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131.
Sappinia sp. (Amoebozoa: Thecamoebida) and Rosculus sp. (SAR: Cercozoa) Isolated From King Penguin Guano Collected in the Subantarctic (South Georgia,Salisbury Plain) and their Coexistence in Culture 下载免费PDF全文
Two amoeboid organisms of the genera Sappinia Dangeard, 1896 and Rosculus Hawes, 1963 were identified in a sample containing king penguin guano. This sample, collected in the Subantarctic, enlarges the list of fecal habitats known for the presence of coprophilic amoebae. The two organisms were co‐isolated and subcultured for over 6 mo, with continuous efforts being invested to separate each one from the mixed culture. In the mixed culture, Rosculus cells were fast growing, tolerated changes in culturing conditions, formed cysts, and evidently were attracted by Sappinia trophozoites. The separation of the Rosculus strain was accomplished, whereas the Sappinia strain remained intermixed with inseparable Rosculus cells. Sappinia cell populations were sensitive to changes in culturing conditions; they improved with reduction of Rosculus cells in the mixed culture. Thick‐walled cysts, reportedly formed by Sappinia species, were not seen. The ultrastructure of both organisms was congruent with the currently accepted generic characteristics; however, some details were remarkable at the species level. Combined with the results of phylogenetic analyses, our findings indicate that the ultrastructure of the glycocalyx and the presence/absence of the Golgi apparatus in differential diagnoses of Sappinia species require a critical re‐evaluation. 相似文献
132.
转移生长因子β(TGFβ)信号传导通路参与调节细胞的增殖、分化、凋亡、细胞迁移等一系列细胞过程,与骨代谢疾病的发病机制密切相关.本研究根据荧光共振能量转移(FRET)技术原理,构建包含CFP-TβRI-YFP融合蛋白的TβRI生物传感器,转染293T细胞,观察转染效率.以TGFβ1为诱导剂,激活TGFβ/TβRI信号传导通路,在活细胞生理条件下,动态监测TβRI生物传感器的FRET效应.结果表明,成功构建了TβRI生物传感器,转染细胞效率达50%,在TGFβ1诱导刺激6min后,FRET效率增加并达到最大值,该过程经历9 min后,随时间的延长,FRET效率下降.研究结果表明:在活细胞生理条件下,TGFβ1/TβRI信号转导过程存在一定的时间特异性. 相似文献
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134.
《Bioscience, biotechnology, and biochemistry》2013,77(2):454-457
A new method called “Aqua-space®” was developed for the isolation of the natural fragrances of plants. Living flowers were enclosed in a space under simulated natural conditions, and humidified air was pumped into the space as a fragrance carrier. In a comparison among three isolation methods, i.e., Aqua-space®, headspace, and solvent extraction, the Aqua-space® method proved to be the most effective in retaining natural fragrances with abundant oxygenated components key to floral fragrances. 相似文献
135.
Design of a phosphinate‐based bioluminescent probe for superoxide radical anion imaging in living cells 下载免费PDF全文
Superoxide radical anion (O2˙?) as an important member of reactive oxygen species (ROS) plays a vital role both in physiology and pathology. Herein we designed and synthesized a novel phosphinate‐based bioluminescence probe for O2˙? detection in living cells, which exhibited good sensitivity for capturing O2˙? at the nanomole level and high selectivity against other ROS. The probe was further found to be of low toxicity for living cells and was then successfully employed for sensing endogenous O2˙? by using phorbol‐12‐myristate‐13‐acetate (PMA) as a traditional O2˙? stimulator in Huh7 cells. Moreover, the increasing production and use of nanoparticles, has given rise to many concerns and debates among the public and scientific authorities regarding their safety and final fate in biological systems. Herein it was found that mondisperse polystyrene particles could stimulate O2˙? generation in Huh7 cells. Overall, the probe was demonstrated to have a great potential as a novel bioluminescent sensor for detecting O2˙? in living cells. To our knowledge, this is the first small‐molecule phosphinate‐based bioluminescence probe that will open up great opportunities for unlocking the mystery of O2˙? in human health and disease. 相似文献
136.
The importance of substrate type, shading and scorching for the attractiveness of dead wood to saproxylic beetles 总被引:1,自引:0,他引:1
J. Hjltn T. Johansson O. Alinvi K. Danell J.P. Ball R. Pettersson H. Gibb J. Hilszczaski 《Basic and Applied Ecology》2007,8(4):364-376
Modern forestry management has reduced the amount of dead wood in forest ecosystems and this has become a serious threat to flora and fauna. Efforts are therefore being made to reverse this trend but one problem is that we still lack detailed knowledge regarding the substrate requirements of many saproxylic species. In a field experiment, conducted in three forest types (forest reserve, mature managed forest and clear-cut), we evaluated the value, from a conservation perspective, of different substrate types (logs, snags and tops) of Norway Spruce, Picea abies, and if the quality of spruce logs as saproxylic habitats can be improved by simple log treatments (scorching and shading). We collected 9982 individuals representing 262 saproxylic beetle species in window traps. Both substrate type and, to a lesser extent, log treatment had a significant effect on the abundance and species richness of saproxylic beetles attracted to the different dead wood substrates. However, more importantly, the composition of the beetle assemblages differed significantly between both substrates and log treatments. Snags, logs and tops all attracted significantly different beetle assemblages and scorched logs differed from untreated control logs. Sixteen red listed species were trapped, with the highest number (11 species) being found on scorched logs. We found strong evidence that some species preferred a specific substrate type, mainly logs, in some cases treated logs (scorched or shaded), but not snags, the substrate commonly provided for conservation purposes on e.g. clear-cuts. This stresses the importance of conducting forestry in such a way that a multitude of both forest habitats and dead wood substrates are available continuously in the forest landscape to maintain biodiversity. 相似文献
137.
绿色屋顶是雨洪管理中一项重要的低影响开发(LID)措施。由于气候变化和城市快速发展,LID雨洪管理策略对于当下全球的城市设计都至关重要,尤其是在经济蓬勃发展历来又饱受洪水灾害的中国。气候变化导致降水事件及雨水径流峰值流速难以准确预测。面对建筑布局日益稠密、不透水下垫面日益增多的中国城市,设计师们需要随时做好应对城市雨洪问题的准备。2015年提出的“海绵城市”战略为在城市尺度下应用和监测绿色屋顶效益提供了机会。目前业内大多数国际性绿色屋顶的研究应用都局限于小型控制实验区,借海绵城市建设之东风有机会将应用实验扩展到城市规模。中国雨洪管理智慧的历史可以追溯至5 000年前,现今中国正处于成为大尺度绿色屋顶实践世界领先者的机遇期。因此,LID应是城市设计的驱动力量而非事后补救措施。景观设计师不仅能够从场地、城市和区域3个层面上对场地及其周围环境进行全面解读,而且对需要实施LID的其他因素也有全面认知。为探究中国雨洪管理结构提供了新的视角,对景观设计师如何参与LID实践提出了建议。 相似文献
138.
2010秋季东海今生颗石藻的空间分布 总被引:1,自引:0,他引:1
根据2010年11月东海29站位所采集108个样品偏光镜检分析结果显示:调查区共发现26种今生颗石藻,优势种为赫氏艾密里藻(Emiliania huxleyi)、大洋桥石藻(Gephyrocapsa oceanica)、卡特螺旋球藻(Helicosphaera carteri)和粗壮环翼球(Algirosphaera robusta)等.水体中今生颗石藻丰度为0-76.562个/mL,平均值为18.641个/mL;颗石粒丰度0-4506.47个/mL,平均值为613.44个/mL,今生颗石藻在表层和底层均呈斑块状分布,海盆区站位丰度较近岸站位下降明显,但物种丰富度明显增多;颗石球丰度最大值站位发生在硅藻水华站位.将研究区域划分为3个断面进行分析:PN断面、沿岸流断面和黑潮断面.比较显示,PN断面的空间分布差异性较大,赫氏艾密里藻具有最高丰度的颗石粒和颗石球.沿岸流断面颗石粒/颗石球、黑潮断面中颗石粒(除底层外),自表至底随水深增加有轻微增加趋势,黑潮断面颗石球丰度高值区集中于25-80 m之间.结合历史环境资料,分析得出东海调查期水体混合、泥沙再悬浮以及硝酸盐浓度是控制今生颗石藻物种丰富度与物种特异分布的主要因子;今生颗石藻在不同环境中具有不同的生活策略,其空间分布特征随取样季节和海区不同有较大变化. 相似文献
139.
140.
Michael G. Gardner Sarah K. Pearson Gregory R. Johnston Michael P. Schwarz 《Biological reviews of the Cambridge Philosophical Society》2016,91(4):925-936
How sociality evolves and is maintained remains a key question in evolutionary biology. Most studies to date have focused on insects, birds, and mammals but data from a wider range of taxonomic groups are essential to identify general patterns and processes. The extent of social behaviour among squamate reptiles is under‐appreciated, yet they are a promising group for further studies. Living in aggregations is posited as an important step in the evolution of more complex sociality. We review data on aggregations among squamates and find evidence for some form of aggregations in 94 species across 22 families. Of these, 18 species across 7 families exhibited ‘stable’ aggregations that entail overlapping home ranges and stable membership in long‐term (years) or seasonal aggregations. Phylogenetic analysis suggests that stable aggregations have evolved multiple times in squamates. We: (i) identify significant gaps in our understanding; (ii) outline key traits which should be the focus of future research; and (iii) outline the potential for utilising reproductive skew theory to provide insights into squamate sociality. 相似文献