Xanthophyll-cycle dynamics and rapid induction of anthocyanin synthesis in Eucalyptus nitens seedlings transferred to photoinhibitory conditions

The effects of rapid induction and subsequent relaxation of cold-induced photo-inhibition on pigment chemistry of Eucalyptus nitens (Deane and Maiden) Maiden seedling leaves was assessed. The seedlings were subjected to four treatments in a nursery, fertilised or non-fertilised and shaded or non-shaded, before induction of photoinhibition in a growth chamber. Within 2 days, growth chamber conditions decreased photochemical efficiency (Fv/Fm) and increased xanthophyll-cycle conversion ratio. This was associated with decreased levels of total chlorophyll and increased levels of xanthophyll-cycle pigments. After 8 days, Fv/Fm of all treatments rose. Anthocyanin Levels gradually increased until day eight, except in non-shaded, non-fertilised seedlings, which had high levels before the induction of photoinhibition. Visible spectroscopy indicated increased absorption between 500 and 590nm, indicative of anthocyanin absorption. Electron transport rate after xanthophyll-cycle relaxation was half that measured before induction of photoinhibition. The findings indicate that anthocyanins may provide a photoprotective role in E. nitens seedlings under conditions of photoinhibition.     

Development of a PCR method for the detection and quantification of benzoyl-CoA reductase genes and its application to monitored natural attenuation

Benzoyl coenzyme A reductase (BCR) catalyzes dearomatization of benzoyl coenzyme A (benzoyl-CoA), which is the central step in the anaerobic degradative pathways for a variety of aromatic compounds. This study developed a PCR method for the detection and quantification of BCR genes in bacterial strains and environmental samples. PCR primers were designed by aligning known BCR genes in Thauera, Azoarcus and Rhodopseudomonas species, and their utility was assessed by amplifying BCR fragments from aromatic-hydrocarbon degrading anaerobes and other bacteria. BCR fragments with the expected sizes were obtained from denitrifying and phototrophic aromatics degraders. The positive signals were also obtained from Geobacter metallireducens and xylene-degrading sulfate-reducing bacterium (strain mXyS1) but not from other aromatics-degrading sulfate-reducing bacteria and aerobic bacteria. When the PCR was used for analyzing a natural attenuation (NA) site, the positive signal was obtained only from gasoline-contaminated groundwater; sequence analysis of these amplicons revealed that most of them exhibited substantial similarities to the known BCRs. Quantitative competitive PCR analysis estimated BCR-gene copies to account for 10–40% of bacterial 16S rRNA gene copies in the contaminated groundwater, indicating that bacteria possessing BCR genes were highly enriched in the contaminated groundwater. In microcosm bioremediation tests using the contaminated groundwater, the copy number of BCR gene was approximately 10-fold increased in the course of aromatics degradation under denitrifying conditions but not under sulfidogenic conditions. These results suggest the utility of the PCR method for assessing the potential of denitrifying bacteria for aromatic-compound degradation in groundwater.     

Recombinant Sendai virus vectors deleted in both the matrix and the fusion genes: efficient gene transfer with preferable properties

BACKGROUND: Sendai virus (SeV) is a new type of cytoplasmic RNA vector, which infects and replicates in most mammalian cells, directs high-level expression of the genes on its genome and is free from genotoxicity. In order to improve this vector, both the matrix (M) and fusion (F) genes were deleted from its genome. METHODS: For the recovery of the M and F genes-deleted SeV (SeV/DeltaMDeltaF), the packaging cell line was established by using a Cre/loxP induction system. SeV/DeltaMDeltaF was characterized and compared with wild-type and F or M gene-deleted SeV vectors in terms of transduction ability, particle formation, transmissible property and cytotoxicity. RESULTS: SeV/DeltaMDeltaF was propagated in high titers from the packaging cell line. When this vector was administered into the lateral ventricle and the respiratory tissue, many of the ependymal and epithelial cells were transduced, respectively, as in the case of wild-type SeV. F gene-deletion made the SeV vector non-transmissible, and M gene-deletion worked well to inhibit formation of the particles from infected cells. Simultaneous deletions of these two genes in the same genome resulted in combining both advantages. That is, both virus maturation into particles and transmissible property were almost completely abolished in cells infected with SeV/DeltaMDeltaF. Further, the cytopathic effect of SeV/DeltaMDeltaF was significantly attenuated rather than that of wild type in vitro and in vivo. CONCLUSIONS: SeV/DeltaMDeltaF is an advanced type of cytoplasmic RNA vector, which retains efficient gene transfer, gains non-transmissible properties and loses particle formation with less cytopathic effect.     

上海城市绿地植物群落降噪功能及其影响因子

利用因子分析法,对上海19类绿地植物群落30m宽度的噪声相对衰减量与8个群落结构因子的关系进行了分析.结果表明:绿地群落的降噪效果明显,其与草坪的降噪效果存在极显著差异(P<0.01),声波在绿地中有额外的声衰减.3组不同降噪能力绿地群落的30m噪声相对衰减量分别为≥10dB(A)、6～10dB(A)和?6dB(A);影响绿地群落降噪效果的结构因子为叶面积指数、群落平均枝下高、平均高度、盖度和平均冠幅,它们与噪声相对衰减量的相关系数分别为0.343、0.318、0.285、0.226和0.193.结构因子的累积贡献率达65.47%.在降噪绿地设计中应着重考虑这5个结构因子.     

The transmission of advertisement calls in Central American frogs

Acoustic communication signals change over distance due to lossof amplitude and fidelity, and it is assumed that signal degradationinfluences the receiver's ability to detect and decode signals.The degree of degradation depends on the signal's structureand the environment through which it transmits. We broadcastthe advertisement calls of 22 species of Central American frogsat two heights within forested and open environments in Panama. Werecorded these calls at five distances from the source and estimatedsignal degradation with a cross-correlation analysis, a measurethat combines the effects of decrement in signal amplitude andfidelity. Calls degraded less when broadcast higher above theground compared to on the ground, and less in open habitat comparedto forested habitat; there was an additional interaction betweenheight and environment. Furthermore, calls with low dominant frequenciesexperienced less degradation than calls with high dominant frequencies.There was no evidence, however, that the calls of these frogs haveevolved to maximize habitat-specific transmission.     

Assessment of corneal viscoelasticity using elastic wave optical coherence elastography

The corneal viscoelasticity have great clinical significance, such as the early diagnosis of keratoconus. In this work, an analysis method which utilized the elastic wave velocity, frequency and energy attenuation to assess the corneal viscoelasticity is presented. Using phase‐resolved optical coherence tomography, the spatial‐temporal displacement map is derived. The phase velocity dispersion curve and center frequency are obtained by transforming the displacement map into the wavenumber‐frequency domain through the 2D fast Fourier transform (FFT). The shear modulus is calculated through Rayleigh wave equation using the phase velocity in the high frequency. The normalized energy distribution is plotted by transforming the displacement map into the spatial‐frequency domain through the 1D FFT. The energy attenuation coefficient is derived by exponential fitting to calculate the viscous modulus. Different concentrations of tissue‐mimicking phantoms and porcine corneas are imaged to validate this method, which demonstrates that the method has the capability to assess the corneal viscoelasticity.     

A framework for studying transient dynamics of population projection matrix models

Empirical models are central to effective conservation and population management, and should be predictive of real-world dynamics. Available modelling methods are diverse, but analysis usually focuses on long-term dynamics that are unable to describe the complicated short-term time series that can arise even from simple models following ecological disturbances or perturbations. Recent interest in such transient dynamics has led to diverse methodologies for their quantification in density-independent, time-invariant population projection matrix (PPM) models, but the fragmented nature of this literature has stifled the widespread analysis of transients. We review the literature on transient analyses of linear PPM models and synthesise a coherent framework. We promote the use of standardised indices, and categorise indices according to their focus on either convergence times or transient population density, and on either transient bounds or case-specific transient dynamics. We use a large database of empirical PPM models to explore relationships between indices of transient dynamics. This analysis promotes the use of population inertia as a simple, versatile and informative predictor of transient population density, but criticises the utility of established indices of convergence times. Our findings should guide further development of analyses of transient population dynamics using PPMs or other empirical modelling techniques.     

Effects of hydraulic architecture and spatial variation in light on mean stomatal conductance of tree branches and crowns

In a Pinus taeda L. (loblolly pine) plantation, we investigated whether the response to vapour pressure deficit (D) of canopy average stomatal conductance (G(S)) calculated from sap flux measured in upper and lower branches and main stems follows a hydraulically modelled response based on homeostasis of minimum leaf water potential (Psi(L)). We tested our approach over a twofold range of leaf area index (L; 2-4 m(2) m(-2)) created by irrigation, fertilization, and a combination of irrigation and fertilization relative to untreated control. We found that G(S) scaled well from leaf-level porometery [porometry-based stomatal conductance (g(s))] to branch-estimated and main stem-estimated G(S). The scaling from branch- to main stem-estimated G(S) required using a 45 min moving average window to extract the diurnal signal from the large high-frequency variation, and utilized a light attenuation model to weigh the contribution of upper and lower branch-estimated G(S). Our analysis further indicated that, regardless of L, lower branch-estimated G(S) represented most of the main stem-estimated G(S) in this stand. We quantified the variability in both upper and lower branch-estimated G(S) by calculating the SD of the residuals from a moving average smoothed diurnal. A light model, which incorporated penumbral effects on vertical distribution of direct light, was employed to estimate the variability in light intensity at each canopy level in order to explain the increasing SD of both upper and lower branch-estimated G(S) with light. The results from the light model showed that the upper limit of the variability in individual branch-estimated G(S) could be attributed to incoming light, but not the variation below that upper limit. A porous medium model of water flow in trees produced a pattern of variation below the upper limit that was consistent with the observed variability in branch-estimated G(S). Our results indicated that stems acted to buffer leaf- and branch-level variation and might transmit a less-variable water potential signal to the roots.