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Hydrogen metabolism in blue-green algae. 总被引:12,自引:0,他引:12
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Preillumination of R. rubrum membranes in the presence of 50M phenazine methosulfate produces an inhibition of their photophosphorylating capacity.At low pH's phosphate protects against this photo-inhibition, whereas increasing the pH eliminates this protective effect. The inhibition can be produced not only by preillumination at high pH in the presence of phenazine methosulfate, but also by preillumination at low pH (5.0) and then shifting the pH to 8.0 in the dark. We have also measured the effect of preillumination in the presence of an uncoupler such as carbonylcyanide p-trifluoromethoxyphenylhydrazone, and found that at normal pH's while photophosphorylation was protected, the uncoupler activated ATPase was inhibited pointing to a clear difference for both reactions and perhaps different structural requirements for their activities.The purified coupling factor protein isolated from either normal or photoinactivated membrane will reconstitute normal photophosphorylation in previously uncoupled membranes but not in uncoupled membranes which were inactivated by preillumination with phenazine methosulfate prior to uncoupling. 相似文献
94.
A23187 and certain other carboxylate ionophores are capable of transferring Fe(II) but not Fe(III)1 across phospholipid bilayers (liposomes) and red cell membranes. A23187 is able to transfer Fe(II) from ferritin loaded liposomes when allied with a suitable redox couple and sink. The affinity of A23187 for Fe(II) is approximately five orders of magnitude greater than for Ca2+, as judged by two phase extraction techniques. 相似文献
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