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排序方式: 共有536条查询结果,搜索用时 15 毫秒
51.
The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by the Human Proteome Organisation (HUPO) as a Kidney Disease Initiative under an umbrella of the HUPO Disease Biomarker Initiative (DBI), and more recently was approved as the HKUPP Initiative in 2007. Several sub-projects have been planned to achieve the ultimate goals. The most pressing is the establishment of "standard protocols and guidelines for urine proteome analysis". This sub-project had been extensively discussed during the first HKUPP symposium (during 6(th) HUPO Annual World Congress--October 2007, Seoul, Korea) and second workshop (during 40(th) American Society of Nephrology Renal Week--November 2007, San Francisco, CA, USA). Additional data and references have been collected after the symposium and workshop. An initial draft of standard protocols and guidelines for proteome analysis of non-proteinuric urine (urine protein excretion < or =150 mg/day) will soon be released as the first HKUPP product. 相似文献
52.
Suzuki M Endo N Nakano Y Kato H Kishiro T Asahina K 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2008,178(2):149-156
This study examined the distribution pattern of aquaporin-2 (AQP2), relative medullary thickness (RMT) and urine properties
in the bottlenose dolphin Tursiops
truncatus and Baird’s beaked whale Berardius bairdii. Immunohistochemical studies revealed that AQP2 was localized in the collecting tubules/ducts of both species’ renicules,
as in terrestrial mammals. The collecting ducts with AQP2 were thinner and arranged more densely in the dolphin than in the
whale. RMT values in the renicule were moderate in both species, but were significantly higher in the dolphin (6.0 ± 0.9)
than the whale (4.9 ± 0.7). Urine of the bottlenose dolphin is comparatively concentrated (osmolality: 1715.7 ± 279.4 mOsm kg−1, Na+: 490.1 ± 87.9 mmol l−1, Cl−: 402.7 ± 79.6 mmol l−1, K+: 80.7 ± 25.8 mmol l−1, urea nitrogen: 703.5 ± 253.9 mmol l−1), while urine of the dead Baird’s beaked whale is less concentrated (osmolality: 837.5 ± 293.8 mOsm kg−1, Na+: 192.9 ± 81.5 mmol l−1, Cl−: 159.9 ± 71.4 mmol l−1, K+: 44.3 ± 29.5 mmol l−1, urea nitrogen: 270.7 ± 120.3 mmol l−1). These data suggest it is possible that the differences in these renal morphological features may be related in some way
to the difference in urine composition between the species, although further studies are necessary.
M. Suzuki and N. Endo are equal contributors to this study. 相似文献
53.
This paper describes a colorimetric sensing approach for the determination of adenosine triphosphate (ATP) using aptamer-modified gold nanoparticles (Apt-Au NPs). In the absence of the analytes, the color of the Apt-Au NPs solution changed from wine-red to purple as a result of salt-induced aggregation. Binding of the analytes to the Apt-Au NPs induced folding of the aptamers on the Au NP surfaces into four-stranded tetraplex structures (G-quartet) and/or an increase in charge density. As a result, the Apt-Au NPs solution was wine-red in color in the presence of the analytes under high salt conditions. For mixtures of ATP (20.0–100.0 nM), Apt-Au NPs (3.0 nM), 10.0% poly(ethylene glycol), 0.2 μM TOTO-3, 150.0 mM NaCl, 15.0 mM KCl, and 16.0 mM Tris–HCl (pH 7.4), a linear correlation (R2 = 0.99) existed between the ratio of the extinctions of the Apt-Au NPs at 650 and 520 nm (Ex650/520) and the concentration of ATP. The limit of detection for ATP was 10.0 nM. The practicality of this simple, sensitive, specific, and cost-effective approach was demonstrated through the determination of the concentration of adenosine in urine samples. 相似文献
54.
Wang Y Chen Y Zhang Y Wu S Ma S Hu S Zhang L Shao C Li M Gao Y 《Biochemical and biophysical research communications》2008,371(3):385-390
Glomerular diseases are leading causes of end-stage renal diseases worldwide. They are considered to be consequences of injury primarily to the three types of glomerular cells. Differential diagnosis typically relies on invasive biopsy findings. We expected that injuries of different glomerular cells would cause different changes in urinary proteome. The goal of this study was to identify differential urinary proteins distinguishing between injuries of different glomerular cells before significant histopathologic changes. Adriamycin nephropathy and Thy1.1 glomerulonephritis were employed as models with different primary impaired cells. ConA-enriched urinary glycoproteome on day3 were profiled by gel-free shotgun tandem mass spectrometry, and compared with self-healthy controls to identify differential urinary proteins for each model. By comparing the changes of the differential proteins between these two models, we identified 39 proteins with different directions of changes, which may potentially be useful in differentiation; and 7 proteins with the same direction of changes, which may be potential indicators of early renal damage. These differential proteins were of several origins: plasma proteins, proteins with urine or kidney specificity, proteins without tissue-specificity (mainly inflammatory mediators) etc. Our results may help better understand the effects of injuries of different glomerular cells at the initial stage, and lead to the discovery of novel early diagnostic markers for human focal segmental glomerulosclerosis (FSGS) and mesangioproliferative glomerulonephritis (MsPGN) which have the same primary impaired cells with adriamycin nephropathy and Thy1.1 glomerulonephritis, respectively. 相似文献
55.
笼养雌性果子狸同性数量对发情状态的影响及繁殖季节尿液雌激素浓度的变化 总被引:1,自引:0,他引:1
本研究试图揭示 (1)社会环境影响雌性果子狸 (Pagumalarvata)的交配日期 ,(2 )哺乳期母兽有雌二醇浓度升高的现象。通过检查是否排出交配栓并结合交配行为的发生与否确定开始交配的日期 ,使用放射免疫方法测定尿中雌二醇浓度。结果表明 (1)一雄两雌组中优势雌兽开始交配的日期显著早于劣势雌兽和单雌组雌兽 ,劣势雌兽和单雌组雌兽之间无差异 ;交配期优势雌兽尿液中雌二醇浓度明显高于劣势雌兽 ,而与单雌组中的雌兽无差异 ,但单雌组中雌兽尿液中雌二醇的浓度明显高于劣势雌兽雌二醇的浓度 ;(2 ) 5头母兽产后第二天雌二醇浓度开始上升 ,在第 5天时达到高峰 ,其中 4头母兽的雌二醇浓度共出现了两个峰值 ,其间隔为 16 75± 4 4 6 (4)天。上述结果说明 :(1)社会影响仅对优势雌性个体的繁殖有促进作用 ;(2 )哺乳期雌二醇水平的升高是季节性多次发情的基础。 相似文献
56.
Vronique Andre Pierre Lebailly Edwige Deslandes Michel Henry-Amar Pascal Gauduchon 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2002,520(1-2):199-205
Comparative extraction efficiency of the pre-packed Bakerbond®-spe-SDB-1 resin and of Amberlite®-AD2 (XAD-2) resin, for the preparation of urine extracts in biomonitoring studies. Urine extracts were prepared in parallel with the Bakerbond® column and with the classical XAD-2 resin from urines (1) spiked with mutagenic chemicals, (2) collected from patients after chemotherapy, and (3) from smokers. Mutagenic activities were evaluated on Salmonella typhimurium tester strains TA97a, TA98, TA100 and TA102 with and without S9 mix. Mutagenic activities obtained with Bakerbond® extracts were almost always higher or at least equivalent to those prepared on XAD-2 resin. Similar results were observed for the three urine sample groups. When fully validated, the use of the pre-packed columns will be more convenient and time-saving for large population studies. 相似文献
57.
J. Mühling M. G. Dehne M. Fuchs A. Sablotzki S. Weiss J. Spatz G. Hempelmann 《Amino acids》2001,21(3):303-318
Summary. Currently we know not more than 50 patients who show an interesting combination of increased plasma ornithine concentrations,
postprandial hyperammonemia, and homocitrullinuria (HHH-syndrome). Since exact knowledge of this severe, although rare syndrome
is important for any perioperative or intensive medical treatment concerning therapy and progression of the disease, we report
a comprehensive study on a 32-year old woman with this rare multifaceted disorder who had to undergo general anaesthesia.
For the first time amino acid status in plasma, urine, cerebrospinal fluid and especially polymorphonuclear leucocytes, which
in the investigation showed to be valuable tool for evaluating amino acid metabolism in nucleated cells in HHH-syndrome, and
further important pathophysiologic indicators of cellular and metabolic function have been conscientiously investigated and
compared. The pathophysiological repercussions of our results as well as the recommendations for conscientious therapeutical
management are discussed.
Received September 9, 1999 Accepted July 1, 2000 相似文献
58.
An analytical method using double focusing sector field inductively — coupled plasma mass spectrometry (sector field ICPMS) for rapid simultaneous determination of 42 elements in urine is described. Sample preparation consisted of 20-fold dilution with 0.14 mol/l nitric acid in ultrapure water. The importance of controlling possible contamination sources at different sample preparation and analysis stages in order to achieve adequate method detection limits (DL) is emphasized. Correction for matrix effects was made using indium and lutetium as internal standards. Different approaches for accuracy assessment in urine analysis are evaluated. Additional information on trace element concentrations in a urine reference material is given. Between-batch precision was assessed from the analysis of separately prepared aliquots of the reference material and was better than 10% RSD for 32 of the elements. The robustness of the procedure was tested by analysis of about 250 samples in one analytical run lasting more than 50 hours. A statistical summary of results for 19 urine samples from non-exposed subjects is presented. For a majority of the elements tested concentrations were higher than the detection limit of the method. 相似文献
59.
J. R. Poortmans E. Blommaert M. Baptista M. E. De Broe E. J. Nouwen 《European journal of applied physiology and occupational physiology》1997,76(1):88-91
Post-exercise proteinuria is a common phenomenon in healthy subjects. Previous studies have used albumin (Alb) and β2-microglobulin (β2-m) molecules as representatives of high- and low-molecular-weight proteins. Recently, more specific markers of the human
kidney proximal tubule have been used to identify the precise site of alterations. Active male subjects underwent two strenuous
runs, one 400-m run and one 3000-m run. Urine was collected from the subjects before and after each event. Total protein (TP),
Alb, α1-microglobulin (α1-m), β2-m, intestinal alkaline phosphatase (IAP), tissue-nonspecific alkaline phosphatase (TNAP) and N-acetyl-β-d-glucosaminidase (NAG) were determined for each sample. The short-distance run (400 m) resulted in the largest increases (P ≤ 0.05) in TP (31-fold), Alb (100-fold) and β2-m (164-fold) as compared to the long-distance run (3000-m). The α1-m excretion rates were increased to a lesser extent by the exercises. The IAP activity was slightly increased (+90%) by the
400-m run while the TNAP and NAG activities showed a 6.8-fold and a 3.6-fold increase, respectively, after this event. Smaller
increases were recorded for the long-distance run (P = 0.05). To conclude, the present investigation showed that: (1) post-exercise proteinuria is related to the absolute intensity
of exercise; (2) the impairment of protein reabsorption is revealed better by changes in Alb and β2-m; (3) changes in TNAP and NAG activities could reveal biochemical modifications that occur in the proximal tubule, particularly
at the S1-S2 segment.
Accepted: 31 January 1997 相似文献
60.
AimThis study aims to determine the changes induced by a maximal exercise test until exhaustion on the serum and urinary concentrations of Magnesium (Mg), Phosphorous (P), Rubidium (Rb) and Strontium (Sr) in athletes (AG) and sedentary students (SG).MethodsFifty subjects participated in the study divided into two groups. In AG there were twenty-five male athletes and in SG there were twenty-five male sedentary students. Both groups performed an exercise test until exhaustion, starting at 8 or 10 km/h respectively, and increasing the speed at 1 km/h every 400 m. Serum and urine samples were obtained from all participants before and after the test.ResultsRegarding the basal status, AG showed lower values of Mg in serum (p < 0.05) and urine (p < 0.01), but higher concentrations of serum P (p < 0.05) in comparison to SG. Comparing the pre and post-test values, corrected or non-corrected for hemoconcentration in serum and for creatinine in urine, AG showed a decrease in serum Mg (p < 0.05), in serum P (p < 0.01) and in urinary Sr (p < 0.01) while an increase was observed in urinary P (p < 0.05) and in urinary Rb (p < 0.05).ConclusionsIt can be concluded that a treadmill test until exhaustion leads to changes in serum and urinary concentrations of minerals in both AG and SG males. This may reflect an adaptive response of the body to overcome the physical stress and, in some cases, to avoid loss of these elements. 相似文献