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排序方式: 共有343条查询结果,搜索用时 15 毫秒
31.
Sanna V Kirschvink N Gustin P Gavini E Roland I Delattre L Evrard B 《AAPS PharmSciTech》2004,5(2):17-23
The purpose of this research was to investigate the effects of processing conditions on the characteristics of solid lipid
microparticles (SLM) with a potential application as carriers for pulmonary administration. Compritol (5.0% wt/wt) SLM dispersions
were prepared by rotor-stator homogenization, at different surfactant concentrations and emulsification times. The SLM were
characterized, in terms of morphology and size, after lyophilization and sterilization by autoclaving process. In vivo assessment
was carried out in rats by intratracheal instillation of either placebo or SLM dispersion, and by bronchoalveolar lavage for
cytological analysis. Mean particle size of 4 to 5 μm was achieved using 0.3% and 0.4% (wt/wt) of emulsifier (Poloxamer 188)
and emulsification times of 2 and 5 minutes. The particles showed spherical shape and smooth surface. The morphology of microparticles,
the size, and the size distribution were not substantially modified after lyophilization and sterilization. Total cell counts
showed no significant differences between placebo and SLM 0.5% or 2.5% groups. Regarding cytology, percentage of polymorphonuclear
neutrophils and macrophages did not significantly differ between groups. These results suggest that a single intratracheal
administration of the SLMs does not induce a significant inflammatory airway response in rats and that the SLMs might be a
potential carrier for encapsulated drug via the pulmonary route. 相似文献
32.
Rebaudo R Melani R Carità F Rosi L Picchio V Ruggeri P Izvarina N Balestrino M 《Neurochemical research》2000,25(11):1493-1495
Intracerebroventricular (ICV) administration of creatine increased cerebral phosphocreatine in normal rats by 67%, the highest increase so far reported in an in vivo model. We used osmotic minipumps (Alzet, Palo Alto, CA, USA) to administer creatine, 0.5mM, to the lateral ventricle at the rate of 10 l/h for 3 days. Brain phosphocreatine in saline-treated controls was 33 ± 17 M/g protein (mean ± SD, N = 9). In creatine-treated rats (0.5 mM for 3 days) such content was 55 ± 17 M/g protein (mean ± SD, N = 7). This difference is statistically significant (p = 0.02, t-test). The increase we found in cerebral phosphocreatine is of an order of magnitude comparable to the increase previously found in in vitro experiments, and may be effective in protecting brain tissue from ischemic damage. 相似文献
33.
The concentrations of cadmium and other metal ions in selected organs, urine, and blood of female rats were measured after
exposure to cadmium chloride through their diet or by oral or intravenous administration. The hematological and urinary variations
were followed for 4 wk.
Body weight gain and the weights of livers and kidneys from all treated groups were not significantly different from the controls.
No gross morphological changes were observed in any of the tissues studied at necropsy.
The accumulation of cadmium occurred in the liver and kidney. The zinc levels in these organs were elevated relative to controls,
in all treated groups regardless of dose and exposure route. Copper was elevated in the liver, kidney, bone, and blood of
animals subject to intravenous administration of cadmium. Hepatic iron was decreased in the dietary and orally treated groups,
but was not affected in the intravenous study group. The level of magnesium in kidney was increased for all exposure routes,
but that of liver was increased only in the intravenously injected groups. The changes in the concentrations of sodium, potassium,
calcium, and phosphorus did not follow a specific pattern and varied from organ to organ, depending on the exposure route.
The discussion includes a relationship between tissue injury and the alteration of tissue essential element concentrations. 相似文献
34.
Effectiveness of weekly subcutaneous recombinant human erythropoietin administration for chemotherapy-induced anemia 总被引:6,自引:0,他引:6
Mamoru Tsukuda Seiichiro Yuyama Hidehiro Kohno Keiko Itoh Toshiyuki Kokatsu Satoshi Kawai 《Biotherapy》1998,11(1):21-25
The effects of weekly subcutaneousrecombinant human erythropoietin (r-hEPO)administration on anemia during chemotherapy includingcisplatin and 5-fluorouracil in patients with head andneck carcinomas were examined. Weekly subcutaneousr-hEPO administration in cancer patients has not beeninvestigated previously. Patients were treated withr-hEPO 100 IU/kg (2 patients), 200 IU/kg (6 patients),or 400 IU/kg (5 patients), or placebo, andeffectiveness was evaluated by monitoring hemoglobinconcentration changes after administration for 8weeks. Hemoglobin concentrations in all 3 r-hEPOdosage groups were higher than that in the controlgroup during chemotherapy. All r-hEPO doses producedimprovements in the anemia induced by chemotherapy;however, the 400 IU/kg dose was most effective. Therequirement for blood transfusions decreased inpatients receiving r-hEPO therapy, and no significantside-effects were associated with r-hEPOadministration. These results suggest thatchemotherapy-induced anemia can be prevented by weeklysubcutaneous r-hEPO administration. 相似文献
35.
辣椒素是从辣椒中提取出来的一种具有镇痛作用的物质。通过激活感觉神经纤维上的瞬时感受器电位香草酸受体1(transient receptor potential vanilloid 1,TRPV1),释放并消耗大量神经肽物质,使神经细胞对伤害性刺激产生脱敏化反应,进而发挥持久的镇痛作用而不影响运动功能。因而在难治性疼痛类疾病中,辣椒素具有独特的治疗价值。以辣椒素为主要成分的制剂已经在临床治疗中开展应用。特定位点注射辣椒素或其类似物resiniferatoxin可以减轻癌痛患者的疼痛症状。但由于辣椒素的治疗剂量与毒性剂量存在部分重叠,使得其在临床应用中受到一定程度的限制。不同的给药方式和作用部位所产生的作用效果可能不同。为深入了解辣椒素的镇痛作用及作用机制,充分发挥其治疗价值,现从不同给药途径总结近几年来辣椒素镇痛作用的研究成果。 相似文献
36.
Testosterone is an important regulator of social–motivational behavior and is known for its dominance-enhancing and social-anxiolytic properties. However, to date no studies have systematically investigated the causal effect of testosterone on actual social approach–avoidance behavior in humans. The present study sets out to test the effects of testosterone administration in healthy female volunteers using an objective implicit measure of social motivational behavior: the social Approach–Avoidance Task, a reaction time task requiring participants to approach or avoid visually presented emotional (happy, angry, and neutral) faces. Participants showed significantly diminished avoidance tendencies to angry faces after testosterone administration. Testosterone did not affect approach–avoidance tendencies to social affiliation (happy) faces. Thus, a single dose testosterone administration reduces automatic avoidance of social threat and promotes relative increase of threat approach tendencies in healthy females. These findings further the understanding of the neuroendocrine regulation of social motivational behavior and may have direct treatment implications for social anxiety, characterized by persistent social avoidance. 相似文献
37.
Chiu-Hsia Chiu Chih-Hsin Cheng Wen-Ren Gua Yuan-Kuang Guu Winton Cheng 《Fish & shellfish immunology》2010,29(6):1053-1059
38.
The present study was conducted to characterize the binding of neurotransmitter receptors (dopamine D(2), serotonin 5-HT(2), histamine H(1), adrenaline alpha(1) and muscarine M(l) receptors) in the rat's brain after the oral administration of haloperidol, risperidone, and olanzapine. Haloperidol at 1 and 3 mg/kg displayed significant activity to bind the D(2) receptor (increase in the Kd value for [(3)H]raclopride binding) in the corpus striatum with little change in the activity toward the 5-HT(2) receptor (binding parameters for [(3)H]ketanserin). In contrast, risperidone (0.1-3 mg/kg) showed roughly 30 times more affinity for the 5-HT(2) receptor than D(2) receptor. Also, olanzapine (1-10 mg/kg) was most active toward the H(1) receptor in the cerebral cortex, corpus striatum, and hippocampus, was less active in binding 5-HT(2) and D(2) receptors, and showed the least affinity for alpha(1) and M(1) receptors. In conclusion, haloperidol and risperidone administered orally selectively bind D(2) and 5-HT(2) receptors, respectively, in the rat brain, while olanzapine binds H(1), 5-HT(2), and D(2) receptors more than alpha(1) and M(1) receptors. 相似文献
39.
Chitosan nanoparticle as gene therapy vector via gastrointestinal mucosa administration: results of an in vitro and in vivo study 总被引:2,自引:0,他引:2
This study was designed to investigate the in vitro and in vivo transfection efficiency of chitosan nanoparticles used as vectors for gene therapy. Three types of chitosan nanoparticles [quaternized chitosan -60% trimethylated chitosan oligomer (TMCO-60%), C(43-45 KDa, 87%), and C(230 KDa, 90%)] were used to encapsulate plasmid DNA (pDNA) encoding green fluorescent protein (GFP) using the complex coacervation technique. The morphology, optimal chitosan-pDNA binding ratio and conditions for maximal in vitro transfection were studied. The in vivo transfection was conducted by feeding the chitosan/pDNA nanoparticles to 12 BALB/C-nu/nu nude mice. Both conventional and TMCO-60% could form stable nanoparticles with pDNA. The in vitro study showed the transfection efficiency to be in the following descending order: TMCO-60%>C(43-45 KDa, 87%)>C(230 KDa, 90%). TMCO-60% proved to be the most efficient and the optimal chitosan/pDNA ratio being 3.2:1. In vivo study showed most prominent GPF expression in the gastric and upper intestinal mucosa. GFP expression in the mucosa of the stomach and duodenum, jejunum, ileum, and large intestine were found, respectively, in 100%, 88.9%, 77.8% and 66.7% of the nude mice examined. TMCO-60%/pDNA nanoparticles had better in vitro and in vivo transfection activity than the other two, and with minimal toxicity, which made it a desirable non-viral vector for gene therapy via oral administration. 相似文献
40.
This study investigated the pharmacokinetic properties of crocin following oral administration in rats. After a single oral dose, crocin was undetected while crocetin, a metabolite of crocin, was found in plasma at low concentrations. Simultaneously, crocin was largely present in feces and intestinal contents within 24h. After repeated oral doses for 6 days, crocin remained undetected in plasma and plasma crocetin concentrations were comparable to the corresponding data obtained after the single oral dose. Furthermore, the absorption characteristics of crocin were evaluated in situ using an intestinal recirculation perfusion method. During recirculation, crocin was undetected and low concentrations of crocetin were detected in plasma. The concentrations of crocin in the perfusate were reduced through different intestinal segments, and the quantities of drug lost were greater throughout the colon. These results indicate that (1) orally administered crocin is not absorbed either after a single dose or repeated doses, (2) crocin is excreted largely through the intestinal tract following oral administration, (3) plasma crocetin concentrations do not tend to accumulate with repeated oral doses of crocin, and (4) the intestinal tract serves as an important site for crocin hydrolysis. 相似文献