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91.
HANS-MARTIN SEYFERT 《The Journal of eukaryotic microbiology》1979,26(1):66-74
SYNOPSIS.
Under the growth conditions employed, the G1 macronucleus of Tetrahymena pyriformis HSM contains 7.4 × 10-12 g DNA, the G2 micronucleus 0.42 × 10-12 g. DNA content from the Tetrahymena thermophila macronucleus did not significantly differ from that of HSM, but the micronucleus contained about twice as much DNA as the micronucleus of the HSM cells. The T. thermophila macronucleus contained on average enough DNA for ˜ 35 haploid micronuclear copies. A new spreading technic allowed separation of macronuclear substructures from cells of late G2 to early G1 . Photometric determination of DNA content of 345 individual structures suggested the existence of 5 different-sized macronuclear structures with a DNA content corresponding to 2, 4, 8, and 16 × the basic values. Comparison of the DNA content of these structures with (a) mitotic micronuclear chromosomes and (b) meiotic micronuclear chromosomes of T. thermophila cells suggests that the 5 basic values of macronuclear structures derive from structures of micronuclear chromosomes. The micronuclear chromosomes of T. pyriformis may be oligotenic. It is suggested that these results further our understanding of macronuclear organization. 相似文献
Under the growth conditions employed, the G
92.
Jayr de Paiva Campello Sebastião Ferreira Fonseca Ching-Jer Chang Ernest Wenkert 《Phytochemistry》1975,14(1):243-248
Sitosterol and the following terpenic compounds have been isolated from the bark of Podocarpus lambertius: 3β-hydroxytotarol, 4β-carboxynortotarol, and macrophyllic and lambertic acids. The leaves yielded sitosterol, stigmastan-3β,5α-diol-6-one, isopimaric acid, phyllocladene, isophyllocladene, 8,9-abieten-15-ol and 17-isophyllocladenol. 相似文献
93.
Two enantiomeric cuparene-type sesquiterpenoids, (R)-(−)-cuparene (1) and (R)-(−)-δ-cuparenol (2), have been isolated from the liverwort, Bazzania pompeana. The structures and absolute configurations of the two compounds have been determined. 相似文献
94.
Comparative electrophoretic phenotypes of 18 of the 32 species of the lizard genus Varanus have been determined for four proteins. The animals studied were representative of species from Africa, Israel, Southeast Asia and Australia. Malate dehydrogenase (A2) exhibited a single phenotype throughout. Lactate dehydrogenase (B4) showed four distinctive electrophoretic forms which grouped the various subgenera as follows: (1) Polydaedalus, Empagusia (African); (2) Psammosaurus (Israel); (3) three species of Varanus, V. gouldii, V. spenceri, V. mertensi (Australian); (4) Dendrovaranus, Indovaranus (Southeast Asian), other Varanus species, Odatria (Australian). Electrophoretic and previously reported karyotypic data were used to interpret the phylogenetic relationships as well as the mode and direction of evolution of these animals. In particular, the results questioned the reality of the subgenus Varanus as a taxonomic unit, since four distinct karyotypic forms and two LDH-B4 phenotypes were observed for these animals, of which one belongs to another subgenus. Serum albumin and carbonic anhydrase phenotypes were of little use in deciding phenotypic groupings. 相似文献
95.
BRIAN P. EYDEN 《The Journal of eukaryotic microbiology》1975,22(3):336-344
SYNOPSIS. Light and electron microscope observations on Dunaliella primolecta Butcher from logarithmic and stationary phases of batch cultures are correlated. Except for the lack of a cell wall the fine structure has typical volvocid features. The transition from logarithmic to stationary phase is marked by changes in content and size of cytoplasmic vacuoles, accumulation of cytoplasmic lipid, accumulation of starch in the plastid matrix, and by the formation of autophagosome-like bodies. The organelles in stationary-phase flagellates are closely packed together because of the cytoplasmic lipid and starch-distended chloroplast. Organisms from logarithmic phase have an abundant ribosome-packed groundplasm supporting the organelles. In the cytochemical demonstration of acid phosphatase activity, Golgi cisternae and smooth and coated Golgi vesicles contain Gomori reaction product. The possible roles of the Golgi apparatus in this flagellate are discussed. 相似文献
96.
The new clerodane diterpene 1 was isolated from the aerial parts of Baccharis tucumanensis (Compositae). Its structure has been determined from spectral data combined with chemical transformations. 相似文献
97.
《Cell》2022,185(23):4409-4427.e18
98.
TROY G. MURPHY TIFFANY T. PHAM 《Biological journal of the Linnean Society. Linnean Society of London》2012,106(3):673-681
Coloration plays an important role in sexual and social communication, and in many avian species both males and females maintain elaborate colours. Recent research has provided strong support for the hypothesis that elaborate female traits can be maintained by sexual or social selection; however, most research on female ornamentation has focused on pigment‐based colours, and less is known about how structural colours are maintained. Both sexes of the turquoise‐browed motmot (Eumomota superciliosa) have a blue‐green racket‐tipped tail, and it remains unknown if tail coloration serves as a sexual or social signal in one or both sexes. Here, we describe sexual dichromatism in the blue‐green portion of the tail racket, and we test for a relationship between coloration and condition, as indicated by growth bars. Tail colour of both sexes has a similar spectral shape, and there is significant, although moderate, sexual dichromatism: males are brighter than females, and males have marginally greater blue‐green saturation than females. The length of feather grown per day is positively related to overall feather brightness, but this relationship is only present in males. The relationship between male coloration and condition suggests that tail colour has the potential to convey information about individual quality during mate choice or contest competition. The lack of a similar relationship in females suggests that female tail colour does not convey the same condition‐dependent information that we suggest may be reflected by male colour. Female tail colour may therefore reflect other aspects of condition, be involved in other (non‐condition‐dependent) forms of communication, or be expressed as a non‐functional byproduct of genetic correlation between the sexes. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 106 , 673–681. 相似文献
99.
Núria Cerdà‐Costa Francesc Xavier Gomis‐Rüth 《Protein science : a publication of the Protein Society》2014,23(2):123-144
The cleavage of peptide bonds by metallopeptidases (MPs) is essential for life. These ubiquitous enzymes participate in all major physiological processes, and so their deregulation leads to diseases ranging from cancer and metastasis, inflammation, and microbial infection to neurological insults and cardiovascular disorders. MPs cleave their substrates without a covalent intermediate in a single‐step reaction involving a solvent molecule, a general base/acid, and a mono‐ or dinuclear catalytic metal site. Most monometallic MPs comprise a short metal‐binding motif (HEXXH), which includes two metal‐binding histidines and a general base/acid glutamate, and they are grouped into the zincin tribe of MPs. The latter divides mainly into the gluzincin and metzincin clans. Metzincins consist of globular ~130–270‐residue catalytic domains, which are usually preceded by N‐terminal pro‐segments, typically required for folding and latency maintenance. The catalytic domains are often followed by C‐terminal domains for substrate recognition and other protein–protein interactions, anchoring to membranes, oligomerization, and compartmentalization. Metzincin catalytic domains consist of a structurally conserved N‐terminal subdomain spanning a five‐stranded β‐sheet, a backing helix, and an active‐site helix. The latter contains most of the metal‐binding motif, which is here characteristically extended to HEXXHXXGXX(H,D). Downstream C‐terminal subdomains are generally shorter, differ more among metzincins, and mainly share a conserved loop—the Met‐turn—and a C‐terminal helix. The accumulated structural data from more than 300 deposited structures of the 12 currently characterized metzincin families reviewed here provide detailed knowledge of the molecular features of their catalytic domains, help in our understanding of their working mechanisms, and form the basis for the design of novel drugs. 相似文献
100.
A retrospective study of 25 FNAs (11 aspirates from primary tumours and 14 from recurrencies and metastases) from 15 synovial sarcomas was performed. The cytological findings were correlated with the histopathology and the value of immunohistochemical and electron microscopic examination as well as DNA‐ploidy and cytogenetic analysis for diagnosis were assessed. A reproducible cellular pattern with a reliable diagnosis of spindle cell sarcoma was possible provided that the aspirates were cell rich. However, a true biphasic pattern indicative of synovial sarcoma was only seen in one of the 25 specimens. Electron microscopic examination of the aspirates was a valuable adjunctive diagnostic method, whereas immunocytochemistry and DNA‐ploidy analysis were not. Immunohistochemical, electron microscopic and cytogenetic analysis were all valuable ancillary methods when performed on surgical specimens. Malignant haemangiopericytoma and fibrosarcoma were the most important differential diagnoses in the FNA specimens. 相似文献