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61.
Itai Dov Tkacz Sachin Kumar Gupta Vadim Volkov Mali Romano Tomer Haham Pawel Tulinski Ilana Lebenthal Shulamit Michaeli 《The Journal of biological chemistry》2010,285(36):27982-27999
In trypanosomatids, all mRNAs are processed via trans-splicing, although cis-splicing also occurs. In trans-splicing, a common small exon, the spliced leader (SL), which is derived from a small SL RNA species, is added to all mRNAs. Sm and Lsm proteins are core proteins that bind to U snRNAs and are essential for both these splicing processes. In this study, SmD3- and Lsm3-associated complexes were purified to homogeneity from Leishmania tarentolae. The purified complexes were analyzed by mass spectrometry, and 54 and 39 proteins were purified from SmD3 and Lsm complexes, respectively. Interestingly, among the proteins purified from Lsm3, no mRNA degradation factors were detected, as in Lsm complexes from other eukaryotes. The U1A complex was purified and mass spectrometry analysis identified, in addition to U1 small nuclear ribonucleoprotein (snRNP) proteins, additional co-purified proteins, including the polyadenylation factor CPSF73. Defects observed in cells silenced for U1 snRNP proteins suggest that the U1 snRNP functions exclusively in cis-splicing, although U1A also participates in polyadenylation and affects trans-splicing. The study characterized several trypanosome-specific nuclear factors involved in snRNP biogenesis, whose function was elucidated in Trypanosoma brucei. Conserved factors, such as PRP19, which functions at the heart of every cis-spliceosome, also affect SL RNA modification; GEMIN2, a protein associated with SMN (survival of motor neurons) and implicated in selective association of U snRNA with core Sm proteins in trypanosomes, is a master regulator of snRNP assembly. This study demonstrates the existence of trypanosomatid-specific splicing factors but also that conserved snRNP proteins possess trypanosome-specific functions. 相似文献
62.
The nuclear LSm2-8 (like Sm) complex and the cytoplasmic LSm1-7 complex play a central role in mRNA splicing and degradation, respectively. The LSm proteins are related to the spliceosomal Sm proteins that form a heteroheptameric ring around small nuclear RNA. The assembly process of the heptameric Sm complex is well established and involves several smaller Sm assembly intermediates. The assembly of the LSm complex, however, is less well studied. Here, we solved the 2.5 Å-resolution structure of the LSm assembly intermediate that contains LSm5, LSm6, and LSm7. The three monomers display the canonical Sm fold and arrange into a hexameric LSm657-657 ring. We show that the order of the LSm proteins within the ring is consistent with the order of the related SmE, SmF, and SmG proteins in the heptameric Sm ring. Nonetheless, differences in RNA binding pockets prevent the prediction of the nucleotide binding preferences of the LSm complexes. Using high-resolution NMR spectroscopy, we confirm that LSm5, LSm6, and LSm7 also assemble into a 60-kDa hexameric ring in solution. With a combination of pull-down and NMR experiments, we show that the LSm657 complex can incorporate LSm23 in order to assemble further towards native LSm rings. Interestingly, we find that the NMR spectra of the LSm57, LSm657-657, and LSm23-657 complexes differ significantly, suggesting that the angles between the LSm building blocks change depending on the ring size of the complex. In summary, our results identify LSm657 as a plastic and functional building block on the assembly route towards the LSm1-7 and LSm2-8 complexes. 相似文献
63.
报道了广西蕨类植物一新记录属——藤蕨科网藤蕨属LomagrammaJ.Sm.;经野外观察及标本研究,将云南网藤蕨L.yunnanensis Ching处理为网藤蕨L.matthewii(Ching)Holttum的异名,并绘制了墨线图以便于分类识别。 相似文献
64.
《Molecular cell》2021,81(17):3481-3495.e7
65.
G F Crouse 《Gene》1985,40(1):151-155
A plasmid, pgam, has been constructed which expresses the phage lambda gene, gam, under the control of the lambda late promoter, p'R, contained in a form of a p'R-qut-t'R1 module. Lambda red- gam-, which normally do not grow on recA- hosts, are able to grow on recA- hosts containing pgam, because their Q function can turn on the gam gene expression. This facilitates cloning with lambda red- gam- vectors in recA- hosts. 相似文献
66.
67.
Synthesis and photoluminescence characteristics of Sm3+‐doped Bi4Si3O12 red‐emitting phosphor 下载免费PDF全文
A series of novel red‐emitting Sm3+‐doped bismuth silicate phosphors, Bi4Si3O12:xSm3+ (0.01 ≤ x ≤ 0.06), were prepared via the sol–gel route. The phase of the synthesized samples calcinated at 800 °C is isostructural with Bi4Si3O12 according to X‐ray diffraction results. Under excitation with 405 nm light, some typical peaks of Sm3+ ions centered at 566, 609, 655 and 715 nm are found in the emission spectra of the Sm3+‐doped Bi4Si3O12 phosphors. The strongest peak located at 609 nm is due to 4G5/2–6H7/2 transition of Sm3+. The luminescence intensity reaches its maximum value when the Sm3+ ion content is 4 mol%. The results suggest that Bi4Si3O12:Sm3+ may be a potential red phosphor for white light‐emitting diodes. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
68.
Pacheco LG Mati VL Castro TL Dorella FA Oliveira SC Miyoshi A de Melo AL Azevedo V 《Parasitology international》2008,57(4):506-508
The protection against Schistosoma mansoni infection was evaluated in SWISS mice orally vaccinated with an attenuated strain of Salmonella carrying a Sm14-based DNA vaccine. Although this formulation was not able to afford a reduction in the worm burden, a non-antigen-specific decrease in schistosome-induced granulomatous reaction was verified in livers of mice that received Salmonella. 相似文献
69.
The nature of nucleolar associate bodies in the meristematic cells of the root of Lupinus luteus L. was investigated using immunocytochemical methods, in situ hybridisation with light, confocal, and electron microscopy. The nuclear bodies of lupin proved to be structures containing
fibrillarin and coilin, but devoid of rRNA and DNA, like animal coiled bodies (CBs). In lupin cells we have observed the occurrence
of small nuclear ribonucleoprotein (snRNP) in the cytoplasm, in nucleoplasm, CBs and in nucleoli. This type of snRNP localisation
pattern is in agreement with recently presented models of the small nuclear RNA cycle.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
70.