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排序方式: 共有653条查询结果,搜索用时 78 毫秒
651.
Improvement of mammalian cell culture performance through surfactant enabled concentrated feed media
Patrick Hossler Sean McDermott Christopher Racicot John C. H. Fann 《Biotechnology progress》2013,29(4):1023-1033
The design of basal and feed media in mammalian cell culture is paramount towards ensuring acceptable upstream process performance in various operation modes, especially fed‐batch culture. Mammalian cell culture media designs have evolved from the classical formulations designed by Eagle and Ham, to today's formulations designed from continuous improvement and statistical frameworks. Feed media is especially important for ensuring robust cell growth, productivity, and ensuring the product quality of recombinant therapeutics are within acceptable ranges. Numerous studies have highlighted the benefit of various media designs, supplements, and feed addition strategies towards the resulting cell culture process. In this work we highlight the use of a top‐down level approach towards feed media design enabled by the use of select surfactants for the targeted enrichment of a chemically defined feed media. The use of the enriched media was able to improve product titers at g/L levels, without adversely impacting the growth of multiple Chinese Hamster Ovary cell lines or the product quality of multiple recombinant antibodies. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1023–1033, 2013 相似文献
652.
Abstract. Data on distribution and abundance of rare plant species on isolated coastal dunes along the rocky coast of northwestern Spain were compared with the local floristic literature in order to reconstruct past distribution and local extinction of species. Species extinction was not confined to species with a narrow distribution but also included widely distributed ones. Moreover, there was no evidence of colonization. Species number was related to beach and dune area, but species differed in the minimum area required for occurrence. The largest dune reserve possible would probably be insufficient to avoid further extinctions, so also small areas containing particular species should be preserved. There was no significant relationship between abundance of species in the local dune communities and their total distribution, and there were few satellite species. The former might be attributed to low fertility and habitat diversity. 相似文献
653.
GAVIN BROOKS 《Pigment cell & melanoma research》1994,7(6):451-457
Since its discovery more than a decade ago [Wu et al., 1982; Rozengurt et al., 1983], the 80-87 kDa myristoylated a lanine-rich C-kinase substrate (80K/MARCKS) protein has attracted a great deal of attention from researchers interested in cell growth and tumour progression. However, despite its ubiquitous distribution, a definitive functional role for 80K/MARCKS has not been found. The purpose of this review is to describe the properties, distribution and regulation of 80K/MARCKS and to discuss some of the most recent findings, both from our laboratory and from others, that have suggested a functional role for this protein in modulating cell growth and tumour progression. Furthermore, I will present data from our laboratory that implicates 80K/MARCKS as a novel tumour suppressor in cells of melanocyte origin. 相似文献