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61.
62.
P. J. Cáceres C. A. Faúndez B. Matsuhiro J. A. Vasquez 《Journal of applied phycology》1996,8(6):523-527
The second-derivative mode of the Fourier transform I.R. spectra of dried algal material has been applied to distinguish the carrageenans-producingStenogramme interrupta from the isomorphous speciesRhodymenia howeana. Spectra of the tetrasporophyteS. interrupta showed bands assigned to a -carrageenan type polysaccharide, while the gametophytic and cystocarpic plants showed the characteristic absorptions of -and -carrageenans. Results were confirmed by hot water extraction of samples of the three nuclear phases ofS. interrupta and characterization of the extracts by chemical analysis.Author for correspondence 相似文献
63.
Synchronously dividing binuclear cells were induced in root tips ofTriticum turgidum by caffeine treatment. Spindle and other microtubular configurations of such cells were studied using tubulin immunofluorescence
and electron microscopy. The binuclear cells developed one, two or three preprophase microtubule bands longitudinally, transversely
or rarely in a cross configuration. During the mitotic entry binuclear cells formed prophase spindles separately around each
nucleus. When the nuclei were located fairly apart, their spindle structures developed independently throughout all mitotic
phases. But when the nuclei were located closely together their metaphase and anaphase spindles shared a common polar region.
However, the two spindles in such cells retained their functional autonomy. They display structurally independent minipoles
in the common polar region. After anaphase the neighbouring nonsister chromosome groups of nuclei divided by a common polar
region come to lie close together and in telophase, become enclosed by a common nuclear envelope. During cytokinesis of binuclear
cells cell plates were formed only between sister nuclei. These cell plates may develop normally or may curve or branch giving
rise to aberrant daughter cell walls. The peculiar mode of spindle and spindle polar region organization of binuclear cells
and determination of the division plane in them are discussed. 相似文献
64.
一个恢复力受单基因控制的水稻CMS育性回复突变体 总被引:3,自引:0,他引:3
利用 ̄(60)Co-γ射线对具有印尼水田谷细胞质的籼稻细胞质雄性不育系Ⅱ-32A干种子进行诱变处理,获得了一育性回复突变体T24。育性基因未纯合的突变体分离出可育株和完全不育株,比例为3∶1;其与Ⅱ-32A和珍汕97A测交,F1代分离出1∶1的可育株和不育株。育性稳定株系与Ⅱ-32A和珍汕97A杂交,F2分离成3∶1的可育株和不育株。表明其育性回复是由一对基因显性突变所致。这一突变体对不育系的育性恢复机制不同于明恢63、20964等恢复系,后者表现为两对显性恢复基因作用。未观察到T24与亲本Ⅱ-32A除育性以外的其他性状的差异,因而两者构成育性恢复基因的近等基因系。本文还对不育系育性回复类型和T24的理论意义与育种价值进行了讨论。 相似文献
65.
Eric Dufour Paul Robert Dominique Bertrand Tomasz Haertlé 《Journal of Protein Chemistry》1994,13(2):143-149
Fourier transform infrared spectroscopy has been applied to investigate the secondary structural changes of-lactoglobulin in water/ethanol mixtures. The studies were carried out at two differentpHs and at high protein concentrations. The spectra were recorded using an attenuated total reflection cell. The amide I band of-lactoglobulin in water reveals large amounts of intra extended-sheet structure. About 20% ethanol,-lactoglobulin unfolds and-strand formation is observed.-Helices are built up by increasing the ethanol concentration up to 30%. In 50% ethanol,-lactoglobulin gels providing the apparent pH are neutral. The secondary structural changes of-lactoglobulin were observed on the similarity maps obtained by Principal Component Analysis. 相似文献
66.
Mitochondrial DNA control region polymorphisms: genetic markers for ecological studies of marine turtles 总被引:9,自引:0,他引:9
We describe a rapid and sensitive method for the detection of population-specific genetic markers in mitochondrial DNA (mtDNA) and the use of such markers to analyse population structure of marine turtles. A series of oligonucleotide primers specific for the amplification of the mtDNA control region in Cheloniid turtles were designed from preliminary sequence data. Using two of these primers, a 384–385-bp sequence was amplified from the 5′ portion of the mtDNA control region of 15 green turtles Chelonia mydas from 12 different Indo-Pacific rookeries. Fourteen of the 15 individuals, including some with identical whole-genome restriction fragment patterns, had sequences that differed by one or more base substitutions. Analysis of sequence variation among individuals identified a total of 41 nucleotide substitutions and a 1-bp insertion/deletion. Comparison with evidence from whole-genome restriction enzyme analysis of the same individuals indicated that this portion of the control region is evolving approximately eight times faster than the average rate and that the sequence analysis detected approximately one fifth of the total variation present in the genome. Restriction enzyme analysis of amplified products from an additional 256 individuals revealed significant geographic structuring in the distribution of mtDNA genotypes among five of the 10 rookeries surveyed extensively. Additional geographic structuring of genotypes was identified through denaturing gradient gel electrophoresis (DGGE) of amplified products. Only two of the 10 rookeries surveyed could not be differentiated, indicating that the Indo-Pacific C. mydas include a number of genetically differentiated populations, with minimal female-mediated gene flow among them. Important applications for genetic markers in the conservation and management of marine turtles include the identification of appropriate demographic units for research and management (i.e. genetically discrete populations) and assessment of the composition of feeding and harvested populations. 相似文献
67.
Fourier transform infrared (FTIR) spectroscopy for identifying Lactobacillus species 总被引:3,自引:0,他引:3
Abstract Fourier Transform infrared (FTIR) spectroscopy can be used to identify microorganisms. This study describes the influence of culture conditions on FTIR spectra and the discrimination of Lactobacillus species found in breweries. Fifty three Lactobacillus strains were analysed by FTIR spectroscopy and identification at the species level was correct for 94% of the strains, and at the strain level for 91% of the strains. 相似文献
68.
Jean-François Manen Alessandro Natali Friedrich Ehrendorfer 《Plant Systematics and Evolution》1994,190(3-4):195-211
A phylogenetic analysis of 25 species, representing eight genera of theRubieae tribe (Rubiaceae), has been made using the DNA sequence of the chloroplastatp B-rbc L intergene region. Six tropical genera from other tribes ofRubiaceae have been used as outgroups. Whatever the method of analysis (distance, parsimony or maximum likelihood), five groups are clearly separated and described as informal clades. Their relative relationships are not clearly resolved by the parsimony analysis, resulting in eight equally parsimonious trees, 327 steps long, with a consistency index (CI) of 0.749 (excluding uninformative sites). TheRubieae tribe appears monophyletic from the data available. Some new and partly unexpected phylogenetic relationships are suggested. The genusRubia forms a separate clade and appears to be the relatively advanced sister group of the remaining taxa. TheSherardia clade also includes the generaCrucianella andPhuopsis. Galium sect.Aparinoides appears closely attached to theAsperula sect.Glabella clade. The remaining taxa ofGalium are paraphyletic:Galium sect.Platygalium (in theCruciata clade) is linked to the advanced generaCruciata andValantia; the more apomorphic groups ofGalium form theGalium sect.Galium clade, including the perennial sectionsGalium, Leiogalium, andLeptogalium as well as the annual (and possibly polyphyletic) sect.Kolgyda. 相似文献
69.
Friedrich Ehrendorfer Jean-François Manen Alessandro Natali 《Plant Systematics and Evolution》1994,190(3-4):245-248
Representatives of seven genera from five tribes ofRubiaceae have been compared in respect to a non-coding intergene cpDNA region of about 1000 bp, situated between the atpB and the rbcL genes. The resulting most parsimonious PAUP cladogram corresponds very well with one based on total cpDNA restriction site data obtained byBremer & Jansen (1991). The two different molecular analyses thus corroborate each other and contribute to an improved systematic arrangement of the large family, e.g., in respect to placing the tribeHedyotideae clearly into the subfamilyRubioideae, closer toRubieae than toPsychotrieae. 相似文献
70.
祁连山地区植被特征及其分布规律 总被引:22,自引:0,他引:22
分析和讨论了祁连山地区主要植被类型及其分布特征。祁连山地区随着青藏高原的强烈隆升表现为整体抬升,植被具有明显的生态地理边缘效应特征和高原地带性规律。该区植被虽然受到四周的较大影响,但各类高寒植被占有绝对优势,表现出与青藏高原植被整体明显的相似性和广泛的一致性。另一方面,本区植被也有其特殊性及与高原面存在一些差异。因此,建议把祁连山地区做为青藏高原植被区的次一级独立单元 相似文献