全文获取类型
收费全文 | 1631篇 |
免费 | 130篇 |
国内免费 | 45篇 |
出版年
2023年 | 9篇 |
2022年 | 33篇 |
2021年 | 23篇 |
2020年 | 35篇 |
2019年 | 27篇 |
2018年 | 30篇 |
2017年 | 43篇 |
2016年 | 31篇 |
2015年 | 45篇 |
2014年 | 107篇 |
2013年 | 127篇 |
2012年 | 76篇 |
2011年 | 72篇 |
2010年 | 55篇 |
2009年 | 82篇 |
2008年 | 119篇 |
2007年 | 99篇 |
2006年 | 86篇 |
2005年 | 101篇 |
2004年 | 83篇 |
2003年 | 60篇 |
2002年 | 63篇 |
2001年 | 43篇 |
2000年 | 35篇 |
1999年 | 34篇 |
1998年 | 29篇 |
1997年 | 33篇 |
1996年 | 23篇 |
1995年 | 34篇 |
1994年 | 19篇 |
1993年 | 30篇 |
1992年 | 17篇 |
1991年 | 18篇 |
1990年 | 13篇 |
1989年 | 9篇 |
1988年 | 8篇 |
1987年 | 2篇 |
1986年 | 8篇 |
1985年 | 8篇 |
1984年 | 9篇 |
1983年 | 5篇 |
1982年 | 7篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1973年 | 1篇 |
排序方式: 共有1806条查询结果,搜索用时 312 毫秒
11.
Antoine M. Hakim Marie J. Arrieta Bernard A. Cooper† Hanna M. Pappius 《Journal of neurochemistry》1984,42(6):1582-1587
There is considerable debate on the role of folate in CNS function. Recent work indicates that folate deficiency may affect CNS serotonin metabolism, and clinical studies describe many consequences of such a deficiency. On the other hand some workers maintain that folate deficiency alone causes CNS abnormalities. We maintained rats, through dietary deprivation, at folate levels below 4 ng/ml for more than 6 weeks and showed that at that time both their liver and brain folate levels were significantly reduced. We then studied their local cerebral glucose utilization (LCGU) using the [14C]deoxyglucose technique. This method assesses cerebral function by measuring regional metabolic activity. We also determined LCGU in rats given the same diet but replenished with folate (folate control) and in others given free access to commercially available food (normal controls). Our results show that this degree of folate deficiency has no effect on cerebral function. This contrasts with the focal suppression of LCGU we previously reported in a model of vitamin B12 deficiency. 相似文献
12.
Summary There are many contradictory observations on the mechanohydraulic relation of growing higher plant cells and tissues. Graphical analysis of the simultaneous equations which govern irreversible wall yielding and water absorption has made more comprehensive the understanding of this relation when relative growth rate is plotted against turgor pressure. It suggests that some respiration-dependent and auxin sensitive process might regulate the difference of osmotic potential between cells and water source. Based on anatomical and electrophysiological knowledge of the pea stem xylem, we propose the wall canal system as the mechanism of respiration-dependent water uptake which is sensitive to auxin. This system consists of the xylem apoplastic walls, the xylem proton pumps, active solute uptake system and cell membranes. In the simplest case, third-order simultaneous differential equations are involved. Numerical analysis showed that net uptake of solutes enables water to be taken up against an opposing gradient of water potential. The behaviour of this wall canal system describes well the mechano-hydraulic relation of enlarging plant cells and tissues. Recent typical, but incompatible, interpretations of this relation are critically discussed based on our model.Abbreviations V
the volume of enlarging symplast
-
the average extensibility of the wall
- Pi
turgor pressure
- Y
the yield threshold of the wall
- L
the relative hydraulic conductance
-
the solute reflection coefficient of the plasmamembrane
- Ci
the osmotic concentration of the symplast cells
- Cx
the osmotic concentration of the xylem vessels
- Px
hydrostatic pressure in the xylem vessels
- R
the gas constant
- T
absolute temperature
- o
water potential of xylem fluid
- i
water potential of symplast cells 相似文献
13.
Hsieh JJ 《Biometrical journal. Biometrische Zeitschrift》1989,31(3):339-357
"This paper aims to identify net and partial-crude probabilities in the competing-risk life table context, by using probabilistic approaches. Five types of lifelength random variables are defined to formulate these nonidentifiable probabilities. General expressions for net and partial-crude probabilities are first derived under independent risks assumptions. Two sets of explicit formulas for estimating the net and partial-crude probabilities are then derived in terms of the identifiable overall and crude probabilities by making the additional assumption of piecewise uniform distribution of the lifelength random variables. A study of the degree to which nonidentifiability can affect the net and partial-crude probabilities in a variety of situations is developed. An example from cross-sectional studies is employed to illustrate the methodology developed." 相似文献
14.
P. L. Newland N. J. Emptage 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1996,178(6):749-762
Strain acting on the exoskeleton of insects is monitored by campaniform sensilla. On the tibia of a mesothoracic leg of the locust (Schistocerca gregaria) there are three groups of campaniform sensilla on the proximo-dorsal surface. This study analyses the responses of the afferents from one group, their connections with central neurones and their actions during walking.The afferents of the campaniform sensilla make direct excitatory connections with flexor tibiae motor neurones. They also make direct connections with particular spiking local interneurones that make direct inhibitory output connections with the slow extensor tibiae motor neurone.During walking extension movements of the tibiae during stance produce longitudinal tensile forces on the dorsal tibia that peak during mid stance before returning to zero prior to swing. This decline in tension can activate the campaniform sensilla. In turn this would lead to an inhibition of the extensor tibiae motor neurone and an excitation of the flexor tibiae motor neurones. This, therefore, aids the transition from stance to swing. During turning movements, the tibia is flexed and the dorsal surface is put under compression. This can also activate some of campaniform sensilla whose effect on the flexor motor neurones will reinforce the flexion of the tibia. 相似文献
15.
The MC dynamics of an off-lattice all-atom protein backbone model with rigid amide planes are studied. The only degrees of freedom are the dihedral angle pairs of the C-atoms. Conformational changes are generated by Monte Carlo (MC) moves. The MC moves considered are single rotations (simple moves, SM's) giving rise to global conformational changes or, alternatively, cooperative rotations in a window of amide planes (window moves, WM's) generating local conformational changes in the window. Outside the window the protein conformation is kept invariant by constraints. These constraints produce a bias in the distribution of dihedral angles. The WM's are corrected for this bias by suitable Jacobians. The energy function used is derived from the CHARMM force field. In a first application to polyalanine it is demonstrated that WM's sample the conformational space more efficiently than SM's.Abbreviations CPU
Central Processing Unit
- MC
Monte Carlo
- MCD
Monte Carlo Dynamics
- MD
Molecular Dynamics
- RMS
Root-Mean-Square
- RMSD
Root-Mean-Square-Deviation
- SM
Simple Move
- WM
Window Move 相似文献
16.
Computational sequence analysis of matrix metalloproteinases 总被引:12,自引:0,他引:12
Matrix metalloproteinases (MMP) play a cardinal role in the breakdown of extracellular matrix involved in a variety of biological and pathological processes. Research on MMPs has classified and characterized these enzymes according to their matrix substrate specificity, gene and protein domain structure, and regulation of activity and expression. However, the discovery of new MMPs has introduced a need for a more comprehensive and systematic method of classification and quantitative comparison of known and newly discovered members. This study compiles a sequence alignment, constructs a dendrogram, and calculates physical data and homology percentage assignments in order to obtain further insight into MMP structure-function relationships. Thorough analysis of MMP primary sequence domains, physical data patterns, and statistical analysis of sequence homology yields higher resolution in the similarities and differences that group MMP members. 相似文献
17.
How to sample alignments from their posterior probability distribution given two strings is shown. This is extended to sampling alignments of more than two strings. The result is first applied to the estimation of the edges of a given evolutionary tree over several strings. Second, when used in conjunction with simulated annealing, it gives a stochastic search method for an optimal multiple alignment.Correspondence to: L. Allison 相似文献
18.
The human glucagon receptor encoding gene: structure, cDNA sequence and chromosomal localization 总被引:2,自引:0,他引:2
Si Lok Joseph L. Kuijper Laura J. Jelinek Janet M. Kramer Theodore E. Whitmore Cindy A. Sprecher Shannon Mathewes Francis J. Grant Shaula H. Biggs Gary B. Rosenberg Paul O. Sheppard Patrick J. O''Hara Donald C. Foster Wayne Kindsvogel 《Gene》1994,140(2):203-209
Characterization of the human glucagon-receptor-encoding gene (GGR) should provide a greater understanding of blood glucose regulation and may reveal a genetic basis for the pathogenesis of diabetes. A cDNA encoding a complete functional human glucagon receptor (GGR) was isolated from a liver cDNA library by a combination of polymerase chain reaction and colony hybridization. The cDNA encodes a receptor protein with 80% identity to rat GGR that binds [125I] glucagon and transduces a signal leading to increases in the concentration of intracellular cyclic adenosine 3′,5′-monophosphate. Southern blot analysis of human DNA reveals a hybridization pattern consistent with a single GGR locus. In situ hybridization to metaphase chromosome preparations maps the GGR locus to chromosome 17q25. Analysis of the genomic sequence shows that the coding region spans over 5.5 kb and is interrupted by 12 introns. 相似文献
19.
The sex ratio in final-instar larvae of a birch-feeding, free-living solitary sawfly, Dineura virididorsata, was investigated in Finnish Lapland. The prepupal proportion of females, pooled over ten sites, was 56%, and at four individual
sites the sex ratio was significantly female-biased. Larval survival from egg to prepupae did not differ between the sexes.
This suggests a femalebiased primary sex ratio in the field. The sex ratio varied among the sites but not among host trees
within sites. Contrary to previous results with hymenopterans, we did not find that differences in the sex ratio depended
on forage quality: site-specific or tree-specific sex ratios did not correlate with the average prepupal weight. A literature
search indicated that female-biased sex ratios are also common in other free-living sawflies. We are unable to explain sex
ratios of Dineura virididorsata or other free-living sawflies with existing general models. 相似文献
20.
Virginia Muto 《Journal of biological physics》1993,19(2):113-122
A two-dimensional anharmonic model, the so-called Toda-Lennard-Jones model, is considered in order to investigate the problems related to the lifetime of the open states precursors to full denaturation, in inhomogeneous ring-shaped DNA molecules. It is found that a transition from double-stranded to single-stranded DNA occurs locally around physiological temperature. Moreover, the presence of inhomogeneities enhances the hydrogen bond breaking. 相似文献