全文获取类型
收费全文 | 4510篇 |
免费 | 426篇 |
国内免费 | 369篇 |
出版年
2023年 | 100篇 |
2022年 | 53篇 |
2021年 | 152篇 |
2020年 | 174篇 |
2019年 | 179篇 |
2018年 | 134篇 |
2017年 | 124篇 |
2016年 | 156篇 |
2015年 | 193篇 |
2014年 | 179篇 |
2013年 | 282篇 |
2012年 | 191篇 |
2011年 | 192篇 |
2010年 | 189篇 |
2009年 | 192篇 |
2008年 | 211篇 |
2007年 | 218篇 |
2006年 | 200篇 |
2005年 | 188篇 |
2004年 | 156篇 |
2003年 | 176篇 |
2002年 | 177篇 |
2001年 | 166篇 |
2000年 | 113篇 |
1999年 | 113篇 |
1998年 | 95篇 |
1997年 | 101篇 |
1996年 | 98篇 |
1995年 | 86篇 |
1994年 | 88篇 |
1993年 | 59篇 |
1992年 | 63篇 |
1991年 | 62篇 |
1990年 | 22篇 |
1989年 | 33篇 |
1988年 | 33篇 |
1987年 | 34篇 |
1986年 | 28篇 |
1985年 | 52篇 |
1984年 | 42篇 |
1983年 | 33篇 |
1982年 | 35篇 |
1981年 | 23篇 |
1980年 | 25篇 |
1979年 | 17篇 |
1978年 | 15篇 |
1977年 | 10篇 |
1976年 | 10篇 |
1975年 | 7篇 |
1973年 | 11篇 |
排序方式: 共有5305条查询结果,搜索用时 15 毫秒
71.
Studies were undertaken on interactions between panicle size, insect density, host plant, and the environment for sorghum
head bug,Calocoris angustatus Lethiery on five sorghum genotypes in terms of bug population increase, grain damage and loss in grain mass across four panicle
sizes (5, 10 or 20 branches/panicle and whole panicle), and three infestation levels (5, 10 and 15 pairs of bugs/panicle).
Head bug numbers increased and grain damage decreased with an increase in panicle size in the head bug susceptible cultivars,
CSH 1 and CSH 5. However, the increase in bug numbers or decrease in grain damage was not significant in head bug resistant
genotypes, IS 17610 and IS 17645. Head bug numbers increased with an increase in infestation level in CSH 1 and CSH 5, however,
such an increase was not substantial in IS 17610 and IS 17645. Grain damage was significantly lower in IS 17610 and IS 17645
compared with CSH 1 and CSH 5 across infestation levels. Head bug population increased at a greater rate during the rainy
season compared with the dry season. Panicle size and infestation levels accounted for greater variation in grain damage and
percentage loss in grain mass during the rainy season than in the dry season. To identify reliable sources of resistance to
insects, it is important to study insect host plant-interactions across panicle sizes (levels of food availability), infestation
levels and seasons. 相似文献
72.
The effects of elevated atmospheric CO2 concentration on plant-fungi and plant-insect interactions were studied in an emergent marsh in the Chesapeake Bay. Stands of the C3 sedge Scirpus olneyi Grey, and the C4 grass Spartina patens (Ait.) Muhl. have been exposed to elevated atmospheric CO2 concentrations during each growing season since 1987. In August 1991 the severities of fungal infections and insect infestations were quantified. Shoot nitrogen concentration ([N]) and water content (WC) were determined. In elevated concentrations of atmospheric CO2, 32% fewer S. olneyi plants were infested by insects, and there was a 37% reduction in the severity of a pathogenic fungal infection, compared with plants grown in ambient CO2 concentrations. S. olneyi also had reduced [N], which correlated positively with the severities of fungal infections and insect infestations. Conversely, S. patens had increased WC but unchanged [N] in elevated concentrations of atmospheric CO2 and the severity of fungal infection increased. Elevated atmospheric CO2 concentration increased or decreased the severity of fungal infection depending on at least two interacting factors, [N] and WC; but it did not change the number of plants that were infected with fungi. In contrast, the major results for insects were that the number of plants infected with insects decreased, and that the amount of tissue that each insect ate also decreased. 相似文献
73.
J. Trémouillaux-Guiller H. Kodja J. C. Chénieux 《Plant Cell, Tissue and Organ Culture》1994,37(1):25-30
Protoplasts enzymatically isolated from cell line of Catharanthus roseus G. Don crown gall, were cultured at high density (105 P ml-1) in modified B5 liquid medium (Gamborg et al. 1976). In the absence of growth regulators C. roseus protoplasts were able to regenerate a cell-wall, divide and, subsequently, yield very numerous clones in the absence of growth regulators. After two weeks, the cultures were greatly diluted in order to obtain clones of single-cell origin. Most of the clones individually transferred onto solid medium can proliferate indefinitely, without growth regulators. Among analyzed clones, 90% were nopaline positive. Their ajmalicine and serpentine content was compared with that of the parental crown gall line, and was found to be low. The CR10 protoplasts were very easy to grow, they were an interesting model for the development of pure tumorous lines. Moreover, we found that the tumorous protoplasts were useful for cell fusion experiments or for the delicate culture of tree protoplasts.Abbreviations B5
Gamborg et al. (1976) medium
- 2,4-d
2,4-dichlorophenoxyacetic acid
- Kin
Kinetin
- NAA
naphthalene acetic acid
- BA
N6 (benzyl) adenine 相似文献
74.
The root-knot nematode Meloidogyne incognita was cultured monoxenically on excised tomato roots. Galls and egg masses were observed daily using a light microscope. Two phases were distinguished in the gelatinous matrix of the egg mass: a translucent, amorphous material on the surface of the egg mass and a denser, layered phase in which nematode eggs were deposited. Egg masses were also cryofixed, fractured, and observed as frozen, hydrated specimens on a cold stage in a scanning electron microscope (SEM). In the SEM, the layered phase appeared as a meshwork of fibrils that became more loosely associated as the gelatinous matrix aged: Small pearl-like bodies were observed along the fibers of gelatinous matrix. The egg shell surface and several stages of embryo development, including the one-cell stage, initial cleavages, blastula, gastrula, tadpole stage, elongation, and molt of the first-stage juvenile within the egg shell, were observed and photographed with this technique. The developmental events observed were consistent with those described in other nematode species with different techniques. 相似文献
75.
The phospholipid composition of Steinernema carpocapsae was studied in relation to diet and culture temperature. When reared at 18 and 27.5 C on Galleria mellonella or on an artificial diet supplemented with lard, linseed oil, or fish oil as lipid sources, nematode phospholipids contained an abundance of 20-carbon polyunsaturated fatty acids, with eicosapentaenoic acid (20:5(n - 3)) predominant, regardless of the fatty acid composition of the diet. Because the level of linolenic acid (18:3(n - 3)) in nematode phospholipids was very low and because eicosapentaenoic acid was present even when its precursor (linolenic acid) was undetectable in the diet, S. carpocapsae likely produces n - 3 polyunsaturated fatty acids by de novo biosynthesis, a pathway seldom reported in eukaryotic animals. Reduction of growth temperature from 25 to 18 C increased the proportion of 20:5(n - 3) but not other polyunsaturated fatty acids. A fluorescence polarization technique revealed that vesicles produced from phospholipids of nematodes reared at 18 C were less ordered than those from nematodes reared at 27.5 C, especially in the outermost region of the bilayer. Dietary fish oil increased fluidity in the outermost region but increased rigidity in deeper regions. Therefore, S. carpocapsae appears to modify its membrane physical state in response to temperature, and eicosapentaenoic acid may be involved in this response. The results also indicate that nematode membrane physical state can be modified dietarily, possibly to the benefit of host-finding or survival of S. carpocapsae at low temperatures. 相似文献
76.
Pepper plants were grown under different water and nitrogen availabilities that produced severe nitrogen limitations and mild water stress. Nitrogen limitation produced lower leaf N content, higher C:N, and higher leaf content of phenolic compounds, in consonance with the carbon/nutrient balance hypothesis. Nitrogen limitation also produced lower nutritional quality of leaves, with lower relative growth rates and lower efficiency of conversion of ingested biomass on the polyphagous herbivoreHelicoverpa armigera. The biomass gained per gram nitrogen ingested also tended to be lower in those insects feeding on nitrogen-limited plants, in parallel with their higher phenolic content. However, larvae fed on nitrogen-limited plants did not increase the ingestion of food to compensate for the N deficiency of leaves. The mild water stress, which only slightly tended to increase the phenolic content of pepper leaves, had no significant effect on nutritional indices. 相似文献
77.
R. Peñalver B. Vicedo C. I. Salcedo M. M. López 《Biocontrol Science and Technology》1994,4(3):259-267
Agrobacterium radiobacter strains K84, K1026 and K84 Agr‐ produced in vitro an antibiotic‐like substance (ALS 84), different from agrocin 84 and observed in mannitol‐glutamate medium. Twenty five out of 39 A. tumefaciens strains of biovars 1, 2 and 3 were sensitive to ALS 84 regardless of their sensitivity to agrocin 84. Sensitivity in A. tumefaciens strain C58 was not encoded by the Ti‐plasmid. Most isolates tested of Erwinia carotovora subsp. carotovora E. carotovora subsp. atroseptica, Pseudomonas corrugata P. cichorii and unidentified isolates from galls were also sensitive to this substance. ALS 84 was not affected by the proteases studied, nor by treatment at 62°C for 30 min and had a bacteriostatic effect. The production of ALS 84 might play a role in the complex mechanism of biological control of crown gall, especially in strains resistant to agrocin 84 and sensitive to ALS 84, and by the creation of an ecological niche favourable to A. radiobacter strains K84, K1026 or K84 Agr‐. 相似文献
78.
U. Bässler U. Nothof 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1994,175(1):23-33
In the artificially closed femur-tibia control system of stick insects oscillations were induced in 3 different ways: Increasing the phase-shift by introducing an electronic delay, afference sign reversal and coupling the tibia to an inert mass. In all 3 cases the oscillations stopped after some time. The gain of the open-loop system was significantly smaller after the oscillations. Afference sign reversal by surgically crossing of the receptor apodeme of the femoral chordotonal organ for 25–85 days does not lead to altered characteristics of the control loop. When sinusoidal passive movements are forced upon the intact femur-tibia joint the forces resisting these movements do not decrease with time. In contrast to direct stimulation of the femoral chordotonal organ, these passive movements also influence the contralateral leg. The experiments show that the gain-control system of the femur-tibia control loop of stick insects consists of at least two components: A sensitization system (with inputs from many kinds of stimuli indicating some kind of disturbance) increases the gain of all reflex loops. A specific habituation-like system decreases the gain with repetitive stimulation only of one control system.Abbreviations fCO
femoral chordotonal organ
- SETi
slow extensor tibiae motor neuron 相似文献
79.
樟脊网蝽生物学特性观察 总被引:2,自引:0,他引:2
樟脊网蝽是香樟的重要害虫,在上海1年发生4代。4月下旬越冬卵始孵,9月下旬开始出现越冬卵。成虫末见期在11月中旬。经室内饲养,樟脊网蝽第三代各龄若虫历期为:一龄61.45±7.8h,二龄48.00±7.6h,三龄43.43±7.6h,四龄51.00±8.0h,五龄70.50±7.5h,整个若虫期历期为281.92±14.8h。第三代野外雌雄比为1:4.07。第三代每雌产2~96粒。日最高产卵量为34粒/头,孤雌可产卵。10%灭百可2000倍稀释液和50%杀螟松2000倍稀释液喷雾防治效果可达93%以上。 相似文献
80.
Patricia Jurez 《Archives of insect biochemistry and physiology》1994,25(3):177-191
A new approach to insect control—using sodium trichloroacetate (NaTCA) to inhibit synthesis of the hydrophobic cuticular lipids that protect insects from dehydration—was tested on Triatoma infestans. In vivo and in vitro studies of incorporation of radioactive precursors showed diminished cuticular hydrocarbon synthesis after NaTCA treatment. Thin layer chromatography and scanning electron microscopy showed disruption of the cuticular lipid layer of NaTCA-treated insects, which also have increased mortality and altered molting cycles. NaTCA treatment enhanced the penetration and increased the lethality of a contact insecticide. © 1994 Wiley-Liss, Inc. 相似文献