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991.
The phenolic glycosides salicin and salicortin were found to influence larval growth and development rates and adult feeding preference of Phratora vulgatissima in laboratory feeding studies. Salicortin was more toxic to larvae than salicin, and none of the third instar larvae fed on Salix viminalis leaves amended with 1.52% (fresh mass) salicortin pupated. Condensed tannins (proanthocyanadins) did not affect larval performance. It was concluded that Salix burjatica resistance to willow beetle is due to the high levels of salicortin which occur in leaves of this species. 相似文献
992.
993.
Summary Regulation of ion-channel activity must take place in order to regulate ion transport. In case of tonoplast ion channels, this is possible on both the cytoplasmic and the vacuolar side. Isolated vacuoles of youngVigna unguiculata seedlings show no or hardly any channel activity at tonoplast potentials >80 mV, in the vacuole-attached configuration. When the configuration is changed to an excised patch or whole vacuole, a fast (excised patch) or slow (whole vacuole) increase of inward rectifying channel activity is seen. This increase is accompanied by a shift in the voltage-dependent gating to less hyperpolarized potentials. In the whole vacuole configuration the level of inward current increases and also the activation kinetics changes. Induction of channel activity takes up to 20 min depending on the age of the plants used and the diameter of the vacuole. On the basis of the estimated diffusion velocities, it is hypothesized that a compound with a mol wt of 20,000 to 200,000 is present in vacuoles of young seedlings, which shifts the population of channels to a less voltage-sensitive state.Ecotrans publication no. 27. 相似文献
994.
Françoise Simon-Plas Kees Venema Jean-Pierre Grouzis Rémy Gibrat Jacqueline Rigaud Claude Grignon 《The Journal of membrane biology》1991,120(1):51-58
Summary The purified (H+ATPase from corn roots plasma membrane inserted spontaneously into preformed bilayer from soybean lipids. The yield of the protein insertion, as measured from its H+-pumping activity, increased as a function of lipids and protein concentrations. In optimum conditions, all the (H+)ATPase molecules were closely associated with liposomes, exhibiting a high H+-pumping activity (150,000% quenching· min–1·mg–1 protein of the probe 9-amino-6-chloro-2-methoxyacridine). The insertion was achieved within a few seconds. No latency of the (H+)ATPase hydrolytic activity was revealed when lysophosphatidylcholine was added to permeabilize the vesicles. This indicated that the (H+)ATPase molecules inserted unidirectionally, the catalytic sites being exposed outside the vesicles (inside-out orientation), and thus freely accessible to Mg-ATP. The nondelipidated (H+)ATPase could also functionally insert into bilayer from PCPEPG or PCPEPI, due to the presence of both hydrophobic defects promoted by PE, and negative phospholipids specifically required by the (H+)ATPase from corn roots. The detergent octylglucoside facilitated the delipidated (H+)ATPase reinsertion probably by promoting both a proper protein conformation and hydrophobic defects in the bilayer. Lysophosphatidylcholine facilitated the delipidated protein insertion only when hydrophobic defects were already present, and thus seemed only capable to ensure a proper protein conformation 相似文献
995.
Intragenic Sequences Affect the Expression of the Gene Encoding Glial Fibrillary Acidic Protein 总被引:6,自引:1,他引:5
We show that the expression of the gene encoding glial fibrillary acidic protein (GFAP) gene is affected by at least three cis-acting elements. A positive regulatory element that is located between nucleotides -1,631 and -1,479 can confer cell type-specific expression on a heterologous gene. A second regulatory element is located between nucleotides -97 and -80. The third is a negative regulatory element that is located within the first intron of the gene. Deletion of this element activates GFAP expression in HeLa cells, and affects promoter function in glioma cells. 相似文献
996.
An improved approach for transformation of plant cells by microinjection: molecular and genetic analysis 总被引:4,自引:0,他引:4
Martin Schnorf Gabriele Neuhaus-Url Alessandro Galli Shigeru Iida Ingo Potrykus Gunther Neuhaus 《Transgenic research》1991,1(1):23-30
A new culture method for the injection of tobacco mesophyll protoplasts has been established. The protoplasts are embedded
in a thin layer of alginate and are nourished from the medium in the underlying basislayer. In the alginate layer the protoplasts
regenerate to calli at a frequency of up to 80%. Embedded protoplasts can be selected either with 50 mg l−1 kanamycin or 5 mg l−1 paromomycin. Single resistant cells can be recovered from about 10 000 sensitive cells in one alginate layer. Injection of
theneo gene (coding for neomycin phosphotransferase II) into protoplast derived single cells in the alginate layer results in kanamycin
resistant colonies that can be regenerated to mature plants. These plants express the neomycin phosphotransferase as shown
by enzyme activity assay. The integration of the transgene into the plant genome could be proved by Southern hybridization
to high molecular weight DNA. With this culture method 100 cells can be injected per hour. Transformation frequencies range
from 2 to 20%. In crossing experiments, it was shown that the foreign gene is transmitted to the next generation in a Mendelian
fashion. 相似文献
997.
鄂东花马湖水生高等植物研究:Ⅰ.水生植物区系 总被引:3,自引:1,他引:2
冯灿;李鸿钧;於炳;王业华;李胜荣 《武汉植物学研究》1991,9(4):363-371
花马湖位于北纬30°15′—30°18′、东经114°58′—115°1′,面积约27.5km~2。湖中分布82种2变种水生维管束植物,隶属于34科66属,其中资源植物众多。花马湖水生高等植物大型属少,小型属较多,单型属相对突出。区系地理成分以温带成分占优势,计34种,占全湖种类的40.48%。起源古老的成分也较多。通过与有关湖泊水生植物区系的比较,及区系成分的分析,说明花马湖水生植物区系具有从暖温带至中亚热带过渡的特点。 相似文献
998.
刺槐(Robinia pseudoacacia)生长迅速,适应性强,耐干旱,树冠大郁闭早,枯枝落叶多易分解,改良土壤的作用明显,已成为陕西渭北地区的主要造林树种。多年来,许多学者的研究表明,森林凋落 相似文献
999.
中国红树植物营养器官的结构与生态适应的研究:Ⅲ 茎 总被引:9,自引:0,他引:9
本文主要报道分布在我国热带、亚热带海岸的10科13属17种红树植物,在特殊的生态环下,茎的形态结构特征与趋同适应。阐明生境对植物形态结构的密切影响。 相似文献
1000.