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731.
Patients with vitiligo have circulating antibodies directed in part to pigment cell antigens with MWs of approximately 90, 75, and 40-45 kDs. These antigens are denominated VIT 90, VIT 75, and VIT 40, respectively. To further characterize these “vitiligo” antigens, we examined their relation to antigens defined by a panel of 25 monoclonal antibodies (moab) to pigment cell antigens. We found by immunoprecipitation and SDS-PAGE analysis of 125I labelled, detergent soluble, human melanocyte macromolecules, that 24 (83%) of 29 patients with vitiligo had antibodies to one or more vitiligo antigens vs. 2 (7%) of 28 control individuals. Seventeen of the 25 moabs did not react with any labelled antigen in the same lysate. Of the remaining eight moabs, only four precipitated an antigen that co-migrated with one of the vitiligo antigens. Moab TA99, HMSA-5, and TMH-1 (all directed to the 75 kD tyrosinase-related protein [TRP1]) co-migrated with VIT 75. Moab W6/32 (directed to class I HLA antigen) co-migrated with VIT 40. Immunodepletion studies with vitiligo antibodies selectively depleted the antigen defined by W6/32 but not the antigen defined by TA99 and HMSA-5, indicating that VIT 75 was not the 75 kD tyrosinase-related protein. The vitiligo antigens were easily labelled by the lactoperoxidase technique but poorly labelled with 35S-methionine, suggesting they are expressed on the cell surface. These studies indicate that VIT 90 and VIT 75 differ from antigens defined by currently available moabs to pigment cell antigens. VIT 40 appears to share a cross-reactive epitope, or be tightly bound to, class I HLA antigen. 相似文献
732.
E. A. Kingsley Teresa E. Carter Kevin D. Barrow Pamela J. Russell 《Cancer immunology, immunotherapy : CII》1995,41(5):317-323
A monoclonal antibody, BLCA-8, was raised against the human bladder cancer cell line, UCRU-BL-17CL. By flow cytometry and
immunoperoxidase staining, this antibody was found to possess high specificity for bladder tumours, some reactivity with fetal
tissues, and no reactivity with normal bladder, or any normal or malignant tissue. This high specificity and the stability
of the antigen to the urinary environment suggest that BLCA-8 may have potential for use as an anti-bladder-cancer therapeutic
agent. By thin-layer chromatography and autoradiography, BLCA-8 was found to bind four components within the neutral lipid
fraction of a bladder cancer cell line, UCRU-BL-17/23α. These components had R
F values of 0.22, 0.16/0.15 (doublet), 0.12 and 0.08, and migrated below globoside, indicating the presence of more than four
sugars. By enzyme-linked immunosorbant assay and thin-layer chromatography it was found that the binding of BLCA-8 to the
lipid extract was increased by both mild alkaline hydrolysis and enzymatic treatments, indicating that adjacent phospholipids
and glycolipids interfere with the accessibility of the antibody-binding site. Full biochemical characterisation of the BLCA-8
antigen is currently underway.
Received: 24 April 1995 / Accepted: 11 July 1995 相似文献
733.
Klaus B. Gondolf Stephen Batsford Gabriele Lässle Enrico Curschellas Andreas Mertz 《Virchows Archiv. B, Cell pathology including molecular pathology》1991,60(1):353-363
The aims of the present study were to define, under in vivo conditions, factors governing antigen binding and persistence
in the rat joint and to establish a chronic arthritis model by means of a natural polycation.
The influence of size as well as charge on antigen handling was examined using a range of chemically cationized proteins and
natural polycations. Arthritis was induced by intraarticular challenge in preimmunized rats. Immunofluorescence studies revealed
that not only pI, which must exceed pH 8–9, but also molecular size was a decisive parameter: only antigens of more than 40
kD were able to persist for significant periods in joint structures. All existing models of antigen induced chronic arthritis
in rodents utilize chemically cationized proteins. We extended this system to natural polycations by showing that lysozyme
(pI 11.3; MW 14 kD) in tetrameric, charge conserved form (MW 56 kD) as a modelantigen was able to induce chronic arthritis
in the rat. After intraarticular challenge of preimmunized animals the course of inflammation was assessed both by99mTechnetium-pertechnetate (99mTc) scintigram and from the histology. In contrast to monomeric lysozyme, which evoked only a transient inflammatory response
(less than two weeks), tetrameric lysozyme induced a chronic arthritis, which still persisted at day 90. Our results show
that the ability of cationic antigens to trigger chronic arthritis is vitally size dependant. This is also the first report
of a natural polycation acting as an arthritogen, thus providing an experimental basis justifying the search for cationic
microbial antigens in human post infectious reactive arthritis. 相似文献