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101.
刺激家兔颈交感神经对颈动脉窦反射的影响 总被引:2,自引:0,他引:2
在36只麻醉家兔观察了电刺激颈交感神经(CSN)对颈动脉窦压力感受器(CSB)活动的影响。所得结果如下:(1)电刺激 CSN 可使夹闭颈动脉引起的加压反射消失或倒转,△BP 从刺激前的 39.5±3.6mmHg 变为刺激时的-0.31±5.4mmHg(P<0.001)。(2)在电刺激CSN 时,静注新福林所诱发的颈动脉窦压力感受器-心率反射增强,表现为反射性心率减慢较刺激前更为明显。(3)在以50—200mmHg 的压力充胀两侧颈动脉窦的条件下,刺激 CSN 引起窦内压与平均动脉压的关系曲线下移,与刺激前曲线相比有明显差异(P<0.01)。(4)切断 CSN 后,动脉血压有所升高,提示 CSN 对 CSB 活动有紧张性调节作用。以上结果比较明确地表明家兔 CSN 对 CSB 活动有调节作用。此作用可能是 CSN 作用于窦壁平滑肌而间接引起的。 相似文献
102.
电针和吗啡对伤害性刺激猫内脏大神经引起的海马诱发电位的影响 总被引:1,自引:0,他引:1
在氯醛糖麻醉并箭毒化的50只猫中,观察了强电流刺激内脏大神经时的背侧海马诱发电位(Hippocampal evoked potential,HEP)以及电针和吗啡对其影响。结果显示,用20V电压刺激内脏大神经可以兴奋包括 Aδ和 C 纤维在内的几乎全部纤维,并且可以在海马内记录到波形稳定的 HEP。以负波为主的 HEP 在海马锥体细胞层附近以及下脚复合体的表浅层记录到,以正波为主的 HEP 在海马锥体细胞顶树突的中下部记录明显。只有刺激强度达到兴奋内脏大神经的 Aδ纤维时才可记录出 HEP。吗啡对 HEP 有显著的抑制作用。电针也可以明显抑制 HEP 的振幅,并有后作用。上述结果提示,内脏大神经中的感觉纤维,包括痛纤维的冲动可以传至海马。 相似文献
103.
104.
Abstract The effect of culture filtrate containing cholera toxin (CT) on rat intestinal permeability was studied using fluorescein isothiocyanate-labelled dextran 3000 (FITC-D3, M r , 3000) as probe molecule. CT was given either perorally, via a gastric tube 90 min before, or locally in conjunction with the permeability measurement in the distal ileum. Compaired to the control animals, either mode of administration resulted in increased permeation of FITC-D3 from the intestine to portal blood. The effect of the local treatment was apparent after 5–10 min and prevailed during the 60-min measurement period. The results indicate that CT not only affects net water transport at the intestinal mucosa but also the passage of larger molecules across the intestinal wall. 相似文献
105.
A model of a myelinated nerve axon is developed on the basis of FitzHugh-Nagumo dynamics under the assumption that the nodes of Ranvier are of small but finite width. It is shown that a periodic excited state may not exist if the width of the nodes is too small and the leakage across the myelin sheath is too great. The propagation of a super threshold pulse is prevented in the absence of nodes. Global stability of the resting equilibrium state is investigated as well as the propagation of wave front, type solutions. 相似文献
106.
Koji Yamada Masumi Ohtsu Genki Kimura 《In vitro cellular & developmental biology. Plant》1985,21(8):428-432
Summary Sodium butyrate causes proliferation arrest with a G2 (4C) DNA content and induces formation of tetraploid cells upon removal
of the inhibitor, in rat 3Y1 diploid fibroblasts. We isolated tetraploid clones from the butyrate-treated 3Y1 cells with high
efficiency; among 21 clones randomly isolated, 5 were pure diploid, 7 were mainly tetraploid with a small contaminating diploid
population, and 7 were pure tetraploid. Among the pure tetraploid clones, two showed doubled chromosome numbers with slightly
broader distributions than that seen in parental 3Y1 cells. Butyrate further induced polyploid formation in the tetraploid
cells thus produced, but octaploid cells that resulted could not be maintained for prolongeed, cultivation. We found no difference
between the tetraploid and the (parental and parallel isolated) diploid clones in terms of colony-forming ability, proliferation
rate, and sensitivity to density-dependent inhibition of proliferation. These results suggest that doubling of chromosome
number by itself does not cause a change in proliferation property. The tetraploid clones had lower average saturation densities
possibly due to enlargement of cell size represented by higher cellular protein content. 相似文献
107.
Barbara A. Brennessel Kathleen J. Keyes 《In vitro cellular & developmental biology. Plant》1985,21(7):402-408
Summary The artificial sweetener saccharin inhibits binding of epidermal growth factor (EGF) to cultured rat pituitary tumor cells
(GH4C1 cells). Saccharin also causes morphological alterations in these cells, resulting in pronounced elongation, stretching, and
firmer attachment of cells to the culture dishes. These alterations in cell shape are similar to those observed after treatment
of GH4C1 cells with EGF and with thyrotropin-releasing hormone (TRH), both of which enhance prolactin (PRL) production in these cells.
After assaying for PRL in saccharin-treated cultures, it was observed that this sweetener is also capable of stimulating PRL
production two-to sixfold in a dose-dependent manner. Enhancement of PRL production can be observed at 0.5 mM saccharin, yet this is 10 times less than the saccharin concentration required to alter cell shape. These effects of saccharin
on cell morphology and on PRL production are reversible in GH4C1 cell cultures. When added to cultures along with maximal concentrations of EGF or TRH, the effects of saccharin on PRL production
are additive, suggesting that the actions of saccharin are mediated by a somewhat different pathway from that of the peptide
hormones. Pulse labeling studies indicate that the enhancement of PRL production is highly specific inasmuch as saccharin
was found to decrease the overall rate of protein synthesis in these cells. Saccharin also causes a decrease in the rate of
DNA synthesis under these treatment conditions. Mitomycin C, which similarly inhibited DNA synthesis, had no effect on cell
morphology or PRL production.
This investigation was supported by a Faculty Research Grant from Wheaton College 相似文献
108.
The first comparative profiles of UDP-glucuronosyltransferase(s) (UDPGT) activities obtained under standard conditions in vitro in mammals (man, rat [Wistar and Gunn], mouse, monkey [Papio papio and Cynomolgus], pig, guinea pig, rabbit, dog) are presented for 16 aglycones. A decreasing scale of these activities was obtained from planar to bulky molecules. The scale was identical for each of the mammals studied, including man. Statistical analysis of the results revealed a division of the aglycones into three groups, one being correlated with the molecular form called GT1 the two others with the GT2 form. The profile of activities in the Gunn rat revealed very weak activity towards planar molecules (GT1). These results provide evidence that under standard conditions, human UDPGT activities are comparable to those from other animals. 相似文献
109.
Faye S. Silverstein Steven Donn Karen Buchanan Michael V. Johnston 《Journal of neurochemistry》1984,43(6):1769-1772
To assess maturation of central serotonin and catecholamine pathways at birth, we measured lumbar CSF homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), stable acid metabolites of dopamine and serotonin, using HPLC with electrochemical detection. CSFs from 57 neonates (38 premature and 19 at term) and 13 infants 1-6 months old were studied. HVA levels increased with maturity (p less than 0.05; ANOVA), whereas 5-HIAA levels were similar in all these subjects. HVA/5-HIAA ratios increased markedly from 1 +/- 0.12 in the most premature neonates to 1.98 +/- 0.17 in the older infants (p less than 0.01; t test). There were no sex differences for these values. 相似文献
110.
A method is described for the extraction of enkephalin-like peptides from peripheral nerve using chloroform and acidic methanol to facilitate a differential extraction of peptides and lipid. Porcine splanchnic nerve contains enkephalin-like peptides in low amounts compared to porcine adrenal medulla and striatum. Gel filtration chromatography reveals the presence of enkephalin-like peptides in both processed and cryptic forms. This is the first reported isolation and partial characterization of these peptides in splanchnic nerve. The presence of these peptides in this nerve provides support for the contention that the splanchnic nerve can modulate catecholamine release from the adrenal medulla through an effect on opiate receptors located on chromaffin cells. 相似文献