全文获取类型
收费全文 | 32245篇 |
免费 | 2559篇 |
国内免费 | 2507篇 |
出版年
2024年 | 100篇 |
2023年 | 766篇 |
2022年 | 723篇 |
2021年 | 1290篇 |
2020年 | 1306篇 |
2019年 | 1529篇 |
2018年 | 1306篇 |
2017年 | 1159篇 |
2016年 | 1205篇 |
2015年 | 1510篇 |
2014年 | 1908篇 |
2013年 | 2839篇 |
2012年 | 1379篇 |
2011年 | 1616篇 |
2010年 | 1134篇 |
2009年 | 1710篇 |
2008年 | 1786篇 |
2007年 | 1737篇 |
2006年 | 1559篇 |
2005年 | 1309篇 |
2004年 | 1183篇 |
2003年 | 1016篇 |
2002年 | 823篇 |
2001年 | 689篇 |
2000年 | 620篇 |
1999年 | 573篇 |
1998年 | 542篇 |
1997年 | 513篇 |
1996年 | 411篇 |
1995年 | 373篇 |
1994年 | 309篇 |
1993年 | 318篇 |
1992年 | 271篇 |
1991年 | 254篇 |
1990年 | 216篇 |
1989年 | 183篇 |
1988年 | 155篇 |
1987年 | 128篇 |
1986年 | 125篇 |
1985年 | 144篇 |
1984年 | 118篇 |
1983年 | 73篇 |
1982年 | 126篇 |
1981年 | 78篇 |
1980年 | 62篇 |
1979年 | 42篇 |
1978年 | 28篇 |
1977年 | 15篇 |
1976年 | 13篇 |
1974年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
Sodium-induced calcium deficiency in salt-stressed corn 总被引:9,自引:5,他引:4
Abstract The effect of the Na+/Ca2+ ratio in the root media on salt-stressed corn (Zea mays L. cvs DeKalb XL-75 and Pioneer 3906) was determined in greenhouse experiments. Plants grown in a complete nutrient solution salinized with 86.5 mol m?3 NaCl exhibited severe Ca2+ deficiency symptoms at the four-leaf stage. The symptoms disappeared when part of the NaCl was replaced with 10 mol m?3 CaCl2 (Na+/Ca2+ molar ratio = 5.7). Salt stress at an iso-osmotic potential of ?0.4 MPa substantially decreased shoot growth at all solution Na+/Ca2+ ratios from 34.6 to 0.26. However, the dry weights of blades at 26 d of age were much less when plants were salinized with NaCl alone, particularly that of DeKalb XL-75 which was more susceptible to Na-induced Ca2+ deficiency than was Pioneer 3906. The growth of sheaths was similarity reduced by sail stress at all Na+/Ca2+ ratios. The symptoms of Ca2+ deficiency were correlated with low Ca2+ concentrations in the leaf tissue. Ca2+ concentrations in the developing blades of NaCl-stressed plants were much lower than in control plants. As the Na+/Ca2+ ratio in the solution was decreased, Ca2+ levels increased in both the blades and sheaths while Na+ concentrations greatly decreased. DeKalb XL-75 was much less effective than Pioneer 3906 in restricting the uptake of Na+. The results clearly indicate that NaCl stress may cause lesions and unique plant responses that are not manifested on agronomic plants grown on saline soils. 相似文献
82.
Early stomatal closure in waterlogged pea plants is mediated by abscisic acid in the absence of foliar water deficits 总被引:10,自引:2,他引:8
Abstract Soil waterlogging decreased leaf conductance (interpreted as stomatal closure) of vegetative pea plants (Pisuin sativum L. cv. ‘Sprite’) approximately 24 h after the start of flooding, i.e. from the beginning of the second 16 h-long photo-period. Both adaxial and abaxial surfaces of leaves of various ages and the stipules were affected. Stomatal closure was sustained for at least 3 d with no decrease in foliar hydration measured as water content per unit area, leaf water potential or leaf water saturation deficit. Instead, leaves became increasingly hydrated in association with slower transpiration. These changes in the waterlogged plants over 3 d were accompanied by up to 10-fold increases in the concentration of endogenous abscisic acid (ABA). Waterlogging also increased foliar hydration and ABA concentrations in the dark. Leaves detached from non-waterlogged plants and maintained in vials of water for up to 3 d behaved in a similar way to leaves on flooded plants, i.e. stomata closed in the absence of a water deficit but in association with increased ABA content. Applying ABA through the transpiration stream to freshly detached leaflets partially closed stomata within 15 min. The extractable concentrations of ABA associated with this closure were similar to those found in flooded plants. When an ABA-deficient ‘wilty’ mutant of pea was waterlogged, the extent of stomatal closure was less pronounced than that in ordinary non-mutant plants, and the associated increase in foliar ABA was correspondingly smaller. Similarly, waterlogging closed stomata of tomato plants within 24 h, but no such closure was seen in ‘flacca’, a corresponding ABA-deficient mutant. The results provide an example of stomatal closure brought about by stress in the root environment in the absence of water deficiency. The correlative factor operating between the roots and shoots appeared to be an inhibition of ABA transport out of the shoots of flooded plants, causing the hormone to accumulate in the leaves. 相似文献
83.
David B. Collinge Dawn E. Milligan J. Maxwell Dow Graham Scofield Michael J. Daniels 《Plant molecular biology》1987,8(5):405-414
Xanthomonas campestris pv. vitians, a pathogen of lettuce, elicits a hypersensitive response within 12 hours of inoculation into Brassica leaves, characterized by tissue collapse, loss of membrane integrity, vein blockage and melanin production. In contrast, the compatible pathogen, X. c. pv. campestris, has no visible effects on leaves for 48 hours, after which inoculated areas show chlorosis which eventually spreads, followed by rotting.mRNA was prepared from leaves inoculated with suspensions of both pathovars or with sterile medium up to 24 hours following inoculation. In vitro translation of total and poly A+ RNA in rabbit reticulocyte lysate in the presence of 35S methionine followed by separation of the polypeptide products by 2D-PAGE, allowed comparison of the effects of these treatments on plant gene expression. Major changes in gene expression were observed as a consequence of the inoculation technique. In addition, after inoculation with X. c. vitians, up to fifteen additional major polypeptides appeared or greatly increased by four hours. Some of these had disappeared by nine hours and several more had appeared. No major polypeptides disappeared or decreased greatly in intensity following inoculation with X. c. vitians. 相似文献
84.
The plant growth regulators, gibberellic acid (GA3), ethephon and chlormequat chloride (CCC) were sprayed on young lettuce, cauliflower and bean (Phaseolus vulgaris) plants, which had either been given or not been given a mechanically-induced stress (MIS) treatment. MIS was applied by brushing the plants with paper for 1.5 minutes each day. GA3 increased extension growth of bean and leaf length of lettuce in unbrushed plants as much as in brushed ones. CCC and ethephon were less effective at reducing the height of brushed bean plants compared to unbrushed ones. The effects of CCC on the growth of cauliflower and lettuce plants was not influenced by brushing, whereas unbrushed plants responded more readily to ethephon than did brushed ones. The effects of CCC on growth were generally similar to those of MIS whereas the effects of ethephon were in many ways different to MIS.The results are discussed in relation to the use of PGR and MIS treatments for modifying plant growth. 相似文献
85.
M. Agami 《Oecologia》1987,71(4):486-490
Summary The hills of the Negev highlands, west of Sede Boqer, are typically covered by various half-shrub communities, including the deciduous species of Helianthemum vesicarium Boiss., an Irano-Turanian element that grows only on north facing slopes, and H. ventosum Boiss., a Saharo-Arabian element which grows on both slopes but mainly on those facing south. Upon irrigation, plants of H. vesicarium preserve their natural rhythm of activity during winter and remain deciduous throughout the summer. On the other hand, under a similar irrigation treatment, the growth and flowering season of H. ventosum is modified and is extended well into the summer. Thus, H. ventosum shows a high phenological plasticity, while H. vesicarium seems to be very conservative. The different response of the two species to an improved water regime may partly explain the differences in their natural habitats: H. vesicarium on the more humid north facing slopes and H. ventosum mostly on the more arid and extreme south facing ones. Plants of both species which grow near boulders attain larger sizes and denser stands than those on the slopes.Age analysis based on xylem ring counts of 859 plants of the two natural populations showed that the plants did not exceed 14 years and most of them reached the age of some 5 years only. In general more seedlings are established during rainy year-clusters than during dry ones. However, no direct correlation between seedling establishment and the annual rainfall of specific years could be found.The rapid turnover of plants in the stands of both species of Helianthemum resembles the behavior of desert annuals rather than that of most desert shrubs. 相似文献
86.
Cultures of the unicellular green alga Chlamydomonas reinhardii can be synchronized by light/dark cycling not only under photoautotrophic but also under mixotrophic growth conditions. We observed that cultures synchronized in the presence of acetate continue to divide synchronously for one cell-cycle period when transferred to heterotrophic growth conditions. This finding enabled us to investigate the differential effects of light on cell growth and cell division. When cells were exposed to continuous light at the beginning of the growth period they entered the division phase earlier than dark-grown cells as a consequence of an increased growth rate. Illumination at the end of the growth period, however, caused a considerable delay in cell division and an extended growth period. The light-induced delay in cell division was also observed in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), an inhibitor of photosystem II. This finding demonstrates that cell division is directly influenced by a light/dard-responsive cell-cycle switch rather than by light/dark-dependent changes in energy metabolism. The importance of this light/dark control to the regulation of the Chlamydomonas cell cycle was investigated in comparison with other control mechanisms (size control, time control). We found that the light/dard-responsive cell-cycle switch regulates the transition from G1-to S-phase. This control mechanism is effective in cells which have attained the commitment to at least one round of DNA replication and division but have not attained the maximal cell mass which initiates cell division in the light.Abbreviations dCTP
deoxycytidine 5-triphosphate
- DCMU
3-(3,4-dichlorophenyl)-1,1-dimethylurea 相似文献
87.
The quality of Cucurbita pepo L. pollen was studied using field pollinations and the fluorochromatic-reaction test. The extreme sensitivity of this pollen to dehydration and ageing is demonstrated. Controlled stress applied to mature pollen leads to the development of seedless fruits. Molecular signals seem to be involved in the induction of this parthenocarpy. These results indicate the existence of distinct sequences involved in the completion of the fertilization program of pollen. With pollen altered by stress, the fertilization process may be stopped at different stages of its completion. We bring evidence that Cucurbita pepo plants have developed special adaptations in order to compensate for the poor viability of their pollen.Abbreviation FCR
fluorochromatic reaction 相似文献
88.
Day/night changes in turgor pressure (P) and titratable acidity content were investigated in the (Crassulacean-acid-metabolism (CAM) plant Kalanchoe daigremontiana. Measurements of P were made on individual mesophyll cells of intact attached leaves using the pressure-probe technique. Under conditions of high relative humidity, when transpiration rates were minimal, changes in P correlated well with changes in the level of titratable acidity. During the standard 12 h light/12 h dark cycle, maximum turgor pressure (0.15 MPa) occurred at the end of the dark period when the level of titratable acidity was highest (about 300 eq H+·g-1 fresh weight). A close relationship between P and titratable acidity was also seen in leaves exposed to perturbations of the standard light/dark cycle. (The dark period was either prolonged, or else only CO2-free air was supplied in this period). In plants deprived of irrigation for five weeks, diurnal changes in titratable acidity of the leaves were reduced (H=160 eq H+·g-1 fresh weight) and P increased from essentially zero at the end of the light period to 0.02 MPa at the end of the dark period. Following more severe water stress (experiments were made on leaves which had been detached for five weeks), P was zero throughout day and night, yet small diurnal changes in titratable acidity were still measured. These findings are discussed in relation to a hypothesis by Lüttge et al. 1975 (Plant Physiol. 56,613-616) for the role of P in the regulation of acidification/de-acidification cycles of plants exhibiting CAM.Abbreviations CAM
crassulacean acid metabolism
- FW
fresh weight
-
P
turgor pressure 相似文献
89.
Mechanics of root growth 总被引:4,自引:1,他引:3
A. R. Dexter 《Plant and Soil》1987,98(3):303-312
Summary A model is developed for the rate of elongation of a root tip in terms of the balance of pressures acting on the root. Differentials
of this equation give expressions for the changes in root elongation rate with respect to soil water potential and soil mechanical
resistance. The model predicts that root cells osmoregulate against both water stress and soil mechanical resistance with
predicts that root cells osmoregulate against both water stress and soil mechanical resistance with similar efficiencies which
are less than 100%. Analysis of published data leads to the conclusion that root tips of pea osmoregulate with 70% efficiency.
A working equation is developed for the elongation rate of roots in conditions of combined water stress and mechanical resistance. 相似文献
90.
Cladocera in space and time: Analysis of lake sediments 总被引:6,自引:6,他引:0
Wolfgang Hofmann 《Hydrobiologia》1987,145(1):315-321
Shells of Bosminidae and Chydoridae are quantitatively preserved in lake sediments. The chronological deposition of these
remains provides the means for longterm observation of these Cladocera, both in terms of species and communities.
Chydorid analysis, as based on subfossil assemblages, is an analysis of community and provides direct observation of community
dynamics over extended periods of time. It has proved to be a valuable method to obtain information on the influence of environmental
factors and time on community characteristics.
Morphological variation inBosmina (Eubosmina) has been followed for some thousand years. This is of special interest for the evaluation of taxonomic rank (species, forms)
if closely related taxa have co-existed.
Bosmina successions, as well as shifts in the chydorid fauna, are related to environmental change. Thus, cladoceran analysis of lake
sediments provides information on the developmental history of lakes and allows observation of the effects of longterm environmental
changes, such as climatic changes and eutrophication. 相似文献