A spatial genetics approach to inform vector control of tsetse flies (Glossina fuscipes fuscipes) in Northern Uganda

Tsetse flies (genus Glossina) are the only vector for the parasitic trypanosomes responsible for sleeping sickness and nagana across sub‐Saharan Africa. In Uganda, the tsetse fly Glossina fuscipes fuscipes is responsible for transmission of the parasite in 90% of sleeping sickness cases, and co‐occurrence of both forms of human‐infective trypanosomes makes vector control a priority. We use population genetic data from 38 samples from northern Uganda in a novel methodological pipeline that integrates genetic data, remotely sensed environmental data, and hundreds of field‐survey observations. This methodological pipeline identifies isolated habitat by first identifying environmental parameters correlated with genetic differentiation, second, predicting spatial connectivity using field‐survey observations and the most predictive environmental parameter(s), and third, overlaying the connectivity surface onto a habitat suitability map. Results from this pipeline indicated that net photosynthesis was the strongest predictor of genetic differentiation in G. f. fuscipes in northern Uganda. The resulting connectivity surface identified a large area of well‐connected habitat in northwestern Uganda, and twenty‐four isolated patches on the northeastern margin of the G. f. fuscipes distribution. We tested this novel methodological pipeline by completing an ad hoc sample and genetic screen of G. f. fuscipes samples from a model‐predicted isolated patch, and evaluated whether the ad hoc sample was in fact as genetically isolated as predicted. Results indicated that genetic isolation of the ad hoc sample was as genetically isolated as predicted, with differentiation well above estimates made in samples from within well‐connected habitat separated by similar geographic distances. This work has important practical implications for the control of tsetse and other disease vectors, because it provides a way to identify isolated populations where it will be safer and easier to implement vector control and that should be prioritized as study sites during the development and improvement of vector control methods.     

Large‐scale distribution patterns of mangrove nematodes: A global meta‐analysis

Mangroves harbor diverse invertebrate communities, suggesting that macroecological distribution patterns of habitat‐forming foundation species drive the associated faunal distribution. Whether these are driven by mangrove biogeography is still ambiguous. For small‐bodied taxa, local factors and landscape metrics might be as important as macroecology. We performed a meta‐analysis to address the following questions: (1) can richness of mangrove trees explain macroecological patterns of nematode richness? and (2) do local landscape attributes have equal or higher importance than biogeography in structuring nematode richness? Mangrove areas of Caribbean‐Southwest Atlantic, Western Indian, Central Indo‐Pacific, and Southwest Pacific biogeographic regions. We used random‐effects meta‐analyses based on natural logarithm of the response ratio (lnRR) to assess the importance of macroecology (i.e., biogeographic regions, latitude, longitude), local factors (i.e., aboveground mangrove biomass and tree richness), and landscape metrics (forest area and shape) in structuring nematode richness from 34 mangroves sites around the world. Latitude, mangrove forest area, and forest shape index explained 19% of the heterogeneity across studies. Richness was higher at low latitudes, closer to the equator. At local scales, richness increased slightly with landscape complexity and decreased with forest shape index. Our results contrast with biogeographic diversity patterns of mangrove‐associated taxa. Global‐scale nematode diversity may have evolved independently of mangrove tree richness, and diversity of small‐bodied metazoans is probably more closely driven by latitude and associated climates, rather than local, landscape, or global biogeographic patterns.     

Separating the effects of water quality and urbanization on temperate insectivorous bats at the landscape scale

Many local scale studies have shown that bats respond to water quality degradation or urbanization in a species‐specific manner. However, few have separated the effects of urbanization versus water quality degradation on bats, in single city or single watershed case studies. Across North Carolina, USA, we used the standardized North American Bat Monitoring Program mobile transect protocol to survey bat activity in 2015 and 2016 at 41 sites. We collected statewide water quality and urban land cover data to disentangle the effects of urbanization and water quality degradation on bats at the landscape scale. We found that statewide, water quality degradation and urbanization were not correlated. We found that bats responded to water quality degradation and urbanization independently at the landscape scale. Eptesicus fuscus and Lasiurus cinereus negatively responded to water quality degradation. Lasiurus borealis and Perimyotis subflavus positively responded to water quality degradation. Lasionycteris noctivagans did not respond to water quality degradation but was more active in more urbanized areas. Tadarida brasiliensis positively responded to urbanization and was less active in areas with degraded water quality. We show that bat–water quality relationships found at the local scale are evident at a landscape scale. We confirm that bats are useful bioindicators for both urbanization and water quality degradation. We suggest that water quality can be used to predict the presence of bat species of conservation concern, such as P. subflavus, in areas where it has not been studied locally.     

Antibody-antigen recognition: A canonical structure paradigm

The antibodies of known three-dimensional structure exhibit a definite number of conformations (canonical structures) for five of six hypervariable loops. In the present study it was found that approximately 85% of the immunoglobulin sequences analyzed fall into a small number of canonical structure combinations, representing only 3% of the total possible. These structures were classified into six distinct groups, depending on the type of antigen with which they interact.Within each loop, the positions responsible for maintaining these canonical structures show a use frequency of amino acids that fits an inverse power law, whereas the use frequency of the amino acids responsible for the detailed antigenic specificity follows an exponential distribution. We propose an evolutionary interpretation that connects these data, using the fact that the inverse power law is generated by statistical processes of the type that yield a wealth curve and the fact that expoential distribution is generated by processes that are not biased by past history.     

景观多样性分析及其制图研究

景观多样性是生物多样性研究的主要内容之一。景观多样性是指景观结构、功能和动态的多样性和复杂性。本文讨论了景观多样性的概念、测度和空间制图。提出了景观多样性包括类型多样性、格局多样性和斑块多样性。景观多样性可用多样性指数、优势度、相对丰富度、修改的分维数等指数来测定。一个多样性指数Ｄ＿ｉ＝（ｉ－１）／（ｍ－１）×１００％被给出用于景观多样性制图,黄土高原泉家沟流域被选为研究区域。在地理信息系统的支持下,通过时栅格化的土地利用类型图进行分析操作产生出了研究区域的景观多样性图。对景观多样性进行空间制图有助于分析景观生态过程,研究结果可直接用于景观和土地利用规划、野生动植物保护及立地分析。     

试论景观的动态变化

试论景观的动态变化孙中伟（中国科学院沈阳应用生态研究所１１００１５）ＯｎＲｅｓｅａｒｃｈｅｓｏｆＬａｎｄｓｃａｐｅＤｙｎａｍｉｃＣｋａｎｇｅ．￥ＳｕｎＺｈｏｎｇｗｅｉ（ＩｎｓｔｉｔｕｔｅｏｆＡｐｐｌｉｅｄＥｃｏｌｏｇｙ,ＡｃａｄｅｍｉａＳｉｎｉｃａ,...     

探索景观异质性的热力学基础和信息论

探索景观异质性的热力学基础和信息论ＭｉｃｈｅｌＧｏｄｒｏｎ,ＪａｃｑｕｅｓＢａｕｄｒｙＲｉｃｈａｒｄＴ．Ｔ．Ｆｏｒｍａｎ）ＴｈｅｒｍｏｄｙｎａｍｉｃＦｏｕｎｄａｔｉｏｎａｎｄＩｎｆｏｒｍａｔｉｏｎＴｈｅｏｒｙｉｎＵｎｄｅｒｓｔａｎｄｉｎｇＬａｎｄｓｃ...     

景观生态学中的文化研究

景观生态学中的文化研究李团胜（中国科学院沈阳应用生态研究所,１１００１５）ＲｅｓｅａｒｃｈｏｎＣｕｌｔｕｒｅｉｎＬａｎｄｓｃａｐｅＥｃｏｌｏｇｙ．ＬｉＴｕａｎｓｈｅｎｇ（ＩｎｓｔｉｔｕｔｅｏｆＡｐｐｌｉｅｄＥｃｏｌｏｇｙ,Ａ－ｃａｄｅｍｉａＳｉｎｉｃ...     

A quantitative study of the epiphytic vegetation on pollarded trunks of Fraxinus excelsior at Havrå, Osterøy,western Norway

The epiphytic vegetation on 19 pollarded trees of Fraxinus excelsior at the farm Havrå, Osterøy, western Norway was investigated. Each trunk was divided into a basal zone, a middle zone and a top zone. In each zone the four different aspects were analysed by squares (12 sampling units from each trunk). Within a total of 225 sampling units, 173 taxa were recorded (84 lichens, 72 bryophytes, 17 vascular plants). The epiphytic vegetation was classified into seven TWINSPAN groups, and it is shown by detrended correspondence analysis (DCA) that these groups are mainly separated by the first two DCA axes. In an attempt to explain the floristic composition, several environmental variables were measured, and the floristic and environmental data were analysed by canonical correspondence analysis (CCA). A pollarded tree, depending on its age, represents a continuum of perhaps several hundred years. Some of the recorded epiphytic lichens are very rare in Norway, and the pollarded trees thus contribute greatly to the biodiversity of the old cultural landscape. Several epiphytic species take advantage of favourable light conditions on trunks in the open fields. At Havrå, woodland has developed by tree colonization of old meadows and open fields during the last 40 years after the cessation of earlier managements. Some floristic differences between the epiphytic vegetation on trunks in the open fields and in the woodland are found. This suggests a change in the epiphytic vegetation because of the creation of a more shady habitat in parts of the area during a few decades.     

Impact of a natural enemy overwintering refuge and its interaction with the surrounding landscape

1. Egg parasitoids in the genus Anagrus (Hymenoptera: Mymaridae) are important mortality factors for grape leafhoppers ( Erythroneura elegantula ; Homoptera: Cicadellidae) in California vineyards, yet must overwinter in habitats external to these vineyards. Existing evidence suggests that French prune trees, which harbour the overwintering host Edwardsiana prunicola , planted adjacent to vineyards may enhance early-season abundance of Anagrus.
2. Anagrus overwintering in French prune tree refuges were labelled with the trace element rubidium in four separate experiments. Rubidium-labelled Anagrus were captured in adjacent vineyards in two of the experiments, confirming that French prune trees contribute to early-season Anagrus populations. Anagrus from refuges were captured at the most distant sampling positions, 100 m from refuges.
3. Use of rare element labelling has, for the first time, enabled the relative contribution of different sources to early-season colonization by this parasitoid to be quantified. Refuges contributed 1% and 34% of Anagrus colonizing two of the experimental vineyards, respectively. The remainder originated from overwintering habitats external to the French prune/vineyard system.
4. The spatial patterns of Anagrus originating from external overwintering habitats suggest that the French prune trees are generating a 'windbreak effect'. Anagrus dispersing within the windstream colonized vineyards at a higher-than-average rate immediately downwind of refuges.
5. The amount of colonization by Anagrus from external overwintering habitats was apparently related to the distance to presumed overwintering habitats. These findings demonstrate that both the number of natural enemies emerging from a refuge and the composition of the surrounding landscape are important in determining the impact of local, small-scale habitat manipulations.