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21.
Use of the additive main effects and multiplicative interaction model in QTL mapping for adaptation in barley 总被引:4,自引:0,他引:4
Ignacio Romagosa Steven E. Ullrich Feng Han Patrick M. Hayes 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(1-2):30-37
The additive main effects and multiplicative interaction (AMMI) model has emerged as a powerful analytical tool for genotype x environment studies. The objective of the present study was to assess its value in quantitative trait locus (QTL) mapping. This was done through the analysis of a large two-way table of genotype-by-environment data of barley (Hordeum vulgare L.) grain yields, where the genotypes constituted a genetic population suitable for mapping studies. Grain yield data of 150 doubled haploid lines derived from the Steptoe x Morex cross, and the two parental lines, were taken by the North American Barley Genome Mapping Project (NABGMP) at 16 environments throughout the barley production areas of the USA and Canada. Four regions of the genome were responsible for most of the differential genotypic expression across environments. They accounted for approximately 50% of the genotypic main effect and 30% of the genotype x environment interaction (GE) sums of squares. The magnitude and sign of AMMI scores for genotypes and sites facilitate inferences about specific interactions. The parallel use of classification (cluster analysis of environments) and ordination (principal component analysis of GE matrix) techniques allowed most of the variation present in the genotype x environment matrix to be summarized in just a few dimensions, specifically four QTLs showing differential adaptation to four clusters of environments. Thus, AMMI genotypic scores, when the genotypes constituted a population suitable for QTL mapping, could provide an adequate way of resolving the magnitude and nature of QTL x environment interactions.Ignacio Romagosa was on sabbatical leave from the University of Lleida and the Institut de Recerca i Tecnologia Agroalimentàries, Lleida, Spain, when this study was conducted 相似文献
22.
纯系间数量性状主基因差异的遗传分析 总被引:5,自引:0,他引:5
将Elston模型应用于存在主基因差异的系间杂交的分离世代分析,提出利用似然函数分析数量性状的尺度效应、主基因分离比例以及主基因效应和微基因效应的方法,并应用于水稻遗传实验,结果表明,半矮秆籼稻品种南京11号带有的隐性矮秆主基因,效应大约为40-56cm,微基因效应以加性为主,且主、微型基因存在显著互作,但互作和微基因效应均远小于基因效应。 相似文献
23.
Abstract: The NMDA receptor exhibits increased sensitivity to stimulation during early development compared with the adult. In this study, we examined modulation of the NMDA receptor by polyamines during development to see if it correlates with differences in the functional responsiveness of the NMDA receptor. [3H]MK-801 binding was measured in discrete brain regions in the presence and absence of polyamines in 3-, 7-, 15-, 25-, and 60-day-old Sprague-Dawley rats. [3H]MK-801 binding increased between postnatal days 3 and 15, with adult levels of binding being reached between days 15 and 25. Spermidine (75 μM) caused maximal stimulation of [3H]MK-801 binding during early development, ranging from 250% in the thalamus to 450% in the caudate putamen at postnatal day 3. This effect gradually declined to levels seen in the adult by postnatal days 15–25. During all developmental stages, the stimulation seen was greater in the caudate putamen compared with the hippocampus. Diethylenetriamine (1 μM) exhibited similar developmental and regional heterogeneity in its effects on [3H]MK-801 binding, producing substantial stimulation of binding in the neonate, but not in the adult. The EC50 and Emax values for the stimulatory effect of spermidine were significantly higher at day 7 compared with the adult. Unlike spermidine and diethylenetriamine, there was no regional variation in the effects of the putative “polyamine site” inverse agonist 1,10-diaminodecane at any age and only a slightly attenuated inhibition at postnatal day 3 compared with the adult. This lack of complementarity in the regional and developmental profiles of spermidine and diethylenetriamine, on the one hand, and 1,10-diaminodecane, on the other, suggests that their effects on [3H]MK-801 binding are mediated at different sites. The altered sensitivity of the NMDA receptor to polyamines during development could reflect the expression of molecular variants with different sensitivities to modulation by polyamines. 相似文献
24.
R. Wu R. F. Stettler 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(7-8):1046-1054
One approach to gain an insight into the genetics of tree architecture is to make use of morphologically divergent parents and study their segregating progeny in the F2 and backcross (B1) generations. This approach was chosen in the present study in which material of a three-generation pedigree growing side by side in a replicated plantation, was analyzed. The pedigree included Populus trichocarpa (T) and P. deltoides (D) parents, their F1 and F2 hybrids and their B1 hybrids to the D parent. The trees were grown in the environment of the T parent and measured for the first 2 years of growth. Nine quantitative traits were studied at the stem, branch and leaf levels of tree architecture, in which the original parents differed. Strong F1 hybrid vigor relative to the better parent (T) was expressed in growth and its components. Most quantitative traits in the F2 and B1 hybrids were intermediate between the T and D parents but displayed a wide range of variation due to segregation. The results from the analysis of variance indicated that all morphometric traits were significantly different among F2 and B1 clones, but the B1 hybrids were more sensitive to replicates than the F2. Broad-sense heritabilities (H
2) based on clonal means ranged from moderately high to high (0.50–0.90) for the traits studied, with H
2 values varying over age. The H
2 estimates reflected greater environmental noise in the B1 than in the F2, presumably due to the greater proportion of maladaptive D alleles in those hybrids. In both families, sylleptic branch number and length, and leaf size on the terminal, showed strong genetic correlations with stem growth. The large divergence between the two original parents in the traits studied, combined with the high chromosome number in Populus (2n=38), makes this pedigree well suited for the estimation of the number of quantitative trait loci (QTLs) underlying quantitative variation by Wright's biometric method (1968). Variation in several traits was found to be under the control of surprisingly few major QTLs: 3–4 in 2nd-year height and diameter growth, a single QTL in stem diameter/height ratio. 相似文献
25.
M. J. Kearsey V. Hyne 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(6):698-702
A method to locate quantitative trait loci (QTL) on a chromosome and to estimate their additive and dominance effects is described. It applies to generations derived from an F1 by selfing or backcrossing and to doubled haploid lines, given that marker genotype information (RFLP, RAPD, etc.) and quantitative trait data are available. The method involves regressing the additive difference between marker genotype means at a locus against a function of the recombination frequency between that locus and a putative QTL. A QTL is located, as by other regression methods, at that point where the residual mean square is minimised. The estimates of location and gene effects are consistent and as reliable as conventional flanking-marker methods. Further applications include the ability to test for the presence of two, or more, linked QTL and to compare different crosses for the presence of common QTL. Furthermore, the technique is straightforward and may be programmed using standard pc-based statistical software. 相似文献
26.
W. C. Kennard M. K. Slocum S. S. Figdore T. C. Osborn 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,87(6):721-732
A cross between the open-pollinated Brassica oleracea cabbage cultivar Wisconsin Golden Acre and the hybrid broccoli cultivar Packman was used with molecular markers to investigate the genetic control of morphological variation. Twenty-two traits derived from leaf, stem, and flowering measurements were analyzed in 90 F2 individuals that were also classified for genotype by restriction fragment length polymorphism (RFLP) markers. Seventy-two RFLP loci, which covered the mapped genome at an average of 10 map-unit intervals on all nine linkage groups, were tested individually for associations to phenotypic measurements by single factor ANOVA, and markers with significant associations (P<0.05) were used to develop multilocus models. These data were utilized to describe the location, parental contribution of alleles, magnitude of effect, and the gene action of trait loci. Single marker loci that were significantly associated (P<0.05) with trait measurements accounted for 6.7–42.7% of the phenotypic variation. Multilocus models described as much as 60.1% of the phenotypic variation for a given trait. In some cases, different related traits had common marker-locus associations with similar gene action and genotypic class ranking. The numbers, action, and linkages, of genes controlling traits estimated with marker loci in this population corresponded to estimates based on classical genetic methods from other studies using similar, or similarly-wide, crosses. There was no evidence that genome duplication accounted for a significant portion of multiple genes controlling trait loci over the entire genome, but possible duplications of trait loci were identified for two regions with linked, duplicated marker loci. 相似文献
27.
Location of a gene regulating drought-induced abscisic acid production on the long arm of chromosome 5A of wheat 总被引:3,自引:0,他引:3
S. A. Quarrie M. Gulli C. Calestani A. Steed N. Marmiroli 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(6):794-800
The accumulation of abscisic acid (ABA) by detached and partially dehydrated wheat leaves is known to be inherited in a quantitative manner. The location of genes having a major effect on drought-induced ABA accumulation in wheat was determined using a set of single chromosome substitution lines and populations derived from a cross between a high-ABA- and a low-ABA-producing genotype. Examination of a series of chromosome substitution lines of the high-ABA genotype Ciano 67 into the low-ABA recipient Chinese Spring showed that chromosome 5A carries gene(s) that have a major influence on ABA accumulation in a drought test with detached and partially dehydrated leaves (DLT). A similar DLT was used to examine ABA accumulation in a population of F2 plants and doubled haploid (DH) lines derived from the cross between Chinese Spring (low-ABA) and SQ1 (high-ABA) in which the F2 population (139 plants) and DH lines (96 lines) were also mapped partially with molecular markers. Analysis of variance of ABA accumulation between and within marker allele classes in the F2 confirmed the location of a gene(s) regulating ABA accumulation on the long arm of chromosome 5A. MAPMAKERQTL showed the most likely position for the ABA quantitative trait locus (QTL) to be between the loci Xpsr575 and Xpsr426, about 8 cM from Xpsr426. A similar trend for high ABA accumulation was found in DH lines having the SQ1 allele at marker loci in the same region of chromosome 5AL, but the QTL effect was not significant. The function of the QTL is discussed. 相似文献
28.
Linkage disequilibrium in crosses between Illinois maize strains divergently selected for protein percentage 总被引:1,自引:0,他引:1
J. W. Dudley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,87(8):1016-1020
The objectives of this study were two fold: (1) to determine whether divergent selection for kernel protein concentration, which produced the Illinois high protein (IHP), Illinois low protein (ILP), reverse low protein (RLP), and reverse high protein (RHP) maize (Zea mays L.) strains, had generated coupling-phase linkages among genes controlling protein concentration or other traits and (2) to measure the effectiveness of random mating in reducing linkage disequilibrium in segregating generations from crosses between the strains. To achieve these objectives, design III progenies from the F2 and F6 (produced by random mating the F2) from the crosses of IHP × ILP, IHP × RHP, ILP × RLP, and RHP × RLP were evaluated. Estimates of additive variance for percent protein in the crosses of IHP × ILP and ILP × RLP were significantly less in the F6 than in the F2 indicating the presence of coupling-phase linkages in the parents and their breakup by random mating. In addition, a significant reduction in dominance variance for grain yield from the F2 to the F6 in IHP × ILP suggested the presence of repulsion-phase linkages. No other evidence of coupling or repulsion-phase linkages was found for any of the traits measured. These results demonstrate the effectiveness of long-term divergent selection in the development of coupling-phase linkages and of random mating to dissipate linkage disequilibrium.Research supported by the Illinois Agricultural Experiment Station 相似文献
29.
Bayesian analysis of linkage between genetic markers and quantitative trait loci. I. Prior knowledge 总被引:8,自引:0,他引:8
I. Hoeschele P. M. VanRaden 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,85(8):953-960
Summary Prior information on gene effects at individual quantitative trait loci (QTL) and on recombination rates between marker loci and QTL is derived. The prior distribution of QTL gene effects is assumed to be exponential with major effects less likely than minor ones. The prior probability of linkage between a marker and another single locus is a function of the number and length of chromosomes, and of the map function relating recombination rate to genetic distance among loci. The prior probability of linkage between a marker locus and a quantitative trait depends additionally on the number of detectable QTL, which may be determined from total additive genetic variance and minimum detectable QTL effect. The use of this prior information should improve linkage tests and estimates of QTL effects. 相似文献
30.