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71.
Experimentally induced lesions of cutaneous leishmaniasis and the effect of concurrent bacterial infection on the development of these lesions were studied in the golden hamster. Male outbred golden hamsters received intradermal injections at the base of the tail with approximately 10(7) promastigotes of Leishmania braziliensis panamensis, or promastigotes combined with Staphylococcus aureus or Pasteurella multocida or both, bacteria only, or sterile Eagle's minimal essential medium (MEME). The size of the resulting lesions was measured at least twice each week. Hamsters were killed at postinoculation Days 6, 13, 20, 27, 41, or 48, and each lesion was measured, aseptically excised, and bisected; half was used for bacteriologic culture and the other half was prepared for light microscopic examination. Lesions resulting from L. b. panamensis alone progressed from initial erythema to a granulomatous nodule and finally to a necrotic granuloma, often capped by a crateriform ulcer. Lesions resulting from a suspension of L. b. panamensis with added S. aureus or S. aureus and P. multocida, were initially larger, more erythemic and contained a greater proportion of neutrophils up to postinoculation Days 14-21 than did lesions resulting from L. b. panamensis alone. Concurrent infections with bacteria such as S. aureus and P. multocida had little effect on the development of ulcerating characteristics of lesions, but when S. aureus was present it appeared to enhance the severity of the early lesions. Between postinoculation Days 14-28, lesions produced by L. b. panamensis, with or without added bacteria had similar developmental progression of sufficient size for optimal testing of antileishmanial compounds. 相似文献
72.
When the conditions for detecting proteins by ammoniacal silver staining (B. R. Oakley, D. R. Kirsch, and N. R. Morris (1980) Anal. Biochem. 105, 361-363.) following gel electrophoresis were varied, it was noted that glutaraldehyde pretreatment was necessary for maximal staining, which could not be explained simply as the result of "fixation." Further studies indicated that glutaraldehyde enhancement of protein staining with this silver reagent was probably due to oxidation of the aldehyde groups by silver ions, resulting in metallic silver depositions within the gel which act as nucleation sites for additional metallic silver localization in the protein bands upon the addition of formaldehyde developer. This proposed mechanism is consistent with the Tollen's reaction, as well as some aspects of the photographic process. Consistent with this notion, silver-staining intensities are directly related to mole percentage lysine of various standard proteins. 相似文献
73.
A late Wenlock flora from Co. Tipperary, Ireland 总被引:1,自引:0,他引:1
D. EDWARDS F.L.S. J. FEEHAN D. G. SMITH 《Botanical journal of the Linnean Society. Linnean Society of London》1983,86(1-2):19-36
An assemblage of macroplants preserved as highly coalified compressions which lack anatomy is described from a Wenlock locality in County Tipperary, Ireland. Most of the fertile specimens are assigned to Cooksonia Lang. The taxonomic status of this genus is discussed. Some poorly preserved palynomorphs, including miospores, acritarchs, chitinozoans and a variety of tubes, have been isolated from associated sediments, but the age of the flora is based on graptolites. Sedimentological and palaeontological studies of the region are summarized. They provide little direct evidence for the habitats of the plants which are considered to have been terrestrial. The relevance of this flora to the current debate on the colonization of the land is evaluated and it is concluded that these plants provide the earliest record of erect fertile land plants of possible pteridophyte affinity. 相似文献
74.
数量遗传学中一种新的求综合性状的方法 总被引:4,自引:1,他引:3
本文运用申农(Shannon)提供的最大熵原理,提出一种构成单一综合性状的新方法,并以此与数量遗传学中的多元统计法作了比较。在作多元遗传分析吋,常用多元统计法求出多个数量性状的综合性状,再对这些相互关联的基本性状作主成份分析或用典范相关进行遗传分析。本文提出了不同于多元统计学的另一种新的方法——最大熵法求出多个数量性状的单一综合性状值。它具有数学结构简单,过程明晰,结果简明等优点。 相似文献
75.
用植物试管玻片培养技术研究NH_4~ 对细枝木麻黄及Frankia菌株Co01共生体系建立过程的影响。NH_4~ (100,150ppm(NH_4)_2SO_4)通过阻止菌株Cc01与其宿主细枝木麻黄根毛壁的亲和作用来影响结瘤。但NH_4~ 不能阻抑菌株Cc01中结瘤基因pel和cel的表达及纤维素酶和果胶酶活性,且菌丝一旦侵入宿主皮层细胞,并形成根瘤原基及前根瘤,则NH_4~ (250ppm(NH_4)_2SO_4)就不再阻止原基进一步发育为成熟的根瘤。但在这种情况下,NH_4~ 能抑制根瘤的固氮活性。 相似文献
76.
77.
Second virial coefficient of alpha-crystallin 总被引:1,自引:0,他引:1
Light scattering studies were performed on bovine alpha-crystallin measuring the scattering intensities as a function of scattering angle, concentration, and temperature. The data yielded the molecular weight, radius of gyration, and second virial coefficient of alpha-crystallin at different temperatures. The second virial coefficient increased with increasing temperature. Both the enthalpy and entropy of solution of alpha-crystallin are positive. The Flory theta temperature was found to be 271 K. 相似文献
78.
Seroprevalence of B virus (Herpesvirus simiae) antibodies in a naturally formed group of rhesus macaques 总被引:2,自引:0,他引:2
Eighty-two percent of a group of rhesus monkeys removed from Cayo Santiago were seropositive for B virus (Herpesvirus simiae) antibodies. Similar results were obtained from the Cayo Santiago macaque population two decades ago and from feral Indian rhesus monkeys. Thus it is likely that B virus has been enzootic in the Cayo Santiago population since 1938, when the colony was established with stock imported from India. 相似文献
79.
Paiva R. P. Munita C. J. S. Cunha I. I. L. Romano J. Alonso C. D. 《Biological trace element research》1990,(1):231-242
Two nuclear techniques, Energy-Dispersive X-Ray Fluorescence Analysis (EDXRF) and Instrumental Neutron Activation Analysis (INAA), were used to analyze aerosol samples collected in the city of São Paulo, Brazil. Na, Cl, Mn, V, Al, Sm, Mo, W, La, As, Br, Sb, K, Ba, Se, Th, Cr, Rb, Ca, Fe, Ce, and Sc were determined by INAA, and Al, Si, P, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, Ga, As, Se, Br, Rb, Sr, Hg, and Pb were determined by EDXRF. A preliminary identification of the main source of the atmospheric aerosol was performed based on enrichment factor and correlation coefficient calculations.
相似文献80.
试论云阳县人口激增与土地锐减的矛盾及对策 总被引:2,自引:0,他引:2
土地是人们赖以生存的物质基础,是其它任何东西不可代替的生产资料。离开了土地,人就不能生存下去,这是众所周知的真理。但是,目前人口的激增与土地锐减的矛盾越来越尖锐。据有关统计资料表明,1978—1988年全国耕地面积减少3.66×10~6ha,与此同时全国人口又猛增1.33亿,给农业粮食生产、 相似文献