组织培养暗紫贝母的药理作用

以组织培养的暗紫贝母为材料,野生暗紫贝母为对照,用不同溶剂提取,得４个化学组人 生物碱部分,总皂甙部分,水溶性部分和脂溶性部分,并以生药粉为阳性对照进行药效学试验。结果表明：组培贝母与野生贝母有相似的止咳、祛痰作用;总皂甙部分与总生物碱部分均为川贝有效活性成分,ｔ得之间无显著性差异;经ＴＬＣ是乌头总生物碱部分与总皂甙部分无化学成分重叠。     

阳江地区慢性咳嗽的病因学调查研究

目的探讨阳江地区慢性咳嗽的病因特点。方法对2010年3月～2012年3月在阳江市中医医院门诊就诊的200例慢性咳嗽患者的临床资料进行研究。结果确定病因诊断者187例,单一病因咳嗽者178例,其中前五位依次为上气道咳嗽综合征（占34.0%）,变异型哮喘（占18.0%）,胃食管反流（占13.5%）,变应性咳嗽（占12.5%）,嗜酸细胞性支气管炎（占11.0%）;二重病因9例中,为上气道咳嗽综合征并胃食管反流（占2.5%）,上气道咳嗽综合征合并变异型哮喘（占2.0%）。结论慢性咳嗽的病因复杂,涉及多个临床学科,应进一步诊断及鉴别诊断。     

HPLC法测定复方止咳清肺胶囊中有效成分含量的研究

首次采用高效液相色谱法对中药复方止咳清肺胶囊中的有效成分—芥子碱硫氰酸盐进行了含量测定研究,由此建立了该复方的HPLC法质量分析方法。     

中国百日咳疫苗的现状及研发趋势初探

百日咳是许多国家的严重公共卫生问题,目前预防百日咳最有效和最经济的方法是接种百日咳疫苗,百日咳组分疫苗由于其保护效果好、不良反应低、质量稳定可控而成为新一代无细胞百日咳疫苗的首选。本文简要介绍百日咳的病原学、传播途径及流行病学特点,并对中国的百日咳疫苗研发趋势进行初步探讨。     

Production and secretion of pertussis toxin subunits in Bacillus subtilis

Pertussis toxin (PT) is a major component of today's acellular whooping cough vaccines. The use of acellular vaccines is predicted to increase sharply in the near future. There is therefore a need to produce PT in a way that makes its purification as easy as possible. Our approach was to express all five PT subunits individually in Bacillus subtilis. We have used vectors containing the promoter and signal sequences of the alpha-amylase gene of Bacillus amyloliquefaciens followed by an insert encoding the appropriate PT-subunit. All PT-subunits were secreted and found in the culture supernatant. The level of expression varied considerably: S1 and S5 were produced in large quantities whereas much smaller amounts of S2, S3 and S4 were found. The subunits were also present in the membrane fraction of the respective strains.     

Serum resistance in bvg-regulated mutants of Bordetella pertussis

Serum resistance, or resistance to killing by antibody dependent pathway of complement, in Bordetella pertussis is bvg-regulated and the Bordetella resistance to killing (brk) locus mediates much of the resistance. Here we examined whether other bvg-regulated proteins contribute to serum resistance. We found that neither pertussis toxin, adenylate cyclase toxin, filamentous hemagglutinin, dermonecrotic toxin, tracheal colonization factor, nor Vag8 mutants were sensitive to serum killing compared to the wild-type. Filamentous hemagglutinin has been reported to bind C4 binding protein, an inhibitor of complement, but this activity does not appear to contribute to serum resistance, as evidenced by the resistant phenotype of FHA mutants. Clinical isolates were serum resistant and wild-type strains possessing an additional copy of the brk locus were 2–5-fold more resistant to serum killing.     

咳嗽变异性哮喘（CVA）与小气道功能相关性分析

目的:探讨咳嗽变异性哮喘(CVA)患者小气道功能检查对其诊断,治疗的意义。方法:对254例以慢性咳嗽为主的患者行肺功能检查并行支气管激发试验,回顾性分析小气道病变及气道高反应性检查结果与咳嗽变异性哮喘(CVA)的相关性。结果:有小气道功能障碍者接受吸入乙酰甲胆碱激发试验,气道反应性明显增高。有小气道功能障碍确诊咳嗽变异性哮喘(CVA)组起始阻力、反应阈值及阻力上升度与非哮喘组相比差异有统计学意义(P<0.01)。结论:检查小气道功能障碍有助于哮喘的诊断,治疗及预后随访。     

润肺健脾益肾汤对小儿过敏性咳嗽淋巴细胞亚群绝对计数的影响

目的:探讨润肺健脾益肾汤对小儿过敏性咳嗽淋巴细胞亚群绝对计数的影响。方法:选择自2017年5月至2019年1月在盘锦辽油宝石花医院(我院)儿科就诊的过敏性咳嗽患儿128例,根据随机数字表法把患儿分为中药组与对照组,各64例,对照组给予沙丁胺醇治疗,观察组在对照组治疗对的基础上给予润肺健脾益肾汤治疗,两组都治疗观察14 d,记录淋巴细胞亚群绝对计数变化情况。结果:治疗后中药组的总有效率为98.3%,显著高于对照组的88.3%(P0.05)。两组治疗后的1s用力呼气容积(Forced expiratory volume in one second,FEV1)和1s用力呼气容积肺活量(Forced vital capacity,FVC)值都显著高于治疗前(P0.05),中药组也显著高于对照组(P0.05)。两组治疗后的血清白细胞介素(interleukine,IL)-4、IL-13水平都显著低于治疗前(P0.05),中药组也显著低于对照组(P0.05)。两组治疗后的CD4~+T淋巴细胞绝对计数显著高于治疗前(P0.05),中药组也显著高于对照组(P0.05),两组治疗前后CD8~+T淋巴细胞绝对计数比较无差异(P0.05)。结论:润肺健脾益肾汤治疗小儿过敏性咳嗽能提高CD4~+T淋巴细胞亚群绝对计数水平,抑制炎症因子的表达,从而改善患儿的肺功能,提高治疗效果。     

Growth retardation in weight and catch-up growth following infection by measles and whooping cough among babies of rural Zaire (Lake Tumba)

This study has shown that measles and whooping cough affect growth in weight among babies of rural Zaire (Lake Tumba). The average loss in weight due to measles, is more than twice the amount due to whooping cough. However, the time needed to regain the original growth channel after infection by measles, is about half the time needed to fully catch up after whooping cough. The amount of weight loss is proportional to the child's weight at the onset of the disease. There is no difference in the susceptibility to lose weight after measles or whooping cough, between boys and girls, nor between Oto and Twa. This study has also shown that the effect of measles and whooping cough on the growth in weight, is independent of the season of the hear, although the incidence of these diseases showed a significant seasonal pattern. Finally, this study gives evidence for the fact that the incidence of weight loss one month after the onset of measles or whooping cough is age-related.