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51.
黄瓜连作土壤酚酸类物质积累对土壤微生物和酶活性的影响 总被引:49,自引:3,他引:46
伴随连作年限的增加,日光温室黄瓜连作土壤中酚酸类物质(对羟基苯甲酸、阿魏酸、苯甲酸)明显积累,连作5~9年的土壤酚酸类物质含量显著高于连作1~3年的土壤.伴随外源酚酸类物质处理浓度的增加,黄瓜根区土壤中细菌、放线菌和微生物总量以及N生理群均呈先升后降趋势,在80 μg·g-1处理浓度下细菌、放线菌数量最多,处理浓度在120μg·g-1以下土壤真菌数量(包括尖孢镰刀菌、疫霉)急剧增长;多酚氧化酶、过氧化氢酶、蔗糖酶、脲酶和蛋白酶活性也同样呈先升后降趋势,但其峰值对应的浓度不同. 相似文献
52.
Kassie F Lhoste EF Bruneau A Zsivkovits M Ferk F Uhl M Zidek T Knasmüller S 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2004,802(1):211-215
Aim of this study was to investigate the impact of intestinal microfloras from vegetarians and non-vegetarians on the DNA-damaging activity of 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ), a carcinogenic heterocyclic amine that is found in fried meats. Floras from four vegetarians (Seventh Day Adventists) and from four individuals who consumed high amounts of meats were collected and inoculated into germfree F344 rats. The rats were kept on isocaloric diets that either contained animal derived protein and fat (meat consumers group) or proteins and fat of plant origin (vegetarian groups). IQ (90 mg/kg bw) was administered orally, after 4 h the extent of DNA-damage in colon and liver cells was determined in single cell gel electrophoresis assays. In all groups, the IQ induced DNA-migration was in the liver substantially higher than in the colon. In animals harbouring floras of vegetarians, the extent of damage was in both organs significantly (69.2% in the liver, P<0.016 and 64.7%, P<0.042 in the colon, respectively) lower than in the meat consumer groups. Our findings show that diet related differences in the microfloras have a strong impact on the genotoxic effects of IQ and suggest that heterocyclic amines are less genotoxic and carcinogenic in individuals that consume mainly plant derived foods. 相似文献
53.
Consumption of milk from transgenic goats expressing human lysozyme in the mammary gland results in the modulation of intestinal microflora 总被引:4,自引:0,他引:4
Lysozyme is a key antimicrobial component of human milk that has several health-promoting functions including the development
of a healthy intestinal tract. However, levels of lysozyme in the milk of dairy animals are negligible. We have generated
transgenic dairy goats that express human lysozyme (HLZ) in their milk in an attempt to deliver the benefits of human milk
in a continual fashion. To test the feasibility of this transgenic approach to achieve a biological impact at the level of
the intestine, feeding trials were conducted in two animal models. Pasteurized milk from HLZ transgenic animals was fed to
both kid goats (ruminant model) and young pigs (human model), and the numbers of total coliforms and Escherichia coli present in the small intestine were determined. Data from this proof-of-principle study demonstrate that milk from transgenic
animals was capable of modulating the bacterial population of the gut in both animal models. Pigs that consumed pasteurized
milk from HLZ transgenic goats had fewer numbers of coliforms and E. coli in their intestine than did those receiving milk from non-transgenic control animals. The opposite effect was seen in goats.
Milk from these transgenic animals not only represent one of the first transgenic food products with the potential of benefiting
human health, but are also a unique model to study the development and role of intestinal microflora on health, well-being
and resistance to disease. 相似文献
54.
Promnuan Yaowanoot Kudo Takuji Chantawannakul Panuwan 《World journal of microbiology & biotechnology》2009,25(9):1685-1689
Actinomycetes are one of the main microbial groups that produce bioactive compounds used as antibiotics. Although bacteria,
mold and yeast have frequently been found in bees, the presence of actinomycetes in bee hives had not been previously identified
or reported. The aim of our research was to focus on the diversity of actinomycetes in bee hives in Thailand. Bees, brood
cells and hive materials were collected from apiaries and natural sources. Thirty-two isolates of actinomycetes were isolated
and identified using morphological, physiological, chemical and molecular characterization. Most of the isolates belonged
to the genus Streptomyces. Some less frequent isolates were classified in the genera Nonomuraea, Nocardiopsis and Actinomadura. 相似文献
55.
56.
西瓜连作障碍及其预防 总被引:5,自引:0,他引:5
<正>我国西瓜种植面积居世界第一,2008年约173.34万公顷,总产量约6282.2万吨。西瓜本身忌连作,连作一年就会有病害发生,需要轮作8年后,才可再种植;连作障碍严重影响了西瓜的生产和市场的供应。 相似文献
57.
急性胰腺炎患者舌苔微生物研究 总被引:6,自引:0,他引:6
目的观察急性胰腺炎(AP)患者舌苔微生物的变化,总结不同舌象、不同病情的AP患者舌苔微生物变化规律。方法将纳入的52例AP患者按分级标准分为轻症(MAP)组(n=33),重症(SAP)组(n=19),分别于治疗前、第3天及第9天清晨观察并采集舌苔,作细菌培养、鉴定、定量,测溶菌酶(LZM)含量。25例健康薄白苔作为对照组。结果AP患者舌苔菌落总数减少。厚苔菌落总数及G-厌氧杆菌多于薄苔,口腔优势菌在厚苔变化表现为厌氧菌检出率增高而需氧菌下降。SAP组肠道杆菌的检出率显著增加;病程早期除口腔链球菌、G+厌氧杆菌减少外,SAP组G-厌氧杆菌也减少;病程第9天时,MAP组基本恢复正常,而SAP组各种异常无改变。AP患者舌苔LZM均显著升高。结论AP病程中口腔舌面出现微生态失调现象,严重程度与病情及舌苔的变化有关。AP厚苔菌落总数多于薄苔,主要为G-厌氧杆菌增加。厚苔厌氧菌增加,而需氧菌减少。 相似文献
58.
Steeped barley was germinated in laboratory scale solid-state fermentors at various oxygen partial pressures. Experiments were performed with sterile living barley and with barley colonized by the natural microflora. On line monitoring of CO2 concentration in the off-gas of the reactors reflected the metabolic activity more accurately than heat production measurement. When colonized by micro-organisms, the respiratory activity of germinating barley increased with the oxygen level in the air. However, CO2 profiles of sterile barley did not alter at different oxygen concentrations in the inlet air of the reactor. It is concluded that the germination phase of malting can be optimized by controlling the oxygen supply to the barley based on CO2 production measurements and taking into account microbial aspects. 相似文献
59.
本文对10名患有腹泻或肠炎的患者和10名健康者做了肠道五种优势菌群的检测,应用模式识别对两组菌群检测值进行分析。结果,图形上两组清楚地分为两处,有明显的分界线。表明,5种菌群数值在两组间具有明显差异。经评估,可知该方法平均诊断预报能力可达75%以上。 相似文献
60.
Hydrogen sulfide (H2S), generated through various endogenous enzymatic and nonenzymatic pathways, is emerging as a regulator of physiological and pathological events throughout the body. Bacteria in the gastrointestinal tract also produce significant amounts of H2S that regulates microflora growth and virulence responses. However, the impact of the microbiota on host global H2S bioavailability and metabolism remains unknown. To address this question, we examined H2S bioavailability in its various forms (free, acid labile, or bound sulfane sulfur), cystathionine γ-lyase (CSE) activity, and cysteine levels in tissues from germ-free versus conventionally housed mice. Free H2S levels were significantly reduced in plasma and gastrointestinal tissues of germ-free mice. Bound sulfane sulfur levels were decreased by 50–80% in germ-free mouse plasma and adipose and lung tissues. Tissue CSE activity was significantly reduced in many organs from germ-free mice, whereas tissue cysteine levels were significantly elevated compared to conventional mice. These data reveal that the microbiota profoundly regulates systemic bioavailability and metabolism of H2S. 相似文献