全文获取类型
收费全文 | 1319篇 |
免费 | 73篇 |
国内免费 | 27篇 |
出版年
2024年 | 1篇 |
2023年 | 15篇 |
2022年 | 6篇 |
2021年 | 9篇 |
2020年 | 19篇 |
2019年 | 29篇 |
2018年 | 20篇 |
2017年 | 33篇 |
2016年 | 34篇 |
2015年 | 31篇 |
2014年 | 30篇 |
2013年 | 52篇 |
2012年 | 21篇 |
2011年 | 24篇 |
2010年 | 28篇 |
2009年 | 54篇 |
2008年 | 61篇 |
2007年 | 81篇 |
2006年 | 66篇 |
2005年 | 66篇 |
2004年 | 42篇 |
2003年 | 33篇 |
2002年 | 51篇 |
2001年 | 66篇 |
2000年 | 54篇 |
1999年 | 19篇 |
1998年 | 27篇 |
1997年 | 25篇 |
1996年 | 36篇 |
1995年 | 26篇 |
1994年 | 38篇 |
1993年 | 32篇 |
1992年 | 36篇 |
1991年 | 32篇 |
1990年 | 41篇 |
1989年 | 19篇 |
1988年 | 32篇 |
1987年 | 11篇 |
1986年 | 26篇 |
1985年 | 16篇 |
1984年 | 16篇 |
1983年 | 11篇 |
1982年 | 17篇 |
1981年 | 5篇 |
1980年 | 13篇 |
1978年 | 6篇 |
1977年 | 3篇 |
1976年 | 6篇 |
排序方式: 共有1419条查询结果,搜索用时 78 毫秒
131.
《Systematic and applied microbiology》2022,45(4):126336
Several pure cultures of alkaliphilic haloaloarchaea were enriched and isolated from hypersaline soda lakes in southwestern Siberia using amylopectin and fructans as substrates. Phylogenomic analysis placed the isolates into two distinct groups within the class Halobacteria. Four isolates forming group 1 were closely related to a recently described Natranaeroarchaeum sulfidigenes and the other three strains forming group 2 represent a novel genus-level phylogenetic lineage. All isolates are saccharolytic archaea growing with various starch-like alpha-glucans including soluble starch, amylopectin, dextrin, glycogen, pullulane and cyclodextrin. In addition, group 1 can use levan while group 2 – inulin (plant storage beta-fructans). Group 1 strains can also grow anaerobically with either glucose or maltose using elemental sulfur as the electron acceptor. Both groups are moderately alkaliphilic with a pH range for growth from 7.2 to 9.3 (optimum between 8.0–8.8) and low Mg-demanding extreme halophiles growing optimally at 4 M total Na+. The major respiratory menaquinone is MK-8:8 and the core biphytanyl lipids are dominated by archaeol (C20-C20) and a less abundant extended archaeol (C20-C25) with PG and PGP-Me as polar groups. The four isolates of group 1 are suggested to be classified into a new species as Natranaeroarchaeum aerophilus sp. nov. (type strain AArc-St1-1T = JCM 32519T). The three isolates of group 2 are proposed to form a new genus and species for which the name Natronocalculus amylovorans gen. nov., sp. nov. is suggested (type strain AArc-St2T = JCM 32475T). 相似文献
132.
Constitutive presence of antibiotic resistance genes within the bacterial community of a large subalpine lake 下载免费PDF全文
Andrea Di Cesare Ester M. Eckert Alessia Teruggi Diego Fontaneto Roberto Bertoni Cristiana Callieri Gianluca Corno 《Molecular ecology》2015,24(15):3888-3900
The fate of antibiotic resistance genes (ARGs) in environmental microbial communities is of primary concern as prodromal of a potential transfer to pathogenic bacteria. Although of diverse origin, the persistence of ARGs in aquatic environments is highly influenced by anthropic activities, allowing potential control actions in well‐studied environments. However, knowledge of abundance and space–time distribution of ARGs in ecosystems is still scarce. Using quantitative real‐time PCR, we investigated the presence and the abundance of twelve ARGs (against tetracyclines, β‐lactams, aminoglycosides, quinolones and sulphonamides) at different sampling sites, depths and seasons, in Lake Maggiore, a large subalpine lake, and in the area of its watershed. We then evaluated the correlation between each ARG and a number of ecological parameters in the water column in the deepest part of the lake. Our results suggest the constitutive presence of at least four ARGs within the bacterial community with a high proportion of bacteria potentially resistant to tetracyclines and sulphonamides. The presence of these ARGs was independent of the total bacterial density and temperature. The dynamics of tet(A) and sulII genes were, however, positively correlated with dissolved oxygen and negatively to chlorophyll a, suggesting that the resistant microbes inhabit specific niches. These observations indicate that the lake is a reservoir of antibiotic resistances, highlighting the need of a deeper understanding of the sources of ARGs and the factors allowing their persistence in waters. 相似文献
133.
Behavioural strategy of large perch Perca fluviatilis varies between a mesotrophic and a hypereutrophic lake 下载免费PDF全文
Behaviour of large perch Perca fluviatilis was studied in two lakes differing in environmental state i.e. mesotrophic v. hypereutrophic. A total of 20 adult perch P. fluviatilis (29–42 cm total length) in each lake were tagged with radio‐transmitters, tracked and located eight times a day during six 24 h tracking periods over a year, enabling detection of differences in diel activity patterns and habitat use during summer and winter under two different environmental regimes. During summer, P. fluviatilis in the mesotrophic lake showed a distinct crepuscular activity pattern and a change from pelagic residency during daytime towards the littoral zone at night. In contrast, P. fluviatilis in the hypereutrophic lake were active during the entire diel cycle and were spread throughout the lake also during dark. During winter, crepuscular patterns of activity were seen in both lakes. Condition factor of large P. fluviatilis did not differ between the two lakes. Thus, it is suggested that P. fluviatilis in the hypereutrophic turbid lake adopted an alternative behaviour for successful foraging, being uniformly active throughout the diel cycle. 相似文献
134.
通过对桑根达莱淖尔卤虫的养殖实验与卵囊解剖,研究了内蒙古沙漠小型盐湖投饵、施肥与自然状态3种营养模式下卤虫的生境、种群动态、生殖特征,分析了环境对卤虫资源的负载力。结果表明:1在起始种群相同的情况下,不同营养模式对种群结构与密度有显著影响;2不同营养模式对个体生长速度影响存在差异,投饵对加快个体生长速度效果最明显,但在性成熟速度方面不同营养模式没有出现统计学显著差异;3不同营养模式对卤虫的怀卵量、卵生/卵胎生比例有显著影响;与空白组相比,投饵组平均怀卵量提高了35.52%—72.71%,施肥组提高了11.34%—26.15%;4卤虫资源的环境负载力为0.3—0.4 kg/m3,加以补充肥料,可提高到0.45 kg/m3,在投喂饲料的情况下可以达到0.5 kg/m3;5卤虫蛋白可开发量按环境负载力的1/3估计,对照组、施肥组和投饵组的相应年开发量分别为2.61—2.98 kg/m3、4.5—5.4 kg/m3和7.51—8.67 kg/m3,滞育卵产量分别为0.73、1.10 g/m3和1.17 g/m3。 相似文献
135.
136.
Two new species of the genus Stenandra Lameere, 1912 are described: Stenandra saitoae from Sulawesi of Indonesia and Stenandra asiatica from Vietnam. A key to the species of the Stenandra is given. 相似文献
137.
Common reed die-back is a widely investigated phenomenon in Central Europe, not frequently recorded in S-European areas and almost unknown in the Mediterranean Basin. Symptoms of reed decline recently observed in the Italian Peninsula provided the starting point for a detailed investigation on a reed population in one of the largest freshwater ecosystems in Central Italy. The analyses were conducted over two vegetative seasons in 19 plots at seven locations. A set of 13 morphologic and phenologic reed traits were screened, monitored and statistically analysed. The data indicated the presence of the reed die-back syndrome in a wet Mediterranean ecosystem and enabled us to highlight a set of usable traits to detect the condition of decline. Among them, the stem height and diameter, the number of nodes, the relative growth rate and the lateral root diameter resulted the most significant factors highlighting the declining condition. Some environmental characteristics of the reed stands were also taken into account. The period of submersion and the presence of standing litter emerged as important features of the stands, strictly related to the degree of decline in the population. The results draw attention to the risk, in southern Europe too, of the loss of an ecosystem which plays an important role in biodiversity conservation. 相似文献
138.
The role of resource subsidies across ecosystem boundaries has emerged as an important concept in contemporary ecology. For lake ecosystems, this has led to interest in quantifying the contribution of terrestrial allochthonous carbon to aquatic secondary production. An inverse relationship between habitat area and the role of allochthonous subsidies has been documented on marine islands and assumed for lakes, yet there have been no tests of this pattern among benthic (lake bottom) consumers. Here, we used carbon stable isotopes to trace terrestrial allochthonous and benthic autochthonous carbon use by the crayfish Pacifastacus leniusculus over a gradient of lake area, productivity and urbanization. Consistent with findings from terrestrial islands, habitat size dictated the importance of allochthonous subsidies, as P. leniusculus transitioned from using predominantly terrestrial carbon in small lakes to an increased reliance on autochthonous production in larger lakes. However, shoreline urbanization interacted with this pattern, particularly for small lakes where greater urbanization resulted in reduced use of allochthonous resources. As such, we provide, to our knowledge, the first confirmation of the predicted relationship between habitat size and importance of allochthonous subsidies to lake benthic consumers, but found that urbanization can interfere with this pattern. 相似文献
139.
Recent research on relationships between diatoms and pH suggests that the genus Cyclotella exhibits a strong relationship with lake acidity, being almost totally absent below pH 5.5. This decline has been used as an indicator of lake acidification in paleolimnological studies. In this study C. stelligera V.H. and C. kützingiana Thwaites were abundant in Precambrian Shield lakes with pH as low as 4.5. Cyclotella comta (Ehr.) Kütz. was found in lakes of pH < 5.5, but maximum abundance was observed in lakes of pH > 5.5. Cyclotella michiganiana Skv. was found in lakes of pH < 6.0. These results indicate that the use of C. stelligera, C. kützingiana, and possibly C. comta, in paleolimnological investigations of lake acidification, should be approached with caution. These taxa may exhibit a decline in abundance with decreasing lakewater pH, but this is partially a morphometric effect not necessarily related to anthropogenic acidification. 相似文献
140.
The threshold pH for survival of early life stages of pike Esox lucius in post-mining lakes was determined in a laboratory experiment using artificial water characterized by high concentrations of dissolved Ca, Al, Fe, Mn and SO2/4− . At pH 3·50–4·00, high mortality was observed before hatching. At pH 4·00 and 4·25, hatching rates were reduced compared with the controls (pH 7·40), and many embryos died in a partly hatched state. Hatching success of embryos exposed to pH levels of 4·50 or higher was not affected. However, at pH 5·00 many newly hatched embryos were deformed. Furthermore, pike exposed to pH 4·00–5·00 did not start feeding. At pH 4·75 and below, mortality increased to 100%, and at pH 5·00, only few eleutheroembryos which were in extremely poor condition survived to the end of the experiment. At pH 5·50, survival was in the same range as in the control group, but growth was reduced. Therefore, early life stages of pike are expected to survive in Lustian post-mining lakes when a pH of 5·50 and above is reached and maintained. 相似文献