65 DT. Water deficit at 15 to 35 DT and 41 to 63 DT, compared with continuous soil flooding, significantly reduced extractable NH4 in the top 30-cm soil layer and resulted in significant but small (<1.0 kg N ha–1) soil NO3 accumulations. Soil NO3, which accumulated during the water deficit, rapidly disappeared after reflooding. Water deficit at 15 to 35 DT, unlike that at 41 to 63 DT, increased the gaseous loss of added urea N as determined from unrecovered 15N in 15N balances. The results indicate that application of urea to young rice in saturated or flooded soil results in large, rapid losses of N (mean = 35% of applied N), presumably by NH3 volatilization. Subsequent soil drying and flooding during the vegetative growth phase can result in additional N loss (mean = 14% of applied N), presumably by nitrification-denitrification. This additional N loss due to soil drying and flooding decreases with increasing crop age, apparently because of increased competition by rice with soil microorganisms for NH4 and NO3. 相似文献