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81.
John P. Carulli De-Mao Chen William S. Stark Daniel L. Hartl 《Journal of molecular evolution》1994,38(3):250-262
Phylogenetic and physiological methods were used to study the evolution of the opsin gene family in Drosophila. A phylogeny based on DNA sequences from 13 opsin genes including representatives from the two major subgenera of Drosophila shows six major, well-supported clades: The blue opsin clade includes all of the Rhl and Rh2 genes and is separated into two distinct subclades of Rhl sequences and Rh2 sequences; the ultraviolet opsin clade includes all Rh3 and Rh4 genes and bifurcates into separate Rh3 and Rh4 clades. The duplications that generated this gene family most likely took place before the evolution of the subgenera Drosophila and Sophophora and their component species groups. Numerous changes have occurred in these genes since the duplications, including the loss and/or gain of introns in the different genes and even within the Rhl and Rh4 clades. Despite these changes, the spectral sensitivity of each of the opsins has remained remarkably fixed in a sample of four species representing two species groups in each of the two subgenera. All of the strains that were investigated had R1-6 (Rhl) spectral sensitivity curves that peaked at or near 480 nm, R7 (Rh3 and Rh4) peaks in the ultraviolet range, and ocellar (Rh2) peaks near 420 nm. Each of the four gene clades on the phylogeny exhibits very conservative patterns of amino acid replacement in domains of the protein thought to influence spectral sen sitivity, reflecting strong constraints on the spectrum of light visible to Drosophila. 相似文献
82.
After considering the need for quality control in NAA, the concept of quality in NAA procedures themselves is discussed, and
some important factors identified. Two approaches to improve quality are then described in more detail. The first concerns
the unique ability of NAA using different isotopic reactions and different modes (INAA/RNAA) to provide independent data sets
in the same laboratory, thus allowing internal validation or crosschecking. The second discusses the need for chemical yield
measurements in RNAA and the advantages of the radioisotopic tracer technique. Some recent advances and further possibilities
for this use of tracers are listed. 相似文献
83.
Georgina L. Dasilva 《International journal of primatology》1994,15(5):655-680
A group of Colobus polykomosat Tiwai, Sierra Leone, demonstrated seasonal flexibility in its diet, with seeds, young leaves, and mature leaves each dominating
the diet at different times. Comparison of food consumption with phenological data indicates that seeds are eaten whenever
available and are preferred to other foods, while young leaves are preferred to mature leaves. Colobus polykomosalso prefer liane to tree leaves, despite the relatively high quality of mature tree foliage at the Tiwai site. Analysis of
protein, fiber, and energy values of foods selected and items available, but not eaten, suggests that preference is related
to protein and energy maximization. Leguminous plants, especially Papilionaceae and Mimosaceae, are highlighted as important
food sources for C. polykomos;seeds and leaves from these families have a high nitrogen content, and the protein content of leguminous seeds often equals
or exceeds that found in leaves. It is predicted, therefore, that colobines living in habitats with a high density of legumes
will feed heavily on seeds, subject to constraints such as seasonal availability. Where suitable leguminous species are less
common, a mixture of fruits, seeds, and young or mature leaves or both is likely to be selected. The results of this and other
recent studies of colobines do not support the notion that colobines are specialist folivores. 相似文献
84.
The induction of parthenocarpic fruit set was investigated using the apple cvs. Golden Delicious and Jonagold. The gibberellins GA3, GA4, GA5 and GA7 and the synthetic phenylurea-type cytokinin CPPU (N-(2-chloro-4-pyridyl)-N-phenylurea), were applied alone and in combination to unpollinated flowers at the end of petal fall. Gibberellins induced only a marginal final set of parthenocarpic fruits. CPPU sprays were more effective, particularly in the first year. When applied in combination, CPPU and gibberellins had a positive synergistic effect on parthenocarpic fruit set and fruit size, but a negative effect on flower induction the next year. After CPPU + GA sprays, percent fruit set was similar, or greater, compared to natural pollinated trees. The parthenocarpic fruits induced by CPPU + GA had an increased length to diameter ratio. CPPU stimulated, and GA4 and GA7 reduced, the russeting of the parthenocarpic fruits. The internal quality of the fruits was hardly affected, but Ca-deficiency symptoms occurred more frequently in parthenocarpic fruits. 相似文献
85.
Gary C. du Moulin Zorina Pitkin Yuan-Jin Shen Evelyn Conti Jean Ko Stewart Carla Charles Dylan Hamilton 《Cytotechnology》1994,15(1-3):365-372
Somatic cell and gene therapy involve the application of biological technologies to an individual patient through the use of living cells which provide a therapeutic benefit (Aliski, 1991). Various forms of cellular and gene therapies are being developed and evaluated in an increasing number of clinical trials for congential and acquired disorders. The potential and progress of these therapeutic applications have resulted in an increasing effort by the Food and Drug Administration (FDA) to develop the regulatory framework under which these therapeutic approaches would insure safety and efficacy, the primary mandate of the FDA.Over five years ago Cellcor began to define the parameters, specifications, and conditions relevant to a Quality Assurance/Quality Control (QA/QC) program that has evolved to insure safety and maximize the efficacy of applications of the company'sex vivo technology, autolymphocyte therapy. Autolymphocyte therapy is an outpatient form of somatic cell immunotherapy based upon the infusion of T cells that have been activatedex vivo using a combination of previously generated autologous cytokines and an anti-CD3 monoclonal antibody.We have been able to demonstrate the feasibility for the safe, controlled, and consistent preparation and delivery of a cellular therapy by application of relevant GMP regulations. This presentation reviews aspects of this program and chronicles our experience which at present amounts to over 4400 infusions for over 700 patients. This program provides a high degree of assurance that a cellular therapy program can be carried out in a multisite mode involving hundreds of patients through the strict adherence to cGMP as set forth in existing regulations. It would be prudent that developers of cellular andex vivo gene therapies establish a similar cell processing and QA/QC infrastructure at an early developmental stage to optimize safety and reproducibility and facilitate regulatory review. 相似文献
86.
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88.
长白山站的研究数据库管理系统翟永华,赵士洞(中国科学院沈阳应用生态研究所110015)Date-BaseManagementSysteminChangbaishanForestEcosystemResearchStation.¥ZhaiYonghua... 相似文献
89.
The sex ratio in final-instar larvae of a birch-feeding, free-living solitary sawfly, Dineura virididorsata, was investigated in Finnish Lapland. The prepupal proportion of females, pooled over ten sites, was 56%, and at four individual
sites the sex ratio was significantly female-biased. Larval survival from egg to prepupae did not differ between the sexes.
This suggests a femalebiased primary sex ratio in the field. The sex ratio varied among the sites but not among host trees
within sites. Contrary to previous results with hymenopterans, we did not find that differences in the sex ratio depended
on forage quality: site-specific or tree-specific sex ratios did not correlate with the average prepupal weight. A literature
search indicated that female-biased sex ratios are also common in other free-living sawflies. We are unable to explain sex
ratios of Dineura virididorsata or other free-living sawflies with existing general models. 相似文献
90.
An important component of the interaction between macroinvertebrates and leaf litter in streams in the extent to which consumers
can differentiate between undecomposed and decomposing leaves. The detritivores Gammarus pulex and Asellus aquaticus fed preferentially on conditioned rather on unconditioned leaf material. Growth in A. aquaticus was significantly reduced when unconditioned leaves were provided, but in G. pulex no significant effect of conditioning on growth was observed. The capacity of G. pulex to tolerate reductions in food quality seems to be a consequence of a compensatory system in which respiration rates change
to compensate for reductions in food quality. In this way a constant growth rate is maintained. Increases in ingestion rates
to compensate for low quality food were not observed. 相似文献