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161.
Haiyan Liu Heguo Yu Aijie Xin Huijuan Shi Yihua Gu Yonglian Zhang Hua Diao Donghai Lin 《Journal of peptide science》2014,20(4):251-257
Public health of human beings is threatened by superbugs. Novel human beta‐defensins, which contribute to host defense against pathogen invasion and innate immune protection, might be a potent natural candidate pool for new antibiotic lead screening. In the present work, we successfully expressed and purified a novel human beta‐defensin, DEFB120, using the IMPACT‐TWIN system in Escherichia coli and identified the purified homogeneous proteins using MALDI‐TOF mass spectrometry. Then, we performed the fundamental studies on the structure and biological functions for the DEFB120 peptide. The recombinant DEFB120 peptide showed wide antimicrobial effects against E. coli, Staphylococcus aureus and Candida albicans strains without significant hemolytic activity. Furthermore, the high lipopolysaccharide (LPS)‐binding affinity in vitro indicated that DEFB120 might be associated with the inhibition of LPS‐induced inflammatory response. These results may pave a way for exploiting the essential physiological functions of DEFB120 and also for the development of natural antibiotic pools. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
162.
Viviane?V. do Nascimento érica de?O. Mello Laís?P. Carvalho Edésio?J.T. de Melo André de?O. Carvalho Katia?V.S. Fernandes Valdirene?M. Gomes 《Bioscience reports》2015,35(5)
Plant defensins are small cysteine-rich peptides and exhibit antimicrobial activity against a variety of both plant and human pathogens. Despite the broad inhibitory activity that plant defensins exhibit against different micro-organisms, little is known about their activity against protozoa. In a previous study, we isolated a plant defensin named PvD1 from Phaseolus vulgaris (cv. Pérola) seeds, which was seen to be deleterious against different yeast cells and filamentous fungi. It exerted its effects by causing an increase in the endogenous production of ROS (reactive oxygen species) and NO (nitric oxide), plasma membrane permeabilization and the inhibition of medium acidification. In the present study, we investigated whether PvD1 could act against the protozoan Leishmania amazonensis. Our results show that, besides inhibiting the proliferation of L. amazonensis promastigotes, the PvD1 defensin was able to cause cytoplasmic fragmentation, formation of multiple cytoplasmic vacuoles and membrane permeabilization in the cells of this organism. Furthermore, we show, for the first time, that PvD1 defensin was located within the L. amazonensis cells, suggesting the existence of a possible intracellular target. 相似文献