四川阿坝产野生大花红景天不同器官中红景天苷含量的比较

大花红景天[Rhodiola crenulata（Hook.f.et Thoms）H．Ohba]为药材红景天的基原植物,是珍稀的药食兼用天然植物资源之一[1-2]。红景天具有益气活血、通脉平喘、扶正固本的作用[1],主要含黄酮类、有机酸类、多糖类、苷类、香豆素类和挥发性成分等,其中包含的重要生物活性成分有红景天苷、红景天素、酪醇、二苯甲基六氢吡啶和超氧化物歧化酶（SOD）等,还含有维生素及人体必需的多种大量和微量元素,营养价值极高[3-5]。红景天具有抗疲劳、抗缺氧、强壮机体、抗心肌缺血、抗肿瘤和保护神经细胞等作用,红景天药材或其提取物已成为多种中成药、食品、饮料和化妆品的重要原料旧[6]。     

唐古拉红景天防治肝纤维化机制研究

目的：探讨唐古拉红景天对肝纤维化的治疗作用,并探讨作用机制。方法：将60只wistar大鼠随机分为正常组、模型组和红景天治疗组。采用CCL4复合造模法造成肝纤维化,同时进行不同药物治疗,6周后采血并处死动物取材做病理学检查。采用免疫组化检测肝组织中Fas、FasL和NF-кB蛋白含量的表达,采用放射免疫法检测血清LN和FN的含量。结果：模型组肝小叶结构紊乱,出现脂肪变性及大量气球样变,中央静脉及汇管区周围胶原纤维组织明显增多。正常组和治疗组肝小叶结构规则。正常组肝组织Fas、FasL蛋白呈棕黄色弱阳性表达,模型组Fas、FasL蛋白较正常组明显增强（P〈0.05）;治疗组Fas、FasL蛋白的表达均较模型组有明显的减弱（P〈0.05）;正常组肝组织NF-κB蛋白呈棕黄色阳性强表达,模型组NF-κB蛋白的表达较正常组明显减弱（P〈0.05）;治疗组NF-κB蛋白的表达较模型组有明显的增强（P〈0.05）;模型组血清Ⅲ型前胶原氨基端肽（PⅢNP）、LN的表达较正常组明显增强（P〈0.05）;治疗组血清PⅢNP、LN的表达均较模型组有明显的减弱（P〈0.05）。结论：唐古拉红景天通过抑制肝细胞的凋亡对肝纤维化具有一定的防治作用。     

红景天类药物治疗心绞痛的系统评价

目的：评价红景天类药物治疗心绞痛的疗效和不良反应。方法：电子检索MEDLINE、EMBASE、中国生物医学文献数据库、中文科技期刊全文数据库和中文学术期刊全文数据库,并手工检索相关杂志,检索日期截止至2006年2月。纳入以心绞痛患者为研究对象、比较红景天类药物与其它药物治疗效果的随机对照试验。评价纳入研究的质量,并用RevMan4．2．8软件进行Meta分析。结果：共纳入8个研究,合计样本量894例。红景天类药物在心绞痛临床症状、心电图、中医证候及硝酸甘油停减率方面的疗效均优于其它治疗用药,其汇总OR分别为2．44（95％CI1．67—3．58）、1．42（95％CI1．06—1．89）、2．04（95％CI1．27—3．29）和1．88（95％CI1．25—2．83）。未发现明显的不良反应。结论：红景天类药物在心绞痛临床症状、心电图、中医证候及硝酸甘油停减率方面的疗效显示出比其它治疗用药更加有效的倾向,尚需更多高质量研究以增加证据的强度。     

土壤水分对高山红景天生长和红景天甙含量的影响

温室中3种土壤水分条件(土壤相对含水量分别为35％～55％、55％～75％和75％～95％)下,以55％～75％土壤相对含水量上生长的高山红景天的株高、生物量、红景天甙含量和产量最高。     

Genetic structure of endangered Rhodiola sachalinensis

Rh. sachalinensis is a perennial,medicinal herb. The species is rare with apatchy distribution. Due to heavy disturbanceby human activities, it is now endangered.Allozyme analysis was employed to estimate itsgenetic diversity. Nineteen loci encoding 12enzyme systems were used. Rh.sachalinensis has a lower genetic variationwithin patches and higher differentiationbetween patches than other endangered, endemicspecies with limited distributions. The geneticdifferentiation between patches withingeographic regions is lower than that amonggeographic regions. Genetic drift is likely adominant factor contributing to the observedpattern of genetic structure. Because a higherdegree of genetic differentiation existsbetween patches in Rh. sachalinensis,more populations should be protected insitu, and samples from more populations shouldbe collected for ex situ conservation.     

长鞭红景天中一个新的葡萄糖甙

从云南怒江产的长鞭红景天 (Rhodiolafastigiata (Hook .f.etThoms.)S .H .Fu)根茎中分离得到 13个化合物 ,它们的结构通过波谱和化学方法得到鉴定。其中 ,化合物 1被鉴定为新的葡萄糖甙 (2_O_β_D_吡喃葡糖基_3_甲基_戊酸甲酯 ) ,命名为长鞭红景天素甲 ,化合物 2和 5～ 9首次从该植物中分离得到     

狭叶红景天幼苗对水分及遮阴的生长及生理生化响应

研究植物对水分和遮阴胁迫的响应及其生理机制对制定合理的栽培管理措施十分必要。以红景天属植物为研究对象,设置土壤含水量分别为田间持水量的80%(过湿水分)、70%(正常水分)、60%(轻度干旱)、40%(中度干旱)、20%(重度干旱)5个水分梯度;设置2个遮阴处理,以全光照(遮阴率为0)为对照、黑色遮阴网遮阴(遮阴率为85%),研究狭叶红景天生长及生理生化指标的变化特征。结果表明:在不同水分处理下,与对照相比,叶绿素含量、茎干重和茎重比(SMR)显著增加(P0.05),株高、总生物量、叶面积、叶干重、叶重比(LMR)、比叶面积(SLA)、叶面积比(LAR)和叶面积根干重比(LARMR)增加,根冠比和根重比(RMR)减少;随着干旱程度加剧,丙二醛(MDA)、脯氨酸(Pro)和可溶性糖(Ss)含量增加,超氧化物歧化酶(SOD)活性总体呈先增加后减小的趋势。在遮阴处理下,株高、SMR、SLA、LAR和LARMR显著增加(P0.05),叶绿素SPAD值和叶面积增加,总生物量、根干重、根冠比和LMR显著减少(P0.05),茎干重和叶干重减少,MDA含量显著增加,Pro含量略有下降,Ss含量减少。在水分胁迫下,狭叶红景天中度干旱时通过增加酶活性抵御伤害,重度干旱超过其阈值,SOD活性下降,植物体受到伤害,Ss可能是主要的渗透调节物质。在遮阴处理下,狭叶红景天通过增加SLA避免遮阴伤害。狭叶红景天在受到环境胁迫时会通过形态改变、调节MDA含量、抗氧化酶活性和渗透调节物质来保证自身正常的生长发育。     

Specific Characteristics of Rhodiola rosea Growth and Development under the Photoculture Conditions

Growth and development of Rhodiola rosea L. plants (the family Crassulaceae) were compared in their natural habitat, field stands, and in photoculture. By the indices of growth and development, plants grown for 135–137 days under the intensive photoculture were shown to exceed the 3-year-old plants developed in the natural habitats and 1–1.5-year-old plants grown in the field stands. Under the photoculture, 35% of all the plants under study started flowering at the day 75–77 after seed germination. The content of salidroside in the rhizomes of the 135–137-day-old plants was 0.4–0.6% per dry weight. Following photoculturing for 245 days, rhizome weight increased 4.5-fold as compared to the 135–137-day-old plants, and the salidroside concentration reached 1.2%, the level corresponding to the maximum content of this glycoside in the plants growing in their natural habitat and exceeding by 1.5–3 times the levels observed in the plants grown in the field stands. Under the photoculture conditions, plants of R. rosea were shown to grow without the dormancy period. Several factors apparently raised the salidroside concentration in the 245-day-old plants under the photoculture conditions, including enhanced growth, absence of the dormancy period and the period of lowered temperatures; as a whole, these factors promoted the detoxification, storage, and/or transport of the primary metabolic products.     

The golden root, Rhodiola rosea, prolongs lifespan but decreases oxidative stress resistance in yeast Saccharomyces cerevisiae

The effect of aqueous extract from R. rosea root on lifespan and the activity of antioxidant enzymes in budding yeast Saccharomyces cerevisiae have been studied. The supplementation of the growth medium with R. rosea extract decreased survival of exponentially growing S. cerevisiae cells under H₂O₂-induced oxidative stress, but increased viability and reproduction success of yeast cells in stationary phase. The extract did not significantly affect catalase activity and decreased SOD activity in chronologically aged yeast population. These results suggest that R. rosea acts as a stressor for S. cerevisiae cells, what sensitizes yeast cells to oxidative stress at exponential phase, but induces adaptation in stationary phase cells demonstrating the positive effect on yeast survival without activation of major antioxidant enzymes.