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11.
Apolipophorin III (apoLp-III) is an exchangeable apolipoprotein that binds to lipopolysaccharides (LPS). Polyacrylamide gel electrophoresis analysis demonstrated that apoLp-III from Galleria mellonella associated with various truncated LPS variants, including lipid A. Subsequent binding studies were performed employing the intrinsic tyrosine fluorescence properties of apoLp-III, which is highly quenched in the unbound state. A marked increase in tyrosine fluorescence intensity was observed upon binding to LPS or detoxified LPS, indicating a new microenvironment for Tyr-142. This also implies that the LPS carbohydrate region is involved in LPS binding. Dissociation constants (Kd) measured by apoLp-III titration were estimated at approximately 1 microM. Increasing the ionic strength did not decrease the Kd, neither did LPS phosphate removal. In addition, truncation apoLp-III mutants, lacking two complete helices, were still able to associate with LPS. This indicates that the association of apoLp-III with LPS may not be governed by charge but by hydrophobic interactions. 相似文献
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Nunzio Esposito Olga G. Ovchinnikova Amalia Barone Astolfo Zoina Otto Holst Antonio Evidente 《化学与生物多样性》2008,5(12):2662-2675
13.
Andrej A. Romanovsky Alexandre A. Steiner Luiz G. S. Branco Ladislav Janský Valery N. Gourine 《Journal of thermal biology》2004,29(7-8):407-411
(1) Administration of arginine vasopressin (AVP) in the ventral septal area (VSA) or intracerebroventricularly (i.c.v.) is thought to attenuate lipopolysaccharide (LPS) or prostaglandin (PG) E2 fevers in rabbits and rats by acting on the V1 receptor. (2) We found that the fever response of rabbits to intravenous LPS (200 ng/kg) or intra-VSA PGE2 (500 ng) was not attenuated but enhanced by intra-VSA AVP (5 μg); a pharmacological analysis showed that this fever-enhancing effect was mediated by the V2 receptor. (3) The febrile response of rats to intraperitoneal (50 μg/kg) or i.c.v. (100 ng) LPS was unaffected by i.c.v. AVP (2.5–100 ng). (4) The role of AVP in fever should be re-examined. 相似文献
14.
Clark M. Blatteis Shuxin Li Zhonghua Li Vit Perlik Carlos Feleder 《Journal of thermal biology》2004,29(7-8):369-381
(1) Cobra venom factor (CVF)-induced hypocomplementemia dose-dependently attenuates the febrile responses of guinea pigs and mice to intraperitoneally (ip) but not to intravenously (iv) injected endotoxic bacterial lipopolysaccharide (LPS). (2) Iv but not ip LPS causes fever in complement component 3 (C3) gene-ablated mice, but neither iv nor ip LPS evokes a body core temperature (Tc) rise when WT and these mice's C5a receptors type 1 are blocked. C5 knockout mice also do not develop fever following either iv or ip LPS. C5a thus appears to be a critical mediator of LPS fever. (3) C5 knockouts develop fever in response to intracerebroventricularly (icv) injected LPS or prostaglandin (PG)E2; the site of action of C5a is therefore peripheral rather than central. (4) The initiation of the febrile responses to both iv and ip LPS is temporally correlated with the appearance of LPS in the liver's Kupffer cells (Kc). (5) PGE2 is released by liver in immediate response to the injection of CVF into the portal vein of anesthetized guinea pigs; its level rises quickly to its maximum. LPS injected similarly also evokes a quick release of PGE2 from the liver; it, however, is prevented by prior hypocomplementation. (6) Neither LPS nor IL-1β induces PGE2 release from Kc in vitro within the first hour after treatment, but serum C and C plus LPS or IL-1β very quickly trigger PGE2 increases of similar magnitudes, catalyzed non-differentially by cyclooxygenase (COX)-1 and COX-2. Kc would thus appear to be the principal site of action of C5a, inducing the release of PGE2. (7) PGE2 is detectable in the plasma of conscious guinea pigs in temporal correlation with the onset of the Tc rise following ip LPS; cytokines appear significantly later. (8) Taken together, these results indicate that LPS-activated C, rather than LPS or IL-1β by itself, triggers PGE2 release by Kc. This PGE2 could be the factor that stimulates vagal afferents, thereby providing the signal to the brain that mediates the febrile response. 相似文献
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脂多糖对血管平滑肌细胞一氧化氮合酶基因表达及细胞增殖的影响 总被引:1,自引:0,他引:1
应用RNA印迹分析和亚硝酸盐含量测定检查脂多糖(LPS)对大鼠血管平滑肌细胞(VSMC)一氧化氮合酶(NOS)基因表达及NO合成的影响,用3H-TdR参入实验观察LPS对细胞DNA合成的影响.结果表明,LPS在诱导VSMCiNOSmRNA表达和促进NO合成的同时,抑制VSMCDNA合成.证明LPS的作用与其浓度和作用时间有关 相似文献
17.
L. P. T. M. Zevenhuizen Ineke Scholten-Koerselman M. A. Posthumus 《Archives of microbiology》1980,125(1-2):1-8
Hot phenol-water extractions were carried out of cells from 12 strains of the fast-growing rhizobia Rhizobium leguminosarum, Rhizobium phaseoli, Rhizobium trifolii and Rhizobium meliloti. Purified lipopolysaccharide preparations contain neutral sugars, hexosamines, 2-keto-3-deoxyoctonate and uronic acids. Glucose, galactose, mannose, rhamnose and fucose are present in the majority of the LPS-preparations, but in varying proportions. Heptose was only found in some of them. O-methylated sugars are present in small amounts is most preparations, the kind of sugar being characteristic for lipopolysaccharides from different species. The lipid A part of lipopolysaccharides from all strains examined has identical patterns of fatty acids, namely -OH-C14:0, -OH-C15:0 (anteiso branched), -OH-C16:0 and -OH-C18:0. Comparison of the total compositions of Rhizobium lipopolysaccharides shows many differences among different species as among strains of a single species. Nearly identical lipopolysaccharide compositions also exist among certain strains, which constitute the same chemotype and which are also immunologically related. In view of a possible role of surface carbohydrates of Rhizobium in the root nodule symbiosis, the specificity of the binding of legume lectins with exo- and lipopolysaccharides of Rhizobium is discussed.Non-Standard Abbreviations LPS
lipopolysaccharide(s)
- EPS
exopolysaccharides(s)
- cetavlon
cetyltrimethylammoniumbromide
- KDO
2-keto-3-deoxyoctonate
- ECL
equivalent chain length
Part II on Surface Carbohydrates of Rhizobium 相似文献
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Lipopolysaccharides (LPS) from Corynebacterium autotrophicum were isolated and analyzed. Autotrophically grown cells contained 2–5 mg of partly purified LPS per g dry weight of lyophilized cells. Serological cross reaction with Lipid A antigen of Salmonella minnesota confirmed the presence of LPS in C. autotrophicum. Electron microscopy of negatively stained Polymyxin B-treated cells showed formation of blebs on the Outer Membrane indicating an interaction of Polymyxin B specifically with LPS. Up to now, no Gram-positive organisms are known which contain any LPS. Thus, C. autotrophicum, though giving opposite results when the Gram-staining reaction was applied by several authors, has to be classified into the group of Gram-negative bacteria.Non-Common Abbreviations LPS
lipopolysaccharide
- KDO
2-keto-3-deoxyoctonate 相似文献
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